Production of Boeravinone-B, total phenolic, flavonoid content and antioxidant activity from callus cultures of Punarnava (Boerhavia diffusa L.)
DOI:
https://doi.org/10.14719/pst.2212Keywords:
Boerhavia diffusa, Punarnava, Boeravinone B, Rotenoid, Callus cultures, HPLCAbstract
Boerhavia diffusa L. (Punarnava) is a medicinal herb, rich in diversified plant secondary metabolites used in curing various health ailments. Boeravinone-B is one of the important phytochemicals reported in Punarnava, possessing various pharmacological activities. It belongs to the family of rotenoids, belonging to the isoflavone group. Production of Boeravinone-B from the Punarnava through conventional propagation is comparatively very low, and alternative interventions are of utmost importance to meet the growing demand. In view of this, the present study aims to develop biotechnological approaches like cell/tissue culture as a substitute strategy for the accumulation of biomass and Boeravinone-B biosynthesis. Callus was established from leaf explants of Boerhavia diffusa L. when cultured on MS semi solid medium fortified with varied concentrations and combinations of auxins and cytokinins. The callus induced on Murashige and Skoog medium (MS medium) supplemented with 5.0 ppm 2,4-Dichlorophenoxyacetic acid (2,4-D) favored the highest production of Boeravinone-B analyzed through High-performance Liquid chromatography (HPLC) and it was found to be 673.95 ?g g-1 Dry weight (DW). The total phenolic and flavonoid content were determined for the callus extracts and the results showed that callus induced on 5.0 ppm 2,4-D medium showed the highest phenolic and flavonoid content, which was 63.48 mg g-1 Gallic acid equivalent (GAE) Dry weight (DW), and 30.22 mg g-1 Quercetin equivalent (QE) DW. Similarly, antioxidant activities (radical scavenging, metal chelating, and reducing power) were performed, and it was found that callus induced on 5.0 ppm 2,4-D showed the highest anti-oxidant potential. Radical scavenging activity was found to be 91.1%, and 74% of metal chelating activity was recorded, and a similar trend was observed with respect to reducing power as well. The results of the present study lay foundation for optimization and subsequent large-scale production of Boeravinone-B from callus/cell suspension cultures.
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Mishra S, Aeri V, Gaur PK, Jachak SM. Phytochemical, therapeutic, and ethnopharmacological overview for a traditionally important herb: Boerhavia diffusa Linn. Biomed Res Int 2014; 808302. https://doi.org/10.1155/2014/808302
Kaur H.Boerhaavia diffusa: Bioactive compounds and pharmacological activities. Biomed Pharmacol J 2019; 12:1675–1682. https://doi.org/10.13005/bpj/1797
Kadota S, Lami N, Tezuka Y, Kikuchi T. Boeravinone A and B, new rotenoid analogues from Boerhaavia diffusa Linn. Chem Pharm Bull 1988; 36:834–836. https://doi.org/10.1248/cpb.36.834
Bairwa K, Singh IN, Roy SK, Grover J, Srivastava A, Jachak SM. Rotenoids from Boerhaavia diffusa as potential anti-inflammatory agents. J Nat Prod 2013; 76:1393–1398. https://doi.org/10.1021/np300899w
Bairwa K, Jachak SM. Anti-inflammatory potential of a lipid-based formulation of a rotenoid-rich fraction prepared from Boerhavia diffusa. Pharm Biol 2015; 53:1231–1238. https://doi.org/10.3109/13880209.2014.971382
Prathapan A, Varghese MV, Abhilash S, Mathew AK, Nair A, Nair RH, Raghu KG. Polyphenol rich ethanolic extract from Boerhavia diffusa L. mitigates angiotensin II induced cardiac hypertrophy and fibrosis in rats. Biomed Pharmacother 2017; 87:427-436. https://doi.org/10.1016/j.biopha.2016.12.114
Prathapan A, Raghu KG. Apoptosis in angiotensin II-stimulated hypertrophic cardiac cells-modulation by phenolics rich extract of Boerhavia diffusa L. Biomed Pharmacother 2018; 108:1097-1104.https://doi.org/10.1016/j.biopha.2018.09.114
Chen Y, Peng L, Shi S, Guo G, Wen H. Boeravinone B alleviates gut dysbiosis during myocardial infarction-induced cardiotoxicity in rats. J Cell Mol Med 2021; 25:6402-16. https://doi.org/10.1111/jcmm.16620
Huang Y, Sun Y, Wang W-W, Zhang L. Boeravinone B: a natural rotenoid exerts anticancer activity via inducing internalization and degradation of inactivated EGFR and ErbB2 in human colon cancer cells. Am J Transl Res 2018; 10:4183–4192.PMID: 30662661.
Singh S, Kalia NP, Joshi P, Kumar A, Sharma PR, Kumar A, Bharate SB, Khan IA. Boeravinone B, A novel dual inhibitor of NorA bacterial efflux pump of and human -glycoprotein, reduces the biofilm formation and intracellular invasion of bacteria. Front Microbiol 2017; 8:1868. https://doi.org/10.3389/fmicb.2017.01868
Krishnamoorthy PP, Muthukumaran S. In vitro studies to determine the effect of boeravinone B on human dendritic cells. Pharmacog Mag 2018; 14:465. https://doi.org/10.4103/pm.pm_625_17
Zhang J, Zong L, Bai D. Boeravinone B promotes fracture healing in ovariectomy induced osteoporotic rats via the regulation of NF-?B p65/I?B-?/SIRT-1 signaling pathway. Trop J Pharm Res 2021; 18:955–960. https://doi.org/10.4314/tjpr.v18i5.7
Rathor L, Pandey R. Age-induced diminution of free radicals by Boeravinone B in Caenorhabditis elegans. Exp Gerontol 2018;111:94–106. https://doi.org/10.1016/j.exger.2018.07.005
Jain V, Singh D, Saraf S, Saraf S. In-vitro culture studies for callus and root generation of Boerhaavia diffusa Linn. Anc Sci Life 2003; 22:134–139. PMID: 22557100
Sahu PR, Khalkho AS. Callus induction and in vitro multiplication of Boerhaavia diffusa–milestone medicinal plant of Jharkhand. Biogeosciences 2012; 7:123-127.
Patil KS, Bhalsing SR. Effect of sugars on production of ?-sitosterol from in vitro callus culture of Boerhaavia diffusa L. Acta Biol Szeged 2016; 60(2):99-104.
Sharma S, Sharma J, Singh D, Sharma V, Mallubhotla S. Effect of signal molecules for enhanced production of boeravinone B in shoot cultures of Boerhaavia diffusa (L.). Emir J Food Agric 2021; 33(10): 893 – 898. https://doi.org/10.9755/ejfa.2021.v33.i10.2778
Krishnamoorthy PK, Muthukumaran S, Maheswaran A, Sukumaran P. Isolation, purification and characterization of Boeravinone B from Boerhaavia diffusa Linn. Int Res J Pharm. 2017;8:140-144. https://doi.org/10.7897/2230-8407.0811233
Sembiring EN, Elya B, Sauriasari R. Phytochemical screening, total flavonoid and total phenolic content and antioxidant activity of different parts of Caesalpinia bonduc (L.) Roxb. Pharmacog J 2017; 10:123–127. https://doi.org/10.5530/pj.2018.1.22
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181:1199–1200. https://doi.org/10.1038/1811199a0
Chew YL, Goh JK, Lim YY. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food Chem 2009; 116(1):13-18. https://doi.org/10.1016/j.foodchem.2009.01.091
Chung I-M, Ali M, Praveen N, Yu BR, Kim SH, Ahmad A. New polyglucopyranosyl and polyarabinopyranosyl of fatty acid derivatives from the fruits of Lycium chinense and its antioxidant activity. Food Chem 2014; 151:435–443. https://doi.org/10.1016/j.foodchem.2013.11.061
Kanfade H, Rudra J, Bhowal M, Upadhyay A. In-vitro callus induction and shoot regeneration in Boerhaavia diffusa L. Ann Biol Res 2011; 2(1):142-148.
Ling AP, Tang KY, Gansau JA, Hussein S. Induction and maintenance of callus from leaf explants of Mirabilis jalapa L. Med Aromat Plant Sci Biotechnol 2009; 3(1):42-47.
Tahir SM, Victor K, Abdulkadir S. The effect of 2, 4-Dichlorophenoxy acetic acid (2, 4-D) concentration on callus induction in sugarcane (Saccharum officinarum). Nigerian J Basic Appl Sci 2011; 19(2):213-217.ISSN 0794-5698.
Ran S, Jessica JJ, Vickneswaran M, Sreeramanan S. Preliminary responses of 2, 4-D and BAP on callus initiation of an important medicinal-ornamental Hymenocallis littoralis plants. J Med Plant Res 2012; 6(11):2088-93. https://doi.org/10.5897/JMPR11.790
Chen DF, Li X, Xu Z, Liu X, Du SH, Li H, Zhou JH, Zeng HP, Hua ZC. Hexadecanoic acid from BuzhongYiqi decoction induced proliferation of bone marrow mesenchymal stem cells. J Med food 2010;13(4):967-75. https://doi.org/10.1089/jmf.2009.1293
Babu S, Jayaraman S. An update on ?-sitosterol: A potential herbal nutraceutical for diabetic management. Biomed Pharmacother 2020; 131:110702. https://doi.org/10.1016/j.biopha.2020.110702
Bakrim S, Benkhaira N, Bourais I, Benali T, Lee LH, El Omari N, Sheikh RA, Goh KW, Ming LC, Bouyahya A. Health Benefits and Pharmacological Properties of Stigmasterol. Antioxidants 2022;11(10):1912. https://doi.org/10.3390/antiox11101912
Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules 2009;14(1):540-54. https://doi.org/10.3390/molecules14010540
Gupta P, Sharma S, Saxena S. Biomass yield and steviol glycoside production in callus and suspension culture of Stevia rebaudiana treated with proline and polyethylene glycol. Appl Biochem Biotechnol 2015; 176: 863–874. https://doi.org/10.1007/s12010-015-1616-0
Kapoor S, Raghuvanshi R, Bhardwaj P, Sood H, Saxena S, Chaurasia OP. Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew. J Photochem Photobiol B: Biology 2018; 183:258-265. https://doi.org/10.1016/j.jphotobiol.2018.04.018
Fazal H, Abbasi BH, Ahmad N, Ali SS, Akbar F, Kanwal F. Correlation of different spectral lights with biomass accumulation and production of antioxidant secondary metabolites in callus cultures of medicinally important Prunella vulgaris L. J Photochem Photobiol B: Biology 2016;159:1-7. https://doi.org/10.1016/j.jphotobiol.2016.03.008
Parale A, Barmukh R, Nikam T. Influence of organic supplements on production of shoot and callus biomass and accumulation of bacoside in Bacopa monniera (L.) Pennell. Physiol Mol Biol Plants 2010; 16(2):167-175. https://doi.org/10.1007/s12298-010-0018-6
Bahorun T, Aumjaud E, Ramphul H, Rycha M, Luximon-Ramma A, Trotin F, Aruoma OI. Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung 2003; 47:191–198. https://doi.org/10.1002/food.200390045
Kumar MS, Chaudhury S, Balachandran S. In vitro callus culture of Heliotropium indicum Linn. for assessment of total phenolic and flavonoid content and antioxidant activity. Appl Biochem Biotechnol 2014; 174:2897–2909. https://doi.org/10.1007/s12010-014-1235-1
Christine S, Arvind B, Hag MD, Chan LK, Boey PL. Evaluation of free radical scavenging activity and total phenolic content in the petiole-derived callus cultures of Zingiber zerumbet Smith. J Med Plant Res 2011; 5(11):2210-2217.ISSN 1996-0875.
Ali AM, ElNour ME. Antioxidant activity, total phenolic, flavonoid and tannin contents of callus and seeds extracts of fenugreek (Trigonella foenum-graecum L.). Int J Sci Res2014; 3(10):1268-72. ISSN: 2319-7064
Jamwal K, Bhattacharya S, Puri S. Plant growth regulator mediated consequences of secondary metabolites in medicinal plants. J. Appl. Res. Med. Aromat. Plants 2018; 9:26-38. https://doi.org/10.1016/j.jarmap.2017.12.003.
Tadhani MB, Patel VH, Subhash R. In vitro antioxidant activities of Stevia rebaudiana leaves and callus. J Food compos Anal 2007; 20(3-4):323-329. https://doi.org/10.1016/j.jfca.2006.08.004
Koufan M, Belkoura I, Mazri MA, Amarraque A, Essatte A, Elhorri H, Zaddoug F, Alaoui T. Determination of antioxidant activity, total phenolics and fatty acids in essential oils and other extracts from callus culture, seeds and leaves of Argania spinosa (L.) Skeels. Plant Cell Tiss Organ Cult 2020; 141:217-27.
Park DE, Adhikari D, Pangeni R, Panthi VK, Kim HJ, Park JW. Preparation and characterization of callus extract from Pyrus pyrifolia and investigation of its effects on skin regeneration. Cosmetics 2018; 5:71. https://doi.org/10.3390/cosmetics5040071
Hakkim FL, Shankar CG, Girija S. Chemical composition and antioxidant property of holy basil (Ocimum sanctum L.) leaves, stems, and inflorescence and their in vitro callus cultures. J Agric Food Chem 2007; 55:9109–9117. https://doi.org/10.1021/jf071509h
Song H, Kumar P, Arivazhagan G, Lee SI, Yoon HM, Kim IH, Kwon HJ, Kim JM, Hakkim FL. Antioxidant property of leaves and calluses extracts of in-vitro grown 5 different Ocimum species. Plant Biotechnol J 2012; 39(3):146-53. https://doi.org/10.5010/JPB.2012.39.3.146
Giri L, Dhyani P, Rawat S, Bhatt ID, Nandi SK, Rawal RS, Pande V. In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: A rare Himalayan medicinal orchid. Ind Crops Prod 2012; 39:1–6. https://doi.org/10.1016/j.indcrop.2012.01.024
Gr?bkowska R, Matkowski A, Grzegorczyk-Karolak I, Wysoki?ska H. Callus cultures of Harpagophytum procumbens (Burch.) DC. ex Meisn.; production of secondary metabolites and antioxidant activity. S Afr J Bot 2016; 103:41–48. https://doi.org/10.1016/j.sajb.2015.08.012
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