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Plant Science Today (PST)

Research Articles

Vol. 9 No. sp2 (2022)

In vitro studies on quality assessment and ethnobotany of Acampe rigida (Buch.-Ham. ex Sm.) P.F.Hunt encountered in Ultapani Forest Range, Assam

DOI
https://doi.org/10.14719/pst.1727
Submitted
12 February 2022
Published
06-07-2022

Abstract

Acampe rigida (Buch.-Ham.ex Sm.) P.F.Hunt (Family: Orchidaceae), locally known as ‘Miral Baha’ among the Santhal community of Ultapani Forest Range, BTR, Assam, blooms in the month of July to November. The present study was aimed to carry out the ethnobotanical studies, macroscopic, pharmacognostic, preliminary phytochemical and heavy metal analysis of A. rigida. Apart from being an eye-catching ornamental plant, it has a number of medicinal potentialities as folk medicine among the Santhal community where the leaves are used for recovering throat cancer and epilepsy and the paste helps in relieving rheumatism, healing wound as tonic to strengthen the body and increase blood in the body. Parenchyma cells, stomata, fibre were observed in the microscopic examination of powdered plant part. The pulverised leaves colour, odour, flavor/taste and texture were all satisfactory. Preliminary phytochemical screening of A. rigida leaves aqueous extract confirmed the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids, saponins, tannins, terpenoids, whereas phlobatannins was absent. Heavy metal tests revealed that the samples were free from bismuth, cadmium and lead. The present studies might be significant to establish the pharmacognostic and phytochemical standards of this orchid species and may help in the identification, to ensure quality and purity and standardization of the herbal drug.

References

  1. Basumatary N. Collection identification and in vitro propagation of orchid species of chirang reserve forest under haltugaon forest division of Kokrajhar district (Thesis). Botany: Gauhati University; 2004.
  2. Pant B. Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. Afr J Plant Sci. 2013;7(10):448-67. https://doi.org/10.5897/AJPS2013.1031
  3. Sohag SI, Hoque MM, Huda MK. Phytochemical screening and antioxidant activity of rare medicinal orchid Luisia zeylanica Lindl. J Pharmacogn Phytochem. 2017;6(4):688-92.
  4. Hinsley A, De Boer HJ, Fay MF, Gale SW, Gardiner LM, Gunasekara RS et al. A review of the trade in orchids and its implications for conservation. Bot J Linn Soc. 2018;186(4):435-55. https://doi.org/10.1093/botlinnean/box083
  5. Singh SK, Agarwala DK, Jalal JS, Dash SS, Mao AA, Singh P, editors. Orchids of India: A Pictorial Guide. BSI, Kolkata, India; 2019.
  6. Sharma A, Pathak P. The budding potential of orchids in the cosmeceutical sector: role of orchids in skincare and health. J Orchid Soc India. 2020;34:79-85.
  7. Gogoi K, Das R, Yonzone R. Orchids of Assam, North East India-An annotated checklist. Int J of Pharm Life Sci. 2015;6(1):4123-56.
  8. Das S, Baruah S, Goyal AK. Reduction In Orchid diversity In Ultapani Forest Range of Kokrajhar District of Assam. J Emerg Technol Innov Res. 2021;8(5):g677-84.
  9. Aswandi A, Kholibrina CR. Ethnomedicinal properties of orchidaceae by local communities in Lake Toba region, North Sumatra, Indonesia. IOP Conf Ser: Earth Environ Sci. 2021;914(1):012056. https://doi.org/10.1088/1755-1315/914/1/012056
  10. Tsering J, Tam N, Tag H, Gogoi BJ, Apang O. Medicinal orchids of Arunachal Pradesh: a review. Bull Arunachal Forest Res. 2017;32(1&2):1-16.
  11. Jain SK, Rao RR, editors. Field and Herbarium Methods. Today and Tomorrow Publishers. New Delhi;1977.
  12. Aslam I, Iqbal J, Peerzada S, Afridi MS, Ishtiaq S. Microscopic investigations and pharmacognostic techniques for the standardization of Caralluma edulis (Edgew.) Benth. ex Hook. f. Microsc Res Tech. 2019;82(11):1891-1902. https://doi.org/10.1002/jemt.23357
  13. Brahma S, Mochahary B, Kalita M, Goyal AK. Pharmacognostic and physicochemical characterisation of potential plants for anti-diabetic herbal formulations. Plant Sci Today. 2022. https://doi.org/10.14719/ pst.1704
  14. Mochahary B, Brahma S, Kalita M, Goyal AK. Characterisation of indigenous plants for herbal formulations preparation based on pharmacognostic and physiochemical data. Plant Sci Today. 2022. https://doi.org/10.14719/pst.1709
  15. Trease GE, Evans WC, editors. Pharmacognosy. 11th ed. Brailliar Tiridel Can. Macmillian Publishers. 1989; pp.10-15.
  16. Harbrone JB, editor. Phytochemical Methods. Chapman and Hall Ltd. London; 1973; pp.49-188.
  17. Kolawole OM. Studies on the efficacy of Bridelia ferruginea Benth. bark extract in reducing the coliform load and BOD of domestic wastewater. Ethnobot leafl. 2006;10:228-38.
  18. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685-88. https://doi.org/10.5897/AJB2005.000-3127
  19. Goyal AK, Middha SK, Sen A. Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris" Vittata" methanolic leaf extract. J Nat Pharm. 2010;1(1):40-45. https://doi.org/10.4103/2229-5119.73586
  20. Ranjith D. Fluorescence analysis and extractive values of herbal formulations used for wound healing activity in animals. J Med Plants Stud. 2018;6(2):189-92.
  21. Chuakul W. Ethnomedical uses of Thai orchidaceous plants. Mohidol Univ J Pharm Sci. 2002;29(3-4):41-45.
  22. Teoh ES. Secondary metabolites of plants. In:Medicinal orchids of Asia. Springer, Cham. 2016; pp. 59-73. https://doi.org/10.1007/978-3-319-24274-3
  23. Pruyn ML. Parenchyma. eLS. 2001;1-8. https://doi.org/10.1002/9780470015902.a0002083.pub2
  24. Mukhi S, Bose A, Panda P, Rao MM. Pharmacognostic, physicochemical and chromatographic characterization of Samasharkara Churna. J Ayurveda Integr Med. 2016;7(2):88-99. https://doi.org/10.1016/j.jaim.2015.11.004
  25. Hussain G, Rasul A, Anwar H, Aziz N, Razzaq A, Wei W, Ali M, Li J, Li X. Role of plant derived alkaloids and their mechanism in neurodegenerative disorders. Int J Biol Sci. 2018;14(3):341-57. https://doi.org/10.7150/ijbs.23247
  26. Lee S, Kim DC, Baek HY, Lee KD, Kim YC, Oh H. Anti-neuroinflammatory effects of tryptanthrin from Polygonum tinctorium Lour. in lipopolysaccharide-stimulated BV2 microglial cells. Arch Pharm Res. 2018;41(4):419-30. https://doi.org/10.1007/s12272-018-1020-8
  27. Kurek J, editor. Introductory chapter: alkaloids-their importance in nature and for human life. In Alkaloids-Their Importance in Nature and Human Life. London, UK: IntechOpen; 2019. https://doi.org/10.5772/intechopen.85400
  28. Adamski Z, Blythe LL, Milella L, Bufo SA.. Biological activities of alkaloids: from toxicology to pharmacology. Toxins. 2020;12(4):210. https://doi.org/10.3390/toxins12040210
  29. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Sci World J. 2013;162750 . https://doi.org/10.1155/2013/162750
  30. Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016;5. https://doi.org/10.1017/jns.2016.41
  31. Ruiz-Cruz S, Chaparro-Hernández S, Hernández-Ruiz KL, Cira-Chávez LA, Estrada-Alvarado MI, Gassos Ortega LE et al. Flavonoids: Important biocompounds in food. Flavonoids-from biosynthesis to human health. InTech. 2017;353-69. https://doi.org/10.5772/67864
  32. Mann J. Dietary carbohydrate: relationship to cardiovascular disease and disorders of carbohydrate metabolism. Eur J Clin Nutr. 2007;61(1):S100-S111. https://doi.org/10.1038/sj.ejcn.1602940
  33. Maureen Z, Beth S, editors. The Functions of Carbohydrates in the Body. An Introduction to Nutrition; 2012; pp.165-232.
  34. Kanter M. High-quality carbohydrates and physical performance: Expert panel report. Nutr Today. 2018;53(1):35-39. https://doi.org/10.1097/NT.0000000000000238
  35. Maridass M, Hussain MI, Raju G. Phytochemical survey of orchids in the Tirunelveli Hills of South India. Ethnobot leafl. 2008;12:705-12.

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