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Influence of some chemicals and solvents on the lytic activity and the adsorption of bacteriophages on Pectobacterium carotovoroum Subsp. carotovorum

Authors

Mohammed H Abdel-Aal Plant Research Department, Nuclear Research Center (NRC), Egyptian Atomic Energy Authority (EAEA), Cairo 13759, Egypt https://orcid.org/0000-0002-2078-8662
Yasmeen A Hasanien Plant Research Department, Nuclear Research Center (NRC), Egyptian Atomic Energy Authority (EAEA), Cairo 13759, Egypt https://orcid.org/0000-0002-4368-4903
Nahed A Younis Plant Research Department, Nuclear Research Center (NRC), Egyptian Atomic Energy Authority (EAEA), Cairo 13759, Egypt https://orcid.org/0009-0002-2692-1556
Gamal El Didamony Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt https://orcid.org/0000-0001-8804-5753
Ahmed Askora Department of Microbiology and Botany, Faculty of Science, Zagazig University, Zagazig 44519, Egypt https://orcid.org/0000-0002-8465-0208
Naglaa M Balabel Bacterial Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619,Egypt https://orcid.org/0000-0001-9199-1361
Khaled Abdelaal Agricultural Botany Department, EPCRS Excellence Center, Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture, Kafr Elsheikh University, 33516, Egypt. https://orcid.org/0000-0001-7295-6560

DOI:

https://doi.org/10.14719/pst.2998

Keywords:

Pectobacterium carotovorum subsp. carotovorum, Soft rot, Bacteriophages, Pesticides, Fertilizers, Solvents

Abstract

Recently, bacteriophages have been used to control hazardous bacterial soft rot disease on crops. However, agricultural plants are frequently treated with different chemicals (fertilizers, pesticides and solvents), so we assessed the effect of some commonly used chemicals and solvents on the lytic activity of tested bacteriophages and their adsorption potential. This study reports the isolation of three specific phages against the Pectobacterium carotovorum subsp. carotovorum DSM 30170 strain, designated as ?PC1, ?PC2 and ?PC3, then partially characterized using electron microscopy and genome size. The 3 isolated phages belong to the Myoviridae family. The results obtained were based on the plaque-forming unit observed after incubation. By increasing the chemical concentrations (from 0.1 to 0.5 mM), calcium chloride (CaCl2) and potassium chloride (KCl) showed a significant increase in the lytic activity of the phages. Copper sulphate (CuSO4) and copper chloride (CuCl2) showed a substantial decrease in the activity of ?PC3; however, such a decrease was insignificant for ?PC1 and ?PC2. By increasing the solvent concentrations (from 30 % v/v to 70 % v/v), propanol, ethanol and methanol showed a significant decrease in the count of the three isolated phages, ?PC1, ?PC2 and ?PC3, compared to the control. Chloroform was the only solvent that did not reduce the phage titer. Our findings offer significant information for developing a strategy to combat the P. carotovorum subsp. carotovorum caused bacterial soft rot disease. avoiding copper compounds and alcoholic solvents such as propanol, ethanol and methanol in plots where phages are applied seems advisable.

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How to Cite

Abdel-Aal MH, Hasanien YA, Younis NA, Didamony GE, Askora A, Balabel NM, Abdelaal K. Influence of some chemicals and solvents on the lytic activity and the adsorption of bacteriophages on Pectobacterium carotovoroum Subsp. carotovorum. Plant Sci. Today [Internet]. 2024 Jan. 16 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2998

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