In vitro antifungal activities of Cymbopogon citratus, Cymbopogon martini, Pogestemon cablin and Curcuma longa essential oils against spoilage fungi isolated from tomatoes

Rajib Sarkar; Farjana Showline  Chaity; Md Saddam Hossain; Farhana Yeasmin; Jannatul Ferdous; GM Masud  Rana; Md Saidur  Rahman; Nemai Chandra  Nandi; Habibur Rahman  Bhuiyan; Mohammad Mostafa; Saiful Islam

doi:10.14719/pst.2815

Authors

Rajib Sarkar Industrial Microbiology Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0000-0003-2616-0419
Farjana Showline Chaity Industrial Microbiology Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0000-0002-4420-6057
Md Saddam Hossain Industrial Microbiology Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0000-0001-9101-0260
Farhana Yeasmin Department of Biochemistry and Molecular Biology, Noakhali Science and Technology University, Noakhali 3814, Bangladesh https://orcid.org/0000-0002-4204-818X
Jannatul Ferdous Industrial Microbiology Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0009-0003-2217-428X
GM Masud Rana BCSIR Rajshahi Laboratories, Bangladesh Council of Scientific and Industrial Research, Rajshahi 6206, Bangladesh https://orcid.org/0009-0007-1977-3096
Md Saidur Rahman Industrial Botany Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research, Chattogram-4220, Bangladesh https://orcid.org/0000-0002-4455-9602
Nemai Chandra Nandi Industrial Botany Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research, Chattogram-4220, Bangladesh https://orcid.org/0009-0001-7994-2521
Habibur Rahman Bhuiyan Institute of Glass and Ceramic Research and Testing, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh https://orcid.org/0000-0001-6336-7326
Mohammad Mostafa Phytochemistry Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0000-0001-8886-1937
Saiful Islam Industrial Microbiology Research Division, BCSIR Chattogram Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram-4220, Bangladesh https://orcid.org/0000-0003-2228-9186

DOI:

https://doi.org/10.14719/pst.2815

Keywords:

Antifungal activity, essential oils, Solanum lycopersicum, spoilage fungi

Abstract

Tomatoes (Solanum lycopersicum L.) have highmoisture content (93–95%), making them more vulnerable to various types of spoilage fungi. Numerous medicinal plants' essential oils are known to have antifungal properties that combat these spoilage fungi. Therefore, this study aimed to investigate the potential antifungal activity of essential oils from some indigenous plants in Bangladesh to prevent the fungal attack on tomatoes. Using potato dextrose agar medium, 4 species of spoilage fungi from tomatoes were identified. ITS (Internal transcribed spacer) region-specific sequencing was used to identify the fungal species as Aspergillus welwitschiae, Aspergillus tamari, Penicillium citrinum and Rhizopus arrhizus. Assessment on antifungal activity was conducted for four essential oils extracted from plants: turmeric leaf (Curcuma longa), patchouli (Pogestemon cablin), palmarosa (Cymbopogon martini) and lemongrass (Cymbopogon citratus). The GC-MS method was used to analyze these essential oils to ascertain their chemical content. A pathogenicity test determined the severity and % of rot on tomatoes. A. welwitschiae caused the most severe spoilage (80%) after 21 days, while R. arrhizus damaged 26% of the tomatoes within 5 days. The minimal fungicidal concentration (MFC) and minimal inhibitory concentration (MIC) of the essential oils were determined after analysis. Lemongrass oil fully suppressed the growth of all the isolates at a concentration of 12 ????l/ml. Finally, the essential oils of palmarosa and lemongrass may be used as a preventative measure against tomato fungal rot.

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