Evaluate the effect of salicylic acid and ascorbic acid on agronomic and antioxidant activities of Sunflower in water deficit conditions

Shilan  Davodi; Bahram    Mirshekari; Touraj Mir Mahmoudi; Farhad Farahvash; Saman Yazdan Seta

doi:10.14719/pst.1428

Authors

Shilan Davodi Department of Agronomy, Tabriz Branch, Islamic Azad University, Tabriz, Iran Tabriz, 5153715898, Iran https://orcid.org/0000-0001-8639-8299
Bahram Mirshekari Department of Agronomy, Tabriz Branch, Islamic Azad University, Tabriz, Iran Tabriz, 5153715898, Iran. https://orcid.org/0000-0003-2762-8079
Touraj Mir Mahmoudi Department of Agriculture, Tabriz Branch, Islamic Azad University, Mahabad 433-59135, Iran https://orcid.org/0000-0003-2166-3834
Farhad Farahvash Department of Agronomy, Tabriz Branch, Islamic Azad University, Tabriz, Iran Tabriz, 5153715898, Iran. https://orcid.org/0000-0002-8892-419X
Saman Yazdan Seta Department of Agriculture, Tabriz Branch, Islamic Azad University, Mahabad 433-59135, Iran https://orcid.org/0000-0003-3220-5118

DOI:

https://doi.org/10.14719/pst.1428

Keywords:

Antioxidants, Catalase, Oil percent, Sunflower

Abstract

To evaluate the effect of salicylic acid and ascorbic acid foliar application on a number of agronomic and antioxidant activities of sunflower, an experiment was conducted at research farm of Mahabad city, locted in the south of West Azerbaijan Province, Iran during 2016-2018 cropping seasons. The experiment was laid out as a split-plot design based on a completely randomized block design with three replications. Irrigation regimes (irrigation after 60 mm and irrigation after 120 mm evaporation from class A pan respectively) were established as main plots and the foliar applications of ascorbic acid (control, 50 and 100 ppm) and salicylic acid (control, 100 and 200 ppm) were the sub-plots. In the present study, the highest amount of proline content and activity of antioxidant enzymes was recorded under water deficit conditions and foliar application of 100 ppm salicylic acid. Applying salicylic acid increased grain yield under both normal irrigation and water deficit conditions. Also, the highest proline content and activity of antioxidant enzymes were under the treatment of water deficit and application of 50 ppm ascorbic acid. The results showed no significant difference between the combined foliar application of salicylic acid and ascorbic acid in terms of grain yield and oil percentage with their separate application. Therefore, simultaneous use of these two substances under normal irrigation and water deficit conditions was not economical and not recommended.

Downloads

Download data is not yet available.

References

Amirian SH, Golparvar AR, Nassiri B. Character association, regression and path analysis in sunflower (Helianthus annuus L.) hybrids. Tech J Eng App Sci. 2013;3(24):3640-43.

Huseynova IM, Suleymanov SY, Aliyev JA. Structural functional state of thylakoid membranes of wheat genotypes under water stress. https://doi.org/10.1016/j.bbabio.2007.01.014.

Papadakis AK, Roubelakis-Angelakis KA. Polyamines inhibit NADPH oxidasemediated superoxides generation and putrescine prevents programmed cell death syndrome induced by the polyamine oxidase generated hydrogen peroxide. Planta. 2005;220:826-37. https://doi.org/10.1007/s00425-004-1400-9

Foyer C, Noctor G. Oxygen processing in photosynthesis: regulation and signalling. New Phytologist. 146(3): 2002;359-88. https://doi.org/10.1046/j.1469-8137.2000.00667.x

Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 2002; 7:405-10. DOI: 10.1016/s1360-1385(02)02312-9.https://doi.org/10.1016/S1360-1385(02)02312-9

Yildiz Aktas L, Turkyilmaz B, Akca H, Parlak S. Role of abscisic acid and proline treatment on induction of antioxidant enzyme activities and drought tolerance responses of Laurus nobilis L. Seedlings. Fen. Bilimleri Dergisi. 2007;28:14-27.

Farooq M, Wahid A, Lee DJ, Cheema SA, Aziz T. Comparative time course action of the foliar applied glycinebetaine, salicylic acid, nitrous oxide, brassinosteroids and spermine in improving drought resistance of rice. Journal of Agronomy and Crop Science. 2010; 196:336-45. https://doi.org/10.1111/j.1439-037X.2010.00422.x

Gallie DR. L-ascorbic acid: a multifunctional molecule supporting plant growth and development. Scientifica. 2013;1-24. https://doi.org/10.1155/2013/795964.

Hara M, Furukawa J, Sato A, Mizoguchi T, Miura K. Abiotic stress and role of salicylic acid in plants in abiotic stress responses in plants. editors A. Parvaiza and MNV Prasad (New York, NY: Springer). 2012;235-51. https://doi.org/10.1007/978-1-4614-0634-1_13

Miura K. Tada Y. Regulation of water, salinity and cold stress responses by salicylic acid. Frontiers in Plant Science. 2014;5(4):1-12. https://doi.org/10.3389/fpls.2014.00004

Younis ME, Hasaneen MNA, Kazamel AMS. Exogenously applied ascorbic acid ameliorates detrimental effects of NaCl and mannitol stress in Vicia faba seedlings, Protoplasma. 2010;239(1-4):39-48. doi: 10.1007/s00709-009-0080-5.https://doi.org/10.1007/s00709-009-0080-5

Khana W, Prithiviraj B, Smith DL. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology. 2003;160:485-92. https://doi.org/10.1078/0176-1617-00865.

Hegazi AM, El-Shraiy AM. Impact of salicylic acid and paclobutrazol exogenous application on the growth, yield and nodule formation of common bean. Australian Journal of Basic and Applied Sciences. 2007; 1(4): 834-40.

El-Shraiy AM, Hegazi AM. Effect of acetylsalicylic acid, indole-3- bytric acid and gibberellic acid on plant growth and yield of pea (Pisum sativum L.), Australian Journal of Basic and Applied Sciences. 2009;3(4):3514-23.

Khafaga HS, Raeefa AH, Hala MM, Alaa SA. Response of two faba bean cultivars to application of certain growth regulators under salinity stress condition at Siwa Oasis: 1- Growth traits, yield and yield components. Proceedings of the 4th Conference on Recent Technologies in Agriculture. 2009; Nov. 3-4, 236-49. http://conf2009.agr.cu.edu.eg/volum2/27.pdf

Thomson T, Patel GS, Thakar JB, Pandya KS. Effect of foliar application of acetyl salicylic acid and ascorbic acid on protein content, yield and economics of garden pea (Pisum sativum L. cv. Bonneville). International Journal of Current Microbiology and Applied Science. 2017; 6:1987-90. https://doi.org/10.20546/ijcmas.2017.603.233.

Farjam S, Siosemardeh A, Kazemi-Arbat H, Yarnia M, Rokhzadi A. Response of chickpea (Cicer arietinum L.) to exogenous salicylic acid and ascorbic acid under vegetative and reproductive drought stress conditions. Journal of Applied Botany and Food Quality. 2014; 87:80-86. DOI:10.5073/JABFQ.2014.087.012

Britton C, Mehley A. Assay of catalase and peroxidase. Methods in Enzymology. 1955;2:764-75. https://doi.org/10.1016/S0076-6879(55)02300-8

Chance B, Maehly AC. Assays of Catalases and Peroxidases. In: Methods in Enzymology. Colowick SP, Kaplan NO. editors.) Academic Press, New York. 1955; .II. pp. 764- 75. https://doi.org/10.1016/S0076-6879(55)02300-8

Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman J, Sybesma C, Van Montagu M, Inzé D. Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. The EMBO Journal. 1991;10:1723-32. https://doi.org/10.1002/j.1460-2075.1991.tb07696.x

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for waterstress studies. Plant and Soil. 1973;39:205-07. https://doi.org/10.1007/BF00018060

Ashraf M, Foolad MR. Roles of glycinebetaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany. 2007;59(2):206-16. https://doi.org/10.1016/j.envexpbot.2005.12.006

Hussein MM, Balbaa LK, Gaballah MS. Salicylic acid and salinity effects on growth of maize plants. Res J Agric Biol Sci. 2007;3:321-28.

Nazar R, Umar S, Khan NA, Sareer O. Salicylic acid supplementation improves photosynthesis and growth inmustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany. 2015;98:84-94. https://doi.org/10.1016/j.sajb.2015.02.005

Umebese CE, Olatimilehin TO, Ogunsusi TA. Salicyclic acid protects nitrate reductase activity, growth and proline in amaranth and tomato plants during water deficit. American Journal of Agricultural and Biological Sciences. 2009;4:224-29. DOI:10.3844/ajabssp.2009.224.229

Al-Hakimi AMA, Hamada AM. Counteraction of salinity stress on wheat plants by grain soaking in ascorbic acid, thiamin or sodium salicylate. Biologia Plantarum. 2001; 44:253-61. DOI:10.1023/A:1010255526903

Dolatabadian A, Sanavy SAMM, Chashmi NA. The effects of foliar application of ascorbic acid (Vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of Canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomi and Crop Science. 2008;194(3):206-13. https://doi.org/10.1111/j.1439-037X.2008.00301.x

Fu J, Huang B. Involvement of antioxidants and lipid peroxidant in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany. 2001;45:105-14. https://doi.org/10.1016/S0098-8472(00)00084-8

Ushimaru T, Ogawa K, Ishida N, Shibasaka M, Kanematsu S, Asada K, Tsuji H. Changes in organelle superoxide dis- mutase isozymes during air adaptation of submerged rice seedlings: differential behaviour of isozymes in plastids and mitochondria. Planta.1995; 112: 606-13. https://doi.org/10.1007/BF00203662

Abbasi A, Shekari F, Mustafavi SH. Effect of paclobutrazol and salicylic acid on antioxidants enzyme activity in drought stress in wheat. IDESIA, (Chile). 2015;33:5-13. doi.org/10.4067/S0718-34292015000400002.

Manivannan P, Jaleel CA, Kishorekumar A, Sankar B, Somasundaram R, Sridharan R, Panneerselvam R. Changes in antioxidant metabolism of Vigna unguiculata (L.) Walp. by propiconazole under water deficit stress. Coll Surf B: Bioin. 2007;57:69-74. DOI: 10.1016/j.colsurfb.2007.01.004.

Athar HR, Khan A, Ashraf M. Inducing salt tolerance in wheat by exogenously applied ascorbic acid through different modes. J Plant Nutr. 2009;32:1799-1817. https://doi.org/10.1080/01904160903242334

Hafez YM, Bacsó R, Király Z, Künstler A, Király L. Up-regulation of antioxidants in tobacco by low concentrations of H2O2 suppresses necrotic disease symptoms. Phytopathol. 2012;102:848-56. https://doi.org/10.1094/PHYTO-01-12-0012-R

Quan LG, Zhang B, Shi WW, Li HY. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol. 2008;50(1):2-18. doi: 10.1111/j.1744-7909.2007.00599. x.

Kadioglu A, Saruhan N, Sa?lam A, Terzi R, Acet T. Exogenous salicylic acid alleviates effects of long term drought stress and delays leaf rolling by inducing antioxidant system. Plant Growth Regul. 2011; 64:27-37. DOI:10.1007/s10725-010-9532-3.

Saruhan N, Saglam A, Kadioglu A. Salicylic acid pretreatment induces drought tolerance and delays leaf rolling by inducing antioxidant systems inmaize genotypes. Acta Physiol Plant. 2012;34:97-106. https://doi.org/10.1007/s11738-011-0808-7

Farooq S, Shahid M, Khan M B, Hussain M, Farooq M. Improving the productivity of bread wheat by good management practices under terminal drought. J Agro Crop Sci. 2015;201:173-88. https://doi.org/10.1111/jac.12093

Hafez EM, Gharib HS. Effect of exogenous application of ascorbic acid on physiological and biochemical characteristics of wheat under water stress. International Journal of Plant Production. 2016;10(4):579-96.

Rasheed SMS. Effect of salicylic and ascorbic acid on growth, green yield of two broad bean cultivars (Vicia faba L.). ZANCO Journal of Pure and Applied Sciences. 21018; 30 (5):71-88.

Qasim M, Aziz M, Nawaz F, Arif M. Role of salicylic acid and ascorbic acid in alleviating the harmful effects of water stress in Maize (Zea mays L.). Asian J Agric and Biol. 2019;7(3):442-49.

Nazariyan G, Mehrpooyan M, Khiyavi M. Study of effects of drought stress on yield and yield components of four sunflower cultivars in Zanjan, Iran. Plant Ecophysiology. 2009; 3:135-39.

Balo?lu M C, Kavas M, Aydin G, Öktem H A, Yücel A. M. Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress, Turk J Bot. 2012;36:707-14. doi:10.3906/bot-1111-20.

Farooq MA, Wahid N, Kobayashi, D, Fujita S, Basra SMA. Plant drought stress: effects, mechanisms and management. Agron Sustain Dev. 2009; 29:185-212. https://doi.org/10.1051/agro:2008021

Monjezi F, Vazini F, Hassanzadehdelouei M. Effects of iron and zinc spray on yield and yield components of wheat (Triticum aestivum L.) in drought stress. Cercet?ri Agronomice în Moldova. 2013;5(1):153-63. https://doi.org/10.2478/v10298-012-0072-z

Rezaei-Chiyaneh E. Effect of salicylic acid on yield, component yield and essential oil of black cumin (Nigella sativa L.) under Water Deficit Stress. ?Field Crops Research. 2015;12(3):427-37.

Aziz M, Ashraf M, Javaid MM. Enhancement in cotton growth and yield using novel growth promoting substances under water limited conditions. Pak J Bot. 2018; 50:1691-1701.

Delfine S, Tognettir R, Loreto F, Alvino A. Physiological and growth responses to water stress in field grown bell pepper (Capsicum annuum L.). Journal of Horticultural Science and Biotechnology. 2002; 77 (6): 697–704. https://doi.org/10.1080/14620316.2002.11511559

IdreesM, Khan MMA, Aftab T, Naeem M, Hashmi N. Salicylic acid induced physiological and biochemical changes in lemongrass varieties under water stress. Journal of Plant Interaction. 2010;5(4):293-303. https://doi.org/10.1080/17429145.2010.508566

Reda F, Abdel-Rahim EA, El-Baroty GSA, Ayad HA. Response of essential oils, phenolic components and polyphenol oxidase activity of thyme (Thymus vulgaris L.) to some bioregulators and vitamins. International Journal of Agriculture and Biology. 2005; 7(5): 735-39.

Said-Al Ahl HAH, El Gendy AG, Omer EA. Effect of ascorbic acid, salicylic acid on coriander productivity and essential oil cultivated in two different locations. Advances in Environmental Biology. 2014;8(7):2236-50.