This is an outdated version published on 03-09-2024. Read the most recent version.

Forthcoming

Inducible overexpression of MYB118-like gene improves anthocyanin production in Populus × canescens

Authors

Wei Zhou (1) Key Laboratory of Three Gorges Regional Plant Genetic & Germplasm Enhancement (CTGU)/ Biotechnology Research Center, China Three Gorges University, Yichang, 443 002, Hubei, China (2) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (3) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China
Zhengquan He Key Laboratory of Three Gorges Regional Plant Genetic & Germplasm Enhancement (CTGU)/ Biotechnology Research Center, China Three Gorges University, Yichang, 443 002, Hubei, China
Yuhong Li (1) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (2) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0009-0009-8374-2467
Yuneng Yao (1) Key Laboratory of Three Gorges Regional Plant Genetic & Germplasm Enhancement (CTGU)/ Biotechnology Research Center, China Three Gorges University, Yichang, 443 002, Hubei, China (2) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (3) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0009-0003-1777-0095
Xiaojiao Han (1) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (2) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0000-0001-6082-7569
Guirong Qiao (1) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (2) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0000-0003-3313-2424
Jing Xu (1) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (2) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0000-0003-0265-1969
Renying Zhuo (1) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (2) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0000-0002-7063-3714
Wenmin Qiu (1) Key Laboratory of Three Gorges Regional Plant Genetic & Germplasm Enhancement (CTGU)/ Biotechnology Research Center, China Three Gorges University, Yichang, 443 002, Hubei, China (2) State Key Laboratory of Tree Genetic and Breeding, Chinese Academy of Forestry, Beijing, China (3) Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311 400, China https://orcid/org/0000-0002-4593-2165

DOI:

https://doi.org/10.14719/pst.2879

Keywords:

Anthocyanin biosynthesis, MYB118L, Populus canescens, genetic engineering, Rd29A promoter

Abstract

Anthocyanins are secondary metabolites which contribute different colors to the leaves. Anthocyanin biosynthesis is regulated by MYB transcription factors, which have been extensively studied and characterized in a diversity of plants. In this study, we identified a novel MYB transcription factor MYB118L, from Populus 'Zhonghua Hongye', which showed an elevated accumulation of anthocyanin than Populus clone ZL-2025. Subcellular localization analyses revealed that MYB118L-GFP fusion protein was specifically located in the nucleus. Transgenic plants overexpressing MYB118L driven by the stress-inducible Rd29A promoter showed a significant increase in anthocyanin production, resulting in a red coloration of the leaves under drought stress conditions. These plants also exhibited higher expression levels of genes involved in anthocyanin biosynthesis compared to the wild type, suggesting that MYB118L positively regulates the expression of these genes. Y1H and dual-luciferase assays confirmed that MYB118L can directly activate the promoters of LAR1 gene. Our findings suggest that MYB118L is an essential transcription factor involved in the regulation of anthocyanin biosynthesis in poplar and could be utilized for genetic engineering of colorful tree species.

Downloads

Download data is not yet available.

References

Zhao MH, Li X, Zhang XX et al. Mutation mechanism of leaf color in plants: A review. Forests. 2020;11(8):851. https://doi.org/10.3390/f11080851

Hong L, Zhuo L. The classification of colorful-leaf tree species and the application in landscape architecture. Journal of Northeast Agricultural University (English Edition). 2008;01(30):70-72.

Yi Y. Application of colored-leaf plants in landscape construction. Journal of Landscape Research. 2015;7(6):89.

Harmut A. Chlorophylls and carotenoids. Pigments of photosynthetic membranes. Methods Enzymol. 1987;148:350-83. https://doi.org/10.1016/0076-6879(87)48036-1

Markham KR. Flavones, flavonols and their Glycosides. Methods in Plant Biochemistry. 1989;1:197-235. https://doi.org/10.1016/B978-0-12-461011-8.50012-3

Chalker-Scott L. Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology. 1999;70(1):1-9. https://doi.org/10.1111/j.1751-1097.1999.tb01944.x

Jaakola, Laura. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends in Plant Science. 2013;18(9):477-83. https://doi.org/10.1016/j.tplants.2013.06.003

Jaakola L, Poole M, Jones MO et al. A SQUAMOSA MADS box gene involved in the regulation of anthocyanin accumulation in bilberry fruits. Plant Physiology. 2010;(4):1619-29. https://doi.org/10.1104/pp.110.158279

Xu W, Grain D, Bbet S et al. Complexity and robustness of the flavonoid transcriptional regulatory network revealed by comprehensive analyses of MYB-bHLH-WDR complexes and their targets in Arabidopsis seed. New Phytologist. 2014;202(1):15-18. https://doi.org/10.1111/nph.12620

Xu W, Dubos C, Lepiniec LC. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes. Trends in Plant Science. 2015;20(3):176-85. https://doi.org/10.1016/j.tplants.2014.12.001

Mathews H, Clendennen SK, Caldwell CG et al. Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification and transport. Plant Cell. 2003;15(8):1689-703. https://doi.org/10.1105/tpc.012963

Nakatsuka T, Haruta KS, Pitaksutheepong C et al. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant and Cell Physiology. 2008;49(12):1818-29. https://doi.org/10.1093/pcp/pcn163

Mano H, Ogasawara F, Sato K et al. Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato. Plant Physiology. 2007;143(3):1252-68. https://doi.org/10.1104/pp.106.094425

Dubos C, Stracke R, Grotewold E et al. MYB transcription factors in Arabidopsis. Trends in Plant Science. 2010;15(10):573-81. https://doi.org/10.1016/j.tplants.2010.06.005

Zimmermann IM, Heim MA, Weisshaar B et al. Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins. Plant Journal. 2004;40(1):22-34. https://doi.org/10.1111/j.1365-313X.2004.02183.x

Yang C, Li D, Liu X et al. OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.). BMC Plant Biology. 14:1-15. https://doi.org/10.1186/1471-2229-14-158

Arun, Kumaran, Anguraj et al. GmMYB176 regulates multiple steps in isoflavonoid biosynthesis in soybean. Frontiers in Plant Science. 2019;10:562-62. https://doi.org/10.3389/fpls.2019.00562

Qiu W, Liu M, Qiao G et al. An isopentyl transferase gene driven by the stress-inducible rd29A promoter improves salinity stress tolerance in transgenic tobacco. Plant Molecular Biology Reporter. 2012;30:519-28. https://doi.org/10.1007/s11105-011-0337-y

Paz-Ares J, Ghosal D, Wienand U et al. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. Embo Journal. 1987;6(12):3553-58. https://doi.org/10.1002/j.1460-2075.1987.tb02684.x

Borevitz, JO. Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell. 2000;12(12):2383-94. https://doi.org/10.1105/tpc.12.12.2383

Espley RV, Hellens RP, Putterill J et al. Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant Journal. 2010;49(3):414-27. https://doi.org/10.1111/j.1365-313X.2006.02964.x

Gonzalez A, Zhao MZ, Leavitt JM et al. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant Journal. 2008;53(5):814-27. https://doi.org/10.1111/j.1365-313X.2007.03373.x

Sun CL, Deng L, Du MM et al. A transcriptional network promotes anthocyanin biosynthesis in tomato flesh. Molecular Plant. 2020;13:42-58. https://doi.org/10.1016/j.molp.2019.10.010

Quattrocchio F, Wing J, van der Woude K et al. Molecular analysis of the anthocyanin1 gene of petunia and its role in the evolution of flower color. Plant Cell. 1999;11:1433-44. https://doi.org/10.1105/tpc.11.8.143

Ban Y, Honda C, Hatsuyama Y et al. Isolation and functional analysis of a MYb transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant and Cell Physiology. 2007;48:958-70. https://doi.org/10.1093/pcp/pcm066

Khan IA, Cao K, Guo J et al. Identification of key gene networks controlling anthocyanin biosynthesis in peach flower. Plant Science. 2022;316:111151. https://doi.org/10.1016/j.plantsci.2021.111151

Li YQ, Shan XT, Tong LN et al. The conserved and particular roles of the R2R3-MYb regulator FhPAP1 from Freesia hybrida in flower anthocyanin biosynthesis. Plant and Cell Physiology. 2020;61:1365-80. https://doi.org/10.1093/pcp/pcaa065

Li CH, Qiu J, Yang GS et al. Isolation and characterization of a R2R3-MYb transcription factor gene related to anthocyanin biosynthesis in the spathes of Anthurium andraeanum (Hort.). Plant Cell Reporter. 2016;35:2151-65. https://doi.org/10.1007/s00299-016-2025-8

Liu YF, Ma KX, Qi YW et al. Transcriptional regulation of anthocyanin synthesis by MYB-bHLH-WDR complexes in kiwi fruit (Actinidia chinensis). Journal of Agricultural and Food Chemistry. 2021;69:3677-91. https://doi.org/10.1021/acs.jafc.0c07037

Li Z, Ahammed GJ. Plant stress response and adaptation via anthocyanin. Plant Stress. 2023;100230. https://doi.org/10.1016/j.stress.2023.10023

Downloads

Published

03-09-2024

Versions

01-10-2024 (2)
03-09-2024 (1)

How to Cite

Zhou W, He Z, Li Y, Yao Y, Han X, Qiao G, Xu J, Zhuo R, Qiu W. Inducible overexpression of MYB118-like gene improves anthocyanin production in Populus × canescens. Plant Sci. Today [Internet]. 2024 Sep. 3 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2879

Download Citation

Issue

Early Access

Section

Research Articles

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright and Licence details of published articles

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Open Access Policy

Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).