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Plant Science Today (PST)

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Early Access

Evaluation of the content of polyphenols, flavonoids, tannins, antioxidant capacity and antibacterial activity of Pelargonium graveolens flower extracts

DOI
https://doi.org/10.14719/pst.3975
Submitted
25 May 2024
Published
22-01-2025
Versions

Abstract

Pelargonium graveolens has garnered attention for its therapeutic properties, especially in treating dermatological conditions. The aim of this study was to investigate the phytochemicals, antioxidant action and antibacterial effect of its flower extracts obtained through maceration, sonication and infusion. The findings show that the highest total phenolic content was obtained from the hydroethanolic sonication extract (280.39 mg GAE/g), followed by the hydroethanolic maceration extracts (272.87 mg GAE/g). The total flavonoid content varied from 21.51 mg EQ/g (hydroethanolic maceration) to 12.32 mg EQ/g (aqueous infusion). Condensed tannins were highest in the hydroethanolic maceration extracts (8.40 mg/mL). Additionally, the antioxidant action was assessed using the DPPH, FRAP, TAC and ABTS methods. The extracts from hydroethanolic sonication showed a high free radical scavenging capacity (IC50 = 0.31 mg/mL) compared with extracts from aqueous infusion and hydroethanolic maceration. However, antibacterial tests revealed that all extracts had better activity against Gram-positive bacteria. The hydroethanolic maceration extracts showed pronounced sensitivity, exhibiting zones of inhibition ranging from 13 to 24 mm for Gram-negative bacteria and 19 to 24 mm for Gram-positive bacteria. These extracts demonstrated significant efficacy against methicillin-resistant Staphylococcus aureus and Panton-Valentine Leukocidin-Positive Staphylococcus aureus. These findings suggest potential applications in the pharmaceutical and dermatological fields, particularly for developing natural antioxidant and antibacterial agents.

References

  1. Hassani L, Sahel H. Enquête sur l'activité de consultations en dermatologie entre le secteur public et privé. [ Survey of dermatology consultations between the public and private sectors]. Doctoral Thesis in Medicine, Faculty of Medicine in Constantine 3, Salah Boubnider University. 2023. https://dspace.univ-constantine3.dz/jspui/bitstream/123456789/5372/1/THESE%20Dr%20HASSANI.pdf
  2. Dziri S, Azzabi A, Tlili G, Sahtout W, Azouz M, Aicha NB, et al. Dermatological conditions seen in kidney transplant recipients: prevalence and risk factors. Exp Clin Transplant. 2024;22(Suppl 1):310-14. https://doi.org/10.6002/ect.MESOT2023.P108
  3. Mouad S. Infections bacteriennes cutanees: epidemiologie et nouvelles recommandations de prise en charge. [Bacterial skin infections: epidemiology and new management recommendations]. Doctoral Thesis in Medicine, M1472021, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat. 2021. https://toubkal.imist.ma/handle/123456789/29956
  4. Elachouri M, Kharchoufa L, Fakchich J, Lorigooini Z, Subhasis P, Subhash M. Ancestral phytotherapeutic practices in Morocco: regards on history, current state, regulatory and safety of commonly used herbal medicine. Arabian Journal of Chemical and Environmental Research. 2021;8(1):133-49. https://www.mocedes.org/ajcer/volume8/AJCER-09-Elachouri-2021.pdf
  5. Radi FZ, Khamar H, Remok F, Amine S, Drioiche A, Aoudry S, et al. Aromatic and medicinal plants in the Ifrane region (Morocco): Floristic diversity, with special emphasis on ethnobotanical and socioeconomic studies of six spontaneous plants. J Pharm Pharmacogn Res. 2022;10(3):517-50. https://doi.org/10.56499/jppres21.1331_10.3.517
  6. Salem AB, Boujraf A, Dallahi Y, El Aboudi A. Contribution to the evaluation of the biomass of medicinal and aromatic plants in Morocco: Case of Globularia alypum. Biosystems Diversity. 2023;31(4):535-41. https://doi.org/10.15421/012363
  7. Hiben MG. Trends of traditional medicine research and drug discovery: A review. Int J Pharmacogn. 2023;11(2):534-53. https://doi.org/10.13040/IJPSR.0975-8232.IJP.10(11).534-53
  8. Anand U, Jacobo-Herrera N, Altemimi A, Lakhssassi N. A comprehensive review on medicinal plants as antimicrobial therapeutics: potential avenues of biocompatible drug discovery. Metabolites. 2019;9(11):258. https://doi.org/10.3390/metabo9110258
  9. Dubey N, Kumar R, Tripathi P. Global promotion of herbal medicine: India's opportunity. Curr Sci. 2004;86(1):37-41. https://www.jstor.org/stable/24109515
  10. Miller DM. The taxonomy of Pelargonium species and cultivars, their origins and growth in the wild. Geranium and Pelargonium: CRC Press. 2002; p. 61-91. https://www.taylorfrancis.com/chapters/edit/10.1201/9780203216538-13/taxonomy-pelargonium-species.
  11. Xu Z, Deng M. Geraniaceae. In: Identification and Control of Common Weeds: Volume 2. Springer, Dordrecht; 2017. https://doi.org/10.1007/978-94-024-1157-7_45
  12. Chraibi M, Fikri-Benbrahim K, Amrani M, Farah A, Bari A, Ouaritini ZB. Etude éthnobotanique sur l’utilisation de Mentha pulegium, Mentha piperita et Pelargonium graveolens au nord du Maroc (Taounate) et évaluation de leur pouvoir antimicrobien. [Ethnobotanical study on the use of Mentha pulegium, Mentha piperita and Pelargonium graveolens in northern Morocco (Taounate) and evaluation of their antimicrobial properties]. Eur Sci J. 2018;14(24):113-33. https://doi.org/ 10.19044/esj.2018.v14n24p114
  13. Asgarpanah J, Ramezanloo F. An overview on phytopharmacology of Pelargonium graveolens L. Indian J Tradit Knowl. 2015;14(4):558-63. https://api.semanticscholar.org/CorpusID:55701384
  14. Lister E, Wilson P. Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). Crop Research Institute, Lincoln, New Zealand. 2001;7:235-59. https://doi.org/ 10.1016/j.apjtm.2017.07.024
  15. Moutawalli A, Benkhouili FZ, Ouchari L, El Fahime E, Benzeid H, Doukkali A, Zahidi A. Quantitative phytochemical, antioxidant and antimicrobial properties of the seeds of Lawsonia inermis L. Plant Science Today. 2024;11(2):105-16. https://doi.org/10.14719/pst.2834
  16. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry. 2006;97(4):654-60. https://doi.org/ 10.1016/j.foodchem.2005.04.028
  17. RibéreauGayon P, Stonestreet E. Dosage des tanins du vin rouge et détermination de leur structure. [Determination of red wine tannins and their structure]. Chimie Analytique. [Analytical Chemistry]. 1966;48(4):188-96.
  18. Topçu G, Ay M, Bilici A, Sar?kürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chemistry. 2007;103(3):816-22. https://doi.org/ 10.1016/j.foodchem.2006.09.028
  19. Ait Bouzid H, Sakar EH, Bijla L, Ibourki M, Zeroual A, Gagour J, et al. Physical fruit traits, proximate composition, antioxidant activity and profiling of fatty acids and minerals of wild Jujube (Ziziphus lotus L.(Desf.)) fruits from eleven Moroccan origins. J Food Qual. 2022;14:1-15. https://doi.org/10.1155/2022/9362366
  20. Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet. 1986;44(6):307-15. https://doi.org/10.5264/eiyogakuzashi.44.307
  21. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry. 1999;269(2):337-41. https://doi.org/10.1006/abio.1999.4019
  22. Jenkins SG, Schuetz AN, editors. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clinic Proceedings. Elsevier. 2012. https://doi.org/ 10.1016/j.mayocp.2012.01.007
  23. Al-Mijalli SH, Mrabti HN, Assaggaf H, Attar AA, Hamed M, Baaboua AE, et al. Chemical profiling and biological activities of pelargonium graveolens essential oils at three different phenological stages. Plants. 2022;11(17):2226. https://doi.org/10.3390/plants11172226
  24. Bouyahya A, Belmehdi O, El Jemli M, Marmouzi I, Bourais I, Abrini J, et al. Chemical variability of Centaurium erythraea essential oils at three developmental stages and investigation of their in vitro antioxidant, antidiabetic, dermatoprotective and antibacterial activities. Industrial Crops and Products. 2019;132:111-17. https://doi.org/10.1016/j.indcrop.2019.01.042
  25. El Aanachi S, Gali L, Nacer SN, Bensouici C, Dari K, Aassila H. Phenolic contents and in vitro investigation of the antioxidant, enzyme inhibitory, photoprotective and antimicrobial effects of the organic extracts of Pelargonium graveolens growing in Morocco. Biocatal Agric Biotechnol. 2020;29:101819. https://doi.org/10.1016/j.bcab.2020.101819
  26. Raghunath S, Budaraju S, Gharibzahedi SMT, Koubaa M, Roohinejad S, Mallikarjunan K. Processing technologies for the extraction of value-added bioactive compounds from tea. Food Engineering Reviews. 2023;1-33. https://doi.org/10.1007/s12393-023-09338-2
  27. Su M, Jin R, Zhu J, Pei J, Chai X, Wang Y, et al. Investigation of the microstructure and dynamical evolution following gradient hydration of deep eutectic solvent, with application to polyphenol extraction. ACS Sustain Chem Eng. 2024. https://doi.org/10.1021/acssuschemeng.3c05709
  28. Chebil L, Humeau C, Anthoni J, Dehez F, Engasser J-M, Ghoul M. Solubility of flavonoids in organic solvents. J Chem Eng Data. 2007;52(5):1552-56. https://doi.org/10.1021/je7001094
  29. Xiao J. Dietary flavonoid aglycones and their glycosides: Which show better biological significance?. Crit Rev Food Sci Nutr. 2017;57(9):1874-905. https://doi.org/10.1080/10408398.2015.1032400
  30. Tsao R, McCallum J. Chemistry of flavonoids. In: Fruit and Vegetable Phytochemicals. Blackwell Publishing. 2010;131-53. https://doi.org/10.1002/9780813809397.ch5
  31. Kahkeshani N, Farzaei F, Fotouhi M, Alavi SS, Bahramsoltani R, Naseri R, et al. Pharmacological effects of gallic acid in health and diseases: A mechanistic review. Iranian Journal of Basic Medical Sciences. 2019;22(3):225. https://doi.org/10.22038/ijbms.2019.32806.7897
  32. Ghareib HRA, Abdelhamed MS, Ibrahim OH. Antioxidative effects of the acetone fraction and vanillic acid from Chenopodium murale on tomato plants. Weed Biol Manag. 2010;10(1):64-72. https://doi.org/10.1111/j.1445-6664.2010.00368.x
  33. Kashyap D, Sharma A, Tuli HS, Sak K, Punia S, Mukherjee TK. Kaempferol–A dietary anticancer molecule with multiple mechanisms of action: Recent trends and advancements. J Funct Foods. 2017;30:203-19. https://doi.org/10.1016/j.jff.2017.01.022
  34. Buranasudja V, Muangnoi C, Sanookpan K, Halim H, Sritularak B, Rojsitthisak P. Eriodictyol attenuates H2O2-Induced oxidative damage in human dermal fibroblasts through enhanced capacity of antioxidant machinery. Nutrients. 2022;14(12):2553. https://doi.org/10.3390/nu14122553
  35. Leal Filipe P. Sur les mécanismes moléculaires de l'action des flavonoïdes lors d'un stress oxydant ou photooxydant. [On the molecular mechanisms of flavonoid action during oxidative or photooxidative stress]. Doctoral Thesis, Paris 7. 2005. https://theses.fr/2005PA077204
  36. Zhao W, Subbiah V, Xie C, Yang Z, Shi L, Barrow C, et al. Bioaccessibility and bioavailability of phenolic compounds in seaweed. Food Reviews International. 2023;39(8):5729-60. https://doi.org/10.1080/87559129.2022.2094404
  37. M’Hiri N. Étude comparative de l’effet des méthodes d’extraction sur les phénols et l’activité antioxydante des extraits des écorces de l’orange «Maltaise demi sanguine» et exploration de l’effet inhibiteur de la corrosion de l’acier au carbone.[Comparative study of the effect of extraction methods on the phenols and antioxidant activity of extracts from the peel of the "Maltese half-blood" orange and exploration of the corrosion-inhibiting effect on carbon steel]. Doctoral Thesis, INAT, Tunis. 2015. http://docnum.univ-lorraine.fr/public/DDOC_T_2015_0183_M_HIRI.pdf
  38. Brook I. Microbiology of polymicrobial abscesses and implications for therapy. J Antimicrob Chemother. 2002;50(6):805-10. https://doi.org/10.1093/jac/dkg009
  39. Percival SL, Emanuel C, Cutting KF, Williams DW. Microbiology of the skin and the role of biofilms in infection. Int Wound J. 2012;9(1):14-32. https://doi.org/10.1111/j.1742-481X.2011.00836.x
  40. Kumari S, Harjai K, Chhibber S. Efficacy of bacteriophage treatment in murine burn wound infection induced by Klebsiella pneumoniae. J Microbiol Biotechnol. 2009;19(6):622-28. https://doi.org/10.4014/jmb.0808.493
  41. Krapp F, Morris AR, Ozer EA, Hauser AR. Virulence characteristics of carbapenem-resistant Klebsiella pneumoniae strains from patients with necrotizing skin and soft tissue infections. Sci Rep. 2017;7(1):1-14. https://doi.org/10.1038/s41598-017-13524-8
  42. Moreira M, Ponce A, Del Valle C, Roura S. Inhibitory parameters of essential oils to reduce a foodborne pathogen. LWT-Food Science and Technology. 2005;38(5):565-70. https://doi.org/10.1016/j.lwt.2004.07.012
  43. Miklasi?ska-Majdanik M, K?pa M, Wojtyczka RD, Idzik D, W?sik TJ. Phenolic compounds diminish antibiotic resistance of Staphylococcus aureus clinical strains. Int J Environ Res Public Health. 2018;15(10):2321. https://doi.org/10.3390/ijerph15102321

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