Screening of Chickpea genotypes from different agro-climatic areas against Fusarium oxysporum f.sp. ciceris (race 3) using morphological and molecular marker
DOI:
https://doi.org/10.14719/pst.2329Keywords:
Chickpea, Fusarium, Screening, STMS marker, Pot screeningAbstract
Fusarium oxysporum f.sp. ciceris (FOC), an extremely destructive pathogen, infects chickpea plants leading to over 100% losses. Although using chemicals like Carbendazim and Mancozeb control the disease but ruin the soil’s natural flora and fauna. Also, the emergence of new FOC races threatens the current genotypes. Many efforts have been made towards improving chickpea genotypes through breeding and selection, but the situation has not been improved over the last 2 decades. The current research uses pot screening and molecular-based approaches to screen out the resistant chickpea cultivars. In that view, the present research uses 16 chickpea genotypes collected from diverse agro-climatic areas and checked against FOC race-3. After the pot screening and ANOVA (P<0.001), the genotypes were categorized as highly Resistant (C 235, HC 1), resistant (GNG 2477, PHULeG 0517, GNG 2171, HC 7, PHULe G 0127), susceptible (ICCV 10) and highly susceptible (PUSA 547, RSG 931, RSG 888, ICCV 512, CSJ 513, ICCV 6). In Marker-assisted selection (MAS), the DNA of genotypes was subjected to PCR with STMS markers TA-96 and TA-27. The results revealed that the genotypes ICCV 512, C 235, GNG 2171, ICCV 10, HC 7, PHULe G 0127 and HC 1 were resistant. These results are significant for selecting resistant genotypes and can be utilized in the future validation and development of more wilt-resistant chickpea genotypes. Our results based on pot-screening and molecular-based datasets suggested a more reliable identification system for screening of FOC resistance cultivar inhibiting, which can help narrow down the selection.
Downloads
References
Khanzada N, Iqbal O, Baby A, Zaman S, Tofique M, Rajput A. Evaluation of chickpea germplasm for relative resistance or susceptibility against Fusarium wilt and Ascochyta blight under field conditions. Plant Protection. 2022;6(2):121-32. https://doi.org/10.33804/pp.006.02.4234
Chickpea [Internet]. Faostat. [cited 2023, Feb17]. Available from: https://www.fao.org/faostat/en/#data/QCL
Bharadwaj C, Jorben J, Rao A, Roorkiwal M, Patil BS, Jayalakshmi et al. Development of high yielding Fusarium wilt resistant cultivar by pyramiding of “genes” through marker-assisted backcrossing in Chickpea (Cicer arietinum L.). Frontiers in Genetics. 2022;13. https://doi.org/10.3389/fgene.2022.924287
Jha UC, Bohra A, Pandey S, Parida SK. Breeding, genetics and genomics approaches for improving Fusarium wilt resistance in major grain legumes. Frontiers in Genetics. 2020;11. https://doi.org/10.3389/fgene.2020.01001
Dhawale SN, Dhale DA. Effects of Fusarium wilt on chickpea in India: A review. 6. International Journal of Botany Studies; 2021.
Mannur DM, Babbar A, Thudi M, Sabbavarapu MM, Roorkiwal M, Yeri SB et al. Super annigeri 1 and improved JG 74: Two Fusarium wilt-resistant introgression lines developed using marker-assisted backcrossing approach in chickpea (Cicer arietinum L.). Molecular Breeding. 2018;39(1): https://doi.org/10.1007/s11032-018-0908-9
Sahu VK, Tiwari S, Tripathi MK, Gupta N, Tomar RS, Ahuja A et al. Molecular marker validation and identification of Fusarium wilt resistant chickpea genotypes. Indian Journal of Genetics and Plant Breeding. 2020;80(2): https://doi.org/10.31742/IJGPB.80.2.6
Venkataramanamma K, Reddy BVB, Jayalakshmi RS, Jayalakshmi V, Prasad KVH. Identification of races of Fusarium oxysporum f.sp. ciceris, inciting wilt of chickpea in Andhra Pradesh and parts of Telangana. Legume Research - An International Journal. 2020;(Of). https://doi.org/10.18805/LR-4393
Gupta Neha, Bhagyawant Sameersuresh. Testing DNA variation in Kabuli chickpea seeds through ISSR-PCR. Innovare Journal of Life Sciences. 2021;1-4. https://doi.org/10.22159/ijls.2021.v9i4.42229
Suthar KP, Rajkumar BK, Parmar PR, Singh D. Molecular mechanism underlying chickpea – Fusarium oxysporum f. sp. ciceris interaction. Sustainable Agriculture Reviews. 2021;:213-45. https://doi.org/10.1007/978-3-030-68828-8_8
Singh PK, Prakash C, Pandey S, Singh KK, Parihar SS, Singh AK. STMS markers proved effective in establishing identity and determining genetic purity in commercial seed lots of ‘Pusa Rice Hybrid 10’. International Journal of Agriculture, Environment and Biotechnology. 2018;11(1):79-85.
Sharma KD, Muehlbauer FJ. Fusarium wilt of chickpea: Physiological specialization, genetics of resistance and resistance gene tagging. Euphytica. 2007;157(1-2):1-14. https://doi.org/10.1007/s10681-007-9401-y
Lal D, Ravikumar RL, Jingade P, Subramanya S. Validation of molecular markers linked to Fusarium wilt resistance in recombinant inbred lines of chickpea (Cicer arietinum L.). 2021; https://doi.org/10.21203/rs.3.rs-366182/v1
Kukreja S, Salaria N, Thakur K, Goutam U. Fungal disease management in Chickpea: Current status and future prospects. Fungi and their role in sustainable development: current perspectives. 2018;293-309. https://doi.org/10.1007/978-981-13-0393-7_17
Khan BA, Freed S, Zafar J, Farooq M, Shoukat RF, Ahmad KW, Li S, Zhang Y, Hua Y, Shoukat RF. Efficacy of different entomopathogenic fungi on biological parameters of pulse beetle Callosobruchus chinensis L. (Coleoptera: Bruchidae). J Entomol Zool Stud. 2018;6:1972-76.
Patidar JK, Singh PK, Singh R, Pandya RK. Assessment of artificial inoculation techniques for development of chickpea dry root rot caused by Rhizoctonia bataticola. The Pharma Innovation Journal. 2022;
Rani R, Tripathi S, Srinivasa N, Kumari N, Singh G. Genetic resistance in chickpea (Cicer arietinum L.) against race 3 and 4 of Fusarium wilt. Indian Phytopathology. 2022 Sep;75(3):713-21. https://doi.org/10.1007/s42360-022-00512-w
Yadav SH, Kumar S. Screening and evaluation of Cicer arietinum genotypes against Fusarium wilt under sick field and artificial condition. 21. Asian Journal of Microbiolgy, Biotechnology and Environment Science. 2019;
Sager SR, Kumar SA. Detection and evaluation of Cicer arietinum genotypes against collar rot under sick field and artificial condition. 21. Plant Cell Biotechnology and Molecular Biology. 2021;
Anwar R, Ali S, Zeshan MA, Ghani MU, Ali A, Iftikhar Y, Bakhsh A. Effect of Fusarium wilt disease on varied Chickpea germplasm and its management. Journal of Pure and Applied Agriculture. 2022;7(1):1-9.
Srivastava AK, Dixit GP, Singh NP, Saxena DR, Saabale PR, Raghuvanshi KS, Anandani VP. Evaluation of chickpea (Cicer arietinum L.) accessions for Fusarium wilt resistance. Journal of Environmental Biology. 2021;42(1):82-89. https://doi.org/10.22438/jeb/42/1/MRN-1475
Tatte R, Mane SS, Jukte S, Jadhav P, Nandanwar RS, Patil AN. Marker assisted selection for mapping population against chickpea wilt. International Journal of Current Microbiology and Applied Sciences. 2018;7(6):2480-86. https://doi.org/10.20546/ijcmas.2018.706.294
Amadabade J, Arora A, Sahu H, Singh MK. Molecular characterization of Fusarium wilt resistance and confirmation of hybridity in chickpea genotypes. 22. Ecology, Environment and Conservation. 2016;
Singh AP, Singh RK, Hegde VS, Shukla N, Saini P, Yadav R, Yadav N, Aggarwal R, Kumar R. Unclasping potential chickpea resources for the antioxidant enzyme superoxide dismutase. JSFA Reports. 2022 Jul;2(7):320-31. https://doi.org/10.1002/jsf2.65
Gowda CL, Singh O, Sethi SC, Singh KB, Rao BV, Rahman MM et al. Registration of ‘ICCV 10’ chickpea. Crop Science. 1995;35(2):589. https://doi.org/10.2135/cropsci1995.0011183X003500020054x
Global information system. Available from: https://ssl.fao.org/glis/doi/10.18730/MAR97
Downloads
Published
Versions
- 19-07-2023 (3)
- 01-07-2023 (2)
- 23-06-2023 (1)
How to Cite
Issue
Section
License
Copyright (c) 2022 Samiksha, Sarvjeet Kukreja, Vinod Goyal, Sanjeev Kumar
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
