Methyl jasmonate mitigates biochemical and phytochemical changes in salt stressed Stevia rebaudiana plants
DOI:
https://doi.org/10.14719/pst.3033Keywords:
Methyl jasmonate, salt stress, Stevia rebaudiana Bertoni, oxidative stress, prolineAbstract
This study explored the effects of salt stress on growth, oxidative stress, and steviol glycoside content in Stevia rebaudiana Bertoni plants cultivated in soil supplemented with methyl jasmonate (MeJA). Stevia plants were cultivated under normal and salt stress conditions, with and without MeJA supplements of 30 ?M, 60 ?M, and 120 ?M. Samples were harvested after the 1st, 3rd, 5th, 10th, and 15th day of treatment. The levels of chlorophyll (p<0.0001), carotenoids (p<0.0001), and antioxidant enzymes such as catalase (p<0.0001), superoxide dismutase (p<0.0001), APX (p<0.0001), and glutathione reductase (p<0.0001) were observed. The quantification of steviol glycosides, including stevioside (p<0.0001) and rebaudioside-A (p<0.0001), was studied by the most advanced hyphenated technique, LC-MS/MS. The study revealed that the oxidative stress responses were significantly improved in MeJA-treated plants compared to salt-stress control plants. The level of production of phenols (p<0.0001), flavonoids, total sugar, reducing sugar, and steviol glucosides was significantly altered in salt-stress plants. MeJA showed a dose- and time-dependent significant effect on the improvement of these factors over salt stress. In conclusion, MeJA not only improves the growth of plants but also reduces oxidative stress and enhances the level of phytochemicals under saline stress.
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Jan SA, Habib N, Shinwari ZK, Ali M, Ali N. The anti-diabetic activities of natural sweetener plant Stevia: an updated review. SN Applied Sciences. 2021;3:1-6. https://doi.org/10.1007/s42452-021-04519-2
Shukla S, Mehta A. Comparative phytochemical analysis and in vivo immunomodulatory activity of various extracts of Stevia rebaudiana leaves in experimental animal model. Frontiers in Life Science. 2015; 8(1):55-63. https://doi.org/10.1080/21553769.2014.961615
Sharma R, Yadav R, Manivannan E. Study of effect of Stevia rebaudiana Bertoni on oxidative stress in type-2 diabetic rat models. Biomedicine & Aging Pathology. 2012;2(3):126-31. https://doi.org/10.1016/j.biomag.2012.07.001
Rajesh P, Kannan VR, Durai MT. Effect of Stevia rebaudiana Bertoni ethanolic extract on anti-cancer activity of Erlisch’s Ascites carcinoma induced mice. Journal of Current biotica. 2010;3(4).
Tadhani MB, Subhash R. In vitro antimicrobial activity of Stevia rebaudiana Bertoni leaves. Tropical Journal of Pharmaceutical Research. 2006;5(1):557-60. https://doi.org/10.4314/tjpr.v5i1.14633
Singh DP, Rajiv, Kumari M, Prakash HG, Kumar P. Augmenting commercial yield of Stevia (Stevia rebaudiana) through agronomic interventions in Indian sub-tropics. Sugar Tech. 2022. https://doi.org/10.1007/s12355-022-01110-w
Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10(3). https://doi.org/10.38212/2224-6614.2748
Gardana C, Simonetti P, Canzi E, Zanchi R, Pietta P. Metabolism of stevioside and rebaudioside A from Stevia rebaudiana extracts by human microflora. Journal of Agricultural and Food Chemistry. 2003;51(22):6618-22. https://doi.org/10.1021/jf0303619
Karako?se H, Mu?ller A, Kuhnert N. Profiling and quantification of phenolics in Stevia rebaudiana leaves. Journal of Agricultural and Food Chemistry. 2015;63(41):9188-98. https://doi.org/10.1021/acs.jafc.5b01944
Lemus-Mondaca R, Vega-Galvez A, Zura-Bravo L, Ah-Hen K. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem. 2012;132(3):1121-32. https://doi.org/10.1016/j.foodchem.2011.11.140
Nabeta K, Kasai T, Sugisawa H. Phytosterol from the callus of Stevia rebaudiana Bertoni. Agric Biol Chem. 1976;40(10):2103-04. https://doi.org/10.1080/00021369.1976.10862360
Kumar P, Sharma PK. Soil salinity and food security in India. Frontiers in Sustainable Food Systems. 2020;4:533781. https://doi.org/10.3389/fsufs.2020.533781
Song F-n, Yang C-p, Liu X-m, Li G-b. Effect of salt stress on activity of superoxide dismutase (SOD) in Ulmus pumila L. Journal of Forestry Research. 2006;17(1):13-16. https://doi.org/10.1007/s11676-006-0003-7
Rasool A, Shah WH, Mushtaq NU, Saleem S, Hakeem KR, ul Rehman R. Amelioration of salinity induced damage in plants by selenium application: A review. S Afr J Bot. 2022;147:98-105. https://doi.org/10.1016/j.sajb.2021.12.029
Moradi P, Vafaee Y, Mozafari AA, Tahir NA-r. Silicon nanoparticles and methyl jasmonate improve physiological response and increase expression of stress-related genes in strawberry cv. Paros under salinity stress. Silicon. 2022;14(16):10559-69. https://doi.org/10.1007/s12633-022-01791-8
Zandi P, Schnug E. Reactive oxygen species, antioxidant responses and implications from a microbial modulation perspective. Biology. 2022;11(2):155. https://doi.org/10.3390/biology11020155
Yoon JY, Hamayun M, Lee S-K, Lee I-J. Methyl jasmonate alleviated salinity stress in soybean. Journal of Crop Science and Biotechnology. 2009;12:63-68. https://doi.org/10.1007/s12892-009-0060-5
Dhankot Z, Sanghvi G. Salicylic acid improving physiological and phytochemical changes in Stevia rebaudiana under salt stress and estimating steviol glycoside by LC-MS/MS. Research Journal of Biotechnology. 2023;19(1):110-21. https://doi.org/10.25303/1901rjbt1100121
Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144(3):307-13. https://doi.org/10.1016/S0176-1617(11)81192-2
Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39(1):205-07. https://doi.org/10.1007/bf00018060
Ková?ik J, Klejdus B, Hedbavny J, Ba?kor M. Salicylic acid alleviates NaCl-induced changes in the metabolism of Matricaria chamomilla plants. Ecotoxicology. 2009;18(5):544-54. https://doi.org/10.1007/s10646-009-0312-7
Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276-87. https://doi.org/10.1016/0003-2697(71)90370-8
Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22(5):867-80. https://doi.org/10.1093/oxfordjournals.pcp.a076232
Zhang Z, Pang X, Xuewu D, Ji Z, Jiang Y. Role of peroxidase in anthocyanin degradation in litchi fruit pericarp. Food Chem. 2005;90(1-2):47-52. https://doi.org/10.1016/j.foodchem.2004.03.023
Aebi H. Catalase in vitro. Methods Enzymol. Elsevier. 1984;105:p. 121-26. https://doi.org/10.1016/S0076-6879(84)05016-3
Antuono LF, Moretti A, Lovato AF. Seed yield, yield components, oil content and essential oil content and composition of Nigella sativa L. and Nigella damascena L. Industrial Crops and Products. 2002;15(1):59-69. https://doi.org/10.1016/S0926-6690(01)00096-6
Weatherley P. Studies in the water relations of the cotton plant. I. The field measurement of water deficits in leaves. New Phytol. 1950;81-97. https://doi.org/10.1111/j.1469-8137.1950.tb05146.x
Miller GL, Blum R, Glennon WE, Burton AL. Measurement of carboxymethylcellulase activity. Anal Biochem. 1960;1(2):127-32. https://doi.org/10.1016/0003-2697(60)90004-X
Yemm E, Willis A. The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal. 1954;57(3):508. https://doi.org/10.1042/bj0570508
Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry. 2003;51(3):609-14. https://doi.org/10.1021/jf020782a
Wagner GJ. Content and vacuole/extravacuole distribution of neutral sugars, free amino acids and anthocyanin in protoplasts. Plant Physiol. 1979;64(1):88-93. https://doi.org/10.1104/pp.64.1.88
Hasanuzzaman M, Alam M, Rahman A, Hasanuzzaman M, Nahar K, Fujita M. Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. BioMed Research International. 2014;2014. https://doi.org/10.1155/2014/757219
Hinai MSA, Ullah A, Al-Rajhi RS, Farooq M. Proline accumulation, ion homeostasis and antioxidant defence system alleviate salt stress and protect carbon assimilation in bread wheat genotypes of Omani origin. Environ Exp Bot. 2022;193:104687. https://doi.org/10.1016/j.envexpbot.2021.104687
Porgali ZB, Yurekli F. Salt stress-induced alterations in proline accumulation, relative water content and superoxide dismutase (SOD) activity in salt sensitive Lycopersicon esculentum and salt-tolerant L. pennellii. Acta Botanica Hungarica. 2005;47(1-2):173-82. https://doi.org/10.1556/abot.47.2005.1-2.15
Taïbi K, Taïbi F, Ait Abderrahim L, Ennajah A, Belkhodja M, Mulet JM. Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L. S Afr J Bot. 2016;105:306-12. https://doi.org/10.1016/j.sajb.2016.03.011
Sultana N, Ikeda T, Itoh R. Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environ Exp Bot. 1999;42(3):211-20. https://doi.org/10.1016/S0098-8472(99)00035-0
Salimi F, Shekari F, Hamzei J. Methyl jasmonate improves salinity resistance in German chamomile (Matricaria chamomilla L.) by increasing activity of antioxidant enzymes. Acta Physiologiae Plantarum. 2015;38(1):1. https://doi.org/10.1007/s11738-015-2023-4
Anjum S, Wang L, Farooq M, Khan I, Xue L. Methyl jasmonate?induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought. Journal of Agronomy and Crop Science. 2011;197(4):296-301. https://doi.org/10.1111/j.1439-037X.2011.00468.x
Fedina I, Tsonev T. Effect of pretreatment with methyl jasmonate on the response of Pisum sativum to salt stress. J Plant Physiol. 1997;151(6):735-40. https://doi.org/10.1016/S0176-1617(97)80071-5
Kumar V, Khare T, Sharma M, Wani SH. ROS-induced signaling and gene expression in crops under salinity stress. In: Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress. 2017:159-84. https://doi.org/10.1007/978-981-10-5254-5_7
Kukreja S, Nandwal A, Kumar N, Sharma S, Sharma S, Unvi V, et al. Plant water status, H2O2 scavenging enzymes, ethylene evolution and membrane integrity of Cicer arietinum roots as affected by salinity. Biol Plant. 2005;49:305-08. https://doi.org/10.1007/s10535-005-5308-4
Pan Y, Wu LJ, Yu ZL. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regulation. 2006;49:157-65. https://doi.org/10.1007/s10725-006-9101-y
Faghih S, Ghobadi C, Zarei A. Response of strawberry plant cv. ‘camarosa’ to salicylic acid and methyl jasmonate application under salt stress condition. J Plant Growth Regul. 2017;36(3):651-59. https://doi.org/10.1007/s00344-017-9666-x
Sadeghipour O. Amelioration of salinity tolerance in cowpea plants by seed treatment with methyl jasmonate. Legume Research- An International Journal. 2017;40(6):1100-06. https://doi.org/10.18805/lr.v0i0.8394
Gulmezoglu N, Aydogan C, Turhan E. Physiological, biochemical and mineral dimensions of green bean genotypes depending on Zn priming and salinity. Legume Research- An International Journal. 2016;39(5):713-21. https://doi.org/10.18805/lr.v0iOF.3543
Li H, Zhu Y, Hu Y, Han W, Gong H. Beneficial effects of silicon in alleviating salinity stress of tomato seedlings grown under sand culture. Acta Physiologiae Plantarum. 2015;37:1-9. https://doi.org/10.1007/s11738-015-1818-7
Cha-um S, Charoenpanich A, Roytrakul S, Kirdmanee C. Sugar accumulation, photosynthesis and growth of two indica rice varieties in response to salt stress. Acta Physiologiae Plantarum. 2009;31(3):477-86. https://doi.org/10.1007/s11738-008-0256-1
Lucho SR, do Amaral MN, Auler PA, Bianchi VJ, Ferrer MÁ, Calderón AA, et al. Salt stress-induced changes in in vitro cultured Stevia rebaudiana Bertoni: Effect on metabolite contents, antioxidant capacity and expression of steviol glycosides-related biosynthetic genes. J Plant Growth Regul. 2019;38:1341-53. https://doi.org/10.1007/s00344-019-09937-6
Sakamoto M, Suzuki T. Methyl jasmonate and salinity increase anthocyanin accumulation in radish sprouts. Horticulturae. 2019;5(3):62. https://doi.org/10.3390/horticulturae5030062
Zeng J, Chen A, Li D, Yi B, Wu W. Effects of salt stress on the growth, physiological responses and glycoside contents of Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry. 2013;61(24):5720-26. https://doi.org/10.1021/jf401237x
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