Skip to main navigation menu Skip to main content Skip to site footer

Review Articles

Vol. 13 No. sp9 (2026): Recent Advances in Agriculture

Traditional and modern approaches of root knot nematodes management in vegetable crops: A review

DOI
https://doi.org/10.14719/pst.15643
Submitted
21 May 2026
Published
27-10-2026

Abstract

Vegetables are the major food source for nutritional security for the teeming millions across the globe. Unfortunately, the growth and development of vegetable crops face yield reduction due to the infestation of notorious sedentary endoparasites, root-knot nematodes (RKN, Meloidogyne spp.). The RKN substantially harm the vegetable crops (20–100 %) culminating in huge economic losses to the growers, becoming a serious threat to the world agricultural sector. Chemical-based nematicides are effective to a certain extent but are costly, unsafe for humans and animals and subject to various regulations. Hence, it is crucial to use green and sustainable approaches for managing RKN infection. Approaches such as cultural practices, application of cover/antagonistic crops, use of organic amendments, nanoparticles (NPs) and chemical nematicides have been deployed for management of RKN. However, application of biocontrol agents is a safer, more reliable, cost-effective and promising alternative method for management of RKN. These biocontrol agents not only improve plant health but also facilitate plants to withstand various biotic stresses by inducing systemic resistance. Novel approaches, such as transgenic technologies, omics-based strategies, and integrated management of root-knot nematodes (RKNs), are receiving increasing attention.Hence, the present review focuses on the challenges associated with RKN management and provides explicit information on traditional and modern approaches of RKN management. It also emphasises sustainable biocontrol of RKN through fungi and bacteria, for food security.

References

  1. 1. Gowda MT, Sellaperumal C, Rai AB, Singh B. Root knot nematodes menace in vegetable crops and their management in India: A review. Veg Sci. 2019;46:1–16. https://doi.org/10.61180/aszcm525
  2. 2. Ayaz M, Ali Q, Farzand A, Khan AR, Ling H, Gao X. Nematicidal volatiles from Bacillus atrophaeus GBSC56 promote growth and stimulate induced systemic resistance in tomato against Meloidogyne incognita. Int J Mol Sci. 2021;22:5049. https://doi.org/10.3390/ijms22095049
  3. 3. Ayaz M, Zhao JT, Zhao W, Chi YK, Ali Q, Ali F, et al. Biocontrol of plant parasitic nematodes by bacteria and fungi: A multi-omics approach for the exploration of novel nematicides in sustainable agriculture. Front Microbiol. 2024;15:1433716. https://doi.org/10.3389/fmicb.2024.1433716
  4. 4. Chaudhary KK, Meghvansi MK, editors. Sustainable management of nematodes in agriculture. Vol. 1, Organic management. Cham: Springer; 2022. 440 p. https://doi.org/10.1007/978-3-031-09943-4
  5. 5. Migunova VD, Sasanelli N. Bacteria as biocontrol tools against phytoparasitic nematodes. Plants (Basel). 2021;10:389. https://doi.org/10.3390/plants10020389
  6. 6. Poonia AK, Koul B, Kajla S, Mishra M, Rabbee MF. Grafting tomato scions on root-knot nematode-resistant brinjal rootstocks complemented with biocontrol agents as an integrated nematode management strategy for the development of RKN-resistant tomato. Pathogens. 2025;14:1257. https://doi.org/10.3390/pathogens14121257
  7. 7. Ali Q, Yu C, Wang Y, Sheng T, Zhao X. High killing rate of nematodes and promotion of rice growth by synthetic volatiles from Bacillus strains due to enhanced oxidative stress response. Physiol Plant. 2023;175:e13868. https://doi.org/10.1111/ppl.13868
  8. 8. Abad P, Favery B, Rosso MN, Castagnone-Sereno P. Root-knot nematode parasitism and host response: Molecular basis of a sophisticated interaction. Mol Plant Pathol. 2003;4:217–24. https://doi.org/10.1046/j.1364-3703.2003.00170.x
  9. 9. Sikora RA, Fernandez E. Nematode parasites of vegetables. In: Luc M, Sikora RA, Bridge J, editors. Plant parasitic nematodes in subtropical and tropical agriculture. Wallingford: CAB International; 2005. p. 319–92. https://doi.org/10.1079/9780851997278.0319
  10. 10. Hunt DJ, Handoo ZA. Taxonomy, identification and principal species. In: Perry RN, Moens M, Starr JL, editors. Root-knot nematodes. Wallingford: CAB International; 2009. p. 55–67. https://doi.org/10.1079/9781845934927.0055
  11. 11. Lankubee MC, Sumita K, Singh YA, Patil A. Management of root-knot nematodes in peas using bacterial and fungal bio-agents: A review. Int J Curr Microbiol Appl Sci. 2024;13:102–12. https://doi.org/10.20546/ijcmas.2024.1303.008
  12. 12. Pires D, Vicente CSL, Menéndez E, Faria JMS, Rusinque L, Camacho MJ, et al. The fight against plant-parasitic nematodes: Current status of bacterial and fungal biocontrol agents. Pathogens. 2022;11:1178. https://doi.org/10.3390/pathogens11101178
  13. 13. Mokrini F, Laasli SE, Ezrari S, Belabess Z, Lahlali R. Plant-parasitic nematodes and microbe interactions: A biological control perspective. In: Chaudhary KK, Meghvansi MK, Siddiqui S, editors. Sustainable management of nematodes in agriculture. Cham: Springer; 2024. p. 89–126. https://doi.org/10.1007/978-3-031-52557-5_4
  14. 14. Ali WM, Abdel-Mageed MA, Hegazy MGA, Abou-Shlell MK, Sultan SME, Salama EAA, et al. Biocontrol agent of root-knot nematode Meloidogyne javanica and root-rot fungus Fusarium solani in okra: Morphological, anatomical characteristics and productivity under greenhouse conditions. Sci Rep. 2023;13:11103. https://doi.org/10.1038/s41598-023-37837-z
  15. 15. Ali Q, Khan AR, Tao S, Rajer FU, Ayaz M, Abro MA, et al. Broad-spectrum antagonistic potential of Bacillus spp. volatiles against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae. Physiol Plant. 2023;175:e14087. https://doi.org/10.1111/ppl.14087
  16. 16. Gowda MT, Rai AB, Singh B. Root-knot nematode: A threat to vegetable production and its management. Technical Bulletin No. 76. Varanasi: Indian Institute of Vegetable Research; 2017.
  17. 17. Davis EL, Hussey RS, Baum TJ. Parasitism proteins in nematode-plant interactions. Curr Opin Plant Biol. 2008;11:360–6. https://doi.org/10.1016/j.pbi.2008.04.003
  18. 18. Mohiddin FA, Khan MR. Root-knot nematode: Ignored soil-borne plant pathogen causing root diseases of chickpea. Eur J Biotechnol Biosci. 2014;2:4–10.
  19. 19. Dasgupta DR, Gaur HS. The root-knot nematodes Meloidogyne spp. in India. In: Swarup G, Dasgupta DR, editors. Plant parasitic nematodes of India: Problems and progress. New Delhi: Indian Agricultural Research Institute; 1986. p. 139–71.
  20. 20. Tyler J. Reproduction without males in aseptic root cultures of the root-knot nematode. Hilgardia. 1933;7:373–88. https://doi.org/10.3733/hilg.v07n10p373
  21. 21. Fullana AM, Expósito A, Escudero N, Cunquero M, Loza-Alvarez P, Giné A, et al. Crop rotation with Meloidogyne-resistant germplasm is useful to manage and revert the (a)virulent populations of Mi1.2 gene and reduce yield losses. Front Plant Sci. 2023;14:1133095. https://doi.org/10.3389/fpls.2023.1133095
  22. 22. Anushruti, Kumar S, Abha R, Sonkar VK, Mitra DS, Meena DK. Root-knot nematode management in vegetable crops: A review. Plant Arch. 2024;24:613–20.
  23. https://doi.org/10.51470/PLANTARCHIVES.2024.v24.no.2.086
  24. 23. Jhamta S, Thakur N, Ahluwalia KK. Management of root-knot nematode, Meloidogyne incognita in tomato through crop rotation and intercropping under polyhouse conditions. Indian Phytopathol. 2026;79:125–38. https://doi.org/10.1007/s42360-025-00928-0
  25. 24. Chandra B, Khan MR. Dynamics of soil nematodes in vegetable based crop sequences in West Bengal, India. J Plant Prot Res. 2011;51:7–13. https://doi.org/10.2478/v10045-011-0002-3
  26. 25. Umashankar KN, Krishnappa K, Reddy BMR, Ravichandra NG, Karuna K. Intercropping for the management of root-knot nematode, Meloidogyne incognita, in vegetable-based cropping systems. Indian J Nematol. 2005;35:46–9.
  27. 26. Din SU, Khan MA, Akram MT. Allelopathic potential of African marigold (Tagetes erecta) in sustainable tomato (Lycopersicon esculentum) production. J Appl Hortic. 2021;23:304–9. https://doi.org/10.37855/jah.2021.v23i03.54
  28. 27. Galbieri R, Kobayasti L, Albuquerque MCF, de Sá RO, Dutra SG, Boldt AS, et al. Castor bean as an option for Meloidogyne incognita management in cotton. Int J Pest Manag.2024;70:102–10. https://doi.org/10.1080/09670874.2021.1953633
  29. 28. Collange B, Navarrete M, Peyre G, Mateille T, Tchamitchian M. Root-knot nematode (Meloidogyne spp.) management in vegetable crop production: The challenge of an agronomic system analysis. Crop Prot. 2011;30:1251–62. https://doi.org/10.1016/j.cropro.2011.04.016
  30. 29. Khan MR, Jain RK, Ghule TM, Pal S. Root knot nematodes in India: A comprehensive monograph. New Delhi: All India Coordinated Research Project on Plant Parasitic Nematodes with Integrated Approach for their Control, Indian Agricultural Research Institute; 2014. 78 p.
  31. 30. Singh S. Integrated approach for the management of the root-knot nematode Meloidogyne incognita on eggplant under field conditions. Nematology. 2013;15:747–57.
  32. https://doi.org/10.1163/15685411-00002715
  33. 31. Jain RK, Gupta DC. Integrated management of root knot nematode (Meloidogyne javanica) infecting tomato and okra. Indian J Nematol. 1990;20:14–8.
  34. 32. Shutt VM, Affiah DU, Okechallu BO, Wonang DL. Efficacy of soil solarization on the control of root-knot nematodes infecting eggplant (Solanum melongena) in Plateau State. Afr J Plant Sci. 2021;15:144–50.
  35. 33. Jain RK. Tillage practices and soil solarization for nematode management. In: Gaur HS, Jain RK, Ganguly S, Sirohi A, Kamra A, editors. Nematode biodiversity, identification and role of agriculturally useful nematodes in soil health, dynamics of nematode community structure in major cropping systems and relevance in integrated nematode management. New Delhi: Division of Nematology, Indian Agricultural Research Institute; 2006. p. 230–6.
  36. 34. Bakr R, Mahdy M, Mousa M. Efficacy of soil solarization and post-planting mulch on control of root-knot nematodes. Pak J Nematol. 2013;31:71–6.
  37. 35. Rosskopf E, Di Gioia F, Hong JC, Pisani C, Kokalis-Burelle N. Organic amendments for pathogen and nematode control. Annu Rev Phytopathol. 2020;58:277–311. https://doi.org/10.1146/annurev-phyto-080516-035608
  38. 36. Singh S, Singh B, Singh AP. Integrated management of root-knot disease of okra caused by root-knot nematode Meloidogyne incognita. Indian J Nematol. 2014;44:172–8.
  39. 37. Patel S, Dhillon NK. Evaluation of sun hemp (Crotalaria juncea) as green manure/amendment and its biomass content on root-knot nematode (Meloidogyne incognita) in successive crop brinjal. J Entomol Zool Stud. 2017;5:716–20.
  40. 38. Ahmad F, Rather MA, Siddiqui MA. Nematicidal activity of leaf extracts from Lantana camara L. against Meloidogyne incognita (Kofoid and White) Chitwood and its use to manage root infection of Solanum melongena L. Braz Arch Biol Technol. 2010;53:543–8. https://doi.org/10.1590/S1516-89132010000300006
  41. 39. Asif M, Khan A, Tariq M, Siddiqui MA. Sustainable management of root-knot nematode Meloidogyne incognita through organic amendment on Solanum lycopersicum L. Asian J Biol. 2017;1:1–8. https://doi.org/10.9734/AJOB/2016/30739
  42. 40. McSorley R. Overview of organic amendments for management of plant-parasitic nematodes, with case studies from Florida. J Nematol. 2011;43:69–81.
  43. 41. Li J, La S, Song M, Gao L, Tian Y. Evidence for the role of soil C/N ratio in shaping plant responses to root-knot nematode infection. J Adv Res. 2026;79:51–60. https://doi.org/10.1016/j.jare.2025.03.036
  44. 42. United Nations Environment Programme. Handbook for the Montreal Protocol on substances that deplete the ozone layer: seventh edition. Nairobi: United Nations Environment Programme; 2006.
  45. 43. Ardakani AS. Toxicity of silver, titanium and silicon nanoparticles on the root-knot nematode, Meloidogyne incognita and growth parameters of tomato. Nematology. 2013;15:671–7. https://doi.org/10.1163/15685411-00002710
  46. 44. Siddiqui ZA, Khan A, Khan MR, Abd-Allah EF. Effects of zinc oxide nanoparticles and some plant pathogens on the growth and nodulation of lentil (Lens culinaris Medik.). Acta Phytopathol Entomol Hung. 2018;53:195–212. https://doi.org/10.1556/038.53.2018.012
  47. 45. Cromwell WA, Yang J, Starr JL, Jo YK. Nematicidal effects of silver nanoparticles on root-knot nematode in bermudagrass. J Nematol. 2014;46:261–6.
  48. 46. Baronia R, Kumar P, Singh SP, Walia RK. Silver nanoparticles as a potential nematicide against Meloidogyne graminicola. J Nematol. 2020;52:e2020-02. https://doi.org/10.21307/jofnem-2020-002
  49. 47. Thakur RK, Shirkot P. Potential of biogold nanoparticles to control plant pathogenic nematodes. J Bioanal Biomed. 2017;9:220–2.
  50. 48. Thakur RK, Dhirta B, Shirkot P. Studies on nanotoxicity effect of gold nanoparticles on Meloidogyne incognita and tomato plant growth and development. Ann Nanosci Nanotechnol. 2018;2:1005.
  51. 49. Dzięgielewska M, Skwiercz A, Wesołowska A, Kozacki D, Przewodowski W, Kulpa D. Effects of silver, gold and platinum nanoparticles on selected nematode trophic groups. J Hortic Res. 2023;31:23–34. https://doi.org/10.2478/johr-2023-0035
  52. 50. Williamson VM. Root-knot resistance genes in tomato and their potential for future use. Annu Rev Phytopathol. 1998;36:277–93. https://doi.org/10.1146/annurev.phyto.36.1.277
  53. 51. Milligan SB, Bodeau J, Yaghoobi J, Kaloshian I, Zabel P, Williamson VM. The root-knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes. Plant Cell. 1998;10:1307–19. https://doi.org/10.1105/tpc.10.8.1307
  54. 52. Medina-Filho HP, Stevens MA. Tomato breeding for nematode resistance: survey of resistant varieties for horticultural characteristics and genotype of acid phosphatase. Acta Hortic. 1980;100:383–91. https://doi.org/10.17660/ActaHortic.1980.100.41
  55. 53. Ammiraju JS, Veremis JC, Huang X, Roberts PA, Kaloshian I. The heat-stable root-knot nematode resistance gene Mi-9 from Lycopersicon peruvianum is localized on the short arm of chromosome 6. Theor Appl Genet. 2003;106:478–84. https://doi.org/10.1007/s00122-002-1106-y
  56. 54. Jablonska B, Ammiraju JSS, Bhattarai KK, Mantelin S, de Ilarduya OM, Roberts PA, et al. The Mi-9 gene from Solanum arcanum conferring heat-stable resistance to root-knot nematodes is a homolog of Mi-1. Plant Physiol. 2007;143:1044–54. https://doi.org/10.1104/pp.106.089615
  57. 55. Patel VS, Pitambara, Shukla YM. Screening of various tomato genotypes for resistance to root-knot nematode (Meloidogyne incognita) infection. Int J Chem Stud. 2018;6:1434–8.
  58. 56. Maurya D, Mukherjee A, Bhagyashree, Sangam S, Kumar R, Akhtar S, et al. Marker assisted stacking of Ty3, Mi1.2 and Ph3 resistance alleles for leaf curl, root knot and late blight diseases in tomato. Physiol Mol Biol Plants. 2023;29:121–9. https://doi.org/10.1007/s12298-022-01277-2
  59. 57. Williamson V, Ho J, Wu F, Miller N, Kaloshian I. A PCR-based marker tightly linked to the nematode resistance gene Mi in tomato. Theor Appl Genet. 1994;87:757–63.
  60. https://doi.org/10.1007/BF00221126
  61. 58. Zhou X, Liu J, Bao S, Yang Y, Zhuang Y. Molecular cloning and characterization of a wild eggplant Solanum aculeatissimum NBS-LRR gene involved in plant resistance to Meloidogyne incognita. Int J Mol Sci. 2018;19:583. https://doi.org/10.3390/ijms19020583
  62. 59. Hepher A, Atkinson HJ. Nematode control with proteinase inhibitors. European Patent Application No. 92301890.7; Publication No. 0 502 730 A1. 1992.
  63. 60. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998;391:806–11. https://doi.org/10.1038/35888
  64. 61. Yadav BC, Veluthambi K, Subramaniam K. Host-generated double-stranded RNA induces RNAi in plant parasitic nematodes and protects the host from infection. Mol Biochem Parasitol. 2006;148:219–22.https://doi.org/10.1016/j.molbiopara.2006.03.013
  65. 62. Koulagi R, Sirohi A. Gene stacking through Agrobacterium-mediated co-transformation in tomato to engineer resistance against root-knot nematode Meloidogyne incognita and tomato leaf curl virus. Indian J Nematol. 2015;45:161–8.
  66. 63. Banerjee S, Gill SS, Gawade BH, Jain PK, Subramaniam K, Sirohi A. Host-delivered RNAi of two cuticle collagen genes, Mi-col-1 and Lemmi-5, hampers structure and fecundity in Meloidogyne incognita. Front Plant Sci. 2018;8:2266. https://doi.org/10.3389/fpls.2017.02266
  67. 64. Liang Z, Ali Q, Wang Y, Mu G, Kan X, Ren Y, et al. Toxicity of Bacillus thuringiensis strains derived from the novel crystal protein Cry31Aa with high nematicidal activity against rice parasitic nematode Aphelenchoides besseyi. Int J Mol Sci. 2022;23:8189. https://doi.org/10.3390/ijms23158189
  68. 65. Rao MS, Kamalnath M, Umamaheshwari R, Rajanikanth R, Prabu P, Priti K, et al. Bacillus subtilis IIHR BS-2 enriched vermicompost controls root-knot nematode and soft rot disease complex in carrot. Sci Hortic. 2017;218:56–62. https://doi.org/10.1016/j.scienta.2017.01.051
  69. 66. Mostafa El-Ansary MS, Hegazy WK, Hamouda RA. Positive regulation of root-knot nematode control in Capsicum through bioactive compounds derived from plant seed cake. Sci Rep. 2025;15:20325. https://doi.org/10.1038/s41598-025-06467-y
  70. 67. Vinothini K, Nakkeeran S, Saranya N, Jothi P, Richard JI, Perveen K, et al. Rhizosphere engineering of biocontrol agents enriches soil microbial diversity and effectively controls root-knot nematodes. Microb Ecol. 2024;87:120. https://doi.org/10.1007/s00248-024-02435-7
  71. 68. Ayaz M, Li CH, Ali Q, Zhao W, Chi YK, Shafiq M, et al. Bacterial and fungal biocontrol agents for plant disease protection: Journey from lab to field, current status, challenges and global perspectives. Molecules. 2023;28:6735. https://doi.org/10.3390/molecules28186735
  72. 69. Swarnakumari N. Role of bacterial bioagent Pasteuria penetrans in the management of root-knot nematode Meloidogyne incognita by altering the lifecycle. Pest Manag Hortic Ecosyst. 2017;23:76–80.
  73. 70. Orr JN, Neilson R, Freitag TE, Roberts DM, Davies KG, Blok VC, et al. Parallel microbial ecology of Pasteuria and nematode species in Scottish soils. Front Plant Sci. 2020;10:1763. https://doi.org/10.3389/fpls.2019.01763
  74. 71. Vagelas I, Dennett MD, Pembroke B, Gowen SR. Adhering Pasteuria penetrans endospores affect movements of root-knot nematode juveniles. Phytopathol Mediterr. 2012;51:618–24.
  75. 72. Aioub AAA, Elesawy AE, Ammar EE. Plant growth promoting rhizobacteria (PGPR) and their role in plant-parasitic nematodes control: A fresh look at an old issue. J Plant Dis Prot. 2022;129:1305–21. https://doi.org/10.1007/s41348-022-00642-3
  76. 73. Li X, Hu HJ, Li JY, Wang C, Chen SL, Yan SZ. Effects of the endophytic bacteria Bacillus cereus BCM2 on tomato root exudates and Meloidogyne incognita infection. Plant Dis. 2019;103:1551–8. https://doi.org/10.1094/PDIS-11-18-2016-RE
  77. 74. Yu Z, Xiong J, Zhou Q, Luo H, Hu S, Xia L, et al. The diverse nematicidal properties and biocontrol efficacy of Bacillus thuringiensis Cry6A against the root-knot nematode Meloidogyne hapla. J Invertebr Pathol. 2015;125:73–80. https://doi.org/10.1016/j.jip.2014.12.011
  78. 75. Khan A, Haris M, Hussain T, Khan AA, Laasli SE, Lahlali R, et al. Counter-attack of biocontrol agents: Environmentally benign approaches against root-knot nematodes (Meloidogyne spp.) on agricultural crops. Heliyon. 2023;9:e21653. https://doi.org/10.1016/j.heliyon.2023.e21653
  79. 76. Vasantha-Srinivasan P, Park KB, Kim KY, Jung WJ, Han YS. The role of Bacillus species in the management of plant-parasitic nematodes. Front Microbiol. 2025;15:1510036. https://doi.org/10.3389/fmicb.2024.1510036
  80. 77. Cimen H, Touray M, Gulsen SH, Hazir S. Natural products from Photorhabdus and Xenorhabdus: Mechanisms and impacts. Appl Microbiol Biotechnol. 2022;106:4387–99. https://doi.org/10.1007/s00253-022-12023-9
  81. 78. Kang BR, Anderson AJ, Kim YC. Hydrogen cyanide produced by Pseudomonas chlororaphis O6 exhibits nematicidal activity against Meloidogyne hapla. Plant Pathol J.2018;34:35–43. https://doi.org/10.5423/PPJ.OA.06.2017.0115
  82. 79. Chen W, Zhu Z, Liu C, Yang F, Dai W, Yu H, et al. Evaluation of Virgibacillus dokdonensis MCCC 1A00493 as a biological control agent and microbial organic fertilizer against root-knot nematodes. Biol Control. 2024;192:105508. https://doi.org/10.1016/j.biocontrol.2024.105508
  83. 80. Xia Y, Xie S, Ma X, Wu H, Wang X, Gao X. The purL gene of Bacillus subtilis is associated with nematicidal activity. FEMS Microbiol Lett. 2011;322:99–107.
  84. https://doi.org/10.1111/j.1574-6968.2011.02336.x
  85. 81. Zhang J, Li Y, Yuan H, Sun B, Li H. Biological control of the cereal cyst nematode (Heterodera filipjevi) by Achromobacter xylosoxidans isolate 09X01 and Bacillus cereus isolate 09B18. Biol Control. 2016;92:1–6. https://doi.org/10.1016/j.biocontrol.2015.08.004
  86. 82. Proença DN, Schwab S, Vidal MS, Baldani JI, Xavier GR, Morais PV. The nematicide Serratia plymuthica M24T3 colonizes Arabidopsis thaliana, stimulates plant growth and presents plant beneficial potential. Braz J Microbiol. 2019;50:777–89. https://doi.org/10.1007/s42770-019-00098-y
  87. 83. Zhao D, Zhao H, Zhao D, Zhu X, Wang Y, Duan Y, et al. Isolation and identification of bacteria from rhizosphere soil and their effect on plant growth promotion and root-knot nematode disease. Biol Control. 2018;119:12–19. https://doi.org/10.1016/j.biocontrol.2018.01.004
  88. 84. Shi J, Sun M. Bacillus thuringiensis: A gift for nematode management. Trends Parasitol. 2025;41:235–46. https://doi.org/10.1016/j.pt.2025.01.004
  89. 85. Khalil MS, Kenawy A, Gohrab MA, Mohammed EE. Impact of microbial agents on Meloidogyne incognita management and morphogenesis of tomato. J Biopestic. 2012;5:28–35. https://doi.org/10.57182/jbiopestic.5.1.28-35
  90. 86. Almaghrabi OA, Massoud SI, Abdelmoneim TS. Influence of inoculation with plant growth-promoting rhizobacteria on tomato plant growth and nematode reproduction under greenhouse conditions. Saudi J Biol Sci. 2013;20:57–61. https://doi.org/10.1016/j.sjbs.2012.10.004
  91. 87. Sharma IP, Sharma AK. Effective control of root-knot nematode disease with Pseudomonad rhizobacteria filtrate. Rhizosphere.2017;3:123–5. https://doi.org/10.1016/j.rhisph.2017.02.001
  92. 88. Zhao J, Wang S, Zhu X, Wang Y, Liu X, Duan Y, et al. Isolation and characterization of nodule endophytic bacteria Pseudomonas protegens Sneb1997 and Serratia plymuthica Sneb2001 for the biological control of root-knot nematode. Appl Soil Ecol. 2021;164:103924. https://doi.org/10.1016/j.apsoil.2021.103924
  93. 89. Antil S, Kumar R, Pathak D, Kumar A, Panwar A, Kumari A. Plant growth-promoting rhizobacteria Bacillus cereus KMT-5 and Bacillus megaterium KMT-8 effectively suppressed Meloidogyne javanica infection. Appl Soil Ecol. 2022;174:104419. https://doi.org/10.1016/j.apsoil.2022.104419
  94. 90. Devindrappa M, Kamra A, Singh D, Gawade B, Sirohi A. Plant growth promoting Bacillus species elicit defense against Meloidogyne incognita infecting tomato in polyhouse. J Basic Microbiol. 2023;63:1233–41. https://doi.org/10.1002/jobm.202300146
  95. 91. Gowda MT, Reddy YS, Patil J, Krishna H, Sellaperumal C, Manjunath M, et al. Biocontrol potential of Bacillus subtilis CRB7 for the management of root-knot nematode in tomato under protected cultivation. Arch Phytopathol Plant Prot. 2024;57:800–22. https://doi.org/10.1080/03235408.2024.2414474
  96. 92. El-Nagdi WMA, Abd-El-Khair H. Application of Bacillus species for controlling root-knot nematode Meloidogyne incognita in eggplant. Bull Natl Res Cent. 2019;43:154. https://doi.org/10.1186/s42269-019-0187-6
  97. 93. Acharya A, Sahu A, Das BK. Effect of bacterial antagonists on root-knot nematode Meloidogyne incognita infecting brinjal. Int J Curr Microbiol Appl Sci. 2020;9:171–7.https://doi.org/10.20546/ijcmas.2020.903.021
  98. 94. Das S, Wadud MA, Chakraborty S, Khokon MAR. Biorational management of root-knot of brinjal (Solanum melongena L.) caused by Meloidogyne javanica. Heliyon.2022;8:e09227.https://doi.org/10.1016/j.heliyon.2022.e09227
  99. 95. Yin N, Zhao JL, Liu R, Li Y, Ling J, Yang YH, et al. Biocontrol efficacy of Bacillus cereus strain Bc-cm103 against Meloidogyne incognita. Plant Dis. 2021;105:2061–70. https://doi.org/10.1094/PDIS-03-20-0648-RE
  100. 96. Dai MM, Liu R, Jiang H, Zhang XP, Song WW, Zhang J, et al. Volatile organic compounds of Bacillus pumilus strain S1-10 exhibit fumigant activity against Meloidogyne incognita. Plant Dis. 2023;107:3057–63. https://doi.org/10.1094/PDIS-10-22-2391-RE
  101. 97. Rahman MU, Zhong X, Uzair M, Fan B. Application of fungi as biological control strategies for nematode management in horticultural crops. Phytopathol Res. 2024;6:38.https://doi.org/10.1186/s42483-024-00257-6
  102. 98. Zhu MC, Li XM, Zhao N, Yang L, Zhang KQ, Yang JK. Regulatory mechanism of trap formation in the nematode-trapping fungi. J Fungi (Basel). 2022;8:406. https://doi.org/10.3390/jof8040406
  103. 99. Thakur NSA, Devi G. Management of Meloidogyne incognita attacking okra by nematophagous fungi Arthrobotrys oligospora and Paecilomyces lilacinus. Agric Sci Dig. 2007;27:50–2.
  104. 100. Khan MR, Goswami BK. Cladosporium oxysporum, an egg parasitic fungus of Meloidogyne incognita. Indian J Nematol. 2001;32:169–70.
  105. 101. Khan M, Tanaka K. Purpureocillium lilacinum for plant growth promotion and biocontrol against root-knot nematodes infecting eggplant. PLoS One. 2023;18:e0283550. https://doi.org/10.1371/journal.pone.0283550
  106. 102. Yao Y, Huo J, Ben H, Gao W, Hao Y, Wang W, et al. Biocontrol efficacy of endophytic fungus Acremonium sclerotigenum against Meloidogyne incognita under in vitro and in vivo conditions. Biologia. 2023;78:3305–13. https://doi.org/10.1007/s11756-023-01505-4
  107. 103. Tolba SRT, Moustafa MMA, Elshawaf IIS, Rosso LC, Pentimone I, Colagiero M, et al. Root endophytism by Pochonia chlamydosporia affects defense-gene expression in leaves of monocot and dicot hosts under multiple biotic interactions. Plants (Basel). 2021;10:718. https://doi.org/10.3390/plants10040718
  108. 104. Tyśkiewicz R, Nowak A, Ozimek E, Jaroszuk-Ściseł J. Trichoderma: the current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. Int J Mol Sci. 2022;23:2329. https://doi.org/10.3390/ijms23042329
  109. 105. Haseeb A, Khan MR. Prospects of Trichoderma in nematode management. In: Nagesh M, Rajkumar BS, Bhumannavar NK, Krishna Kumar, editors. Status and prospects for enhancing the uptake of antagonistic organisms for nematode management in India. Bangalore: NBAII (ICAR); 2012. p. 187.
  110. 106. Yan Y, Mao Q, Wang Y, Zhao J, Fu Y, Yang Z, et al. Trichoderma harzianum induces resistance to root-knot nematodes by increasing secondary metabolite synthesis and defense-related enzyme activity in Solanum lycopersicum. Biol Control. 2021;158:104609. https://doi.org/10.1016/j.biocontrol.2021.104609
  111. 107. Dababat AAF, Sikora R. Use of Trichoderma harzianum and Trichoderma viride for biological control of Meloidogyne incognita on tomato. Jordan J Agric Sci. 2007;3:953–61
  112. 108. Ghahremani Z, Escudero N, Saus E, Gabaldón T, Sorribas FJ. Pochonia chlamydosporia induces plant-dependent systemic resistance to Meloidogyne incognita. Front Plant Sci. 2019;10:945. https://doi.org/10.3389/fpls.2019.00945
  113. 109. Singh S, Mathur N. Biological control of root-knot nematode (Meloidogyne incognita) infesting tomato. Biocontrol Sci Technol.2010;20:865–74. https://doi.org/10.1080/09583157.2010.487935
  114. 110. Yao YR, Tian XL, Shen BM, Mao ZC, Chen GH, Xie BY. Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita. World J Microbiol Biotechnol. 2015;31:549–56.https://doi.org/10.1007/s11274-014-1781-2
  115. 111. Hussain M, Zouhar M, Rysanek P. Population dynamics of a nematophagous fungus Lecanicillium muscarium and root-knot nematode (Meloidogyne incognita) to assess the disease pressure and its management. Pak J Zool. 2017;49:197–204. https://doi.org/10.17582/journal.pjz/2017.49.1.197.204
  116. 112. Ahmed S, Monjil MS. Effect of Paecilomyces lilacinus on tomato plants and the management of root-knot nematodes. J Bangladesh Agric Univ. 2019;17:9–13. https://doi.org/10.3329/jbau.v17i1.40657
  117. 113. He Q, Wang D, Li B, Maqsood A, Wu H. Nematicidal evaluation and active compounds isolation of Aspergillus japonicus ZW1 against root-knot nematodes (Meloidogyne incognita). Agronomy. 2020;10:1222. https://doi.org/10.3390/agronomy10091222
  118. 114. Soliman MS, El-Deriny MM, Ibrahim DSS, Zakaria H, Ahmed Y. Suppression of root-knot nematode Meloidogyne incognita on tomato plants using the nematode-trapping fungus Arthrobotrys oligospora. J Appl Microbiol. 2021;131:2402–15. https://doi.org/10.1111/jam.15101
  119. 115. Rostami M, Shahbazi S, Soleimani R, Ghorbani A. Optimizing sustainable control of Meloidogyne javanica in tomato plants through gamma radiation-induced mutants of Trichoderma harzianum and Bacillus velezensis. Sci Rep. 2024;14:17774. https://doi.org/10.1038/s41598-024-68365-z
  120. 116. Jeevan H, Patidar RK, Kadam V, Dutta P, Nongbri E, Gouda MNR, et al. Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya. Sci Rep. 2025;15:602. https://doi.org/10.1038/s41598-024-84292-5
  121. 117. Uddin J, Ullah F, Naz I, Ahmad S, Saljoqi AUR, Khan SS et al. Root-knot nematode pathogen suppression in eggplant using antagonistic fungi. Egypt J Biol Pest Control. 2023;33:15. https://doi.org/10.1186/s41938-023-00659-2
  122. 118. Parveen A, Tanaka K, Khan M. Biocontrol efficacy of Pochonia chlamydosporia against root-knot nematode Meloidogyne javanica in eggplant and its impact on plant growth. Sci Rep. 2025;15:36990. https://doi.org/10.1038/s41598-025-99527-2
  123. 119. Mohammed RKA, Khan MR. Management of root-knot nematode in cucumber through seed treatment with multifarious beneficial microbes in polyhouse under protected cultivation. Indian Phytopathol. 2021;74:1035–43. https://doi.org/10.1007/s42360-021-00422-3
  124. 120. Singh AK, Kranti KKVVS, Paschapur AU, Begum S, Bhatt S, Gouri V, et al. Comparative evaluation of biological and chemical treatments for root-knot nematode management in cucumber under Mid-Himalayan protected cultivation. Discov Sustain. 2026;7:260. https://doi.org/10.1007/s43621-025-01950-y
  125. 121. Pofu KM, Mashela PW. Interactive effects of filamentous fungi and cucurbitacin phytonematicide on growth of cowpea and suppression of Meloidogyne enterolobii. Front Microbiol.2022;13:765051. https://doi.org/10.3389/fmicb.2022.765051
  126. 122. Bawa N, Kaur S, Dhillon NK. Integrated management of root-knot nematode (Meloidogyne incognita) in capsicum using Paecilomyces lilacinus and organic amendments. J Entomol Zool Stud. 2020;8:1693–701.
  127. 123. Li L, Sun Y, Chen F, Hao D, Tan J. An alkaline protease from Bacillus cereus NJSZ-13 can act as a pathogenicity factor in infection of pinewood nematode. BMC Microbiol. 2023;23:10.https://doi.org/10.1186/s12866-022-02752-2
  128. 124. Castaneda-Alvarez C, Prodan S, Rosales IM, Aballay E. Exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on Xiphinema index Thorne & Allen. J Appl Microbiol. 2016;120:413–24. https://doi.org/10.1111/jam.12987
  129. 125. Ahmed N, Ghramh HA, Shakeel Q, Ashraf W, Abbas HT, Binyamin R, et al. Evaluation of rhizospheric-Pseudomonas spp. for the management of Meloidogyne incognita in tomato. J King Saud Univ Sci. 2023;35:102395. https://doi.org/10.1016/j.jksus.2022.102395
  130. 126. Shahid M, Shafi Z. Multi-omics and molecular engineering insights into Pseudomonas spp.-mediated biocontrol of root-knot nematodes: an updated perspective. Physiol Mol Plant Pathol.2025;140:102972. https://doi.org/10.1016/j.pmpp.2025.102972
  131. 127. Gao H, Qi G, Yin R, Zhang H, Li C, Zhao X. Bacillus cereus strain S2 shows high nematicidal activity against Meloidogyne incognita by producing sphingosine. Sci Rep. 2016;6:28756.https://doi.org/10.1038/srep28756
  132. 128. Du C, Cao S, Shi X, Nie X, Zheng J, Deng Y, et al. Genetic and biochemical characterization of a gene operon for trans-aconitic acid, a novel nematicide from Bacillus thuringiensis. J Biol Chem. 2017;292:3517–30. https://doi.org/10.1074/jbc.M116.762666
  133. 129. Seong J, Shin J, Kim K, Cho BK. Microbial production of nematicidal agents for controlling plant-parasitic nematodes. Process Biochem. 2021;108:69–79. https://doi.org/10.1016/j.procbio.2021.06.006
  134. 130. Deng X, Wang X, Li G. Nematicidal effects of volatile organic compounds from microorganisms and plants on plant-parasitic nematodes. Microorganisms. 2022;10:1201. https://doi.org/10.3390/microorganisms10061201
  135. 131. Wolstenholme AJ, Rogers AT. Glutamate-gated chloride channels and the mode of action of the avermectin/milbemycin anthelmintics. Parasitology. 2005;131:S85–95. https://doi.org/10.1017/S0031182005008218
  136. 132. El-Ashry RM, Ali MAS, Elsobki AEA, Aioub AAA. Integrated management of Meloidogyne incognita on tomato using combinations of abamectin, Purpureocillium lilacinum, rhizobacteria and botanicals compared with nematicide. Egypt J Biol Pest Control. 2021;31:93. https://doi.org/10.1186/s41938-021-00438-x
  137. 133. Pereira CG, Varejão JOS, Barros AF, Paiva WF, Balbino HM, Rodrigues D, et al. Identification and fumigant effects of nematicidal volatile organic compounds produced by nematophagous fungi against root-knot nematodes. BioControl.2026;71:613–25. https://doi.org/10.1007/s10526-026-10384-y
  138. 134. Wang Y, Li L, Li D, Wang B, Zhang K, Niu X. Yellow pigment aurovertins mediate interactions between the pathogenic fungus Pochonia chlamydosporia and its nematode host. J Agric Food Chem. 2015;63:6577–87. https://doi.org/10.1021/acs.jafc.5b02595
  139. 135. Abbas A, Ali S, Mubeen M, Hussain A, Gutumsary KA, Hussain B, et al. Talaromyces spp. are promising biocontrol agents for sustainable agriculture. In: Kumar A, Solanki MK, editors. Microbial biocontrol techniques. Microorganisms for Sustainability. Vol. 54. Singapore: Springer; 2024. p. 245–80. https://doi.org/10.1007/978-981-97-8739-5_13
  140. 136. Youssef MMA, El-Nagdi WMA, Lotfy DEM. Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode Meloidogyne incognita on cowpea. Bull Natl Res Cent. 2020;44:1–11. https://doi.org/10.1186/s42269-020-00367-z
  141. 137. Siddiqui ZA, Azi S. Plant parasitic nematode-fungus interactions: recent concepts and mechanisms. Plant Physiol Rep. 2024;29:37–50. https://doi.org/10.1007/s40502-023-00762-4
  142. 138. Cai S, Jia J, He C, Zeng L, Fang Y, Qiu G, et al. Multi-omics of pine wood nematode pathogenicity associated with culturable associated microbiota through an artificial assembly approach. Front Plant Sci. 2022;12:798539. https://doi.org/10.3389/fpls.2021.798539
  143. 139. Sharma N, Khanna K, Kaur R, Jasrotia S, Parihar RD, Khajuria A, et al. Soil microbiota and mechanisms of plant parasitic nematode suppression. In: Chaudhary KK, Meghvansi MK, Siddiqui S, editors. Sustainable management of nematodes in agriculture. Vol. 2. Role of microbes-assisted strategies. Sustainability in Plant and Crop Protection. Vol. 19. Cham: Springer; 2024. p. 49–87. https://doi.org/10.1007/978-3-031-52557-5_3
  144. 140. Lahlali R, Ibrahim DSS, Belabess Z, Roni MZK, Radouane N, Vicente CSL, et al. High-throughput molecular technologies for unraveling the mystery of soil microbial community: challenges and future prospects. Heliyon. 2021;7:e08142. https://doi.org/10.1016/j.heliyon.2021.e08142
  145. 141. Nafea AM, Wang Y, Wang D, Salama AM, Aziz MA, Xu S, et al. Application of next-generation sequencing to identify different pathogens. Front Microbiol. 2024;14:1329330.
  146. https://doi.org/10.3389/fmicb.2023.1329330
  147. 142. Singh A, Joshi I, Kumar A, Dinkar V, Kohli D, Koulagi R, et al. Harnessing nematode-responsive promoters: a promising solution for plant parasitic nematodes management. Plant Stress. 2025;16:100835. https://doi.org/10.1016/j.stress.2025.100835
  148. 143. Crandall SG, Gold KM, Jiménez-Gasco MM, Filgueiras CC, Willett DS. A multi-omics approach to solving problems in plant disease ecology. PLoS One. 2020;15:e0237975. https://doi.org/10.1371/journal.pone.0237975
  149. 144. Yi X, Guo Y, Khan RAA, Fan Z. Understanding the pathogenicity of Pochonia chlamydosporia to root-knot nematode through omics approaches and action mechanism. Biol Control. 2021;162:104726. https://doi.org/10.1016/j.biocontrol.2021.104726
  150. 145. Meel S, Saharan BS. Microbial warfare against nematodes: a review of nematicidal compounds for horticulture, environment and biotechnology. Microbe. 2025;9:100557. https://doi.org/10.1016/j.microb.2025.100557
  151. 146. Nakayasu ES, Nicora CD, Sims AC, Burnum-Johnson KE, Kim YM, Kyle JE, et al. MPLEx: a robust and universal protocol for single-sample integrative proteomic, metabolomic and lipidomic analyses. mSystems. 2016;1:e00043-16. https://doi.org/10.1128/mSystems.00043-16
  152. 147. Topalović O, Hussain M, Heuer H. Plants and associated soil microbiota cooperatively suppress plant-parasitic nematodes. Front Microbiol. 2020;11:313. https://doi.org/10.3389/fmicb.2020.00313
  153. 148. Desmedt W, Mangelinckx S, Kyndt T, Vanholme B. A phytochemical perspective on plant defense against nematodes. Front Plant Sci. 2020;11:602079. https://doi.org/10.3389/fpls.2020.602079
  154. 149. Gebauer J, Pietruszka J, Classen T. Expression and characterization of PrnC-a flavin-dependent halogenase from the pyrrolnitrin biosynthetic pathway of Pseudomonas protegens Pf-5. Front Catal. 2023;3:1231765. https://doi.org/10.3389/fctls.2023.1231765
  155. 150. Wang S, Wang Y, Yao Y, Li W, Hu Z, Li D, et al. Integrated management of bacterial wilt and root-knot nematode diseases in pepper: discovery of phenazine-1-carboxamide from Pseudomonas aeruginosa W-126. Int J Mol Sci. 2025;26:3335. https://doi.org/10.3390/ijms26073335

Downloads

Download data is not yet available.