Identification and authentication of Agnimantha plant species used in Ayurveda on the basis of anatomical and molecular phylogenetic analysis
DOI:
https://doi.org/10.14719/pst.2180Keywords:
Anatomy, Molecular phylogeny, Ayurveda, AgnimanthaAbstract
Agnimantha plant species have been used in the Ayurvedic system of medicine for many years and is widely used as an ingredient in many ayurvedic formulations. However, the source for Agnimantha remained controversial as it is difficult to authenticate from various reports. Hence, the present study aims to identify and authenticate its original and substitute sources. As per the literature sources Clerodendrum phlomidis L.f., C. inerme (L.) Gaertn. and Premna serratifolia L. are considered Agnimantha species. The anatomy of the above mentioned species confirmed the presence of patches of up to 20 cells in the sclerenchyma of the root cortex, while in the absence of sclerenchyma of the stem cortex, abundant chambered crystals were also present in the bark of the stem and root in C. phlomidis as compared to C. inerme and P. serratifolia. Phylogenetic analysis using chloroplast (matK, trnH-psbA) and nuclear markers (ITS, rbcl) also indicates the close relation between C. inerme and P. serratifolia and hence places them both in the same clade, though C. phlomidis is closely related to the other species but placed in the adjacent clade. Hence, the study concludes that anatomical as well as molecular phylogenetic analysis reflect close relation between C. inerme and P. serratifolia. while a distant relation with C. phlomidis.
Downloads
References
Kamat SD, Kamat DK. Studies on medicinal plants and drugs in Dhanvantari-Nighantu.
Raja Nighantu, Indradeeva Tripathi. Choukhambha orientalia, Varanasi, Prabhadradi varga. 2003;pp.268.
Anonymous. The Ayurvedic Pharmacopoeia, Part 1, 1st edn, Vol. III, (Ministry of Health and Family Welfare, Department of Indian Systems of Medicine and Homoeopathy, Government of India). 2000;3(29):,147,178,209.
Vaidya BG. Nighantu adarsha. Chaukhambha Vidyabhavan. 1968;551-54.
Ved DK, Goraya GS. Demand and supply of medicinal plants. Medplant-ENVIS Newsletter on Medicinal Plants. 2008 Dec;1(1):2-4.
Razafiniary, V. Premna serratifolia. The IUCN Red List of Threatened Species. 2021: e.T66295794A68121996. https://dx.doi.org/10.2305/IUCN.UK.2021-1.RLTS.T66295794A68121996.en. Accessed on 17 July 2023.
Agnivesha C Dridhabala, Charaka samhita Chikitsha Sthana, Prameha Chikitshadhyaya. 2001;6(47-48):2.
Agarwal VS. Rural economics of medicinal plants, vegetation in the forests. In: Drug plants of India. Kalyani Publishers, New Delhi. 1997;1(6):44.
Khare CP. Indian medicinal plants: an illustrated dictionary. Springer Science and Business Media. 2008 Apr 22; https://doi.org/10.1007/978-0-387-70638-2
Anonymous: Wealth of India. Council of Scientific and Industrial Research. 1948-76;2:67.
Kumar D, Kumar A, Prakash O. Pharmacognostic investigation of Clerodendrum phlomidis Linn. f. root. J Trop Life Sci. 2014 Jun 2;4(2):96-100. https://doi.org/10.11594/jtls.04.02.03
Sharma M, Raval N, Dave A, Shukla VD. Pharmacognostical evaluation of Clerodendrum Serratum (Linn.) Moon, (Bharangi) Root. Indian J Ancient Med Yoga. 2013 Jul 1;6(3):95.
Kumari HA, Shrikanth P, Pushpan RE, Harisha CR, Nishteswar K. Comparative pharmacognostical evaluation of leaves of regionally accepted source plants of Agnimantha. J Res Educ Indian Med. 2013;19(1-2):1-8.
Susan RS, Harini A, KN SK, Hegde PL. Pharmacognostic and phytochemical analysis of Agnimantha (Premna corymbosa Rottl.) leaf. J Ayurv Herb Med. 2016;1(2). https://doi.org/10.5530/jams.2016.1.14
Yang Z, Rannala B. Molecular phylogenetics: principles and practice. Nature Rev Genet. 2012 May;13(5):303-14. https://doi.org/10.1038/nrg3186
Group CP, Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham S, van der Bank M, Chase MW, Cowan RS, Erickson DL, Fazekas AJ. A DNA barcode for land plants. Proc Nation Acad Sci. 2009 Aug 4;106(31):12794-97. https://doi.org/10.1073/pnas.0905845106
Li P, Qi ZC, Liu LX, Ohi-Toma T, Lee J, Hsieh TH, Fu CX, Cameron KM, Qiu YX. Molecular phylogenetics and biogeography of the mint tribe Elsholtzieae (Nepetoideae, Lamiaceae), with an emphasis on its diversification in East Asia. Scientific Reports. 2017 May 17;7(1):1-2. https://doi.org/10.1038/s41598-017-02157-6
Salmaki Y, Zarre S, Ryding O, Lindqvist C, Bräuchler C, Heubl G, Barber J, Bendiksby M. Molecular phylogeny of tribe Stachydeae (Lamiaceae subfamily Lamioideae). Mol Phylogenet Evol. 2013 Dec 1;69(3):535-51. https://doi.org/10.1016/j.ympev.2013.07.024
Scheen AC, Bendiksby M, Ryding O, Mathiesen C, Albert VA, Lindqvist C. Molecular phylogenetics, character evolution and suprageneric classification of Lamioideae (Lamiaceae) 1. Ann Missouri Bot Gard. 2010 Jul;97(2):191-217. https://doi.org/10.3417/2007174
Pham MP, Tran VH, Vu DD, Nguyen QK, Shah SN. Phylogenetics of native conifer species in Vietnam based on two chloroplast gene regions rbcL and matK. Czech J Genet Plant Breed. 2021 Apr 13;57(2):58-66. https://doi.org/10.17221/88/2020-CJGPB
Wattoo JI, Saleem MZ, Shahzad MS, Arif A, Hameed A, Saleem MA. DNA barcoding: amplification and sequence analysis of rbcl and matK genome regions in three divergent plant species. Advancements in Life Sciences. 2016 Nov 25;4(1):03-07.
Johansen D. A plant microtechnique, London: Mc Graw-Hill Book Company, Inc. 1940.
Veldman S, Ju Y, Otieno JN, Abihudi S, Posthouwer C, Gravendeel B, van Andel TR, de Boer HJ. DNA barcoding augments conventional methods for identification of medicinal plant species traded at Tanzanian markets. J Ethnopharmacol. 2020 Mar 25;250:112495. https://doi.org/10.1016/j.jep.2019.112495
Gogoi B, Wann SB, Saikia SP. DNA barcodes for delineating Clerodendrum species of North East India. Scientific Reports. 2020 Aug 10;10(1):13490. https://doi.org/10.1038/s41598-020-70405-3
Kress WJ. Plant DNA barcodes: Applications today and in the future. J Syst Evol. 2017 Jul;55(4):291-307. https://doi.org/10.1111/jse.12254
Li X, Yang Y, Henry RJ, Rossetto M, Wang Y, Chen S. Plant DNA barcoding: from gene to genome. Biol Rev. 2015 Feb;90(1):157-66. https://doi.org/10.1111/brv.12104
Vertuani S, Beghelli E, Scalambra E, Malisardi G, Copetti S, Toso RD, Baldisserotto A, Manfredini S. Activity and stability studies of verbascoside, a novel antioxidant, in dermo-cosmetic and pharmaceutical topical formulations. Molecules. 2011 Aug;16(8):7068-80. https://doi.org/10.3390/molecules16087068
Steane DA, Scotland RW, Mabberley DJ, Wagstaff SJ, Reeves PA, Olmstead RG. Phylogenetic relationships of Clerodendrum s.l. (Lamiaceae) inferred from chloroplast DNA. Syst Bot. 1997 Apr 1;229-43. https://doi.org/10.2307/2419455
Steane DA, Scotland RW, Mabberley DJ, Olmstead RG. Molecular systematics of Clerodendrum (Lamiaceae): ITS sequences and total evidence. Amer J Bot. 1999 Jan;86(1):98-107. https://doi.org/10.2307/2656958
Raj KA, Manimohan P. A new species and a new record of Clitopilus and a description of C. orientalis from India based on morphology and molecular phylogeny. Phytotaxa. 2018 Mar 6;343(1):47-59. https://doi.org/10.11646/phytotaxa.343.1.4
Ravikumar K, Ravichandran P. Taxonomy, pharmacognosy and phytochemical characteristics to identify the authentic and substitute botanical sources used as Agnimantha. Indian J Tradit Knowl. (IJTK). 2021 Apr 6;20(2):351-57. https://doi.org/10.56042/ijtk.v20i2.27806
Bansal S, Thakur S, Mangal M, Mangal AK, Gupta RK. DNA barcoding for specific and sensitive detection of Cuminum cyminum adulteration in Bunium persicum. Phytomedicine. 2018 Nov 15;50:178-83. https://doi.org/10.1016/j.phymed.2018.04.023
Issaravanich S, Ruangrungsia N, Palanuvej C, Vipunngeun N, Rungsihirunrat K. Microscopic, molecular and scopolamine content evaluations of Datura metel L. var. metel and D. metel L. var. fastuosa in Thailand. Res J of Pharmac Biolo Chem Sci. 2013;4:1009-21.
Sudmoon R, Chaveerach A, Tanee T. Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species. Pakistan J Pharmac Sci. 2016 Jan 1;29(1):65-76.
Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH. Use of DNA barcodes to identify flowering plants. Proc Nation Acad Sci. 2005 Jun 7;102(23):8369-74. https://doi.org/10.1073/pnas.0503123102
Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molec Biol Evol. 1993 May 1;10(3):512-26.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Molec Biol Evol. 2013 Dec 1;30(12):2725-29. https://doi.org/10.1093/molbev/mst197
Downloads
Published
Versions
- 01-10-2023 (2)
- 21-08-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Anurag Kumar, Prem Prakash Jangid, Srinivasan Marimuthu, Arun Manohar Gurav, N Srikanth, Anupam K Mangal, B Venkateshwarlu, N Shiddamallayya
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).