Advanced Mutant Line Developed from Fatemadhan Shows Salinity Tolerance at both Seedling and Reproductive Stages

Authors

DOI:

https://doi.org/10.14719/pst.2917

Keywords:

Rice mutants, phenotypic variability, salinity tolerance, correlation co-efficient, principal component analysis, stress tolerance indices

Abstract

The generation of high-yielding rice mutants and their assessment under salt stress offers a great possibility to isolate salt tolerant line(s) with desired trait of interest. Two separate experiments were conducted at the seedling and reproductive stages of rice to assess the level of salinity tolerance of few advanced high-yielding rice mutants. In the first experiment, rice seedlings were grown under hydroponic conditions and 14-day-old seedlings were subjected to salt stress (EC=10 dS/m; 7 days). Salt stress caused significant reduction in root and shoot length and biomass and leaf chlorophyll content; however, a little reduction was found in the mutant Line-1. In contrast, a sharp increase in shoot Na+/K+ ratio was found in all the genotypes except, Binadhan-10, FL-478 and the mutant Line-1, which exhibited little increased ratio. The second experiment involved exposure of plant to salt stress (EC=10 dS/m) for three weeks at the late booting stage in a sizable plastic tub filled with field soil. Salt stress resulted in a significant decrease in yield and yield attributing traits in all the genotypes except Binadhan-10. Grain yield per panicle was found significantly positive correlation with panicle length, the number of filled grains per panicle, and 100-seed weight under both control and salt stress conditions. Based on the studied traits and stress tolerance indices, Binadhan-10 and mutant Line-1 categorized as salt tolerant and rest of the genotypes were categorized as susceptible, which is also evident from the biplot of principal component analysis. Considering the results from both of the experiments, mutant Line-1 was found tolerant genotype at both seedling and reproductive stage. However, further studies are required to determine the genetic issues controlling the salinity tolerance in mutant Line-1 and the high-yield potential of mutant Line-65 under control condition in a way to develop salt tolerant and high-yielding rice varieties, respectively.

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References

Jaiswal S, Gautam R, Singh R, Krishnamurthy S, Ali S, Sakthivel K, Iquebal M, Rai A, Kumar D. Harmonizing technological advances in phenomics and genomics for enhanced salt tolerance in rice from a practical perspective. Rice. 2019;12:1-19. https://doi.org/10.1186/s12284-019-0347-1.

Ganie SA, Molla KA, Henry RJ, Bhat KV, Mondal TK. Advance in understanding salt tolerance in rice. Theoretical and Applied Genetics. 2019;132(4):851-70. https://doi: 10.1007/s00122-019-03301-8.

Food and Agriculture Organization (FAO). Production/Yield Quantities of Rice, Paddy in World. Available online: http://www.fao.org/faostat/en/#data/QC/visualize (accessed on 3 August 2020). Food and Agriculture Organization of the United Nations. 2020.

Salehin M, Chowdhury MMA, Clarke D, Mondal S, Nowreen S, Jahiruddin M, Haque A. Mechanisms and drivers of soil salinity in Coastal Bangladesh. In: Nicholls R, Hutton CW, Adger W, Hanson S, Rahman M, Salehin M, editors. Ecosystem services for well-being in deltas. Cham: Palgrave Macmillan. 2018;p. 333-47. https://doi.org/10.1007/978-3-319-71093-8_18

Dasgupta S, Hossain MM, Huq M, Wheeler D. Climate change, salinization and high-yield rice production in coastal Bangladesh. Agricultural and Resource Economics Review. 2018;47(1):66-89. https://doi.org/10.1017/age.2017.14.

Rao PS, Mishra B, Gupta SR, Rathore A. Reproductive stage tolerance to salinity and alkalinity stresses in rice genotypes. Plant Breeding. 2008;127(3):256-61.https://doi.org/10.1111/j.1439-0523.2007.01455.x

Mostofa MG, Hossain MA, Fujita M. Trehalose pretreatment induces salt tolerance in rice seedlings: Oxidative damage and co-induction of antioxidant defense and glyoxalase systems. Protoplsasma. 2015;252(2):461-75. https://doi.org/10.1007/s00709-014-0691-3.

Kamran M, Parveen A, Ahmar S, Malik Z, Hussain S, Chattha MS. An overview of hazardous impacts of soil salinity in crops, tolerance mechanisms and amelioration through selenium supplementation. International Journal of Molecular Sciences. 2020;21:148. https://doi.org/10.3390/ijms21010148.

Acosta-Motos JR, Ortuño MF, Bernal-Vicente A, Diaz-Vivancos P, Sanchez-Blanco MJ, Antonio-Hernandez J. Plant responses to salt stress: adaptive mechanisms. Agronomy. 2017;7:18. https://doi.org/10.3390/agronomy7010018

Assaha DVM, Ueda A, Saneoka H, Yahyai RA, Yaish MW. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers in Physiology. 2017;8:509. https://doi.org/10.3389/fphys.2017.00509.

Ashraf M, Foolad MR. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany. 2007;59:206-16. http://dx.doi.org/10.1016/j.envexpbot.2005.12.006.

Singh RK, Kota S, Flowers TJ. Salt tolerance in rice: Seedling and reproductive stage QTL mapping come of age. Theoretical and Applied Genetics. 2021;134:3495-33. https://doi.org/10.1007/s00122-021-03890-3.

Joshi R, Prasat R, Sharma PC, Singla-pareek SL, Pareek A. Physiological characterization of gamma ray induced mutant population of rice to facilitate biomass and yield improvement under salinity stress. Indian Journal of Plant Physiology. 2016;21(4):545-55. https://doi.org/10.1007/s40502-016-0264-x.

Chowdhury N, Islam S, Mim MH, Akter S, Naim J, Nowicka B, Hossain MA. Characterization and genetic analysis of the selected rice mutant populations. SABRAO Journal of Breeding and Genetics. 2023;55(1):25-37. http://doi.org/10.54910/sabrao2023.55.1.

Thomas RL, Sheard RW, Moyer JR. Comparison of conventional and automated procedures for nitrogen, phosphorous and potassium analysis of plant material using a single digestion. Agronomy Journal. 1967;59(3):240-43. https://doi.org/10.2134/agronj1967.00021962005900030010x.

Fernandez GC. Effective selection criteria for assessing plant stress tolerance. In: Kuo CG, editor. Proceedings of the international symposium on adaptation of vegetables and other food crops in temperature and water stress. Tainan: AVRDC Publication. 1992; p. 257-70.

Rosielle AA, Hamblin J. Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science. 1981;21:943-46. http://dx.doi.org/10.2135/cropsci1981.0011183X002100060033x.

Fischer KS, Maurer R. Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research. 1978;29:897-912. http://dx.doi.org/10.1071/AR9780897.

Bouslama M, Schapaugh WT. Stress tolerance in soybeans: Evaluation of three screening techniques for heat and drought tolerance. Crop Science. 1984;24(5):933-37. https://doi.org/10.2135/cropsci1984.0011183X002400050026x.

Haque MA, Rafii MY, Yusoff MM, Ali NS, Yusuff O, Datta DR, Anisuzzaman M, Ikbal MF. Advanced breeding strategies and future perspectives of salinity tolerance in rice. Agronomy. 2021;11(8):1631. https://doi.org/10.3390/agronomy11081631

Kakar N, Jumaa SH, Redoña ED, Warburton ML, Reddy KR. Evaluating rice for salinity using pot-culture provides a systematic tolerance assessment at the seedling stage. Rice. 2018;12:57. https://doi.org/10.1186/s12284-019-0317-7.

Rasel M, Tahjib-Ul-Arif M, Hossain MA, Sayed MA, Hassan L. Discerning of rice landraces (Oryza sativa L.) for morpho-physiological, antioxidant enzyme activity and molecular markers responses to induced salt stress at the seedling stage. Journal of Plant Growth Regulation. 2021;39:41-59. https://doi.org/10.1007/s11738-018-2645-4.

Rahnama A, James AR, Poustini K, Munns R. Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil. Functional Plant Biology. 2010;37(3):255-63. https://doi.org/10.1071/FP09148.

Munns R, Tester M. Mechanisms of salinity tolerance. Annual Review of Plant Biology. 2008;59:651-81. https://doi.org/10.1146/annurev.arplant.59.032607.092911.

Castillo EG, Tuong TP, Ismail AM, Inubushi K. Response to salinity in rice: Comparative effects of osmotic and ionic stresses. Plant Production Science. 2007;10:159-70. https://doi.org/10.1626/pps.10.159.

Zhang Y, Fang J, Wu X, Dong L. Na+/K+ balance and transport regulatory mechanisms in weedy and cultivated rice (Oryza sativa L.) under salt stress. BMC Plant Biology. 2018;18:1-14. https://doi.org/10.1186/s12870-018-1586-9.

Omisun T, Sahoo S, Saha B, Panda SK. Relative salinity tolerance of rice cultivars native to North East India: A physiological, biochemical and molecular perspective. Protoplasma. 2018;255:193-202. https://doi.org/10.1007/s00709-017-1142-8.

Mahmood U, Hussain S, Hussain S, Ali B, Ashraf U, Zamir S. Morpho-physio-biochemical and molecular responses of maize hybrids to salinity and waterlogging during stress and recovery phase. Plants. 2021;10:1345. https://doi.org/10.3390/plants10071345.

Nahar L, Aycan M, Hanamata S, Baslam M, Mitsui T. Impact of single and combined salinity and high-temperature stresses on agrophysiological, biochemical and transcriptional responses in rice and stress release. Plants. 2022;11:501. https://doi.org/10.3390/plants11040501.

Rasel M, Hassan L, Hoque MIU, Saha SR. Estimation of genetic variability, correlation and path coefficient analysis in local landraces of rice (Oryza sativa L.) for the improvement of salinity tolerance. Journal of Bangladesh Agricultural University. 2018;16(1):41-46. doi: 10.3329/jbau.v16i1.36479

Faseela P, Sinisha AK, Brestic M, Puthur JT. Chlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice. Photosynthetica. 2019;57:108-15. https://doi.org/10.32615/ps.2019.147.

Moradi F, Ismail AM. Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Annals of Botany. 2007;99(6):1161-73. https://doi.org/10.1093/aob/mcm052.

Rahman MM, Jahan I, Al Noor MM, Tuzzohora MF, Sohag AAM, Raffi SA, Islam MM, Burritt DJ, Hossain MA. Potential determinants of salinity tolerance in rice (Oryza sativa L.) and modulation of tolerance by exogenous ascorbic acid application. Journal of Phytology. 2020;12:86-98. https://doi.org/10.25081/jp.2020.v12.6535.

Munns R, James RA, Läuchli A. Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany. 2006;57:1025-43. https://doi.org/10.1093/jxb/erj100.

Flexas J, Diaz-Espejo AN, Galmes J, Kaldenhoff R, Medrano H, Ribas-Carbo MI. Rapid variations of mesophyll conductance in response to changes in CO2 concentration around leaves. Plant, Cell and Environment. 2007;30:1284-98. https://doi.org/10.1111/j.1365-3040.2007.01700.x.

Munns R, James RA, Gilliham M, Flowers TJ, Colmer TD. Tissue tolerance: An essential but elusive trait for salt-tolerant crops. Functional Plant Biology. 2016;43:1103-13. https://doi.org/10.1071/FP16187

Roy SJ, Negrão S, Tester M. Salt resistant crop plants. Current Opinion in Biotechnology. 2014;26:115-24. https://doi.org/10.1016/j.copbio.2013.12.004.

Mitsuya S, Yano K, Kawasaki M, Taniguchi M, Miyak H. Relationship between the distribution of Na+ and the damages caused by salinity in the leaves of rice seedlings grown under a saline condition. Plant Production Science. 2002;5(4):269-74. https://doi.org/10.1626/pps.5.269.

Ismail AM, Horie T. Genomics, physiology and molecular breeding approaches for improving salt tolerance. Annual Review of Plant Biology. 2017;68(1):405-34. https://doi.org/10.1146/annurev-arplant-042916-040936.

Rao PS, Mishra B, Gupta SR, Rathore A. Reproductive stage tolerance to salinity and alkalinity stresses in rice genotypes. Plant Breeding. 2008;127(3):256-61. https://doi.org/10.1111/j.1439-0523.2007.01455.x.

Chattopadhyay K, Nayak AK, Marndi BC, Poonam A, Chakraborty K, Sarkar RK. Novel screening protocol for precise phenotyping of salt-tolerance at reproductive stage in rice. Physiology and Molecular Biology of Plants. 2018;24(6):1047-58. https://doi.org/10.1007/s12298-018-0591-7.

Gerona MEB, Deocampo MP, Egdane JA, Ismail AM, Dionisio-Sese ML. Physiological responses of contrasting rice genotypes to salt stress at reproductive stage. Rice Science. 2019;26(4):207-19. https://doi.org/10.1016/j.rsci.2019.05.001

Krishnamurthy SL, Gautam RK, Sharma PC, Sharma DK. Effect of different salt stresses on agro-morphological traits and utilisation of salt stress indices for reproductive stage salt tolerance in rice. Field Crop Research. 2016;190:26-33. https://doi.org/10.1016/j.fcr.2016.02.018.

Abdullah Z, Khan MA, Flowers TJ. Causes of sterility in seed set of rice under salinity stress. Journal of Agronomy and Crop Science. 2001;187(1):25-32. https://doi.org/10.1046/j.1439-037X.2001.00500.x.

Al-Ashkar I, Alderfasi A, El-Hendawy S, Al-Suhaibani N, El-Kafafi S, Seleiman MF. Detecting salt tolerance in doubled haploid wheat lines. Agronomy. 2019;9:211. https://doi.org/10.3390/agronomy9040211.

Jahan I, Rahman MM, Tuzzohora1 MF, Hossain MA, Begum SN, Burritt DJ, Hossain MA. Phenotyping of mungbean (Vigna radiata L.) genotypes against salt stress and assessment of variability for yield and yield attributing traits. Journal of Plant Stress Physiology. 2020;6:07-17. https://doi.org/10.25081/jpsp.2020.v6.6111.

Robin AHK, Matthew C, Uddin MJ, Bayazid KN. Salinity-induced reduction in root surface area and changes in major root and shoot traits at the phytomer level in wheat. Journal of Experimental Botany. 2016;67(12):3719-29. https://doi.org/10.1093/jxb/erw064.

Wijewardana C, Henry WB, Hock MW, Reddy KR. Growth and physiological trait variation among corn hybrids for cold tolerance. Canadian Journal of Plant Science. 2016;96:639-56.http://doi.org/10.1139/CJPS-2015-0286.

Tabassum R, Tahjib-Ul-Arif M, Hasanuzzaman M, Sohag AAM, Islam MS, Shafi SMSH, Islam MM, Hassan L. Screening salt-tolerant rice at the seedling and reproductive stages: An effective and reliable approach. Environmental and Experimental Botany. 2021;192:104629. https://doi.org/10.1016/j.envexpbot.2021.104629.

Krishnamurthy SL, Sharma SK, Gautam RK, Kumar V. Path and association analysis and stress indices for salinity tolerance traits in promising rice (Oryza sativa L.) genotypes. Cereal Research Communication. 2014;42(3):474-83. https://doi.org/10.1556/CRC.2013.0067.

Published

14-12-2023 — Updated on 05-01-2024

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Khatun T, Khatun SM, Mim MH, Naim J, Jahin SA, Hossain MR, El-Esawi MA, Hossain MA. Advanced Mutant Line Developed from Fatemadhan Shows Salinity Tolerance at both Seedling and Reproductive Stages. Plant Sci. Today [Internet]. 2024 Jan. 5 [cited 2024 Nov. 21];11(1):316-28. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2917

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