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A new species of Pythium hydnosporum as a pathogen associated with vine decline of melon (Cucumis melo L.) in Iraq

Authors

Rebwar A. Mustafa Bakrajo Technical Institute, Sulaimani Polytechnic University, Sulaimani, Iraq https://orcid.org/0000-0002-5848-1383
Samir K. Abdullah Department of Medical Laboratory Techniques, Alnoor University College, Nineva, Iraq https://orcid.org/0000-0003-3553-3571

DOI:

https://doi.org/10.14719/pst.3721

Keywords:

Cantaloupe, deterioration, fungi, melon root rot, Pythium hydnosporum

Abstract

Several pathogenic fungi have become an important vine decline of cantaloupe melone (Cucumis melo L.). In 2021, root rot on cantaloupe was observed during the harvest phase in the fields in Penjwen and Shahrazoor in Sulaimani governorate of Northern Kurdistan region Iraq. This present study was conducted to isolate the causal agent of fungi in lesions of root rot of cantaloupe. A total of 16 fungal isolates were obtained of which isolates Monosporascus cannonballus, M. eutypoides, Acremonium vitellinium, Fusarium oxysporium, F. equiseti, Macrophomina phaseolina, Saccharomyces kudriavzevii, F. robinianum, Rhizopus arrhizus, Botrytis cinerea, Cytospora eucalypticola, F. falciforme, Pythium hydnosporum, Alternaria tenuissima, Rhizoctonia solani and Phytophthora colocasiae were obtained from root melon. Identification of all fungal isolates was based on using both morphological characteristics and molecular analysis, on internal transcribed spacer (ITS1, ITS4, LSU) are primers that are used for identification. DNA sequences of the fungal pathogen were identified as Pythium hydnosporum a new pathogenic fungi causal agent of cantaloupe vine decline. A pathogenicity test was conducted to verify Koch's postulates and P. hydnosporum was observed to cause root rot of melon, symptoms of the disease were similar to those seen in the field.

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References

Saediman H, Alwi LO, Rianse IS, Taridala SA, Salahuddin S, Indarsyih Y et al. Comparative profitability of melon and watermelon production in South Konawe district of Southeast Sulawesi. WSEAS Trans Bus Econ. 2020;17:933-39. https://doi.org/10.37394/23207.2020.17.91.

Assefa AD, Hur OS, Ro Ny, Lee JE, Hwang J, Kim BS et al. Fruit morphology, citrulline and arginine levels in diverse watermelon (Citrullus lanatus) germplasm collections. Plants. 2020;9:1054. https://doi.org/10.3390/plants9091054.

Kesh H, Kaushik P. Advances in melon (Cucumis melo L.) breeding: An update. Sci Hortic. 2021;282:110045. https://doi.org/10.1016/j.scienta.2021.110045.

Nuangmek W, Aiduang W, Suwannarach N, Kumla J, Kiatsiriroat T, Lumyong S. First report of fruit rot on cantaloupe caused by Fusarium equiseti in Thailand. J Gen Plant Pathol. 2019;85:295-300. https://doi.org/10.1007/s10327-019-00841-1.

Manivannan A, Lee ES, Han K, Lee HE, Kim DS. Versatile nutraceutical potentials of watermelon—A modest fruit loaded with pharmaceutically valuable phytochemicals. Molecules. 2020;25:5258. https://doi.org/10.3390/molecules25225258.

Perkins-Veazie P, Davis A, Collins JK. Watermelon: From dessert to functional food. Isr J Plant Sci. 2012;60:395-402. https://doi.org/10.1560/IJPS.60.1.402.

Van Gastel A, MA P, Porceddu E. Seed science and technology. International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria. 1996.

Boggala V, Narute TK, Sagar SP, Akshay M. In vitro studies on susceptible reactions of groundnut varieties to macrophomina infection in relation to varied seed coat colour. Pharma Innov J. 2023;12:1245-51.

Rashid A, Fakir GA. Impact of seed health on sustainable crop production in Bangladesh. Co-Operation Yrly J Publ by Coop Dep Samabaya Sadan. 2000;24-36.

White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. In PCR Protocols. Academic Press. Inc. 1990. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal dna from several Cryptococcus species. J Bacteriol. 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990.

Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Appl Environ Microbiol. 1995;61:1323-30. https://doi.org/10.1128/aem.61.4.1323-1330.1995.

Sarpeleh A. The role of Monosporascus cannonballus in melon collapse in Iran. Australas Plant Dis Notes. 2008;3:162-64. https://doi.org/10.1071/DN08063.

Wang MM, Chen Q, Diao YZ, Duan WJ, Cai L. Fusarium incarnatum-equiseti complex from China. Persoonia-Molecular Phylogeny Evol Fungi. 2019;43:70-89. https://doi.org/10.3767/persoonia.2019.43.03.

Crous PW, Lombard L, Sandoval-Denis M, Seifert KA, Schroers HJ, Chaverri P et al. Fusarium: more than a node or a foot-shaped basal cell. Stud Mycol. 2021;98:100116. https://doi.org/10.1016/j.simyco.2021.100116.

Wang MM, Crous PW, Sandoval-Denis M, Han SL, Liu F, Liang JM et al. Fusarium and allied genera from China: Species diversity and distribution. Persoonia-Molecular Phylogeny Evol Fungi. 2022;48:1-53. https://doi.org/10.3767/persoonia.2022.48.01.

Kornerup A, Wanscher JH. Methuen handbook of colour. 3rd ed, Eyre Methuen, London, UK. 1978.

Edgar RC. MUSCLE: A multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics. 2004;5:1-19. https://doi.org/10.1186/1471-2105-5-113.

Hall A. Bioedit version 6.0.7. Available online: http://www.mbio.ncsu.edu/bioedit/bioedit.html (accessed on 20 August 2022).

Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 1985;39:783-91. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x.

Stamatakis A. RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006;22:2688-90. https://doi.org/10.1093/bioinformatics/btl446

Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES science gateway for inference of large phylogenetic trees. In: Proceedings of the 2010 Gateway Computing Environments Workshop (GCE); IEEE: New Orleans, LA, USA. 2010; pp. 1-8. https://doi.org/10.1109/GCE.2010.5676129

Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S et al. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012;61:539-42. https://doi.org/10.1093/sysbio/sys029.

Darriba D, Taboada GL, Doallo R, Posada D. JModelTest 2: More models, new heuristics and high-performance computing. Nat Methods. 2012;9:772. https://doi.org/10.1038/nmeth.2109.

Silva F AS, Azevedo CAV. de principal components analysis in the software assistat-statistical assistance. In: Proceedings of the 7th World Congress on Computers in Agriculture Conference Proceedings; American Society of Agricultural and Biological Engineers: Reno, Nevada USA. 2009.

Sneath PHA, Sokal RR. Numerical taxonomy. Freeman, San Francisco. 1973.

Bickel JT, Koehler AM. Review of Pythium species causing damping-off in corn. Plant Health Progress. 2021;22:3. https://doi.org/10.1094/PHP-02-21-0046-FI

Schmidt CS, Leclerque A, Pfeiffer T, Goessling JW, Olik M, Jamshidi B et al. Pathogenicity of Pythium species to maize. European Journal of Plant Pathology. 2020;158:335-47. https://doi.org/10.1007/s10658-020-02076-9

Schroeder KL, Martin FN, de Cock AW, Lévesque CA, Spies CF, Okubara PA et al. Molecular detection and quantification of Pythium species: Evolving taxonomy, new tools and challenges. Plant Disease. 2013;97(1):4-20. https://doi.org/10.1094/PDIS-03-12-0243-FE

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16-10-2024

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Mustafa RA, Abdullah SK. A new species of Pythium hydnosporum as a pathogen associated with vine decline of melon (Cucumis melo L.) in Iraq. Plant Sci. Today [Internet]. 2024 Oct. 16 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/3721

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