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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp9 (2026): Recent Advances in Agriculture

Optimising foliar nano calcium oxide application for improved growth, yield and post-harvest storability of tomato (Solanum lycopersicum L.)

DOI
https://doi.org/10.14719/pst.17035
Submitted
26 July 2026
Published
29-10-2026

Abstract

A pot experiment was conducted to investigate the dose-dependent response of tomato (Solanum lycopersicum L.) to foliar application of nano calcium oxide (nano CaO). The study included 13 treatments comprising 11 nano CaO concentrations (100–1500 ppm), a calcium nitrate (CaNO3) treatment and an untreated control, laid out in a completely randomised design (CRD) with 3 replications. Foliar sprays were applied at 30 and 45 DAT. Most traits differed significantly (p < 0.001), showing a hormesis-type response with optimum performance at 600 ppm nano CaO. The 600 ppm treatment consistently produced the tallest plants, highest dry weight, soil plant analysis development (SPAD) values, fruit size, yield (2.23 kg plant-1), shelf life (14.5 days) and fruit firmness (3.21 kg cm-2), while recording the lowest percent of physiological loss in weight (10.1 after 12 days). Compared with calcium nitrate, nano CaO at 600 ppm increased yield by 18.62 % using less than one-third of the calcium concentration. Quadratic regression predicted an optimum nano CaO concentration of 550–635 ppm, corroborating the observed optimum, while correlation analysis identified fruit volume and plant dry weight as the strongest predictors of yield and post-harvest storability. These findings indicate that foliar application of nano CaO at approximately 600 ppm can improve tomato growth, yield and postharvest storability at a substantially lower calcium concentration than calcium nitrate, while the decline in performance at higher concentrations emphasises the need for dose optimisation.

References

  1. 1. Duro JA, Lauk C, Kastner T, Erb KH, Haberl H. Global inequalities in food consumption, cropland demand and land-use efficiency: A decomposition analysis. Glob Environ Change. 2020;64:102124. https://doi.org/10.1016/j.gloenvcha.2020.102124
  2. 2. Tian X, Engel BA, Qian H, Hua E, Sun S, Wang Y. Will reaching the maximum achievable yield potential meet future global food demand? J Clean Prod. 2021;294:126285. https://doi.org/10.1016/j.jclepro.2021.126285
  3. 3. Ashraf SA, Siddiqui AJ, Abd Elmoneim OE, Khan MI, Patel M, Alreshidi M, et al. Innovations in nanoscience for the sustainable development of food and agriculture with implications on health and environment. Sci Total Environ. 2021;768:144990. https://doi.org/10.1016/j.scitotenv.2021.144990
  4. 4. Neme K, Nafady A, Uddin S, Tola YB. Application of nanotechnology in agriculture, postharvest loss reduction and food processing: Food security implication and challenges. Heliyon. 2021;7:e08539. https://doi.org/10.1016/j.heliyon.2021.e08539
  5. 5. Prasad R, Bhattacharyya A, Nguyen QD. Nanotechnology in sustainable agriculture: Recent developments, challenges and perspectives. Front Microbiol. 2017;8:1014. https://doi.org/10.3389/fmicb.2017.01014
  6. 6. Nikolaou G, Neocleous D, Christou A, Polycarpou P, Kitta E, Katsoulas N. Energy and water related parameters in tomato and cucumber greenhouse crops in semiarid Mediterranean regions. A review, part II: Irrigation and fertigation. Horticulturae. 2021;7:548. https://doi.org/10.3390/horticulturae7120548
  7. 7. Wang D, Wang Y, Lv Z, Pan Z, Wei Y, Shu C, et al. Analysis of nutrients and volatile compounds in cherry tomatoes stored at different temperatures. Foods. 2023;12(1):6. https://doi.org/10.3390/foods12010006
  8. 8. Collins EJ, Bowyer C, Tsouza A, Chopra M. Tomatoes: An extensive review of the associated health impacts of tomatoes and factors that can affect their cultivation. Biology. 2022;11(2):239. https://doi.org/10.3390/biology11020239
  9. 9. Camara M, Fernandez-Ruiz V, Sanchez-Mata MC, Camara RM, Dominguez L, Sesso HD. Scientific evidence of the beneficial effects of tomato products on cardiovascular disease and platelet aggregation. Front Nutr. 2022;9:849841. https://doi.org/10.3389/fnut.2022.849841
  10. 10. Topcu Y, Nambeesan SU, van der Knaap E. Blossom-end rot: A century-old problem in tomato (Solanum lycopersicum L.) and other vegetables. Mol Hortic. 2022;2:1. https://doi.org/10.1186/s43897-021-00022-9
  11. 11. Watanabe T, Tomizaki R, Watanabe R, Maruyama H, Shinano T, Urayama M, et al. Ionomic differences between tomato introgression line IL8-3 and its parent cultivar M82 with different trends to the incidence of blossom-end rot. Sci Hortic. 2021;287:110266. https://doi.org/10.1016/j.scienta.2021.110266
  12. 12. Tanveer K, Gilani S, Hussain Z, Ishaq R, Adeel M, Ilyas N. Effect of salt stress on tomato plant and the role of calcium. J Plant Nutr. 2019;43:28–35. https://doi.org/10.1080/01904167.2019.1659324
  13. 13. Islam MZ, Mele MA, Baek JP, Kang HM. Cherry tomato qualities affected by foliar spraying with boron and calcium. Hortic Environ Biotechnol. 2016;57:46–52. https://doi.org/10.1007/s13580-016-0097-6
  14. 14. Gupta S, Kaur N, Kant K, Jindal P, Ali A, Naeem M. Calcium: A master regulator of stress tolerance in plants. S Afr J Bot. 2023;163:580–94. https://doi.org/10.1016/j.sajb.2023.10.047
  15. 15. Hamza M, Abbas M, Abd Elrahman A, Helal M, Shahba M. Conventional versus nano calcium forms on peanut production under sandy soil conditions. Agriculture. 2021;11:767. https://doi.org/10.3390/agriculture11080767
  16. 16. Mogazy AM, Mohamed HI, El-Mahdy OM. Calcium and iron nanoparticles: A positive modulator of innate immune responses in strawberry against Botrytis cinerea. Process Biochem. 2022;115:128–45. https://doi.org/10.1016/j.procbio.2022.02.014
  17. 17. Sahin O, Yagcioglu KD, Kadioglu YK, Gunes A. Evaluating ecological nano-calcium from eggshells: Effects on calcium nutrition and oxidative stress in lettuce under saline and boron toxicity. J Plant Growth Regul. 2024;43(12):4416–25. https://doi.org/10.1007/s00344-024-11407-7
  18. 18. Alnaass NS, Agil HK, Ibrahim HK. Use of fertilizers or importance of fertilizers in agriculture. Int J Adv Acad Stud. 2021;3:52–7. https://doi.org/10.33545/27068919.2021.v3.i2a.770
  19. 19. Hou J, Riaz M, Yan L, Lu K, Jiang C. Effect of exogenous L-aspartate nano calcium on root growth, calcium forms and cell wall metabolism of Brassica napus L. NanoImpact. 2022;27:100415. https://doi.org/10.1016/j.impact.2022.100415
  20. 20. Jing T, Li J, He Y, Shankar A, Saxena A, Tiwari A, et al. Role of calcium nutrition in plant physiology: Advances in research and insights into acidic soil conditions-a comprehensive review. Plant Physiol Biochem. 2024;210:108602. https://doi.org/10.1016/j.plaphy.2024.108602
  21. 21. Silvestre-Castaneda L, González-García Y, Pérez-Alvarez M, Cadenas-Pliego G, Juárez-Maldonado A. Calcium nanoparticles plus amino acids improve the antioxidant content and fruit quality of tomato. Scientifica. 2025;2025:8695613. https://doi.org/10.1155/sci5/8695613
  22. 22. Mavuduru Deena Damodhar, Byanna CN, Prasad TNVK, Siva Prasad M, Mukunda Lakshmi L. Nano calcium preharvest spray efficacy in improving tomato (Solanum lycopersicum L.) yield, physicochemical qualities and shelf-life. Int J Adv Biochem Res. 2025;9(9S):1521–4. https://doi.org/10.33545/26174693.2025.v9.i9Ss.5719
  23. 23. Padmaja N, John DS. Preservation of sapota (Manilkara zapota) by edible aloe vera gel coating to maintain its quality. Int J Sci Res. 2014;3:177–9. https://doi.org/10.15373/22778179/August2014/51
  24. 24. Farooq A, Javad S, Jabeen K, Shah AA, Ahmad A, Shah AN, et al. Effect of calcium oxide, zinc oxide nanoparticles and their combined treatments on growth and yield attributes of Solanum lycopersicum L. J King Saud Univ Sci. 2023;35:102647. https://doi.org/10.1016/j.jksus.2023.102647
  25. 25. Menezes de LB, Druzian DM, Oviedo LR, Bonazza GKC, Machado AK, Da Silva WL. In vitro safety profile and phyto-ecotoxicity assessment of the eco-friendly calcium oxide nanoparticles. Chemosphere. 2024;365:143407. https://doi.org/10.1016/j.chemosphere.2024.143407
  26. 26. Weng X, Li H, Ren C, Zhou Y, Zhu W, Zhang S, et al. Calcium regulates growth and nutrient absorption in poplar seedlings. Front Plant Sci. 2022;13:887098. https://doi.org/10.3389/fpls.2022.887098
  27. 27. Kou L, Yang T, Luo Y, Liu X, Huang L, Codling E. Pre-harvest calcium application increases biomass and delays senescence of broccoli microgreens. Postharvest Biol Technol. 2014;87:70–8. https://doi.org/10.1016/j.postharvbio.2013.08.004
  28. 28. Thor K. Calcium-nutrient and messenger. Front Plant Sci. 2019;10:440. https://doi.org/10.3389/fpls.2019.00440
  29. 29. Ali I, Abbasi NA, Hafiz I. Application of calcium chloride at different phenological stages alleviates chilling injury and delays climacteric ripening in peach fruit during low-temperature storage. Int J Fruit Sci. 2021;21:1040–58. https://doi.org/10.1080/15538362.2021.1975607
  30. 30. Dorostkar M, Moradinezhad F, Ansarifar E. Effectiveness of postharvest calcium salts applications to improve shelf life and maintain apricot fruit quality during storage. Rev Fac Nac Agron Medellin. 2022;75:9983–8. https://doi.org/10.15446/rfnam.v75n2.98060
  31. 31. Moradinezhad F, Dorostkar M. Pre-harvest foliar application of calcium chloride and potassium nitrate influences growth and quality of apricot (Prunus armeniaca L.) fruit cv. Shahroudi. J Soil Sci Plant Nutr. 2021;21:1642–52. https://doi.org/10.1007/s42729-021-00468-2
  32. 32. Hussein SA, Al-Doori MF. The effect of spraying with calcium, boron and benzyl adenine on the quantity and quality of yield for strawberry plants (Fragaria × ananassa Duch.) cv. Rubygem. IOP Conf Ser Earth Environ Sci. 2021;910:012066. https://doi.org/10.1088/1755-1315/910/1/012066
  33. 33. Ranjbar S, Ramezanian A, Rahemi M. Nano-calcium and its potential to improve Red Delicious apple fruit characteristics. Hortic Environ Biotechnol. 2020;61:23–30. https://doi.org/10.1007/s13580-019-00168-y
  34. 34. Shi H, Zhou W, Xu Y, He X, He F, Wang Y. Effect of calcium spray at flowering combined with postharvest 1-MCP treatment on the preservation of grapes. Heliyon. 2023;9:e19918. https://doi.org/10.1016/j.heliyon.2023.e19918
  35. 35. Sotiropoulos T, Voulgarakis A, Karaiskos D, Chatzistathis T, Manthos I, Dichala O, et al. Foliar calcium fertilizers impact on several fruit quality characteristics and leaf and fruit nutritional status of the Hayward kiwifruit cultivar. Agronomy. 2021;11:235. https://doi.org/10.3390/agronomy11020235
  36. 36. Rajani D, Padma M, Akhil K, Raj KM. Effect of foliar application of nano CaO, ZnO and boron on fruit quality and nutrient uptake in tomato (Solanum lycopersicum L.). Plant Sci Today. 2026;13(sp5):1–13. https://doi.org/10.14719/pst.15793
  37. 37. Melo RO, Prieto Martinez HE, Rocha BCP, Garcia Junior E. Production and quality of Sweet Grape tomato in response to foliar calcium fertilization. Rev Ceres. 2022;69:48–54. https://doi.org/10.1590/0034-737x202269010007
  38. 38. Huang W, Shi Y, Yan H, Wang H, Wu D, Grierson D, et al. The calcium-mediated homogalacturonan pectin complexation in cell walls contributes to firmness increase in loquat fruit during postharvest storage. J Adv Res. 2023;49:47–62. https://doi.org/10.1016/j.jare.2022.09.009
  39. 39. Zhang L, Wang P, Chen F, Lai S, Yu H, Yang H. Effects of calcium and pectin methylesterase on quality attributes and pectin morphology of jujube fruit under vacuum impregnation during storage. Food Chem. 2019;289:40–8. https://doi.org/10.1016/j.foodchem.2019.03.008
  40. 40. Gao Q, Xiong T, Li X, Chen W, Zhu X. Calcium and calcium sensors in fruit development and ripening. Sci Hortic. 2019;253:412–21. https://doi.org/10.1016/j.scienta.2019.04.069
  41. 41. Yu J, Zhang Y, Liu J, Wang L, Liu P, Yin Z, et al. Proteomic discovery of H₂O₂ response in roots and functional characterization of PutGLP gene from alkaligrass. Planta. 2018;248:1079–99. https://doi.org/10.1007/s00425-018-2940-8
  42. 42. Frempong KEB, Chen Y, Liang L, Lin X. Effect of calcium chloride and 1-methylcyclopropene combined treatment on pectin degradation and textural changes of Eureka lemon during postharvest storage. Curr Res Food Sci. 2022;5:1412–21. https://doi.org/10.1016/j.crfs.2022.08.023
  43. 43. Xu Y, Liu J, Zang N, Yin Z, Wang A. Effects of calcium application on apple fruit softening during storage revealed by proteomics and phosphoproteomics. Hortic Plant J. 2022;8:408–22. https://doi.org/10.1016/j.hpj.2022.03.002
  44. 44. Wu C, Xia N, Wang W. Exogenous calcium on calcium accumulation, uptake and utilization in tomato. Horticulturae. 2025;11:986. https://doi.org/10.3390/horticulturae11080986
  45. 45. Iavicoli I, Leso V, Fontana L, Calabrese EJ. Nanoparticle exposure and hormetic dose-responses: An update. Int J Mol Sci. 2018;19(3):805. https://doi.org/10.2203/dose-response.10-016.Iavicoli
  46. 46. Nascarella MA, Calabrese EJ. A method to evaluate hormesis in nanoparticle dose–responses. Dose Response. 2012;10(3). https://doi.org/10.2203/dose-response.10-025.Nascarella

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