This is an outdated version published on 05-09-2024. Read the most recent version.
Forthcoming

Evaluation of glucose lowering potential of Murraya paniculata plant on alloxan-induced diabetic mice

Authors

DOI:

https://doi.org/10.14719/pst.3629

Keywords:

Alloxan, antidiabetic effect, Murraya paniculata, fractionation, qualitative phytochemical screening, soxhlet

Abstract

Numerous cultures have a history of using herbal remedies to manage diabetes based on the belief that some plants possess antidiabetic properties with limited adverse effects. Murraya paniculata plant displays pharmacological properties that combat several ailments such as diabetes, bacterial infections, cancer, diarrhea and anxiety. This study aimed at phytochemical investigation and the evaluation of the blood glucose lowering impact of M. paniculata plant extracts on diabetic mice stimulated by alloxan. 85% methanolic extract of M. paniculata was fractionated using petroleum ether, chloroform and ethyl acetate. Various qualitative chemical assays were conducted to categorize different groups of chemicals in the plant extracts. An oral dose of 500 mg/kg of petroleum ether, chloroform, and ethyl acetate extracts from the whole plant of M. paniculata was given to alloxan-stimulated diabetic mice for 14 days. Blood glucose levels were measured using a glucometer and compared with non-diabetic mice, alloxan-induced diabetic mice without treatment and diabetic mice treated with metformin. The results of the phytochemical analysis detected various secondary metabolites in distinct fractions of the extract. Also, the study found no statistically significant difference in blood glucose levels among the non-diabetic group, the group treated with metformin, and those treated with chloroform or ethyl acetate extract, but the group that received petroleum ether extract treatment exhibited a notable difference compared to the other groups in the study. This study concluded that the ethyl acetate and chloroform extracts of M. paniculata effectively regulated blood glucose levels in mice with diabetes caused by alloxan.

Downloads

Download data is not yet available.

References

Kumar DPJ, Clark ML. Kumar and Klark’s clinical medicine. Tenth. Feather A, Randall D, Waterhouse M, editors. London: Elsevier; 2021. 702 p.

Tran N, Pham B, Le L. Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology (Basel). 2020;9(9):1-31. https://doi.org/10.3390/biology9090252

Sen DB, Balaraman R, Sen AK, Zanwar AS, Greeshma KP, Maheshwari RA. Anti-diabetic activity of herbal remedies. J Nat Remedies. 2023;23(2):373-81. https://doi.org/10.18311/jnr/2023/32182

Duc Van H, Thi Thao Nguyen L, Lu Chinh Phan N, Thi Thanh Dao T, Thanh Le L, Thi Ngoc Nguyen N, et al. Extract and fraction of cashew nut testa ameliorate the hyperglycemic mice induced by Streptozotocin and high-fat diet. Plant Sci Today. 2023;11(1):206-14. https://doi.org/10.14719/pst.2697

Yang T, Yin X, Kang H, Yang D, Yang X, Yang Y, et al. Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles. BMC Biol [Internet]. 2023;21(1):1-13. Available from: https://doi.org/10.1186/s12915-023-01639-6

Liu H, Zhao Y, Zhou J, Ma Q, Wang X, Hua Z. Complete chloroplast genome sequence of Murraya paniculata (Rutaceae): a widely used folk medicinal herb. Mitochondrial DNA Part B Resour. 2020;5(3):3696-97. https://doi.org/10.1080/23802359.2020.1829518

Anita Isioma Ashibuogwu, Ozadheoghene Eriarie Afieroho, Mikailu Suleiman, Kio Anthony Abo. Anti-urease and antioxidant activities of the leaf extracts from Murraya paniculata (L.) Jack (Rutaceae). GSC Adv Res Rev. 2022;11(1):156-64. https://doi.org/10.30574/gscarr.2022.11.1.0099

Joshi D, Gohil KJ. A brief review on Murraya paniculata (Orange Jasmine): Pharmacognosy, phytochemistry and ethanomedicinal uses. J Pharmacopuncture. 2023;26 (1):10-17. https://doi.org/10.3831/KPI.2023.26.1.10

Gautam MK, Gupta A, Rao CV, Goel RK. Antihyperglycemic and antioxidant potential of Murraya paniculata Linn. Leaves: a preclinical study. J Pharm Res. 2012;5(3):1334-37. https://www.researchgate.net/publication/271252398

Gautam MK, Gupta A, Vijaykumar M, Rao CV, Goel RK. Studies on the hypoglycemic effects of Murraya paniculata Linn. extract on alloxan-induced oxidative stress in diabetic and non-diabetic models. Asian Pacific J Trop Dis. 2012;2(SUPPL.1). https://doi.org/10.1016/S2222-1808 (12)60149-2

Zanzabil KZ, Hossain MS, Hasan MK. Diabetes mellitus management: An extensive review of 37 medicinal plants. Diabetology. 2023;4(2):186-234. https://www.mdpi.com/2673-4540/4/2/19#

Saxena V, Danish R, Kaushik R, Verma KK, Sharma V. A comprehensive review on natural remedies for diabetes: a metabolic disorder. J Appl Pharm Sci Res. 2022;5(3):1-16. https://doi.org/10.31069/japsr.v5i3.01.

Khamees AH, Kadhim EJ. Isolation, characterization and quantification of a pentacyclic triterpinoid compound ursolic acid in Scabiosa palaestina L. distributed in the north of Iraq. Plant Sci Today. 2022;9(1):178-82. https://doi.org/10.14719/pst.1398

Njogu SM, Arika WM, Machocho AK, Ngeranwa JJN, Njagi ENM. In vivo hypoglycemic effect of Kigelia africana (Lam): Studies with alloxan-induced diabetic mice. J Evidence-Based Integr Med. 2018;23:1-10. https://doi.org/10.1177%2F2515690X18768727

Harborne JB. Methods of plant analysis. Phytochem Methods. Third edition. 1973;1-32. https://doi.org/10.1007/978-94-009-5921-7_1

Khamees AH, Fawzi HA, Sahib HB. Phytochemical investigation and assessment of the hypoglycemic activity of two herbal extracts from selected Iraqi medicinal plants in alloxan-stimulated diabetic rats: a comparative study. F1000Research. 2020;9:247. https://doi.org/10.12688/f1000research.22788.1

Pegu J, Bawri A, Yam G, Talom D, Rochill I, Teron R. e - publishing group. Traditional remedies for diabetes used by the Ao ( Naga ) tribe in Mokochung district, Negaland, India. Plant Sci Today. 2024;11(1):381-87. https://doi.org/10.14719/pst.2602

Ibrahim RM, Abdelhafez HM, EL-Shamy SAEM, Eid FA, Mashaal A. Arabic gum ameliorates systemic modulation in alloxan monohydrate-induced diabetic rats. Sci Rep [Internet]. 2023;13(1):1-11. https://doi.org/10.1038/s41598-023-31897

CHS, GK. Evaluation of antidiabetic potential of Syzygium kanarense (Talbot) Raizada in streptozotocin- nicotinamide induced diabetic rats. Plant Sci Today. 2023;11(1):54-63. https://doi.org/10.14719/pst.2680

Queiroz LAD, Assis JB, Guimarães JPT, Sousa ESA, Milhomem AC, Sunahara KKS, et al. Endangered lymphocytes: The effects of alloxan and streptozotocin on immune cells in type 1 induced diabetes. Mediators Inflamm. 2021;2021:p.15. https://doi.org/10.1155/2021/9940009

Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216-26. https://doi.org/10.1007/s00125-007-0886

Dutta S, Shah RB, Singhal S, Bansal S, Sinha S, Haque M, et al. Metformin: A review of potential mechanism and therapeutic utility beyond diabetes. Drug Des Devel Ther. 2023;17(June):1907-32. https://doi.org/10.2147/DDDT.S409373

Menezes CDA, de Oliveira Garcia FA, de Barros Viana GS, Pinheiro PG, Felipe CFB, de Albuquerque TR, et al. Murraya paniculata (L.) (Orange Jasmine): Potential nutraceuticals with ameliorative effect in alloxan-induced diabetic rats. Phyther Res. 2017;31(11):1747-56. https://doi.org/10.1002/ptr.5903

Published

05-09-2024

Versions

How to Cite

1.
Qasim ZA, Khamees AH. Evaluation of glucose lowering potential of Murraya paniculata plant on alloxan-induced diabetic mice. Plant Sci. Today [Internet]. 2024 Sep. 5 [cited 2024 Nov. 8];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3629

Issue

Section

Research Articles