In vitro and in vivo evaluation of different measures to control Ascochyta blight in chickpea

Saima  Naseer; Yasir  Ali; Azher  Mustafa; Javed  Anwar shah; Saba  Saeed; Hafiz Muhammad  Aatif; Salman  Ahmad; Muhammad Jawwad  Yousaf; Abdul  Shakoor; Muhammad  Ramzan

doi:10.14719/pst.1781

Authors

Saima Naseer Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan https://orcid.org/0000-0003-1830-5876
Yasir Ali College of Agriculture Bahauddin Zakariya University, Bahadur Sub-Campus Layyah https://orcid.org/0000-0002-3795-7469
Azher Mustafa Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan https://orcid.org/0000-0002-3534-1943
Javed Anwar shah Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan https://orcid.org/0000-0001-8870-099X
Saba Saeed Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan https://orcid.org/0000-0003-4369-5546
Hafiz Muhammad Aatif Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan https://orcid.org/0000-0002-0883-9287
Salman Ahmad Department of Plant Pathology, University College of Agriculture, University of Sargodha-Pakistan https://orcid.org/0000-0002-5603-693X
Muhammad Jawwad Yousaf Plant Pathology Research Institute, Ayub Agriculture Research Institute (AARI), Faisalabad-Pakistan
Abdul Shakoor Department of Agronomy, University of Agriculture Faisalabad-Pakistan https://orcid.org/0000-0001-8674-4489
Muhammad Ramzan Deputy Director (Trg Ento), Plant Pathology Research Institute, Faisalabad-Pakistan https://orcid.org/0000-0001-6319-0460

DOI:

https://doi.org/10.14719/pst.1781

Keywords:

Ascochyta blight, Biocontrol, Chickpea, Fungicides, Plant extracts

Abstract

Ascochyta blight, an infection caused by Ascochyta rabiei is a destructive disease in many chickpea growing regions and it caused significant yield losses. To minimize the impact of Ascochyta blight, 5 fungicides viz., Aliette, Cabrio Top, Thiovit Jet, Cymoxanil and Difenoconazole, 5 plants extracts namely Azadirachta indica, Azadirachta azedarach, Datura stramonium, Chenopodium album and Allium sativum L. and two strains T-22 and E58 of bio-control agents (BCAs) Trichoderma viride and Aspergillus flavus were evaluated on the growth of A. rabiei under in vitro conditions by using the food poison technique. The colony growth of Ascochyta rabiei was inhibited at all concentrations of fungicides @ 0.07, 0.15, 0.21%, plants extracts @ 4, 6, 9% and bio-control agents @ 105, 106 and 107 conidia ml-1 respectively. Among all applied treatments, maximum inhibition colony growth of pathogen was recorded in the case of Aliette (83.4%), followed by Cabrio Top (74.3%), Azadirachta indica (50.3%) and Trichoderma viride (60.3%) at their high concentrations. Field trials showed that Aliette and Cabario Top significantly reduced the disease severity to 10 % and 24% respectively, followed by Azadirachta indica and Allium sativum which reduced the disease severity to 40% and 50% respectively. Bio-control agent Trichoderma viride proved less effective in controlling Ascochyta bight severity under field conditions. The present study showed that systemic and sulphur containing fungicides, plant extracts and bio-control agents (BCAs) have the potential to control Ascochyta blight in both in vitro and in vivo conditions.

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