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Plant Science Today (PST)

Research Articles

Vol. 3 No. 3 (2016)

In vitro evaluation of antibiotic performances on Trichoderma harzianum and some crop infecting fungi

DOI
https://doi.org/10.14719/pst.2016.3.3.194
Submitted
10 February 2016
Published
06-07-2016

Abstract

An in vitro experiment was carried out to evaluate the effectiveness of antibiotics at different concentrations on growth and development of Trichoderma harzianum, Phytophthora infestans, Colletotrichum gloeosporioides, Corticium theae and Fusarium oxysporum found in tea plantation. Three samples viz. sample-1 (Validamycin 60% w/w) @ 45, 60 and 75 ppm, sample-2 (Hexaconazole 2.5% w/w+Validamycin 8.5% w/w) @ 55, 82.5 and 110 ppm and sample-3 (Streptomycin 9% w/w+Tetracyclin hydrochloride 1% w/w) @ 50, 75 and 100 ppm were tested. The result showed that Antibiotics have inhibitory effects on T. harzianum. Unsatisfactory performances in terms of per cent growth inhibition (<80) were recorded on crop infecting fungi. C. theae treated with sample-1 @ 75 ppm and C. gloeosporioides with sample-2 @ 110 ppm had shown maximum 25.50 and 54.19 per cent growth inhibition, respectively. The highest 70.53 per cent growth inhibition of C. theae was observed in sample-3 treated @ 100 ppm. Considering the findings it can be recommended not to use above antibiotics with their respective concentrations in plant agriculture for controlling diseases caused by the said fungi.

References

  1. Anonymous. 1994. The Agrochemicals Handbook. Royal Society of Chemistry Information Systems. Third Edition. Unwin Brothers Ltd., Surrey, England.
  2. Anonymous. 2006. Ag Streptomycin. Makhteshim Agan of North America. Inc. 4515 Falls of Neuse Road, Suite 300 Raleigh, NC 27609. EPA Reg. No. 66222-12.
  3. CDC. 2013.Antibiotic Resistance Threats in the United States. Atlanta, GA; 2013.P. 7, 36-7.
  4. EPA. 1988. US Environmental Protection Agency. Fact Sheet Number 186 Streptomycin. USEPA. Washington, DC.
  5. Gelband, H., M. M. Petrie, S. Pant, S. Gandra, J. Levinson, D. Barter, A. White and R. Laxminarayan. 2015. The State of the World’s Antibiotics 2015. Center for Disease Dynamics, Economics & Policy 2015. CDDEP: Washington, D.C.
  6. Klajn, R. 2001. Tetracycline: Chemistry and Chemical Biology of Tetracyclines. Accessed from www.chm.bris.ac.uk/motm/tetracycline/tetracycline.htm
  7. Laar, W. V. and W. Gams. 1982. The use of Solacol (validamycin) as a growth retardant in the isolation of soil fungi. Netherlands Journal of Plant Pathology, Volume 88, Issue 2, pp 39-45.
  8. Maeda, Y., A. Kiba, K. Ohnishi and Y. Hikichi. 2004. Implications of amino acid substitutions in GyrA at position 83 in terms of oxolinic acid resistance in field isolates of Burkholderia glumae, a causal agent of bacterial seedling rot and grain rot of rice. Appl. environ. Microbiol., 70, 5613–5620. doi: 10.1128/AEM.70.9.5613-5620.2004
  9. McManus, P. S., V. O. Stockwell, G. W. Sundin and A. L. Jones. 2002. Antibiotic use in plant agriculture. Annu. Rev. Phytopathol., 40, 443–465. doi: 10.1146/annurev.phyto.40.120301.093927
  10. Meister, R. T. 1994. Farm Chemicals Handbook '94. Meister Publishing Company. Willoughby, OH.
  11. Nandakumar, R., A. K. M. Shahjahan, X. L. Yuan, E. R. Dickstein, D. E. Groth, C. A. Clark, R. D. Cartwright and M. C. Rush. 2009. Burkholderiaglumae and B. gladioli cause bacterial panicle blight in rice in the southern United States. Plant Dis., 93, 896–905. doi: 10.1094/PDIS-93-9-0896
  12. NIOSH. 1993. National Institute for Occupational Safety and Health. Registry of Toxic Effects of Chemical Substances (RTECS). NIOSH. Cincinnati, OH.
  13. Shtienberg, D., M. Zilberstaine, D. Oppenheim, Z. Herzog, S. Manulis, H. Shwartz and G. Kritzman. 2001. Efficacy of oxolinic acid and other bactericides in suppression of Erwinia amylovora in pear orchards in Israel. Phytoparasitica, 29, 143–154. doi: 10.1007/BF02983958
  14. Stockwell, V. O. and B. Duffy. 2012. Use of antibiotics in plant agriculture, Rev. sci. tech. Off. int. Epiz., 31 (1), 199-210.VII.
  15. Thomson, W. T. 1982. Agricultural Chemicals Book IV Fungicides. Thomson Publications. Fresno, CA.
  16. Tomlin, C. D. S. 2000. The Pesticide Manual. British Crop protection Council, Twelfth Edition. 49 Downing Street, Farnham, Surrey GU9 7PH, UK.
  17. Vidaver, A. K. 2002. Uses of antibiotics in plant agriculture. Clin.infect. Dis., 34, S107–110. doi: 10.1086/340247
  18. Waksman, S. A. 1947. What Is an Antibiotic or an Antibiotic Substance? Mycologia 39 (5): 565–569. doi: 10.2307/3755196. JSTOR 3755196. PMID 20264541.
  19. Wikipedia. 2016. https://en.wikipedia.org/wiki/Penicillin
  20. Wikipedia. 2016. https://en.wikipedia.org/wiki/Streptomycin

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