Direct shoot regeneration from male immature flower buds of Musa paradisiaca Linn. cv. Poovan (AAB)
DOI:
https://doi.org/10.14719/pst.2018.5.4.403Keywords:
Banana, inflorescence, Immature male flower buds, Micropropagation, Musa, PoovanAbstract
A tissue culture system has been developed to multiply Musa paradisiaca cv. Poovan using male immature flower bud and to establish it in ex vitro condition. Size of explants has been found an influencing factor for culture initiation. Immature male flower bud segments of 3 cm size were ideal for better survival and subsequent shoot regeneration. Direct shoot regeneration was achieved from male immature flower buds on Murashige and Skoog (MS) medium supplemented with varying concentrations of plant growth regulators. Initially, actively dividing meristematic region developed at the basal region of flower buds near the bract axil, which later grew into green shoot buds in most of the PGR treatments. Single use of benzyl adenine were found beneficial than kinetin or addition of indole-3-acetic acid. Maximum production of 31.0 ± 0.65 shoots was achieved on MS + 3% sucrose + 6 mg/L benzyl adenine in 15 weeks. Isolated healthy shoots were rooted in half-strength MS medium with 150 mg/L activated charcoal + 30 g/L sucrose + 1 mg/L indole-3-butyric acid within 15 days and they established successfully in greenhouse conditions with 85 % survival.
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2. Nair ARG, Ravichandran P, Bejoy M. Paclobutrazol Mediated Enhanced Multiplication of Musa Paradisiaca L. cv. Poovan (AAB). Int. J. Adv. Res. 2017; 5: 2320-5407. https://doi.org/10.21474/IJAR01/4462
3. Rao SNK, Chacko EK, DoreSwamy R, Narayanasamy S. Induction of growth in explanted infloresence axis of banana. Curr. Sci. 1982; 51:666-667.
4. Cronauer SS, Krikorian AD. Reinitiation of vegetative growth from aseptically cultured terminal floral apex of bananas. American Journal of Botany. 1985; 7: 23-25. https://doi.org/10.1002/j.1537-2197.1985.tb08423.x
5. Doreswamy R, Shajiram L. Micropropagation of banana from male floral apices cultured in vitro. Scientia Horticulturae. 1989; 40: 181-188. https://doi.org/10.1016/0304-4238(89)90111-8
6. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
7. Smitha PD, Binoy KR, Nair AS. Effect of TDZ on Direct Shoot Regeneration from Whole Male Inflorescence of Four Diploid Banana Cultivars from South India, Plant Science International. 2014;1: 24-32. https://doi.org/10.12735/psi.v1n1p24
8. Resmi L, Nair AS. Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India. Plant Cell Tiss Org Cult. 2007; 88: 333-338. https://doi.org/10.1007/s11240-007-9206-7
9. Krikorian AD, Irizarry H, Cronauer SS, Rivera E. Clonal fidelity and variation in plantain (Musa spp.) regenerated from vegetative stem and floral axis tips in vitro. Ann Bot. 1993; 71: 519-535. https://doi.org/10.1006/anbo.1993.1068
10. Israeli Y, Lahav E, Reuveni O. In vitro culture of bananas. In: Bananas and plantains. Chapman and Hall, London; 1995. p. 147-178. https://doi.org/10.1007/978-94-011-0737-2_6
11. Mendes BMJ, Flippi SB, Dementrio GB, Rodriguez AP. A statistical approach to study the dynamics of Micro propagation rate using banana (Musa spp) as example. PI. Cell Rep. 1999; 18: 967-971. https://doi.org/10.1007/s002990050692
12. Darvari FM, Sariah M, Puad MP, Maziah M. Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African Journal of Biotechnology. 2010; 9: 2360 - 2366.
13. Banerjee N, De Langhe. A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and Plantain). Plant Cell Reports. 1985; 4: 351-354. https://doi.org/10.1007/BF00269897
14. Strosse H, Van den Hauwe I, Panis B. Banana cell and Tissue culture review. In: Jain, SM & Swennen, R (eds). Banana Improvement; Cellular, Molecular Biology and Induced Mutations. 2004.
15. Nair ARG, Ravichandran P, Bejoy M. In vitro shoot regeneration of Musa Paradisiaca cv. Poovan (AAB) from bract derived calli. Abstracts of the National Seminar, 17th-21st Feb 2018, National Banana Festival, Thiruvananthapuram; 2018. p. 48.
16. Cronauer SS, Krikorian AD. Rapid multiplication of bananas and plantains by in vitro shoot-tip culture. Horti Sci. 1984; 19: 234-235.
17. Jarret RL, Fisher JB, Litz RE Organ formation in Musa tissue cultures. Plant Physiol. 1985; 121:123-130. https://doi.org/10.1016/S0176-1617(85)80037-7
18. Asnita AH, Norzulaani K. Direct regeneration and RAPD assessment of male inflorescence derived plants of Musa acuminata cv. Berangan. Asia Pac. J. Mol. Biol. Biotechnol. 2006; 14: 11-17.
19. Arnaitwe G, Rubaihayo PR, Magambo MJS. Proliferation rate effects of cytokinins on banana (Musa spp.) cultivars. Sci Hort. 2000; 86: 13-21. https://doi.org/10.1016/S0304-4238(00)00124-2
20. Banerjee N, Vuylsteke D, De Langhe EAL. Meristem tip culture of Musa, histo morphological studies of shoot bud proliferation. In Withers, L. A. and Giderson, P., eds. Plant Tissue Culture and its Agricultural Applications, Butterworth, London; 1986. p. 139-147. https://doi.org/10.1016/B978-0-407-00921-9.50019-1
21. Mukunthakumar S, Praveen G, Seeni S. Rapid propagation of two seedless diploid (AB) land races of Musa through in vitro culture of shoot and inflorescence apices. Tree For. Sci. Biotechnol. 2010; 4: 64-68.
22. Hirimburegama K, Gamage N. Cultivar specificity to in vitro micropropagation of Musa spp. (banana and plantain). J Hort Sci. 1997; 72: 205–211. https://doi.org/10.1080/14620316.1997.11515507
23. Gubbuk H, Pekmezci M. In vitro Propagation of Some New Banana Types (Musa spp.). Turkish Journal of Agriculture and Forestry. 2004; 28: 355-361.
24. Sultan MT, Khan MH, Hakim ML, Mamun ANK, Morshed MA, Islam MR, Islam MR. In vitro plant regeneration from male flowers of banana. IJB.2011; 1:1-11.
25. Radhika BJ, Sreekumaran MK, Bejoy M. In vitro regeneration studies in Musa acuminata cultivar CV Rose (AA) using inflorescence apices. Abstracts of the National Seminar, 17th-21st Feb 2018, National Banana Festival, Thiruvananthapuram; 2018. p. 49.
26. Bhaskar J, Aravindakchan M, Balsalakumari PK, Rajeevan PK. Micropropagation studies in banana. South Indian Hort. 1993; 41:186-191.
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