Physiological alterations of Salvinia natans L. exposed to aluminium stress and its interaction with polyamine
DOI:
https://doi.org/10.14719/pst.2016.3.2.198Keywords:
Al toxicity, western blotting, gene expression, HPLC, polyamine, Salvinia natansAbstract
In the present Investigation Salvinia natans L exposed to increasing concentration of Al stress and its interaction with polyamine was discussed. Among the physiological attributes Glutathione-S-transferase, glutathione peroxidase activities were up regulated against control. At transcript level glutathione peroxidase was also shown by over-expressed manner to support the lyses of peroxide. However, the application of Put had minimized these activities with same way to establish the role of polyamine under metal stress. In secondary metabolites synthetic pathway phenyl alanine ammonia lyase recorded a steady increase although the concentration of Al. Not only a single fraction of polyamine was responsible under Al stress but also pool of conjugated polyamine was up regulated. In oxidation of polyamine the activity of diamine oxidase (DAO) was more under metal stress to induce accumulation H2O2. In compensation for cellular depletion of reduced glutathione, dihydro ascorbate reductase activity was up regulated in plant under stress. At cellular level plants were distinctly marked with variations in heat shock proteins and established as a possible biomarker for Al toxicity. The study possibly established the affectivity in bio-monitoring of Al in field condition with exercise cellular responses of Salvinia plants.Downloads
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