Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp9 (2026): Recent Advances in Agriculture

Heterosis-driven breeding in chilli (Capsicum annuum L.) across three diverse agro-climatic regions of Himachal Pradesh

DOI
https://doi.org/10.14719/pst.15753
Submitted
29 May 2026
Published
23-10-2026

Abstract

Chilli has immense global importance because of its export value and medicinal properties. The present study focused on the development and evaluation of high-yielding and resilient cultivars of chilli (Capsicum annuum L.) across three distinct locations (E1-University of Horticulture and Forestry (UHF), Nauni; E2-Regional Horticultural Research and Training Station (RHR&TS), DhaulaKuan, Sirmour; E3-RHR&TS, Bajaura, Kullu), each specified by varying altitude and temperature conditions. A total of 21 hybrids and 7 parents were evaluated during 2021–22 and 2022–23 using a randomised complete block design (RCBD) in a half-diallel mating design. In this study, the assessed genotypes showed wide variability and significant variations. Crosses viz., Him Palam Mirch 2 × Punjab Guchhedar and Him Palam Mirch 2 × Him Palam Mirch 1 were found promising based on per se performance. The highest standard heterosis was exhibited by Him Palam Mirch 2 × Punjab Guchhedar and the highest heterobeltiosis was exhibited by Pusa Sadabahar × DKC-8 across all the environments and pooled analysis for fruit yield and other yield-associated characters. For quality characters such as oleoresin (%) and total soluble solids (TSS) (°B), hybrids viz., Pusa Jwala × Pusa Sadabahar and Pusa Jwala × DKC-8 outperformed other hybrids across all the environments. Therefore, these hybrids can be utilised effectively for their hybrid vigour to develop high-yielding chilli hybrids with superior quality traits and multi-location heterosis evaluation permits breeders to identify hybrids with consistent performance.

References

  1. 1. Pickersgill B. Cytogenetics and evolution of Capsicum. In: Gupta PK, Tsuchiya T, editors. Chromosome engineering in plants: genetics, breeding, evolution, part B. Amsterdam: Elsevier; 1991. p. 139–60. https://doi.org/10.1016/B978-0-444-88260-8.50013-6
  2. 2. Bosland PW. Chiles: a diverse crop. HortTechnology. 1992;2(1):6–10. https://doi.org/10.21273/HORTTECH.2.1.6
  3. 3. Food and Agriculture Organisation of the United Nations (FAO). FAOSTAT database. FAO; 2021. http://www.faostat.fao.org/
  4. 4. Paril J, Reif J, Fournier-Level A, Pourkheirandish M. Heterosis in crop improvement. Plant J. 2024;117(1):23–32. https://doi.org/10.1111/tpj.16488
  5. 5. Zhang Q, Huang S, Soladoye OP, Zhang Y, Fu Y. Preparation, pungency and bioactivity of capsaicin: a review. Int J Food Sci Technol. 2024;59(9):6659–87. https://doi.org/10.1111/ijfs.17291
  6. 6. Zewdie Y, Bosland PW, Steiner R. Combining ability and heterosis for capsaicinoids in Capsicum pubescens. HortScience.2001;36(7):1315–7. https://doi.org/10.21273/HORTSCI.36.7.1315
  7. 7. Bosland PW, Votava EJ. Peppers: vegetable and spice Capsicums. Wallingford: CABI Publishing; 1999.
  8. 8. Islam MA, Sharma SS, Sinha P, Negi MS, Neog B, Tripathi SB. Variability in capsaicinoid content in different landraces of Capsicum cultivated in north eastern India. Sci Hortic. 2015;183:66–71. https://doi.org/10.1016/j.scienta.2014.12.011
  9. 9. Dhaliwal MS. Chilli. In: Handbook of vegetable crops. New Delhi: Kalyani Publishers; 2017. p. 64–74.
  10. 10. Ali MM, Khalid NI, Wondi MH, Haris NIN, Ahmad M, Azman PN. Exploring the nutritional values, volatile compounds, health benefits and potential food products of chilli (Capsicum annuum): A comprehensive review. Food Chem. 2025;490:145091. https://doi.org/10.1016/j.foodchem.2025.145091
  11. 11. Fernanda RP, Mariana CF, Bruno NP, do Amaral PJS, Miriam DH. Spice oleoresins as value-added ingredient for food industry: Recent advances and perspectives. Trends Food Sci Technol. 2022;122:123–39. https://doi.org/10.1016/j.tifs.2022.02.010
  12. 12. Li H, Jin X, Suh OS, Choi Y, Jin CF, Kim S, et al. Effect of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk colour of laying hens. Asian-Australas J Anim Sci. 2012;25(11):1605–10. https://doi.org/10.5713/ajas.2012.12235
  13. 13. do Rêgo ER, do Rêgo MM, Finger FL, Cruz CD, Casali VW. A diallel study of yield components and fruit quality in chilli pepper (Capsicum baccatum). Euphytica. 2009;168(2):275–87. https://doi.org/10.1007/s10681-009-9947-y
  14. 14. Hasanuzzaman M, Hakim MA, Fersdous J, Islam MM, Rahman L. Combining ability and heritability analysis for yield and yield contributing characters in chilli (Capsicum annuum) landraces. Plant Omics. 2012;5(4):337–44.
  15. 15. Meena OP, Dhaliwal MS, Jindal SK. Heterosis breeding in chilli pepper by using cytoplasmic male sterile lines for high-yield production with special reference to seed and bioactive compound content under temperature stress regimes. Sci Hortic.2019;256:108536. https://doi.org/10.1016/j.scienta.2019.108536
  16. 16. Rodrigues R, Gonçalves LS, Bento CS, Sudré CP, Robaina RR, Amaral Júnior AT. Combining ability and heterosis for agronomic traits in chilli pepper. Hortic Bras. 2012;30(2):226–33. https://doi.org/10.1590/S0102-05362012000200016
  17. 17. Pessoa AM, Rêgo ER, Silva BR, Rêgo MM. Performance of pepper cultivars, for fresh market, based on fruit traits. Hortic Bras. 2023;41:e2495. https://doi.org/10.1590/s0102-0536-2023-e2495
  18. 18. Shan Y, Zhang L, Liu W, Chen B, Zhao K. Correlation analysis and path analysis of agronomic characters and yield of pepper. Chin Agric Sci Bull. 2025;41(1):48–54. https://doi.org/10.11924/j.issn.1000-6850.casb2024-0127
  19. 19. India Meteorological Department. Annual and seasonal mean temperature of India. New Delhi: Government of India; 2017. https://data.gov.in/catalog/annual-and-seasonal-mean-temperature-india
  20. 20. Harvell KP, Bosland PW. The environment produces a significant effect on pungency of chiles. HortScience. 1997;32(7):1292. https://doi.org/10.21273/HORTSCI.32.7.1292
  21. 21. Mansour-Gueddes SB, Tarchoun N, Teixeira da Silva JA, Saguem S. Agronomic and chemical evaluation of seven hot pepper (Capsicum annuum L.) populations grown in an open field. Fruit Veg Cereal Sci Biotech. 2010;4(1):93–7.
  22. 22. Saha SR, Hossain MM, Rahman MM, Kuo CG, Abdullah S. Effect of high temperature stress on the performance of twelve sweet pepper genotypes. Bangladesh J Agric Res. 2010;35(3):525–34. https://doi.org/10.3329/bjar.v35i3.6459
  23. 23. Gyalai IM, Kovács A, Szabó Z. Challenges and solutions in hand pollination for hybrid pepper (Capsicum annuum L.) seed production: a review. Planta. 2026;263:138. https://doi.org/10.1007/s00425-026-05012-z
  24. 24. Aloni B, Peet M, Pharr M, Karni L. The effect of high temperature and high atmospheric CO2 on carbohydrate change in bell pepper (Capsicum annuum) pollen in relation to its germination. Physiol Plant. 2001;112(4):505–12. https://doi.org/10.1034/j.1399-3054.2001.1120407.x
  25. 25. Erickson AN, Markhart AH. Flower production, fruit set and physiology of bell pepper during elevated temperature and vapor pressure deficit. J Am Soc Hortic Sci. 2001;126(6):697–702. https://doi.org/10.21273/JASHS.126.6.697
  26. 26. Singh P, Cheema DS, Dhaliwal MS, Garg N. Heterosis and combining ability for earliness, plant growth, yield and fruit attributes in hot pepper (Capsicum annuum L.) involving genetic and cytoplasmic-genetic male sterile lines. Sci Hortic. 2014;168:175–88. https://doi.org/10.1016/j.scienta.2014.01.028
  27. 27. Tanksley SD. High rates of cross-pollination in chile pepper. HortScience. 1984;19(4):580–2.
  28. 28. Sreelathakumary I, Rajamony L. Variability, heritability and genetic advance in bird pepper. Capsicum Eggplant Newsl. 2003;22:51–4.
  29. 29. Borgohain R, Devi J, Sarma RN. Intra and interspecific genetic diversity exploration in chilli (Capsicum spp.) using morphological and randomly applied polymorphic DNA markers. Indian J Agric Sci. 2005;75(9):582–5.
  30. 30. Gupta AM, Singh D, Kumar A. Genetic variability, genetic advance and correlation studies in chilli (Capsicum annuum). Indian J Agric Sci. 2009;79(1):59–61.
  31. 31. Hradesh, Kumar S, Sharma V, Singh R, Thakur A. Heterosis studies for yield-related traits in cucumber (Cucumis sativus L.). Plant Sci Today. 2026;13(2):1–7. https://doi.org/10.14719/pst.9524
  32. 32. Patel MP, Patel AR, Patel JB, Patel JA. Heterosis for green fruit yield and its components in chilli (Capsicum annuum var. longicum) over environments. Electron J Plant Breed. 2010;1(6):1443–53.
  33. 33. Patil BT, Bhalekar MN, Shinde KG. Heterosis studies in chilli (Capsicum annuum L.) for earliness, growth and green fruit yield. Veg Sci. 2012;39(1):73–5.
  34. 34. Sharma VK, Punetha S, Sharma BB. Heterosis studies for earliness, fruit yield and yield attributing traits in bell pepper. Afr J Agric Res. 2013;8(29):4088–98. https://doi.org/10.5897/AJAR2013.6870
  35. 35. Khalil MR, Hatem MK. Study on combining ability and heterosis of yield and its components in pepper (Capsicum annum L.). Alex J Agric Res. 2014;59(1):61–71.
  36. 36. Directorate of Extension Education. Package of practices for vegetable crops. Solan: Dr YS Parmar University of Horticulture and Forestry; 2014. p. 26–30.
  37. 37. Hayes HK, Immer FR, Smith DC. Methods of plant breeding. 2nd ed. New York: McGraw-Hill; 1955. p. 52–65.
  38. 38. Fonseca S, Patterson FL. Hybrid vigour in a seven parent diallel cross in common winter wheat (Triticum aestivum L.). Crop Sci. 1968;8(1):85–8. https://doi.org/10.2135/cropsci1968.0011183X000800010025x
  39. 39. Berhate SG, Musmade AM, Bhalekar MN, Patil BT. Heterosis for green fruit yield and its contributing characters in chilli (Capsicum annuum L.). Bioinfolet. 2013;10(4):1506–15.
  40. 40. Choi S, Lee J, Kim H, Park M. Development of a speed breeding protocol with flowering gene investigation in pepper (Capsicum annuum). Front Plant Sci. 2023;14:1151765. https://doi.org/10.3389/fpls.2023.1151765
  41. 41. Sahmat SS, Rafii MY, Oladosu Y, Jusoh M, Hakiman M, Mohidin H. Unravelling the dynamics of genotype and environment interactions on chilli (Capsicum annuum L.) yield-related attributes in soilless planting systems. Sci Rep. 2024;14:1698. https://doi.org/10.1038/s41598-023-50381-0
  42. 42. Lata H, Sharma A, Thakur H, Thakur A, Rana RS, Kaur M. Heterosis and combining ability vis-à-vis association for green fruit yield and component traits involving male sterile lines in chilli (Capsicum annuum) under wet temperate zone of North Western Himalayas. Plant Breed. 2023;142(4):547–62. https://doi.org/10.1111/pbr.13114
  43. 43. Arti D, Mehta DK, Vikram A, Kumar R, Aralikatti O. Combining ability and heterosis in bell pepper grown in the north-western hills of India. Indian J Hortic. 2023;80(2):129–35. https://doi.org/10.58993/ijh/2023.80.2.1
  44. 44. Prajapati DB, Agalodia AV. Heterosis and inbreeding depression in chilli (Capsicum annuum L.). J Spices Aromat Crops. 2011;20(2):72–6.
  45. 45. Naves ER, Scossa F, Araujo WL, Nunes-Nesi AR, Fernie A, Zsófi A, et al. Heterosis and reciprocal effects for agronomic and fruit traits in Capsicum pepper hybrids. Sci Hortic. 2022;295:110821. https://doi.org/10.1016/j.scienta.2021.110821
  46. 46. Reddy CN, Reddy KM, Sridevi O, Salimath PM. Chilli (Capsicum annuum L.) breeding in India: An overview. SABRAO J Breed Genet. 2014;46(2):160–73.
  47. 47. Aishwarya KS, Sood M, Hanchinamani CN, Dhananjaya BN, Sangeetha CG. Heterosis and combining ability studies for yield and quality traits in pepper (Capsicum annuum L.). Int J Res Agron. 2026;9(3):490–8. https://doi.org/10.33545/2618060X.2026.v9.i3g.5302
  48. 48. Wang S, Liu Y, Zhang X, Zhao J, Li Y, Wang C, et al. Identification of major loci governing 13 agronomic traits and the fine-mapping of CaSUN29 regulating fruit length in pepper. Mol Hortic. 2025;5:64. https://doi.org/10.1186/s43897-025-00179-7
  49. 49. Medeiros AM, Rodrigues R, Gonçalves LS, Sudré CP, Santos HO, Silva MS. Gene effect and heterosis in Capsicum baccatum var. pendulum. Cienc Rural. 2014;44(6):1031–6. https://doi.org/10.1590/0103-8478cr20130816
  50. 50. Ro N, Oh H, Ko HC, Yi J, Na YW, Haile M. Exploring genomic regions associated with fruit traits in pepper: insights from multiple GWAS models. Int J Mol Sci. 2024;25(21):11836. https://doi.org/10.3390/ijms252111836
  51. 51. Fernie AR, Bachem CW, Helariutta Y, Neuhaus HE, Prat S, Ruan YL, et al. Synchronisation of developmental, molecular and metabolic aspects of source–sink interactions. Nat Plants. 2020;6(2):55–66. https://doi.org/10.1038/s41477-020-0590-x
  52. 52. Chen ZJ. Genomic and epigenetic insights into the molecular bases of heterosis. Nat Rev Genet. 2013;14(7):471–82. https://doi.org/10.1038/nrg3503
  53. 53. Prasath D, Ponnuswami V. Heterosis and combining ability for morphological, yield and quality characters in paprika type chilli hybrids. Indian J Hortic. 2008;65(4):441–5.
  54. 54. Chaudhary A, Kumar R, Solankey SS. Estimation of heterosis for yield and quality components in chilli (Capsicum annuum L.). Afr J Biotechnol. 2013;12(47):6605–10. https://doi.org/10.5897/AJB2013.12108
  55. 55. Ali M, Khan A, Shah A, Hussain I, Jan SU, Ahmed N, et al. Heterosis and combining ability analysis of Phytophthora capsici induced root rot resistance and horticultural traits in Capsicum annuum. Sci Rep. 2025;15(1):37590. https://doi.org/10.1038/s41598-025-17939-6
  56. 56. Kumar RL, Sridevi O, Kage U, Salimath PM, Madalageri D, Natikar P. Heterosis studies in chilli (Capsicum annuum L.). Int J Hortic. 2014;4:40–3. https://doi.org/10.5376/ijh.2014.04.0007
  57. 57. Bala S, Karak C, Mandal AK, Chattopadhyay A. Breeding chili pepper for simultaneous improvement in leaf curl virus and anthracnose disease tolerance and commercially important traits. Int J Veg Sci. 2024;30(1):91–109. https://doi.org/10.1080/19315260.2023.2301348
  58. 58. Ghosh A, Pugalendhi L. Studies on the improvement of chilli (Capsicum annuum L.) cv. Co-4 for quality characters through hybridisation. Electron J Plant Breed. 2012;3(2):811–7.
  59. 59. Verma V. Genetic analysis of yield and contributing traits in chilli (Capsicum annuum L.). Solan: Dr Yashwant Singh Parmar University of Horticulture and Forestry; 2022.
  60. 60. do Rêgo ER, do Rêgo MM, Cruz CD, Finger FL, Casali VW. Phenotypic diversity, correlation and importance of variables for fruit quality and yield traits in Brazilian peppers (Capsicum baccatum). Genet Resour Crop Evol. 2011;58(6):909–18. https://doi.org/10.1007/s10722-010-9628-7
  61. 61. Guijarro-Real C, Adalid-Martínez AM, Pires CK, Ribes-Moya AM, Fita A, Rodríguez-Burruezo A. The effect of the varietal type, ripening stage and growing conditions on the content and profile of sugars and capsaicinoids in Capsicum peppers. Plants.2023;12(2):231. https://doi.org/10.3390/plants12020231
  62. 62. Rohini N, Lakshmanan V. Evaluation studies of hot pepper hybrids (Capsicum annuum L.) for yield and quality characters. Electron J Plant Breed. 2017;8(2):643–51. https://doi.org/10.5958/0975-928X.2017.00098.9
  63. 63. Lata H, Sharma A. Exploitation of heterosis in chilli using genetic male sterile lines for red fruit yield with special reference to high rainfall conditions. Sci Rep. 2024;14:23061. https://doi.org/10.1038/s41598-024-73728-7

Downloads

Download data is not yet available.