Phytochemical screening and in vitro evaluation of alpha amylase, alpha glucosidase and beta galactosidase inhibition by aqueous and organic Atractylis gummifera L. extracts
Laboratory of Materials, Natural Substances, Environment and Modeling (LMSNEM), Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University of Fez B.P.: 1223 Taza-Gare. Taza, Morocco
Laboratory of Materials, Natural Substances, Environment and Modeling (LMSNEM), Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University of Fez B.P.: 1223 Taza-Gare. Taza, Morocco
Professor at the Polydisciplinary Faculty of Taza, University Sidi Mohammed Ben Abdellah of Fez since 2003 ; Deputy Head of Biology, Chemistry and Geology Department (BCG) in 2010-2011 and 2011-2012 ; Head of Department BCG during academic year 2012 - 2013, Head of Die Life Sciences since 2009 and Director of the Laboratory of Materials, Natural Substances, Environment and Modeling (LMSNEM) since 2014.
Research interests: Medicinal plants, Natural Substances, Phytochemistry, Research pharmacological activities ; Toxicity studies in vitro and in vivo models, experimental oncology, molecular modeling and Biosafety, Biosecurity and Dual-Use.
Major achievements: I conducted research on the medicinal plant Peganum harmala from screening tests using aqueous extracts to the isolation and identification of pure active principles of this plant and the results have been published in several publications. Currently, I am working on other plants, including a Moroccan endemic plants.
Number peer reviewed publications: 23 publications from 1998 to 2014
Memberships, etc : Director of the Laboratory of Materials, Natural Substances, Environment and Modeling (LMSNEM) since 2014, Head of the Research Team "Natural Substances and Environment" since 2009; Member of the executive bureau of the Moroccan Biosafety Association (MABS) as Assistant Treasurer since its inception in 2009 ; Vice President of the Moroccan Society of Medicinal Chemistry’s Polydisciplinary Faculty of Taza Division; Referred scientific articles (Reviewer) in : Arabian Journal of Chemistry , Journal of Applied Polymer Science, Journal of Medicinal Plants Research and Physical, Journal of Materials and Environmental Science, and Chemical News Journal.
Laboratory of Materials, Natural Substances, Environment and Modeling (LMSNEM), Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University of Fez B.P.: 1223 Taza-Gare. Taza, Morocco
Laboratory of Pharmacology and Toxicology, Pharmacokinetics Research Team, Faculty of Medicine and Pharmacy, Mohammed V University, BP 6203, Rabat-Institutes, Rabat, Morocco
Laboratory of Pharmacology and Toxicology, Pharmacokinetics Research Team, Faculty of Medicine and Pharmacy, Mohammed V University, BP 6203, Rabat-Institutes, Rabat, Morocco
Laboratory of Pharmacology and Toxicology, Pharmacokinetics Research Team, Faculty of Medicine and Pharmacy, Mohammed V University, BP 6203, Rabat-Institutes, Rabat, Morocco
Diabetes is a chronic condition which is increasingly progressing throughout the world. To treat it, several methods are used, among which is medicinal plants that still have an unknown mechanism of action. The objective of this work is to evaluate the in vitro hypoglycemic effect of the extracts of the underground part of Atractylis gummifera, a member of Asteraceae used in traditional Moroccan medicine. A phytochemical study of the aqueous extracts (decocted, infused and macerated) and organic extracts (methanol, methanol macerate, chloroformic, ethyl acetate and petroleum ether), and a phytochemical screening of the different secondary metabolites was done. The antidiabetic power of the extracts of A. gummifera by testing the inhibitory activity of ?-amylase, ?-glucosidase and ?-galactosidase, which are enzymes responsible for the digestion of polysaccharides was determined. The extracts of A. gummifera are very rich in flavonoids and tannins, and are inhibitory to?-amylase and ?-glucosidase, mainly the macerate of methanol with IC50 values of 0.557 ± 0.013 and 0.743 ± 0.017 mg / mL respectively. Higher ?-galactosidase inhibitory potential than quercetin was observed for aqueous macerates and methanol with IC50 values of 2.23 ± 0.012 and 2.443 ± 0.071 mg / mL respectively. The extracts of A. gummifera possess a significant inhibitory activity of the alpha amylase and alpha glucosidase and beta-galactosidase enzymes, in particular the macerate of methanol followed by the aqueous macerate, among the eight extracts tested.
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