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Plant Science Today (PST)

Research Articles

Vol. 3 No. 2 (2016)

Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

DOI
https://doi.org/10.14719/pst.2016.3.2.227
Submitted
30 March 2016
Published
06-04-2016

Abstract

The present study established a protocol for suspension cell culture of Stevia rebaudiana and demonstrates the antioxidant enzymes activity during various developing phase of it. Higher concentration of Auxin and Cytokines (3.0 mg L-1 BAP and 4.0 mg L-1 NAA) with Ascorbic acid (1 mg L-1) considered as highly suitable growth regulator combination for growth and development of Stevia rebaudiana suspension culture. Maximum concentration of stevioside (~70 mg G-1 of dry weight of tissue) was obtained on 14th day (exponential phase) which got reduced on 28th day (apoptotic phase) upto ~21 mg G-1 of dry weight of tissue. The amount of MDA reduced initially upto stationary growth phase which showed the adaptation of suspension cells in the culture medium and culture environment. Reduction of Chlorophyll showed the enhancing phenolic content with progressive culture period, while enhanced amount of proline was indicating the generation of defense mechanism with depletion of nutrient (with increased culture duration). The amount of SOD got enhanced with elevating the concentration of H2O2 as well. The concentration of CAT enhanced upto stationary growth phase of suspension cell while APX concentration showed continuous inhibition up to apoptotic phase from exponential phase.

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