Traditional use of Lablab purpureus (L.) Sweet as an abortifacient and antifertility among Karbi tribe of Assam, North East India

Reena Terangpi; Rosni  Jabin; Dwimu  Basumatary; Farishta  Yasmin

doi:10.14719/pst.3380

Authors

Reena Terangpi Department of Botany, Gauhati University, Guwahati-781014, India https://orcid.org/0000-0002-3873-9068
Rosni Jabin Department of Botany, Nowgong College (Autonomous), Nagaon - 782001, India https://orcid.org/0000-0003-1000-8033
Dwimu Basumatary Department of Botany, Nowgong College (Autonomous), Nagaon - 782001, India https://orcid.org/0009-0006-5122-6861
Farishta Yasmin Department of Botany, Nowgong College (Autonomous), Nagaon - 782001, India https://orcid.org/0000-0002-2828-9307

DOI:

https://doi.org/10.14719/pst.3380

Keywords:

Abortifacient, antifertility, ADME, arsenous acid, bioactive compound, Lablab purpureus, Medicinal plants

Abstract

Lablab purpureus (L.) Sweet, a multipurpose leguminous plant is traditionally used as an antifertility and abortifacient agent among the Karbi, an indigenous tribe living in Assam, North East India. The present study emphasizes the scientific validation of the indigenous utilization of this plant extract as an antifertility and abortifacient along with their physicochemical, phytochemical, and in silico ADME features. Fresh roots (450 gm) are processed for physicochemical, preliminary phytochemical screening, and GC-MS analysis. Organoleptic studies, moisture content, swelling index, flow properties (Carr’s index and Hausner ratio), extractive values (highest in aqueous extract), and ash content revealed the plants are potential sources of phytomedicines having the minimum required parameters as per WHO standards. The value of Carr’s index (22%) and Hausner's ratio (1.27) indicates that the powder drugs have greater inter-particle interactions, which signifies poor flowing powder. Phytochemical screening of root extracts showed the presence of flavonoids, tannins, phenolic compounds, alkaloids, coumarins, and terpenoids. GC-MS analysis revealed the presence of Arsenous acid tris(trimethylsilyl; Cyclic siloxane compounds; Pivalic Acid, 2-Methylpropyl Ester; Propanoic Acid, 2,2-Dimethyl-, Ethyl Ester and N-(Trifluoroacetyl)-N, O, O', O''-Tetrakis (Trimethylsilyl) Norepinephrine, having potential reproductive and developmental toxicity and also stimulates progesterone production. According to SwissADME, drug-like properties of Arsenous Acid, Tri (Trimethylsilyl) Ester, Heptasiloxane, and Pivalic Acid, etc., identified in the root extract have the potential for use as an orally active antifertility and abortifacient medicine. Since L. purpureus (L.) Sweet purpureus been used traditionally by the Karbi tribe for ages and contains bioactive compounds showing antifertility and abortifacient activities, it may be considered an important ethnomedicine which may aids in further drugs discovery process.

Downloads

Download data is not yet available.

References

World Health Organization. Quality control methods for herbal materials. World Health Organization, 2011. https:/apps.who.int/iris/handle/10665/444479. Accessed on 25 December 2023.

Katiyar C, Gupta A, Kanjilal S, Katiyar S. Drugs discovery from plant sources: An integrated approach. Ayu. 2013; 33 (1). https://doi.org/10.4103/0974-8520.100295.

Sofowara A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013; 10 (15): 210-29.

Teronpi V, Singh HT, Tamuli A K, Teron R. Ethnozoology of the Karbis of Assam, India: Use of ichthyofauna in traditional health-care practices. Anc Sci life. 2012; 32 (2), 99–103. https://doi.org/10.4103/0257-7941.118547

Terangpi R, Yasmin F. Medicinal Plants used as Abortifacient among Karbis of Assam, India. J. Nat. Remedies. 2021; 21(4), 297–302. https://doi.org/10.18311/jnr/2021/26142

Singh A, Abhilash PC.Varietal dataset of nutritionally important Lablab purpureus (L.) Sweet from Eastern Uttar Pradesh, India. Data in brief, 2019; 24 103935, 1-10.

https://www.sciencedirect.com/science/article/pii/S2352340919302860

Valenzuela H, Smith J. Green Manure crops: Lablab, College of Tropical Agriculture and Human Resources. 2002; 1-10.

Kanjilal UN, Kanjilal PC, Das A. Flora of Assam, Assam Govt. Press, Shillong. 1997; Reprint, Vol. 1-5.

WFO: Lablab purpureus (L.) Sweet. https:/www.worldfloraonline.org/taxon/wfo-0000181603. 2024. Accessed 20 January 2024.

Lablab purpureus (L.) Sweet. India Biodiversity Portal; Available from

https://indiabiodiversity.org/observation/show/17856568. Accessed on 02 Feb 2024.

Lablab purpureus (L.) Sweet. International Plant Names Index; Available from https://www.ipni.org/n/90501-3. Accessed on 2 Feb 2024.

Khandelwal, K.R. Practical Pharmacognosy.19th Ed. Nirali Prakashan Publications. 2008.

Das BK, Al-Amin MM, Russel SM, Kabir S, Bhattacherjee R, Hannan JM (2014). Phytochemical Screening and Evaluation of Analgesic Activity of Oroxylum indicum. Indian J. Pharm. Sci., 2014; 76(6), 571–575.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293694/

Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. Sci World J. 2017; 5873648. https://doi.org/10.1155/2017/5873648.

Singh PK, Singh J, Medhi T, Kumar A. Phytochemical Screening, Quantification, FT-IR Analysis, and in Silico Characterization of Potential Bio-active Compounds Identified in HR-LC/MS Analysis of the Polyherbal Formulation from Northeast India. ACS Omega. 2022; 7 (37), 33067-78. https://doi.org/10.1021/acsomega.2c03117.

SwissADME. Swiss Institute of Bioinformatics; available from http://www.swissadme.ch/ accessed on 02-02-2024.

Asati BS, Yadav DS (2004). Diversity of horticultural crops in North Eastern Region. ENVIS Bulletin: Himalayan Ecology. 2004; 12(1): 1–10.

https://kiran.nic.in/pdf/publications/Diversity_of_Horticulture.pdf

Sumbul S, Ahmad MA, Asif M, Akhtar M, Saud I. Physicochemical and phytochemical standardization of berries of Myrtus communis Linn. J Phar Bioallied Sci. 2012; 4(4), 322–26. https://doi.org/10.4103/0975-7406.103266.

Etti CJ, Yusof YA, Chin NL, Tahir SM. Flowability properties of Labisia pumila herbal powder. Agriculture and Agricultural Science Procedia. 2014; 2:120-27.

Winterbottom EF, Ban Y, Sun X, Capobianco AJ, Marsit CJ, Chen X, Wang L, Karagas MR, Robbins DJ. Transcriptome-wide analysis of changes in the fetal placenta associated with prenatal arsenic exposure in the New Hampshire Birth Cohort Study. Environ Health. 2019; 18:100. https://doi.org/10.1186/s12940-019-0535-x.

Quansah R, Armah FA, Essumang DK, Luginaah I, Clarke E, Marfoh K, Cobbina SJ, Nketiah-Amponsah E, Namujju PB, Obiri S, Dzodzomenyo M. (2015). Association of arsenic with adverse pregnancy outcomes/infant mortality: a systematic review and meta-analysis. Environmental health perspectives. 2015; 123(5), 412–21.

https://doi.org/10.1289/ehp.1307894.

Ortiz-Garcia NY, Cipriano Ramírez AI, Juarez K, Brand Galindo J, Briceño G, Calderon Martinez E. Maternal Exposure to Arsenic and Its Impact on Maternal and Fetal Health: A Review. Cureus. 2023; 15(11), e49177. https://doi.org/10.7759/cureus.49177.

Islam K, Wang QQ, Naranmandura H. Molecular Mechanisms of Arsenic Toxicity. Editor(s): James C. Fishbein, Jacqueline M. Heilman, Advances in Molecular Toxicology, Elsevier. 2015; 9: 77-107, https://doi.org/10.1016/b978-0-12-802229-0.00002-5.

Khalid A, Algarni AS, Homeida HE, Sultana S, Javed SA, Rehman ZU, Abdalla H, Alhazmi HA, Albratty M, Abdalla AN. Phytochemical, Cytotoxic, and Antimicrobial Evaluation of Tribulus terrestris L., Typha domingensis Pers., and Ricinus communis L.: Scientific Evidences for Folkloric Uses. Evidence-based complementary and alternative medicine. 2022; 6519712. https://doi.org/10.1155/2022/6519712.

Tilley SK, Fry RC. In Systems Biology in Toxicology and Environmental Health, 2015

Pivalic acid. Safety Datasheet; available from https://datasheets.scbt.com/sc-250736.pdf. Accessed on 10 Jan 2024.

Toxicological profile for 2-Butanone. US Department of Health and Human Services; Available from

https://www.ncbi.nlm.nih.gov/books/NBK590518/pdf/Bookshelf_NBK590518.pdf. Accessed on 20 Jan 2024.

Ayd?n A, Tugcu G. Toxicological assessment of epinephrine and norepinephrine by analog approach. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association. 2018; 118, 726–32.

https://doi.org/10.1016/j.fct.2018.06.028.

Piccinato CA, Montrezor LH, Collares CA, Vireque AA, Rosa e Silva AA. Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions. Reproductive biology and endocrinology. 2012; 10:95. https://doi.org/10.1186/1477-7827-10-95.

Gebrie E, Makonnen E, Zerihun L, Debella A. The possible mechanisms for the antifertility action of methanolic root extract of Rumex steudelii. Afr Health Sci. 2005; 5(2):119-25.

Alhazmi HA, Khalid A, Sultana S, Abdelwahab SI, Ahsan W, Oraiby ME, Bratty MA. Determination of Phytocomponents of Twenty-one Varieties of Smokeless Tobacco using Gas Chromatography-Mass Spectroscopy (GC-MS). S Afr J Chem. 2019, 72:47–54.

Kuivenhoven and Mason. Arsenic toxicity. National Library of Medicine; available from https://www.ncbi.nlm.nih.gov/books/NBK541125/. Accessed on 10 Jan 2024.

Oluwayinka O, Dorcas J, Ibrahim S, Binda A, Opeoluwa F, Barnabas K. Phytochemical analysis, antioxidant and anti-inflammatory potential of Feretia aponanthera root bark extracts. BMC Complementary and Alternative Medicine. 2018; 18. https://doi.org/10.1186/s12906-017-2070-z.

Rehman YU, Iqbal A, Ali G, Alotaibi G, Ahmed A, Ayaz M. Phytochemical analysis, radical scavenging and glioblastoma U87 cells toxicity studies of stem bark of buckthorn (Rhamnus pentapomica R. Parker). BMC: Complement Med Ther. 2024; 24: 12. https://doi.org/10.1186/s12906-023-04309-w.

Propanoic acid, 2, 2-dimethyl-, ethyl ester. NIST Chemistry WebBook, SRD69; available from https://webbook.nist.gov/cgi/cbook.cgi?ID=3938952&Units=SI. Accessed on 09 Jan 2024.

1,3-Dioxolane. ECHA CHEM; available from https://echa.europa.eu/registration-dossier/-/registered-dossier/15807/7/9/2. Accessed on 09 Jan 2024.

Hirsch KS, Fritz HI. Teratogenic effects of mescaline, epinephrine, and norepinephrine in the hamster. Teratology. 1981; 23(3), 287–91. https://doi.org/10.1002/tera.1420230302.

Abdullahi M, Adeniji SE. In-silico Molecular Docking and ADME/Pharmacokinetic Prediction Studies of Some Novel Carboxamide Derivatives as Anti-tubercular Agents. Chemistry Africa. 2020; 3: 989–1000. https://doi.org/10.1007/s42250-020-00162-3

Martin YC. A bioavailability score. Journal of Medicinal Chemistry. 2005; 48(9), 3164–70. https://doi.org/10.1021/jm0492002.

Lipinski CA, Lombardo F, Dominy BW, Feeney PJ. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews. 2001; 46(1-3): 3–26. https://doi.org/10.1016/s0169-409x (00)00129-0.

Daina A, Michielin O, Zoete V. SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules. Sci Rep. 2017; 3 (7): 42717. https://doi.org/10.1038/srep42717.

Jensen TK, Frederiksen H, Kyhl HB, Lassen, TH, Swan SH, Bornehag CG, Skakkebaek NE, Main KM, Lind DV, Husby S and Anderrson AM. Prenatal Exposure to Phthalates and Anogenital Distance in Male Infants from a Low-Exposed Danish Cohort (2010–2012). Environmental Health Perspectives. 2012; 124 (7), 1107 – 1113. https://doi.org/10.1289/ehp.1509870.

Hebert MF (2013). Impact of pregnancy on pharmacokinetics of medications. J Popul Ther Clin Pharmacol. 2013; 20 (3): 350-57.

Estabrook RW. A passion for P450s (rememberances of the early history of research on cytochrome P450). Drug metabolism and disposition: the biological fate of chemicals. 2003; 31:1461–1473.