Inhibitory effect of Polyram DF and Capsicum annum on leaf spot of rose caused by Curvularia lunata in vitro and in planta

Authors

DOI:

https://doi.org/10.14719/pst.2228

Keywords:

Cabrio top, Curzate-M, Aloe Vera, Mentha, Poisoned food technique

Abstract

Rose plants are affected by several diseases caused by fungi, nematode, bacteria, viruses, and other pests. Among all of these, Curvularia lunata causes significant losses to Roses. Present study was focused on In-vitro and In-vivo management of the “Curvularia leaf spot of Rose” caused by Curvularia lunata by using different fungicides and phyto-extracts. Diseased samples were collected from floriculture area of University of Agriculture, Faisalabad for isolation of pathogen. Five fungicides i.e., Cabrio-Top, Curzate-M, Aliette, Polyram-DF and Recado @ (50ppm, 100ppm and 150ppm) and five plant extracts i.e., Allium cepa, Capsicum annuum, Aloe vera, Menthaand Calotropis gigantean with three concentrations @ (5%, 10% and 15%) were evaluated under lab conditions through poisoned food technique by using Complete Randomized Design (CRD), where C. annuum gave best results (9.129mm) followed by Calotropis gigantea (13.003mm), and Polyram-DF was effective (2.218mm) followed by Curzate-M (6.542mm). Best performing fungicides and plant-extracts were subjected to In-vivo management trials. Under green-house conditions, combination of Capsicum annuum + Calotropis gigantean and Polyram-DF + Curzate-M were shown least disease incidence (14.517 and 3.224%). LSD was used for comparing variations between treatments at 5% probability. The results of these experiments were to aid in the evaluation of fungicides and Phyto-extracts, which are the most effective chemicals and phyto-extracts against leaf Spot disease of Rose.

Downloads

Download data is not yet available.

References

Desta B, Tena N, Amare G. Response of Rose (Rosa hybrida L.) Plant to Temperature. Asian Journal of Plant and Soil Sciences.2022:7(1):93-101.

Marriott, M. 2017. Modern (Post-1800). Reference Module in Life Sciences. doi.org/10.1016/B978-0-12

Gudin, S. Seed Propagation. Reference Module in Life Sciences. Elsevier. 2017 https://doi.org/10.1016/B978-0-12-809633-8.05093-7

Leghari AJ, Laghari UA, Laghari AH. Cultivation of rose (Rosa indica L.). Journal of Floriculture and Landscaping.2016; 2:1-4. doi:10.25081/jfcls.2016.v2.3044

Bhagat AA, Badgujar CD, Bhosale SS, Supe VS. An economics analysis for export of fresh cut rose flowers from India. Journal of Pharmacognosy and Phytochemistry.2019; 2: 291-298.

Afzal Y, Ali S, Ahmad, M, Chaudhry A. A Content Analysis of Masters Dissertations Submitted to The Department of Agricultural Extension, University of Agriculture, Faisalabad, Pakistan. Journal of Agricultural Research.2018; (03681157), 56(4):277-283.

Panwar R, Chandel S, Rani S. To study the effect of media on growth of the Marssonia rosae. Journal of Pharmacognosy and Phytochemistry.2019;8(1S):278-280.

Ghosh A, Shamsi S. Fungal diseases of rose plant in Bangladesh. Journal of Bangladesh Academy of Sciences.2014; 38(2):225-233 https://doi.org/10.3329/jbas.v38i2.21347

Dos Santos PR, de Souza Carlos Mourão D, Dalcin MS, Osorio PR, de Oliveira Lima FS, dos Santos GR. Pathogenicity of fungi associated with Andropogon grass seeds. Journal of Plant Pathology. 2022 May;104(2):565-73. https://doi.org/10.1007/s42161-022-01038-w

Liu Z, Liu T, Chen D, Hou J. First report of Curvularia lunata causing leaf spots on Partridge tea [Mallotus oblongifolius (Miq.) Mûll. Arg.] in China. Journal of Plant Patholog.2019;101(2):439-439.https://doi.org/10.1007/s42161-018-00208-z

Elkhateeb WA, Kolaibe A, Daba G.M. Cochliobolus,Drechslera Bipolaris, Curvularia different nomenclature for one potent fungus. Journal of Pharmaceutics and Pharmacology Research.2021;4(1)1-6. DOI: 10.31579/2693-7247/031

Garcia A, Doyle TV, Singh R, Price T, Collins K. First report of Curvularia leaf spot of corn, caused by Curvularia lunata, in the United States. Plant Health Progress. 2018;19(2):140-142. https://doi.org/10.1094/PHP-02-18-0008-BR

Bhatt D, Pardeep K. Effect of media, temperature and light wavelength on the growth of Curvularia lunata causing curvularia leaf spot of maize. International Journal of Current Microbiology and Applied Sciences. 2018;7(9): 2227-2230. https:/doi.org/10.20546/ijcmas.2018.709.275

Abrar Ul Hassan MI, Butt I, Haider K, Javaid A, Shad N. Comparative efficacy of three fungicides for in vitro control of Curvularia lunata. Mycopathology. 2021;18(2): 47-52.

Abubakar MN, Likita MS. Effect of combined fungicides on the mycelial growth of Curvularia lunata. International Journal of Innovative Agriculture & Biology Research 2021 9(4):1-9.

Rao SS, Kumar MR, Madhusudhan P, Reddy BR. Comparative efficacy of different plant extracts on mycelial growth of Curvularia lunata (Wakker) Boedijn causing rice grain discoloration in vitro. The Pharma Innovation Journal. 2021;10(5):1151-1155.

Gomes GF, Barbosa GDS, Biancalana A, Biancalana FSC. Evaluation of Antifungal’s Potential Of Plant Extracts Over Pathogenic Fungi.2021 https://periodicos.unifap.br/index.php/biota/article/view/6055

Strobel GA, Hess WM, Ford E, Sidhu, RS, Yang X. 1996. Taxol from fungal endophytes and the issue of biodiversity. Journal of Industrial Microbiology.1996; 17(5):417-423. https://doi.org/10.1007/BF01574772

Iftikhar S, Shahid, AA, Nawaz K, Anwar W. Potential of Trichoderma species as biocontrol agent against Curvularia lunata causing fruit rot of tomato (Lycopersicum esculentum Mill.). 5th International Conference on Food, Agricultural, Biological and Medical Science. 2017; 77-83.

Grover RK, Moore JD. Toximetric Studies Of Fungicides Against Brown Rot Organisms,Sclerotinia-FructicolaAnd S-Laxa. Phytopathology.1962; 52(9):876-880.

Gaba S, Varma A, Prasad R, Goel A. 2022. Exploring the Impact of Bioformulated Copper Oxide Nanoparticles on Cytomorphology of Alternaria brassicicola. Current Microbiology.2022;79(8):1-9. https://doi.org/10.1007/s00284-022-02927-0

Bano A, Mushtaq N, Ahmad M, Mehmood S. Evaluation of antioxidant, antimicrobial, cytotoxic potential and elemental analysis of Filago desertorum whole plant. Indian Journal of Pharmaceutical Sciences. 2021;83(6):1243-1253.DOI: 10.36468/pharmaceutical-sciences.879

Wood PN, Fisher BM. The effect of fungicides on spore germination, mycelial growth and lesion development of Phlyctema vagabunda (syn: Neofabraea alba) (bull’s eye rot of apples). New Zealand Plant Protection. 2017;70(8):112-119. https://doi.org/10.30843/nzpp.2017.70.36

Wang H, Wang J, Chen Q, Wang M, Hsiang T, Shang S, Yu Z. Metabolic effects of azoxystrobin and kresoxim-methyl against Fusarium kyushuense examined using the Biolog FF MicroPlate. Pesticide Biochemistry and Physiology. 2016 Jun 1;130:52-8. https://doi.org/10.1016/j.pestbp.2015.11.013

Kanungo M, Joshi J. Impact of pyraclostrobin (F-500) on crop plants. Plant Science Today. 2014;1(3):174-8. DOI: https://doi.org/10.14719/pst.2014.1.3.60

Hillebrand S, Tietjen K, Zundel JL. Fungicides with unknown mode of action. Modern Crop Protection Compounds. 2019;2:911-32.https://doi.org/10.1002/9783527699261.ch23

Saha S, Chakrabarty PK, Banerjee K. Producing Crops without Mancozeb? Perspectives on Recent Regulatory Dilemmas and Ways Out. ACS Agricultural Science & Technology. 2022;2(2):272-5.https://doi.org/10.1021/acsagscitech.2c00047

Zhao XX, Lin FJ, Li H, Li HB, Wu DT, Geng F, Ma W, Wang Y, Miao BH, Gan RY. Recent advances in bioactive compounds, health functions, and safety concerns of onion (Allium cepa L.). Frontiers in Nutrition. 2021;8. doi: 10.3389/fnut.2021.669805

Xue M, Yi H. Induction of disease resistance providing new insight into sulfur dioxide preservation in Vitis vinifera L. Scientia Horticulturae. 2017;225:567-73.https://doi.org/10.1016/j.scienta.2017.07.055

Adams CA, Zimmerman K, Fenstermacher K, Thompson MG, Skyrud W, Behie S, Pringle A. Fungal seed pathogens of wild chili peppers possess multiple mechanisms to tolerate capsaicinoids. Applied and environmental microbiology. 2020;86(3):e01697-19. DOI: https://doi.org/10.1128/AEM.01697-19

Dong S, Yang X, Zhao L, Zhang F, Hou Z, Xue P. Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Industrial crops and products. 2020;149:112350. https://doi.org/10.1016/j.indcrop.2020.112350

Sharma D, Purohit N, Chaubey S, and Tiwari MA. Review on Pharmacological and Therapeutic Potential of Aloe barbadensis Miller. European Journal of Medicinal Plants. 2022;33(6): 23-43. DOI: 10.9734/EJMP/2022/v33i630471

Desam NR, Al-Rajab AJ, Sharma M, Mylabathula MM, Gowkanapalli RR, Albratty M. Chemical constituents, in vitro antibacterial and antifungal activity of Mentha× Piperita L. (peppermint) essential oils. Journal of King Saud University-Science. 2019 ;31(4):528-33. https://doi.org/10.1016/j.jksus.2017.07.013

Tariq S, Wani S, Rasool W, Shafi K, Bhat MA, Prabhakar A, Shalla AH, Rather MA. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microbial pathogenesis. 2019;134:103580. https://doi.org/10.1016/j.micpath.2019.103580

Ahmad W. Preliminary phytochemical, antimicrobial and photochemical study of Calotropis gigantea leaf extract. Current Chemistry Letters. 2020;9(3):105-12. DOI: 10.5267/j.ccl.2019.10.001

Syawal H, Hakim L, Effendi I. Phytochemical analysis of Rhizophora apiculata leaf extract and its inhibitory action against Staphylococcus aureus, Aeromonas hydrophila and Pseudomonas aeruginosa. Aquaculture, Aquarium, Conservation & Legislation. 2020;13(4):2242-9.

Atiq M, Zafar F, Rajput NA, Sahi S.T, Hameed A, Ali S, Nawaz A. Response of Bell Pepper Germplasm, Phytoextracts And Synthetic Chemicals Towards Southern Blight Caused By Sclerotium Rolfsii Under Field Conditions. Plant Cell Biotechnology and Molecular Biology. 2022;31-42.

Imran A, Kashif UR, Syed IA, Jamil Y, Maria Z. Corporate social responsibility influences, employee commitment and organizational performance. African Journal of Business Management. 2010;4(13):2796-801.

Steel RGD, Torrie JH, Dickey D. Principles and procedure of statistics. A biometrical approach 3rd Ed.1997. McGraw HillBookCo. Inc., New York. 352-358.

Gupta S, Dubey A, Singh T. Curvularia lunata as, a dominant seed-borne pathogen in Dalbergia sissoo Roxb: Its location in seed and its phytopathological effects. African Journal of Plant Science. 2017;11(6):203-8.https://doi.org/10.5897/AJPS2017.1529

Pei Y, Sun Y, Feng T, Chen Y, Long H. Pathogen Identification for the New Leaf Spot Disease of Capsicum annuum L. and Its Biological Characteristics. Chinese Journal of Tropical Crops. 2021;42(9):2659. DOI: 10.3969/j.issn.1000-2561.2021.09.030

Adaangadi KC, Harlapur SI, Savita C, Hadimani BR. Studies on evaluation of fungicides against Curvularia lunata in maize. International Journal of Agricultural Sciences. 2018;14(1):225-8.DOI: 10.15740/HAS/IJAS/14.1/225-228

Bisht S, Balodi R, Ghatak A, Kumar P. Determination of susceptible growth stage and efficacy of fungicidal management of Curvularia leaf spot of maize caused by Curvularia lunata (Wakker) Boedijn. Maydica. 2018;61(3):5.

Singh S, Saxena R, Pandey K, Bhatt K, Sinha S. Response of antioxidants in sunflower (Helianthus annuus L.) grown on different amendments of tannery sludge: its metal accumulation potential. Chemosphere. 2004 ;57(11):1663-73. https://doi.org/10.1016/j.chemosphere.2004.07.049

Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY, Kim JC. Antifungal activity of five plant essential oils as fumigant against postharvest and soilborne plant pathogenic fungi. The Plant Pathology Journal. 2007;23(2):97-102.

Abd-El-Khair H, Haggag WM. Application of some Egyptian medicinal plant extracts against potato late and early blights. Res. J. Agric. Biol. Sci. 2007;3(3):166-75.

Al-Samarrai G, Singh H, Syarhabil M. Evaluating eco-friendly botanicals (natural plant extracts) as alternatives to synthetic fungicides. Annals of Agricultural and Environmental Medicine. 2012;19(4).

Pal GK, Kumar B, Shahi SK. Antifungal activity of some common weed extracts against phytopathogenic fungi Alternaria spp. International journal of universal pharmacy and life sciences. 2013;3(2):6-14.

Danish P, Ali Q, Hafeez MM, Malik A. Antifungal and antibacterial activity of aloe vera plant extract. Biological and Clinical Sciences Research Journal. 2020 ;2020(1). https://doi.org/10.54112/bcsrj.v2020i1.4

Guohui, Z., RongyuL, XiaomaoW, MingL. Inhibitory activity of aloesin and aloe gel against Curvularia lunata. Chinese Journal of Pesticide Science 2021;23:316-322. 10.16801/j.issn.1008-7303.2021.0018

Published

24-06-2023 — Updated on 01-07-2023

Versions

How to Cite

1.
Qaisar MH, Rajput NA, Atiq M, Rehman A, Kachelo GA, Ahmed H, Zafar H, Shaheen HMU, Rani HK. Inhibitory effect of Polyram DF and Capsicum annum on leaf spot of rose caused by Curvularia lunata in vitro and in planta. Plant Sci. Today [Internet]. 2023 Jul. 1 [cited 2024 Apr. 28];10(3):188-96. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2228

Issue

Section

Research Articles

Most read articles by the same author(s)