Characterisation of indigenous plants for herbal formulations preparation based on pharmacognostic and physiochemical data

Authors

DOI:

https://doi.org/10.14719/pst.1709

Keywords:

Medicinal plants, Organoleptic, Heavy metal, Alstonia, Centella, Drymaria, Hydrocotyle, Oroxylum, Senna, Solanum, Stephania

Abstract

The use of plant-based drugs has increased considerably in the modern world for their efficiency in managing diseases with lesser side effects than synthetic drugs. The current study was aimed to confirm the identity, quality and purity of some locally available potential medicinal plants such as Alstonia scholaris (bark), Centella asiatica (whole plant), Drymaria cordata (whole plant), Hydrocotyle sibthorpioides (whole plant), Oroxylum indicum (bark), Senna hirsuta (leaf), Senna occidentalis (leaf), Solanum indicum (root), Stephania japonica (tuber) in powdered form. The powdered plant parts were evaluated for preliminary phytochemicals, pharmacognostical studies, physical characteristics and heavy metals. Preliminary phytochemical screening of the different extracts inferred the existence of carbohydrates, phenolics, alkaloids and amino acids while triterpenoids were absent. Microscopical study of the powder revealed the diagnostic qualities such as stone cells, trichomes, stomata, calcium oxalate crystal, fibres, xylem vessel, pitted spiral vessels, etc. The colour, odour, flavour/taste and texture of the pulverized plant were overall acceptable. The physical characteristics which determine the flow rate of the powder with respect to Carr’s index and Hausner’s ratio were found to be good to passable except for Hydrocotyle sibthorpioides (whole plant), Oroxylum indicum (bark), which were not easily passable. The heavy metal test showed the absence of bismuth, cadmium and lead. Thus the present study may serve as a standard reference for the quality control analysis of the herbal drug either singly or in synergy.

Downloads

Download data is not yet available.

References

Xiong F, Guan YS. Cautiously using natural medicine to treat liver problems. World J Gastroenterol. 2017;23(19):3388-95. https://doi.org/10.3748/wjg.v23.i19.3388

Goyal AK, Middha SK, Usha T, Sen A. Analysis of toxic, anti-diabetic and antioxidant potential of Bambusa balcooa Roxb. leaf extracts in alloxan-induced diabetic rats. 3 Biotech. 2017;7:120. https://doi.org/10.1007/s13205-017-0776-8

World Health Organization. WHO global report on traditional and complementary medicine. World Health Organization; 2019.p. 44-45.

Usha T, Pradhan S, Goyal AK, Dhivya S, Kumar HP, Singh MK et al. Molecular simulation-based combinatorial modeling and antioxidant activities of Zingiberaceae family rhizomes. Pharmacogn Mag. 2017;13(3):S715. https://doi.org/10.4103/pm.pm_82_17

Mukhi S, Bose A, Panda P, Rao MM. Pharmacognostic, physicochemical and chromatographic characterization of Samasharkara churna. J Ayurveda Integr Med. 2016;7(2):88-99. https://doi.org/10.1016/j.jaim.2015.11.004

Xie, Z. Practical Traditional Chinese Medicine; Foreign Languages Press: Beijing, China, 2000. p. 260.

Che CT, Wang ZJ, Chow MSS, Lam CWK. Herb-herb combination for therapeutic enhancement and advancement: theory, practice and future perspectives. Molecules 2013;18:5125-41. https://doi.org/10.3390/molecules18055125

Wang S, Hu Y, Tan W, Wu X, Chen R, Cao J, Chen M, Wang Y. Compatibility art of traditional Chinese medicine: from the perspective of herb pairs. J Ethnopharmacol. 2012; 143(2): 412-23. https://doi.org/10.1016/j.jep.2012.07.033

Shi L, Tang X, Dang X, Wang Q, Wang X, He P, Wang Q, Liu L, Liu X, Zhang Y. Investigating herb–herb interactions: The potential attenuated toxicity mechanism of the combined use of Glycyrrhizae radix et rhizoma (Gancao) and Sophorae flavescentis radix (Kushen). J Ethnopharmacol. 2015;165:243-50. https://doi.org/10.1016/j.jep.2015.02.022

Sahoo N, Manchikanti P, Dey S. Herbal drugs: standards and regulation. Fitoterapia. 2010;81(6):462-71. https://doi.org/10.1016/j.fitote.2010.02.001

Pattanayak P, Kumar Hardel D, Mohapatra P. Standardization of Vaisvanara churna: A poly-herbal formulation. Phcog J. 2010; 2:50-59. https://doi.org/10.4103/0974-8490.62957

Kumari R, Dubey GP. Standardization of Ayurvedic Drug-Nyctanthes arbor-tristis, Hippophae salicifolia, Ocimum tenuiflorum and Reinwardtia indica and Combined Herb-Herb. Nepal J Biotechnol. 2020;8(1):36-53. https://doi.org/10.3126/njb.v8i1.30208

Khyade MS, Kasote DM, Vaikos NP. Alstonia scholaris (L.) R. Br. and Alstonia macrophylla Wall. ex G. Don: A comparative review on traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2014;153(1):1-8. https://doi.org/10.1016/j.jep.2014.01.025

Pandey K, Shevkar C, Bairwa K, Kate AS. Pharmaceutical perspective on bioactives from Alstonia scholaris: ethnomedicinal knowledge, phytochemistry, clinical status, patent space and future directions. Phytochem Rev. 2020;19(1):191-33. https://doi.org/10.1007/s11101-020-09662-z

Bello I, Bakkouri AS, Tabana YM, Al-Hindi B, Al-Mansoub MA, Mahmud R, Asmawi M. Acute and sub-acute toxicity evaluation of the methanolic extract of Alstonia scholaris stem bark. Med Sci (Basel). 2016;4(1):4. https://doi.org/10.3390/medsci4010004

Chandra Jagetia G, Shrinath Baliga M. Modulation of antineoplastic activity of cyclophosphamide by Alstonia scholaris in the Ehrlich ascites carcinoma?bearing mice. Journal of Experimental Therapeutics and Oncology. 2003;3(5):272-82. https://doi.org/10.1111/j.1533-869X.2003.01098.x

Pandey K, Shevkar C, Bairwa K, Kate AS. Pharmaceutical perspective on bioactives from Alstonia scholaris: ethnomedicinal knowledge, phytochemistry, clinical status, patent space and future directions. Phytochem Rev. 2020;19:191-33. https://doi.org/10.1007/s11101-020-09662-z

Kumar A, Khan MA, Saxena A, Singh RB, Zaman K, Husain A. Hepatoprotective activity of methanolic extract of stem bark of Alstonia Scholaris (l.) R. Br. Am J Pharmtech Res. 2012;2(2):545-55.

Orhan IE. Centella asiatica (L.) Urban: From traditional medicine to modern medicine with neuroprotective potential. Evidence-based complementary and alternative medicine. 2012;2012: 946259. https://doi.org/10.1155/2012/946259

Joshi K, Chaturvedi P. Therapeutic efficiency of Centella asiatica (L.) Urb. An underutilized green leafy vegetable: an overview. International Journal of Pharma and Bio Sciences. 2013;4(1):135-49.

Prakash V, Jaiswal NI, Srivastava MR. A review on medicinal properties of Centella asiatica. Asian J Pharm Clin Res. 2017;10(10):69-74. https://doi.org/10.22159/ajpcr.2017.v10i10.20760

Gray NE, Magana AA, Lak P, Wright KM, Quinn J, Stevens JF et al. Centella asiatica: Phytochemistry and mechanisms of neuroprotection and cognitive enhancement. Phytochem Rev. 2018;17(1):161-94. https://doi.org/10.1007/s11101-017-9528-y

Oyenihi AB, Langa SO, Mukaratirwa S, Masola B. Effects of Centella asiatica on skeletal muscle structure and key enzymes of glucose and glycogen metabolism in type 2 diabetic rats. Biomed Pharmacother. 2019;112:108715. https://doi.org/10.1016/j.biopha.2019.108715

Torbati FA, Ramezani M, Dehghan R, Amiri MS, Moghadam AT, Shakour N et al. Ethnobotany, Phytochemistry and Pharmacological Features of Centella asiatica: A Comprehensive Review. Adv Exp Med Biol. 2021;1308:451-99. https://doi.org/10.1007/978-3-030-64872-5_25

Dequan L, Gilbert MG. Drymaria Willd. ex J. A. Schultes. In: Beijing, China,.Wu Z, Raven PH, editors. Flora of China (Caryophyllaceae through Lardizabalaceae), Science Press; and St Louis, Missouri, USA: Missouri Botanical Garden Press. 2001, p. 6.

Neogi B, Prasad MN, Rao RR. Ethnobotany of some weeds of Khasi and Garo hills, Meghalaya, Northeastern India. Econ Bot. 1989;43(4):471-79. https://doi.org/10.1007/BF02935921

Nono NR, Nzowa KL, Barboni L, Tapondjou AL. Drymaria cordata (Linn.) Willd. (Caryophyllaceae): Ethnobotany, Pharmacology and Phytochemistry. Adv Biol Chem. 2014;4:160-67. https://doi.org/10.4236/abc.2014.42020

Rao RR. Ethnobotany of Meghalaya: medicinal plants used by Khasi and Garo tribes. Econ Bot. 1981;35(1):4-9. https://doi.org/10.1007/BF02859208

Focho DA, Ndam WT, Fonge BA. Medicinal plants of Aguambu-Bamumbu in the Lebialem highlands, southwest province of Cameroon. Afr J Pharmacy Pharmacol. 2009;3(1):1-13.

Mukherjee PK, Bhattacharya S, Saha K, Giri SN, Pal M, Saha BP. Antibacterial evaluation of Centella asiatica ordata Willd. (Fam. Caryophyllaceae) extract. Phyto Res. 1996;11:249-50. https://doi.org/10.1002/(SICI)1099-1573(199705)11:3<249::AID-PTR69>3.0.CO;2-W

Mukherjee PK, Saha K, Bhattacharya S, Giri SN, Pal M, Saha BP. Studies on antitussive activity of Drymaria cordata Willd. (Caryophyllaceae). J Ethnopharmacol. 1997;56(1):77-80. https://doi.org/10.1016/s0378-8741(97)01512-2

Akindele AJ, Ibe IF, Adeyemi OO. Analgesic and antipyretic activities of Drymaria cordata (Linn.) Willd. (Caryophyllaceae) extract. Afr J Tradit Complement Altern Med. 2012;9(1):25-35. https://doi.org/10.4314/ajtcam.v9i1.4

Huang Q, Zhang S, Huang R, Wei L, Chen Y, Lv S et al. Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam. J Ethnopharmacol. 2013;150(2):568-75. https://doi.org/10.1016/j.jep.2013.09.009

Hazarika I, Mukundan GK, Sundari PS, Laloo D. Journey of Hydrocotyle sibthorpioides Lam.: From traditional utilization to modern therapeutics—A review. Phytother Res. 2021;35(4):1847-71. https://doi.org/10.1002/ptr.6924

Nazmi R, Dillasamola D, Badriyya E, Aldi Y. Anti-inflammatory Activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) Plant Extract on Topical Application. Pharmacogn J. 2021; 13(2):411-17. https://doi.org/10.5530/pj.2021.13.52

Chan SM, Khoo KS, Sekaran SD, Sit NW. Mode-dependent antiviral activity of medicinal plant extracts against the mosquito-borne Chikungunya virus. Plants. 2021;10(8):1658. https://doi.org/10.3390/plants10081658

Mao AA, Hynniewta TM, Sanjappa M. Plant wealth of Northeast India with reference to Ethnobotany. Indian J Tradit Knowl. 2009;8(1):96-03.

Samatha TA, Srinivas P, Shyamsundarachary RU, Rajinikanth M, Rama Swamy N. Phytochemical analysis of seeds, stem bark and root of an endangered medicinal forest tree Oroxylum indicum (L) Kurz. Int J Pharma Bio Sci. 2012;3(3):1063-75.

NandaTripathy B, Panda SK, Sahoo S, Mishra SK, Nayak L. Phytochemical examination and hepatoprotective impact of stem bark of Oroxylum indicum (L.) Vent. on carbon tetrachloride induced hepatotoxicity in rat. Technol Innov Pharm Res. 2021;12(3):113-20. https://doi.org/10.9734/bpi/tipr/v3/4069D

Essiett UA, Bassey IE. Comparative phytochemical screening and nutritional potentials of the flowers (petals) of Senna alata (L.) Roxb., Senna hirsuta (L.) Irwin and barneby, and Senna obtusifolia (l.) Irwin and Barneby (Fabaceae). Journal of Applied Pharmaceutical Science. 2013;3(8):97-101.

Rahman MA, Ahmed NU. Phytochemical and biological activities of ethanolic extract of C. hirsuta leaves. Bangladesh J Sci Ind Res. 2013;48(1):43-50. https://doi.org/10.3329/bjsir.v48i1.15413

Essien EE, Thomas PS, Ascrizzi R, Setzer WN, Flamini G. Senna occidentalis (L.) Link and Senna hirsuta (L.) HS Irwin and Barneby: constituents of fruit essential oils and antimicrobial activity. Nat Prod Res. 2019;33(11):1637-40. https://doi.org/10.1080/14786419.2018.1425842

Singh TT, Devi AR, Sharma HM. Ethnopharmacological survey of medicinal plants in Andro Village in Manipur (India). Res J Life Sci. 2018;4:606-23. https://doi.org/10.26479/2018.0405.49

Madankumar D, Pari S. Evaluation of in-vitro antidiabetic and antioxidant activity using methanolic extract of medicinal plants (Senna alata and Senna hirsuta). Solid State Technol. 2020;4962-73.

Akharaiyi FC, Boboye B, Akpambang VO. Antibacterial and biochemical effects of ethanol leaf extract of Senna hirsuta Mill using animal model-mice. J Microbiol Biotechnol Food Sci. 2021;4(4):292-96. https://doi.org/10.15414/jmbfs.2015.4.4.292-296

Barbosa-Ferreira M, Dagli ML, Maiorka PC, Górniak SL. Sub-acute intoxication by Senna occidentalis seeds in rats. Food Chem Toxicol. 2005;43(4):497-503. https://doi.org/10.1016/j.fct.2004.11.017

Olapade AA, Ajayi OA. Effect of roasting regime on phytochemical properties of Senna occidentalis seeds. Int. J Food Stud. 2016;5(2):203-11. https://doi.org/10.7455/ijfs/5.2.2016.a8

Nuhu A, Aliyu R. Effects of Cassia occidentalis aqueous leaf extract on biochemical markers of tissue damage in rats. Trop J Pharm Res. 2008;7(4):1137-42. https://doi.org/10.4314/tjpr.v7i4.14699

Daskum AM, Godly C, Qadeer MA, Ling LY. Effect of Senna occidentalis (Fabaceae) leaves extract on the formation of ?-hematin and evaluation of in vitro antimalarial activity. Int J Herb Med. 2019;7(3):46-51. https://doi.org/10.3390/plants10081658

Issa TO, Mohamed Ahmed AI, Mohamed YS, Yagi S, Makhawi AM, Khider TO. Physiochemical, insecticidal and antidiabetic activities of Senna occidentalis Linn. root. Biochem Res Int. 2020;2020:8810744. https://doi.org/10.1155/2020/8810744

Tsado NA, Lawal B, Kontagora GN, Muhammad BM, Yahaya MA, Gboke JA et al. Antioxidants and antimicrobial-activities of methanol leaf extract of Senna occidentalis. J Adv Med Pharm Sci. 2016;8(2)1-7. https://doi.org/10.9734/JAMPS/2016/25655

Daskum AM, Godly C, Qadeer MA, Ling LY. Effect of Senna occidentalis (Fabaceae) leaves extract on the formation of ?-hematin and evaluation of in vitro antimalarial activity. Int J Herb Med. 2019;7(3):46-51.

Deb PK, Ghosh R, Chakraverty R, Debnath R, Das L, Bhakta T. Phytochemical and pharmacological evaluation of fruits of Solanum indicum Linn. Int J Pharm Sci Rev Res. 2014;25(2):28-32.

Saxena HO, Pawar G. Total phenolic and caffeic acid contents in roots of Solanum indicum L. from different agroclimatic regions of Madhya Pradesh State of India. Indian J Pharm Educ Res. 2019;53(2S):s164-69. https://doi.org/10.5530/ijper.53.2s.62

Ghani. Medicinal Plants of Bangladesh: Chemical Constituents and Uses, 2nd Edition, Asiatic Society of Bangladesh, Dhaka, 2003, p. 1-16.

Shankar R, Neyaz S, Anku G, Rungsung LW. Exploration, conservation and cultivation of Stephania japonica (Thunb.) Miers. J Drug Res Ayurvedic Sci. 2020;5(3):143-44. https://doi.org/10.5005/jdras-10059-0098

Sultana MC, Alam MB, Asadujjaman M, Akter MA. Antihyperglycemic and antihyperlipidemic effects of Stephania japonica (Thunb.) Miers. Tenril in alloxan induced diabetic mice. Int J Pharm Sci Rev Res. 2012;3(8):2726-32.

Uddin SN, Amin MN, Shahid-Ud-Daula AF, Hossain H, Haque MM, Rahman MS et al. Phytochemical screening and study of antioxidant and analgesic potentials of ethanolic extract of Stephania japonica Linn. Journal of Medicinal Plants Research. 2014;8(37):1127-33. https://doi.org/10.5897/JMPR2014.5443

Tripathi R, Verma S, Easwari TS, Shah H. Standardization of some herbal anti-diabetic drugs in polyherbal formulation and their comparative study. Int J Pharm Sci Res. 2013;4(8):3256-65. http://dx.doi.org/10.13040/IJPSR.0975-8232.4(8).3256-65

Madhavi N, Kumar D, Naman S, Singh M, Singh PA, Bajwa N, Baldi A. Formulation and evaluation of novel herbal formulations incorporated with Amla extract for improved stability. Journal of Drug Delivery and Therapeutics. 2019;9(4):212-21.

Anilkumar V, Kalyani R, Padmasri, B, Prasanth, D. In-house preparation, development and evaluation of herbal cosmetics face pack using various natural powders. J Drug Deliv Sci Technol. 2020;10(5):159-64. https://doi.org/10.22270/jddt.v10i5.4314

Trease GE and Evans WC, 1989. Pharmacognosy. 11th Edn. Brailliar Tiridel Can. Machmillian publishers.

Goyal AK, Middha SK, Sen A. Evaluation of the DPPH radical scavenging activity, total phenols and antioxidant activities in Indian wild Bambusa vulgaris" Vittata" methanolic leaf extract. Journal of Natural Pharmaceuticals. 2010;1(1):40-45. https://doi.org/10.4103/2229-5119.73586

Ranjith D. Fluorescence analysis and extractive values of herbal formulations used for wound healing activity in animals. J Med Plants Stud. 2018;6(2):189-92.

Isah RT, Mohammed MO, Muhammad AT, Sahabi SM, Umar ZU, Mahmud RI et al. Effects of aqueous leaf extracts of Senna occidentalis on rat kidney. Afr J Biomed Res. 2018;21(2):225-30.

Baby S, Antony AV, John B, Jose D. Phytochemical screening and in-vitro anti-inflamatory activity of ethanolic extract of Centella asiatica. Int J Curr Med Pharm Res. 2020;2(2):181-83. https://doi.org/10.37022/WJCMPR.2020.2218

Rai B, Bhutia S, Pal P, Kakoti BB. Phytochemical analysis and antibacterial evaluation against selected gram strains by Oroxylum indicum (L.) Kurz stem bark extract, a folklore medicine of Sikkim Himalaya. J Pharmacogn Phytochem. 2020;9:11-16.

Windyaswari AS, Purba JP, Nurrahmah SS, Ayu IP, Imran Z, Amin AA, Kurniawan F, Pratiwi NT, Iswantari A. Phytochemical profile of sea grass extract (Enhalus acoroides): A new marine source from Ekas Bay, East Lombok. IOP Conf Ser Earth Environ Sci. 2019;278(1), 012081. IOP Publishing. https://doi.org/10.1088/1755-1315/278/1/012081

Tamasi A, Shoge MO, Adegboyega T, Chukwuma E. Phytochemical analysis and in-vitro antimicrobial screening of the leaf extract of Senna occidentalis (Fabaceae). Asian J Nat Prod Biochem. 2021;19(2):57-64. https://doi.org/10.13057/biofar/f190203

Kewatkar SM, Paithankar VV, Deshpande SS, Jain SP, Nagore DH, Bhusari DV et al. Comparative pharmacognostical and phytochemical study of Cassia auriculata and Cassia fistula. J Nat Remedies. 2021;21(1):77-85. https://doi.org/10.18311/jnr/2021/25626

Aslam I, Iqbal J, Peerzada S, Afridi MS, Ishtiaq S. Microscopic investigations and pharmacognostic techniques for the standardization of Caralluma edulis (Edgew.) Benth. ex Hook. f. Microsc Res Tech. 2019;82(11):1891-02. https://doi.org/10.1002/jemt.23357

Wang X, Shen C, Meng P, Tan G, Lv L. Analysis and review of trichomes in plants. BMC Plant Biol. 2021;21(70):1-11. https://doi.org/10.1186/s12870-021-02840-x

Bisht A, Zaman K, Singh M, Gupta R, Singh V. Pharmacognostical studies on Oroxylum indicum (Linn.) Vent. stem bark. Indian J Nat Prod Resour. 2011;2(4):472-78.

Sudhakaran MV. Botanical Pharmacognosy of Centella asiatica (Linn.) Urban. Pharmacognosy Journal. 2017;9(4):546-58. https://doi.org/10.5530/pj.2017.4.88

Dutta S, Das M, Banerji A, Rao MM. Pharmacognostical evaluation of Alstonia scholaris (L.) R. Br. (Saptapar?a), roots. Journal of Pharmacognosy and Phytochemistry. 2019;8(2):2096-101.

Kaur P, Kataria SK, Singh B, Arora S. Pharmacognostic Investigation of Punica granatum L. Peel. Int J Pharm Drug Anal. 2018;6(2):116-21.

Luo L, Wang B, Jiang J, Fitzgerald M, Huang Q, Yu Z, et al. Heavy metal contaminations in herbal medicines: Determination, comprehensive risk assessments and solutions. Frontiers Pharmacol. 2020;11: 595335. https://doi.org/10.3389/fphar.2020.595335

Alias N, Rosli SA, Sazalli NA, Hamid HA, Arivalakan S, Umar SN, et al. Metal oxide for heavy metal detection and removal. InMetal Oxide Powder Technologies. 2020;299-32. https://doi.org/10.1016/B978-0-12-817505-7.00015-4

Sharma AK, Kaushik K. Formulation and evaluation of herbal antidiabetic tablet. J drug Deliv Ther. 2011;1(1):65-67. https://doi.org/10.22270/jddt.v1i1.24

Attia UM, Fones A, Trepleton R, Hamilton H, Davies S, Wimpenny D. HIPing of Pd-doped titanium components: A study of mechanical and corrosion properties. In: Proceedings of the 11th International Conference on Hot Isostatic Pressing, Stockholm, Sweden. 2014. p. 9-13.

Published

09-05-2022 — Updated on 03-08-2022

Versions

How to Cite

1.
Mochahary B, Brahma S, Kalita M, Goyal AK. Characterisation of indigenous plants for herbal formulations preparation based on pharmacognostic and physiochemical data. Plant Sci. Today [Internet]. 2022 Aug. 3 [cited 2024 May 19];9(sp2):8-17. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/1709

Issue

Section

Research Articles