Rosmarinic acid production in cell suspension cultures of Ehretia asperula Zollinger & Moritz
DOI:
https://doi.org/10.14719/pst.1490Keywords:
Callus induction, cell suspension culture, Rosmarinic acidAbstract
Plant cell cultures provide an alternative means for producing secondary compounds in food, cosmetic and pharmaceutical industries. Ehretia asperula Zollinger & Moritzi is used as a traditional medicine for the treatment of liver detoxification, ulcers, tumors, inflammation and enhancing the body's resistance in Vietnam. The study was carried out to select suitable callus line for cell suspension cultures of E. asperula Zollinger & Moritzi and investigate the effects of inoculum size, rotation speed and naphthalene acetic acid (NAA) on the proliferation of cell suspension cultures. In addition, the influence of light intensity on the growth and rosmarinic acid (RA) biosynthesis of cell suspension was also surveyed. After 4 weeks of culture, the white to pale yellow friable callus expanded significantly with a fresh weight (FW) of 0.788 g and a high RA content of 2.062 mg/g FW. An appropriate medium for cell proliferation was the liquid B5 medium, which contained 30 g/l glucose, 0.1 mg/l benzyl adenine (BA) and 0.4 mg/l NAA. The results also demonstrated that a 1:20 ratio (w/v) inoculum size, darkness and rotation speed of 90 rpm were the optimal conditions for the proliferation and RA accumulation to 188.217 mg/l in 4 weeks of culture. These findings showed that E. asperula Zollinger & Moritzi cell suspension cultures could be a potential alternative approach for RA production in vitro.
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Chandran H, Meena M, Barupal T, Sharma K. Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnol Rep. 2020;26: e00450. https://doi.org/10.1016/j.btre.2020.e00450
Li YC, Tao WY, Cheng L. Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor. Appl Microbiol Biotechnol. 2009;83(2):233-39. https://doi.org/10.1007/s00253-009-1856-4
Yazaki K. Lithospermum erythrorhizon cell cultures: Present and future aspects. Plant Biotechnol. 2017;34(3):131-42. https://doi.org/10.5511/plantbiotechnology.17.0823a
Titova MV, Popova EV, Konstantinova SV, Kochkin DV, Ivanov IM, Klyushin AG, Titova EV, Nebera EA, Vasilevskaya ER, Tolmacheva GS, Kotenkova EA, Nosov AM, Paek KY. Suspension cell culture of Dioscorea deltoidea–A renewable source of biomass and furostanol glycosides for food and pharmaceutical industry. Agronomy. 2021;11(2):394. https://doi.org/10.3390/agronomy11020394
Khojasteh A, Mirjalili MH, Palazon J, Eibl R, Cusido RM. Methyl jasmonate enhanced production of rosmarinic acid in cell cultures of Satureja khuzistanica in a bioreactor. Eng Life Sci. 2016;16(8):740-49. https://doi.org/10.1002/elsc.201600064
Bhatt R, Mishra N, Bansal PK. Phytochemical, pharmacological and pharmacokinetics effects of rosmarinic acid. J Pharm Sci Innov. 2013;2(2):28-34. https://doi.org/10.7897/2277-4572.02215
Nadeem M, Imran M, Gondal TA, Imran A, Shahbaz M, Amir RM, Sajid MW, Qaisrani TB, Atif M, Hussain G, Salehi B, Ostrander EA, Martorell M, Sharifi-Rad J, Cho WC, Martins N. Therapeutic potential of rosmarinic acid: A comprehensive review. Appl Sci. 2019;9(15):3139. https://doi.org/10.3390/app9153139
Hoa HQ, Khanh TC. Botanical characteristics of three medicinal plants belonging to the Ehretia genus, in the Boraginaceae family. Journal of Medicinal Materials. 2009;14(3):137-41 [In Vietnamese]. Available from: http://vienduoclieu.org.vn/tap-chi/dac-diem-thuc-vat-cua-ba-loai-cay-thuoc-thuoc-chi-cuom-rung-ehretia-p-br-ho-voi-voi-boraginaceae-tap-chi-duoc-lieu-tap-14-so-3-2009-1196_2090
Viet TD, Xuan TD, Van TM, Andriana Y, Rayee R, Tran HD. Comprehensive fractionation of antioxidants and GC-MS and ESI-MS fingerprints of Celastrus hindsii leaves. Medicines. 2019;6(2):64. https://doi.org/10.3390/medicines6020064
Ly TN, Shimoyamada M, Yamauchi R. Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth. leaves and their antioxidative activity. J Agric Food Chem. 2006;54(11):3786-93. https://doi.org/10.1021/jf052743f
Ly TN. Separation process of rosmarinic acid and their derivatives from Celastrus hindsii Benth. leaves. VJST. 2016;54(2C):380-87. https://doi.org/10.15625/2525-2518/54/2C/11865
Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 1968;50:151-58. https://doi.org/10.1016/0014-4827(68)90403-5
Öztürk M, Duru ME, ?nce B, Harmandar M, Topçu G. A new rapid spectrophotometric method to determine the rosmarinic acid level in plant extracts. Food Chem. 2010;123(4):1352-56. https://doi.org/10.1016/j.foodchem.2010.06.021
Sundram TC, Annuar MSM, Khalid N. Optimization of culture condition for callus induction from shoot buds for establishment of rapid growing cell suspension cultures of mango ginger (Curcuma mangga). Aust J Crop Sci. 2012;6(7): 1139-46. Available from: http://www.cropj.com/sundram_6_7_2012_1139_1146.pdf
Haida Z, Syahida A, Ariff SM, Maziah M, Hakiman M. Factors affecting cell biomass and flavonoid production of Ficus deltoidea var. kunstleri in cell suspension culture system. Sci Rep. 2019;9(1):9533. https://doi.org/10.1038/s41598-019-46042-w
Saad NC, Mazlan FI, Karim KA. Factors affecting the establishment and growth of Pogostemon cablin cell suspension cultures. IJARSET. 2016;3(10):2790-96. Available from: http://www.ijarset.com/upload/2016/october/9_IJARSET_khiriah.pdf
Tram PTM, Tien LTT. Studing on growth of strawberry (Fragaria ananassa L.) cell suspension cultures for anthocyanin production. STDJ. 2012;15(2):69-77. https://doi.org/10.32508/stdj.v15i2.1802
Thanh NT, Murthy HN, Yu KW, Jeong CS, Hahn EJ, Paek KY. Effect of inoculum size on biomass accumulation and ginsenoside production by large-scale cell suspension cultures of Panax ginseng. J Plant Biotechnol. 2004;6(4):265-68. Available from: https://www.koreascience.or.kr/article/JAKO200420828098816.kr
Singh M, Chaturvedi R. Evaluation of nutrient uptake and physical parameters on cell biomass growth and production of spilanthol in suspension cultures of Spilanthes acmella Murr. Bioprocess Biosyst Eng. 2012;35(6):943-51. https://doi.org/10.1007/s00449-012-0679-3
Jalil M, Annuar MSM, Tan BC, Khalid N. Effects of selected physicochemical parameters on zerumbone production of Zingiber zerumbet Smith cell suspension culture. eCAM. 2015:1-7. https://doi.org/10.1155/2015/757514
Tan S, Radzali M, Arbakariya A, Mahmood M. Effect of plant growth regulators on callus, cell suspension and cell line selection for flavonoid production from pegaga (Centella asiatica L. Urban). Am J Biochem Biotechnol. 2010;6(4):284-99. https://doi.org/10.3844/ajbbsp.2010.284.299
Phua QY, Chin CK, Asri ZRM, Lam DYA, Subramaniam S, Chew BL. The callugenic effects of 2, 4-dichlorophenoxy acetic acid (2, 4-D) on leaf explants of Sabah snake grass (Clinacanthus nutans). Pak J Bot. 2016;48(2):561-66. Available from: http://www.pakbs.org/pjbot/PDFs/48(2)/18.pdf
Smetanska I. Production of secondary metabolites using plant cell cultures. Adv Biochem. Engin./Biotechnol. 2008;111:187-228. https://doi.org/10.1007/10_2008_103
Hakkim FL, Kalyani S, Essa M, Girija S, Song H. Production of rosmarinic acid in Ocimum sanctum (L.) cell suspension cultures by the influence of growth regulators. Int J Biol Med. Res. 2011;2(4):1158-61. Available from: https://www.researchgate.net/publication/277309487
Soontornchainaksaeng P, Chaicharoen S, Sirijuntarut M, Kruatrachue M. In vitro studies on the effect of light intensity on plant growth of Phaius tankervilliae (Banks ex L’Herit.) Bl. and Vanda coerulea Griff. Science Asia. 2001;27(4):233-37. https://doi.org/10.2306/scienceasia1513-1874.2001.27.233
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