Anti-bacterial potential of (Acacia nilotica, Trigonella foenum-graecum, Punica granatum and Commiphora myrrha) crude extracts against diverse drug sensitive and resistant bacterial species

Meaad  Fakhraldeen Abdalla Osman; Sababil Salih Abdalla; Sara  Abdelghani; Altaf Ali  Mangi; Lienda Bashier Eltayeb

doi:10.14719/pst.1874

Authors

Meaad Fakhraldeen Abdalla Osman Department of Microbiology, Faculty of Medical Laboratory Sciences National University-Khartoum, 11115, Sudan https://orcid.org/0000-0002-5806-4512
Sababil Salih Abdalla Department of Microbiology, Faculty of Medical Laboratory Sciences National University-Khartoum, 11115, Sudan https://orcid.org/0000-0002-0900-4265
Sara Abdelghani Department of Parasitology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, 11115, Sudan. https://orcid.org/0000-0002-9133-0392
Altaf Ali Mangi Faculty of Pharmacy Gomal University Dera Ismail Khan Khyberpakhunkhaw, 29111, Pakistan https://orcid.org/0000-0001-9358-7290
Lienda Bashier Eltayeb Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Alkharj, 16248, Saudi Arabia. https://orcid.org/0000-0002-9674-9133

DOI:

https://doi.org/10.14719/pst.1874

Keywords:

Plant Extract, Antimicrobial effects, Bacterial species, Multi-drug resistant

Abstract

The alarming increase in bacterial resistance to antibiotics caused some authors to state that we are approaching a post-antibiotic era and medical catastrophe, the study aimed to assess the antimicrobial effects of selected plant extracts against several sensitive and resistant bacterial isolates. Experimental cross-sectional study was conducted, 70% ethanol crude Acaccia nilotica, Trigonella foenum-graecum, Punica granatum and Commiphora myrrha extract was prepared and several commercial antimicrobials agent tested, the antibacterial activity was investigated using the disc diffusion method. The inhibition zones' diameters (mm) were calculated and interpreted by Zone Diameter Interpretative Standards. Data were analyzed by using (SPSS) software version 22. About 200% of A. nilotica and T. foenum-graecum showed bactericidal effects against Enterococcus faecalis, means ±SD (12.3 ± 2.8 and 12.5 ± 2.1). The activity of 200% C. myrrha extract was highest against all diverse bacterial. Despite a relatively high inhibition zone among all plant ethanol extracts, the findings demonstrate that there is no statistical significance in the inhibitory activity impact of varying concentrations of 70% ethanol extracts of all plants extract against bacterial isolates (P. value ? 0.05). The outcomes of the ethanol extracts of the used plant under study demonstrated that the herbal extract can be a superior antimicrobial potential than the result of the commercial broad spectrum antimicrobial agent utilized. C. myrrha extract was potent antimicrobial activity against all diverse bacterial species.

Downloads

Download data is not yet available.

References

Yadav UC, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharm Biol. 2014;52(2):243-254. https://doi.org/10.3109/13880209.2013.826247

Braga LC, Shupp JW, Cummings C, et al. Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol. 2005;96(1-2):335-339. https://doi.org/10.1016/j.jep.2004.08.034

Bhattacharjee MK, Alenezi T. Antibiotic in myrrh from Commiphora molmol preferentially kills nongrowing bacteria. Future Sci OA. 2020;6(4):FSO458. Published 2020 Feb 20. https://doi.org/10.2144/fsoa-2019-0121

Abreu AC, McBain AJ, Simões M. Plants as sources of new antimicrobials and resistance-modifying agents. Nat Prod Rep. 2012;29(9):1007-1021. https://doi.org/10.1039/c2np20035j

Hemaiswarya S, Kruthiventi AK, Doble M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine. 2008;15(8):639-652. doi:10.1016/j.phymed.2008.06.008

Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance. J Ayurveda Integr Med. 2017;8(4):266-275. https://doi.org/10.1016/j.jaim.2017.05.004

Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nat Rev Drug Discov. 2005;4(3):206-220. https://doi.org/10.1038/nrd1657

Harbottle H, Thakur S, Zhao S, White DG. Genetics of antimicrobial resistance. Anim Biotechnol. 2006;17(2):111-124. https://doi.org/10.1080/10495390600957092

Reygaert WC. An overview of the antimicrobial resistance mechanisms of bacteria. AIMS Microbiol. 2018;4(3):482-501. Published 2018 Jun 26. doi:10.3934/microbiol.2018.3.482.https://doi.org/10.3934/microbiol.2018.3.482

Pendleton JN, Gorman SP, Gilmore BF. Clinical relevance of the ESKAPE pathogens. Expert Rev Anti Infect Ther. 2013;11(3):297-308. doi:10.1586/eri.13.12. https://doi.org/10.1586/eri.13.12

Wu CJ, Lee HC, Lee NY, et al. Predominance of Gram-negative bacilli and increasing antimicrobial resistance in nosocomial bloodstream infections at a university hospital in southern Taiwan, 1996-2003. J Microbiol Immunol Infect. 2006;39(2):135-143.

Chen ML, Hao Z, Tian Y, Zhang QY, Gao PJ, Jin JL. Different Effects of Six Antibiotics and Ten Traditional Chinese Medicines on Shiga Toxin Expression by Escherichia coli O157:H7. Evid Based Complement Alternat Med. 2013;2013:121407. https://doi.org/10.1155/2013/121407

Savoia D. Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol. 2012;7(8):979-990. https://doi.org/10.2217/fmb.12.68

Termentzi A, Fokialakis N, Skaltsounis AL. Natural resins and bioactive natural products thereof as potential antimicrobial agents. Curr Pharm Des. 2011;17(13):1267-1290. https://doi.org/10.2174/138161211795703807

Sadiq MB, Tarning J, Aye Cho TZ, Anal AK. Antibacterial Activities and Possible Modes of Action of Acacia nilotica (L.) Del. against Multidrug-Resistant Escherichia coli and Salmonella. Molecules. 2017;22(1):47. Published 2017 Jan 14. https://doi.org/10.3390/molecules22010047

Brantner A, Pfeiffer KP, Brantner H. Applicability of diffusion methods required by the pharmacopoeias for testing antibacterial activity of natural compounds. Pharmazie. 1994;49(7):512-516.

Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH, Nisar MA, Alvi RF, Aslam MA, Qamar MU, Salamat MKF, Baloch Z. Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist. 2018 Oct 10;11:1645-1658. doi: 10.2147/IDR.S173867. PMID: 30349322; PMCID: PMC6188119.

Babiker EE, Al-Juhaimi FY, Alqah HA, et al. The effect of Acacia nilotica seed extract on the physicochemical, microbiological and oxidative stability of chicken patties. J Food Sci Technol. 2019;56(8):3910-3920. https://doi.org/10.1007/s13197-019-03862-y

Perumal Samy R, Gopalakrishnakone P. Therapeutic Potential of Plants as Anti-microbials for Drug Discovery. Evid Based Complement Alternat Med. 2010 Sep;7(3):283-94. doi: 10.1093/ecam/nen036. Epub 2008 Jun 24. PMID: 18955349; PMCID: PMC2887332.

Sadiq MB, Hanpithakpong W, Tarning J, Anal AK. Screening of phytochemicals and in vitro evaluation of antibacterial and antioxidant activities of leaves, pods and bark extracts of Acacia nilotica (L.) Del. Ind Crops Prod. 2015;77:873–882. https://doi.org/10.1016/j.indcrop.2015.09.067

Chalghoumi R, Mabrouki S, Abdouli H, Line JE. Antibacterial Activity of Fenugreek Seeds (Trigonella foenum-graecum) Crude Extracts Against a Rabbit Escherichia coli Isolate. Acad. J. Microbiol. Res. 2016; 4(11): 139-144. DOI: 10.15413/ajmr.2016.0121

Al-Timimi LAN. Antibacterial and Anticancer Activities of Fenugreek Seed Extract. Asian Pac J Cancer Prev. 2019 Dec 1;20(12):3771-3776. doi: 10.31557/APJCP.2019.20.12.3771. PMID: 31870120; PMCID: PMC7173369.

Shan B, Cai YZ, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol. 2007;117(1):112-119. https://doi.org/10.1016/j.ijfoodmicro.2007.03.003

Mahboubi A, Asgarpanah J, Sadaghiyani PN, Faizi M. Total phenolic and flavonoid content and antibacterial activity of Punica granatum L. var. pleniflora flowers (Golnar) against bacterial strains causing foodborne diseases. BMC Complement Altern Med. 2015;15:366. Published 2015 Oct 15. https://doi.org/10.1186/s12906-015-0887-x

Choi JG, Kang OH, Lee YS, et al. In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella. Evid Based Complement Alternat Med. 2011;2011:690518. https://doi.org/10.1093/ecam/nep105

Saquib SA, AlQahtani NA, Ahmad I, Kader MA, Al Shahrani SS, Asiri EA. Evaluation and Comparison of Antibacterial Efficacy of Herbal Extracts in Combination with Antibiotics on Periodontal pathobionts: An in vitro Microbiological Study. Antibiotics (Basel). 2019 Jul 1;8(3):89. doi: 10.3390/antibiotics8030089. PMID: 31266146; PMCID: PMC6783985.

Al-sieni AI. The antibacterial activity of traditionally used Salvadora persica L. (miswak) and Commiphora gileadensis (palsam) in Saudi Arabia. Afr J Tradit Complement Altern Med. 2013;11(1):23-27. Published 2013 Nov 2.

Natarajan D, Britto JS, Srinivasan K, Nagamurugan N, Mohanasundari C, Perumal G. Anti-bacterial activity of Euphorbia fusiformis- a rare medicinal herb. Journal of Ethnopharmacology. 2005;102:123–126. doi: 10.1016/j.jep.2005.04.023.