Two species of Strobilanthes Blume (Acanthaceae), S. anisophylla (Wall. ex Hook.) T. Anderson and S. sabiniana (Wall. ex Lindl.) Nees are added as a new distribution records for Assam from Manas Biosphere Reserve. Detailed description, taxonomic notes, geographical distribution map and habitat are provided, accompanied by photographic images to facilitate easy identification of the species.