Green Synthesis of Cobalt Oxide Nanoparticles with In-vitro Cytotoxicity Assessment Using Pomegranate (Punica granatum L.) Seed Oil: A Promising Approach for Antimicrobial and Anticancer Applications
DOI:
https://doi.org/10.14719/pst.3014Keywords:
green synthesis, Punica granatum seed oil, cobalt oxide nanoparticles, antimicrobial, anticancer cytotoxicityAbstract
Green synthesis of nanoparticles and their pharmacological implementation have gained importance in the field of nanotechnology. This study primarily aims to explore the use of Punica granatum L. seed oil as a reducing agent for the synthesis of cobalt nanoparticles, making it both economically and pharmacologically valuable. Gas chromatography-mass spectroscopy analysis was carried out to study the active metabolites present in P. granatum seed oil. The green synthesis of cobalt nanoparticles was established based on the color change of the reaction mixture from dark green to light green. These particles showed a ?max at 279.88 nm for UV-visible spectrometry analysis. Furthermore, X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscope (FE SEM) and Dynamic Light Scattering (DLS) were performed to confirm the nature of these nanoparticles. The pharmacological potential of these cobalt oxide nanoparticles was tested against microbial pathogens. The results suggest that these nanoparticles exhibited significant activity against various human bacterial and fungal pathogens. Additionally, in in vitro cytotoxicity analysis, demonstrated that CoONPs selectively targeted MCF-7 cancer cells with a significant IC50 value compared to non-cancerous cells (L929). In conclusion, this study demonstrated that green synthesized CoONPs using P. granatum show significant potential against eukaryotic cancer cells and microbial pathogens. Furthermore, this study has implications for medical research centers and pharmaceutical industries in addressing modern challenges such as increasing antibiotic resistance in communities.
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References
Benelmekki M. An introduction to nanoparticles and nanotechnology. In designing hybrid nanoparticles. Morgan & Claypool Publishers; Apr 1 2015.
Vo-Dinh T. Nanotechnology in biology and medicine: Methods, devices and applications. CRC Press; 2017. https://doi.org/10.4324/9781315374581
Zhang D, Ma XL, Gu Y, Huang H, Zhang GW. Green synthesis of metallic nanoparticles and their potential applications to treat cancer. Frontiers in Chemistry. 2020;8:799. https://doi.org/10.3389/fchem.2020.00799
Taya SA, Colak I, Suthar B, Ramahi OM. Cancer cell detector based on a slab waveguide of anisotropic, lossy and dispersive left-handed material. Applied Optics. 2021;60(27):8360-67. https://doi.org/10.1364/AO.437738
Abdelghany TM, Al-Rajhi AM, Al Abboud MA, Alawlaqi MM, Ganash Magdah A, Helmy EA, Mabrouk AS. Recent advances in green synthesis of silver nanoparticles and their applications: About future directions. A review. BioNanoScience. 2018;8:5-16. https://doi.org/10.1007/s12668-017-0413-3
Rafique M, Sadaf I, Rafique MS, Tahir MB. A review on green synthesis of silver nanoparticles and their applications. Artificial Cells, Nanomedicine and Biotechnology. 2017;45(7):1272-91. https://doi.org/10.1080/21691401.2016.1241792
Ravi L, Sreenivas BA, Kumari GS, Archana O. Anticancer cytotoxicity and antifungal abilities of green-synthesized cobalt hydroxide (Co(OH) 2) nanoparticles using Lantana camara L.. Beni-Suef University Journal of Basic and Applied Sciences. 2022;11(1):124. https://doi.org/10.1186/s43088-022-00304-1
Hafeez M, Shaheen R, Akram B, Haq S, Mahsud S, Ali S, Khan RT. Green synthesis of cobalt oxide nanoparticles for potential biological applications. Materials Research Express. 2020;7(2):025019. https://doi.org/10.1088/2053-1591/ab70dd
Bibi I, Nazar N, Iqbal M, Kamal S, Nawaz H, Nouren S et al. Green and eco-friendly synthesis of cobalt-oxide nanoparticle: Characterization and photo-catalytic activity. Advanced Powder Technology. 2017;28(9):2035-43. https://doi.org/10.1016/j.apt.2017.05.008
Shahbazi Y. Chemical composition and in vitro antibacterial effect of Ziziphora clinopodioides essential oil. Pharmaceutical Sciences. 2015;21(2):51-56. https://doi.org/10.15171/PS.2015.17
Malathy D, Revathi M. Green synthesis of cobalt nanoparticles using ethanolic extract of Cadiospermum halicacebium characterisation and its anticancer applications. Research Journal of Pharmacy and Technology. 2021;14(3):1530-34. https://doi.org/10.5958/0974-360X.2021.00271.7
Singh J, Dutta T, Kim KH, Rawat M, Samddar P, Kumar P. Green synthesis of metals and their oxide nanoparticles: Applications for environmental remediation. Journal of Nanobiotechnology. 2018;16(1):1-24. https://doi.org/10.1186/s12951-018-0408-4
Kahramanoglu I, Usanmaz S. Pomegranate production and marketing. CRC Press; 2016. https://doi.org/10.1201/b20151
Yang X, Niu Z, Wang X, Lu X, Sun J, Carpena M et al. The nutritional and bioactive components, potential health function and comprehensive utilization of pomegranate: A review. Food Reviews International. 2023;39(9):6420-46. https://doi.org/10.1080/87559129.2022.2110260
Bhandari PR. Pomegranate (Punica granatum L). ancient seeds for modern cure? Review of potential therapeutic applications. International Journal of Nutrition, Pharmacology, Neurological Diseases. 2012;2(3):171-84. https://doi.org/10.4103/2231-0738.99469
Melgarejo-Sánchez P, Nunez-Gomez D, Martínez-Nicolás JJ, Hernández F, Legua P, Melgarejo P. Pomegranate variety and pomegranate plant part, relevance from bioactive point of view: A review. Bioresources and Bioprocessing. 2021;8:1-29. https://doi.org/10.1186/s40643-020-00351-5
Boroushaki MT, Mollazadeh H, Afshari AR. Pomegranate seed oil: A comprehensive review on its therapeutic effects. Int J Pharm Sci Res. 2016;7(2):430.
Hontecillas R, O’Shea M, Einerhand A, Diguardo M, Bassaganya-Riera J. Activation of PPAR gamma and alpha by punicic acid ameliorates glucose tolerance and suppresses obesity-related inflammation. Journal of the American College of Nutrition. 2009;28(2):184-95. https://doi.org/10.1080/07315724.2009.10719770
Fu Y, Li J, Almasi M. Pomegranate peel extract-mediated green synthesis of silver nanoparticles: Evaluation of cytotoxicity, antioxidant and anti-esophageal cancer effects. ChemistrySelect. 2023;8(15):e202204841. https://doi.org/10.1002/slct.202204841
Khan AA, Alanazi AM, Alsaif N, Wani TA, Bhat MA. Pomegranate peel induced biogenic synthesis of silver nanoparticles and their multifaceted potential against intracellular pathogen and cancer. Saudi Journal of Biological Sciences. 2021;28(8):4191-200. https://doi.org/10.1016/j.sjbs.2021.06.022
Prasad C, Karlapudi S, Venkateswarlu P, Bahadur I, Kumar S. Green arbitrated synthesis of Fe3O4 magnetic nanoparticles with nanorod structure from pomegranate leaves and Congo red dye degradation studies for water treatment. Journal of Molecular Liquids. 2017;240:322-28. https://doi.org/10.1016/j.molliq.2017.05.100
Mohamed F, Rabia M, Shaban M. Synthesis and characterization of biogenic iron oxides of different nanomorphologies from pomegranate peels for efficient solar hydrogen production. Journal of Materials Research and Technology. 2020;9(3):4255-71. https://doi.org/10.1016/j.jmrt.2020.02.052
Thanh NT, Green LA. Functionalisation of nanoparticles for biomedical applications. Nano today. 2010;5(3):213-30. https://doi.org/10.1016/j.nantod.2010.05.003
Ifeanyichukwu UL, Fayemi OE, Ateba CN. Green synthesis of zinc oxide nanoparticles from pomegranate (Punica granatum) extracts and characterization of their antibacterial activity. Molecules. 2020;25(19):4521. https://doi.org/10.3390/molecules25194521
Franzolin MR, Courrol DD, Silva FR, Courrol LC. Antimicrobial activity of silver and gold nanoparticles prepared by photoreduction process with leaves and fruit extracts of Plinia cauliflora and Punica granatum. Molecules. 2022;27(20):6860. https://doi.org/10.3390/molecules27206860
Ivanova E, Atanasova-Pan?evska N, Kungulovski D. Antimicrobial activities of laboratory produced essential oil solutions against five selected fungal strains. Zbornik Matice Srpske Za Prirodne Nauke. 2013;124:171-83. https://doi.org/10.2298/ZMSPN1324171I
Kadhim DA, Abid MA, Abdulghany ZS, Yahya Alhatem J, Kadhim SA, Aziz WJ, Al-Marjani MF. Iron oxide nanoparticles synthesized using plant (Beta vulgaris and Punica granatum) extracts for a breast cancer cell line (MCF-7) cytotoxic assay. Materials Technology. 2022;37(13):2436-44. https://doi.org/10.1080/10667857.2022.2038766
Govindappa M, Tejashree S, Thanuja V, Hemashekhar B, Srinivas C, Nasif O et al. Pomegranate fruit fleshy pericarp mediated silver nanoparticles possessing antimicrobial, antibiofilm formation, antioxidant, biocompatibility and anticancer activity. Journal of Drug Delivery Science and Technology. 2021;61:102289. https://doi.org/10.1016/j.jddst.2020.102289
Akhlaghi N, Najafpour-Darzi G, Younesi H. Facile and green synthesis of cobalt oxide nanoparticles using ethanolic extract of Trigonella foenumgraceum (Fenugreek) leaves. Advanced Powder Technology. 2020;31(8):3562-69. https://doi.org/10.1016/j.apt.2020.07.004
Kang W, Yang H, Hong HJ, Han CH, Lee YJ. Anti-oxidant activities of kiwi fruit extract on carbon tetrachloride-induced liver injury in mice. Korean Journal of Veterinary Research. 2012;52(4):275-80. https://doi.org/10.14405/kjvr.2012.52.4.275
Hou H, Mahdavi B, Paydarfard S, Zangeneh MM, Zangeneh A, Sadeghian N et al. Retracted article: Novel green synthesis and antioxidant, cytotoxicity, antimicrobial, antidiabetic, anticholinergics and wound healing properties of cobalt nanoparticles containing Ziziphora clinopodioides Lam. leaves extract. Scientific Reports. 2020;10(1):12195. https://doi.org/10.1038/s41598-020-68951-x
Chau TP, Veeraragavan GR, Narayanan M, Chinnathambi A, Alharbi SA, Subramani B et al. Green synthesis of zirconium nanoparticles using Punica granatum (pomegranate) peel extract and their antimicrobial and antioxidant potency. Environmental Research. 2022;209:112771. https://doi.org/10.1016/j.envres.2022.112771
Nisha MH, Tamileaswari R, Jesurani S. Analysis of anti-bacterial activity of silver nanoparticles from pomegranate (Punica granatum) seed and peel extracts. Int J Eng Res Technol. 2015;4(4):1044-48. https://doi.org/10.17577/IJERTV4IS041104
Sukri SN, Shameli K, Wong MM, Teow SY, Chew J, Ismail NA. Cytotoxicity and antibacterial activities of plant-mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract. Journal of Molecular Structure. 2019;1189:57-65. https://doi.org/10.1016/j.molstruc.2019.04.026
Hashem AH, Saied E, Ali OM, Selim S, Al Jaouni SK, Elkady FM, El-Sayyad GS. Pomegranate peel extract stabilized selenium nanoparticles synthesis: Promising antimicrobial potential, antioxidant activity, biocompatibility and hemocompatibility. Applied Biochemistry and Biotechnology. 2023;195:1-24. https://doi.org/10.1007/s12010-023-04326-y
Sadrolhosseini AR, Noor AS, Husin MS, Sairi NA. Green synthesis of gold nanoparticles in pomegranate seed oil stabilized using laser ablation. Journal of Inorganic and Organometallic Polymers and Materials. 2014;24:1009-13. https://doi.org/10.1007/s10904-014-0090-4
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