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Plant Science Today (PST)

Research Articles

Early Access

Evaluation and screening of rice (Oryza sativa L.) genotypes for drought tolerance under reproductive-stage stress

DOI
https://doi.org/10.14719/pst.15675
Submitted
23 May 2026
Published
23-09-2026
Versions

Abstract

Drought stress at the reproductive-stage is a major constraint limiting rice productivity in rainfed ecosystems. The present study evaluated 450 rice (Oryza sativa L.) genotypes along with 8 checks under normal and reproductive-stage drought stress conditions during kharif 2020 at Bihar Agricultural University, Sabour, India, using an augmented randomised complete block design. Drought stress was imposed by withholding irrigation 25–30 days before flowering. Significant variation was observed among genotypes for all studied traits under both environments, indicating substantial genetic diversity for drought response. Stress conditions caused marked reductions in grain yield and related traits, particularly panicle fertility and filled grains per panicle, due to increased spikelet sterility. Genotypes were initially screened using drought susceptibility index (DSI) and those with a DSI of less than 1 were shortlisted for further analysis. To ensure stringent selection, genotypes were ranked based on DSI values and the lowest 20th percentile was considered as drought-tolerant. Further evaluation based on yield under stress and key reproductive traits identified 26 promising drought-tolerant genotypes. Among these, six genotypes, namely ARC 11245 (IRGC 42651-2), SAITA (IRGC 31618-1), KWANG LU AI 4 (IRGC 28480-2), DM 49 (IRGC 8775-1), CRILLO LA FRIA (IRGC 10793-1) and 77 UPLA (GERVEX 159-C1), exhibited DSI values of zero and showed superior yield stability under stress. Principal component analysis revealed that the first three components explained 79.68 % of the total variation, with grain yield, filled grains, total grains and panicle fertility contributing most to variability under stress. The identified genotypes provide promising genetic resources for future drought-tolerance breeding following multi-environment validation.

References

  1. 1. Food and Agriculture Organization of the United Nations. FAOSTAT statistical database. Rome: FAO; 2023.
  2. 2. Gouda G, Gupta MK, Donde R, Mohapatra T, Vadde R, Behera L. Marker-assisted selection for grain number and yield-related traits of rice (Oryza sativa L.). Physiol Mol Biol Plants. 2020;26:885–98. https://doi.org/10.1007/s12298-020-00773-7
  3. 3. Khush GS. Origin, dispersal, cultivation and variation of rice. Plant Mol Biol. 1997;35(1–2):25–34. https://doi.org/10.1023/A:1005810616885
  4. 4. Indian Council of Agricultural Research. Vision 2050: Indian agriculture towards self-reliance. New Delhi: ICAR; 2021.
  5. 5. Pantuwan G, Fukai S, Cooper M, Rajatasereekul S, O’Toole JC. Yield response of rice (Oryza sativa L.) genotypes to different types of drought under rainfed lowlands: Part 1. Grain yield and yield components. Field Crops Res. 2002;73:153–68. https://doi.org/10.1016/S0378-4290(01)00187-3
  6. 6. Alam Khan M. Current status of genomic-based approaches to enhance drought tolerance in rice (Oryza sativa L.): An overview. Mol Plant Breed. 2012;3:1–10. https://doi.org/10.5376/MPB.2012.03.0001
  7. 7. Xangsayasane P, Jongdee B, Pantuwan G, Fukai S, Mitchell JH, Inthapanya P. Genotypic performance under intermittent and terminal drought screening in rainfed lowland rice. Field Crops Res. 2014;156:281–92. https://doi.org/10.1016/j.fcr.2013.10.017
  8. 8. Kumar V, Ladha JK. Direct seeding of rice: Recent developments and future research needs. Adv Agron. 2011;111:297–413. https://doi.org/10.1016/B978-0-12-387689-8.00001-1
  9. 9. International Rice Research Institute. Rice breeding innovations for drought-prone environments. Los Baños: IRRI; 2018.
  10. 10. Bernier J, Kumar A, Venuprasad R, Spaner D, Atlin GN. A large-effect QTL for grain yield under reproductive-stage drought stress in upland rice. Crop Sci. 2007;47(2):507–16. https://doi.org/10.2135/cropsci2006.07.0495
  11. 11. Vikram P, Swamy BPM, Dixit S, Ahmed HU, Cruz MTS, Singh AK, et al. qDTY1.1, a major QTL for rice grain yield under reproductive-stage drought stress with a consistent effect in multiple elite genetic backgrounds. BMC Genet. 2011;12:89. https://doi.org/10.1186/1471-2156-12-89
  12. 12. Fischer RA, Maurer R. Drought resistance in spring wheat cultivars. I. Grain yield responses. Aust J Agric Res. 1978;29(5):897–912. https://doi.org/10.1071/AR9780897
  13. 13. Mall AK, Swain P, Das S, Singh ON, Kumar A. Effect of drought on yield and drought susceptibility index for quality characters of promising rice genotypes. Cereal Res Commun. 2011;39(1):22–31. https://doi.org/10.1556/CRC.39.2011.1.3
  14. 14. Tarang A, Haghighi Hasanalideh A. Quantitative indices for drought tolerance in rice: Leveraging genetic resources for climate-resilient breeding. Plant Environ Interact. 2026;7(3):e70145. https://doi.org/10.1002/pei3.70145
  15. 15. Parray SA, Ramalingam AP, Tugoo MZ, Serba DD, Vara Prasad PV, Perumal R. Harnessing genetic diversity for drought tolerance and forage yield improvement in pearl millet germplasms through a prebreeding framework. Sci Rep. 2025;15:40033. https://doi.org/10.1038/s41598-025-23884-1
  16. 16. Prasad B, Patil SS, Jambhale ND. Genetic divergence in rice. Oryza. 2013;50(4):317–22.
  17. 17. Nachimuthu VV, Muthurajan R, Duraialaguraja S, Sivakami R, Pandian BA, Ponniah G, et al. Analysis of population structure and genetic diversity in rice germplasm using SSR markers. Plant Mol Biol Rep. 2015;33:1–13.
  18. 18. Singh S, Prasad S, Yadav V, Kumar A, Jaiswal B, Kumar A, et al. Effect of drought stress on yield and yield components of rice (Oryza sativa L.) genotypes. Int J Curr Microbiol Appl Sci. 2018;7:2752–9.
  19. 19. Yang X, Wang B, Chen L, Li P, Cao C. The different influences of drought stress at the flowering stage on rice physiological traits, grain yield and quality. Sci Rep. 2019;9:3742. https://doi.org/10.1038/s41598-019-40161-0
  20. 20. Swain P, Raman A, Singh SP, Kumar A. Breeding drought tolerant rice for shallow rainfed ecosystem of eastern India. Field Crops Res. 2017;209:168–78. https://doi.org/10.1016/j.fcr.2017.05.007
  21. 21. Garg P, Dey A, Roy B, Mishra D. Genetic diversity analysis of rice under drought and irrigated conditions. J Pharmacogn Phytochem. 2017;6(6):2449–54.
  22. 22. Anjum SA, Ashraf U, Tanveer M, Khan I, Hussain S, Shahzad B, et al. Drought-induced changes in growth, osmolyte accumulation and antioxidant metabolism of three maize hybrids. Front Plant Sci. 2017;8:69. https://doi.org/10.3389/fpls.2017.00069
  23. 23. Hsiao TC, Fereres E, Acevedo E, Henderson DW. Water stress and dynamics of growth and yield of crop plants. In: Water and Plant Life. 1976. p. 281–305. https://doi.org/10.1007/978-3-642-66429-8_18
  24. 24. Kumar R, Sarawgi AK, Ramos C, Amarante ST, Ismail AM, Wade LJ. Partitioning of dry matter during drought stress in rainfed lowland rice. Field Crops Res. 2006;96:455–65. https://doi.org/10.1016/j.fcr.2005.09.015
  25. 25. Davatgar N, Neishabouri MR, Sepaskhah AR, Soltani A. Physiological and morphological responses of rice (Oryza sativa L.) to varying water stress management strategies. Int J Plant Prod. 2009;3:1735–8043. https://doi.org/10.22069/IJPP.2012.660
  26. 26. Zou G, Mei H, Liu H, Liu G, Hu S, Yu X, et al. Grain yield responses to moisture regimes in rice. Theor Appl Genet. 2007;115:847–55.
  27. 27. Ahmad MS, Wu B, Wang H, Kang D. Field screening of rice germplasm (Oryza sativa L. ssp. japonica) based on days to flowering for drought escape. Plants. 2020;9(5):609. https://doi.org/10.3390/plants9050609
  28. 28. Bhattarai U, Subudhi PK. Genetic diversity, population structure and marker-trait association for drought tolerance in US rice germplasm. Plants. 2019;8(12):530. https://doi.org/10.3390/plants8120530
  29. 29. Bhandari A, Sandhu N, Bartholome J, Hamadoun T, Vi C, Ahmadi N, et al. Genome-wide association study for yield and yield-related traits under reproductive stage drought in a diverse indica-aus rice panel. Rice. 2020;13:53. https://doi.org/10.1186/s12284-020-00411-2
  30. 30. Lamichhane N, Dhami U, Bhandari S, et al. Screening of drought-tolerant rice landraces using various drought indices in Nepal. Sci Rep. 2025;15:45752. https://doi.org/10.1038/s41598-025-28661-8
  31. 31. Hussain T, Hussain N, Ahmed M, Nualsri C, Duangpan S. Responses of lowland rice genotypes under terminal water stress and identification of drought tolerance to stabilize rice productivity in Southern Thailand. Plants. 2021;10:2565. https://doi.org/10.3390/plants10122565
  32. 32. Adhikari M, Adhikari NR, Sharma S, Gairhe J, Bhandari RR, Paudel S. Evaluation of drought tolerant rice cultivars using drought tolerant indices under water stress and irrigated condition. Am J Clim Change. 2019;8:228–36. https://doi.org/10.4236/ajcc.2019.82013
  33. 33. Mau YS, Ndiwa AS, Oematan SS, Markus JE. Drought tolerance indices for selection of drought tolerant, high yielding upland rice genotypes. Aust J Crop Sci. 2019;13:170–8. https://doi.org/10.21475/ajcs.19.13.01.p1778
  34. 34. Hooshmandi B. Evaluation of tolerance to drought stress in wheat genotypes. Idesia (Arica). 2019;37(2):37–43. https://doi.org/10.4067/S0718-34292019000200037
  35. 35. Shrestha P, Kandel BP, Dhakal M, Karanjit R, Shrestha P, Poudel A. Evaluation of drought tolerance performance of rice landraces in the mid-hills of Nepal using drought tolerant indices. Discov Appl Sci. 2026;8:272. https://doi.org/10.1007/s42452-026-08316-7
  36. 36. Lekshmy VS, Vijayaraghavareddy P, Nagashree AN, Ramu VS, Ramegowda V, Makarla U, et al. Induction of acquired tolerance through gradual progression of drought is the key for maintenance of spikelet fertility and yield in rice under semi-irrigated aerobic conditions. Front Plant Sci. 2021;11:632919. https://doi.org/10.3389/fpls.2020.632919
  37. 37. Eragam A, Mohapatra A, Shukla V, Kadumuri RV, George AP, Putta L, et al. Panicle transcriptome of high-yield mutant indica rice reveals physiological mechanisms and novel candidate regulatory genes for yield under reproductive stage drought stress. BMC Plant Biol. 2023;23:493. https://doi.org/10.1186/s12870-023-04507-1
  38. 38. Dixit S, Singh A, Cruz MTS, Maturan PT, Amante M, Kumar A. Multiple major QTL lead to stable yield performance of rice cultivars across varying drought intensities. BMC Genet. 2014;15:16. https://doi.org/10.1186/1471-2156-15-16
  39. 39. Sandhu N, Dixit S, Swamy BPM, Vikram P, Venkateshwarlu C, Catolos M, et al. Identification and mapping of stable QTL with main and epistasis effect on rice grain yield under drought. Mol Breed. 2014;33:1–15. https://doi.org/10.1186/1471-2156-15-63
  40. 40. Blum A. Plant breeding for water-limited environments. New York: Springer; 2011. https://doi.org/10.1007/978-1-4419-7491-4

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