Genetic fingerprinting distinguishes pathogenic variability of asiatic citrus canker pathogen, Xanthomonas citri pv. citri type A

Rummana  Islam; Ismam Ahmed  Protic; Abu Sina Md  Tushar; Mehedi  Hasan; Sadia Haque  Tonny; Md Hasibul  Hasan; Irfan  Khan; Uday Rana  Singha; Ayesha  Sultana; Sabera  Akter; Mst. Arjina  Akter; Md. Rashidul Islam

doi:10.14719/pst.3119

Authors

Rummana Islam (1) Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh (2) Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur-1700, Bangladesh https://orcid.org/0009-0002-2616-5981
Ismam Ahmed Protic Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0001-9890-8911
Abu Sina Md Tushar Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0009-0007-8772-3189
Mehedi Hasan Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0009-0001-3394-3596
Sadia Haque Tonny Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0003-3869-1516
Md Hasibul Hasan Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0009-0008-0150-5680
Irfan Khan Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0009-0000-0014-1194
Uday Rana Singha Department of Agricultural Extension, Ministry of Agriculture, Dhaka-1215 https://orcid.org/0009-0004-9233-6827
Ayesha Sultana Department of Agricultural Extension, Ministry of Agriculture, Dhaka-1215 https://orcid.org/0009-0008-5921-291X
Sabera Akter Department of Agricultural Extension, Ministry of Agriculture, Dhaka-1215 https://orcid.org/0009-0004-2713-1577
Mst. Arjina Akter Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0003-0409-7586
Md. Rashidul Islam Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh https://orcid.org/0000-0002-0790-6521

DOI:

https://doi.org/10.14719/pst.3119

Keywords:

Citrus canker, Xanthomonas citri, Pathotype A, Pathogenicity, Aggressiveness

Abstract

Citrus canker, caused by Xanthomonas citri pv. citri (Xcc), is one of the most widespread citrus disease. In this study, 54 isolates of Xcc were obtained from citrus canker-infected leaves, twigs and fruit samples collected from various citrus-growing regions in Bangladesh. These isolates were analysed through pathogenicity tests and PCR amplification. The results revealed varying levels of aggressiveness among the isolates, ranging from weak to moderate and aggressive. No correlation was found between the host plants and their geographical locations. The genetic analysis of Xanthomonas isolates from around the world revealed 100 % similarity. All the Xanthomonas isolates were grouped into 2 major clusters. DNA fingerprinting showed high genetic variability, with no correlation between hosts, geographic locations or aggressiveness levels. This information on the population diversity of the citrus canker pathogen, Xanthomonas citri pv. citri (Xcc), will be valuable for developing sustainable management strategies for citrus cankers in Bangladesh and the broader Indian sub-continent region.

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