Changes in amino acid levels and their effects on parthenocarpic fruit formation in young Barhi date palms Phoenix dactylifera L. derived from tissue culture

A. Ghailan  Salah; A. Suhim  Aqeel; M. Awad Khairullah

doi:10.14719/pst.3982

Authors

A. Ghailan Salah Department of Horticulture and landscape, College of Agriculture, University of Basrah, Basrah 61004, Iraq https://orcid.org/0009-0007-8356-5101
A. Suhim Aqeel Department of Horticulture and landscape, College of Agriculture, University of Basrah, Basrah 61004, Iraq https://orcid.org/0000-0002-3824-3097
M. Awad Khairullah Date palm research Centre, University of Basrah, Basrah 61004, Iraq https://orcid.org/0000-0003-2018-6474

DOI:

https://doi.org/10.14719/pst.3982

Keywords:

essential amino acids, HPLC analysis, flowering, phenotypes, methionine

Abstract

This research aims to analyse the quantities of non-essential amino acids (histidine, arginine, alanine, cysteine and tyrosine) and essential amino acids (aspartic, glutamine, serine, glycine, phenylalanine, threonine, valine, isoleucine, leucine, methionine and lysine) in the leaves of three different date palm phenotypes. These phenotypes are derived through tissue culture from and parthenocarpic fruits (shees) produces, normal fruits (normal) produce and propagated by offshoots of normal fruits (vegetal) produces. This study focuses on three distinct stages: pre-flowering, flowering and fruiting. The amino acid levels were determined using HPLC. Results of this study indicate that the shees phenotype has the lowest levels of amino acids compared with the normal and vegetal phenotypes. Most amino acids exhibit a consistent trend throughout the examined stages, with levels declining from the pre-flowering stage to the flowering stage, but show an increase in the fruiting stage compared with the preceding stage. In addition, the results reveal that methionine is absent in the shees phenotype during all stages of the study but present at high levels in the vegetal and normal phenotypes. These results indicate that the metabolism of amino acids varies among different phenotypes of date palms. This variation directly or indirectly affects the development of parthenocarpic fruits in date palms of the Barhi cultivar derived from tissue culture during the juvenile period.

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