Exploring the potential of bioactive compounds of Capparis spinosa L. from Morocco: Unveiling its antioxidant and antifungal powers against Alternaria alternata
DOI:
https://doi.org/10.14719/pst.4074Keywords:
Capparis spinosa L., polyphenols, flavonoids, DPPH, ABTS, Alternaria alternataAbstract
Capparis spinosa L. is a Mediterranean shrub and one of the most important species of the gender Capparis due to its ecological, medicinal and economic importance. This species has strong adaptation characteristics to regions with fluctuating climates. This work aims to evaluate the effect of the locality’s climate and plant’s parts on the content of polyphenols, flavonoids and antioxidant and antifungal activities of caper leaves and seeds. The present study was carried out in 7 localities in 2 Moroccan regions, Oriental and Fez-Meknes, where we collected leaves and seeds samples. Phenolic compounds were determined by the Folin Ciocalteu method, antioxidant activity was studied by DPPH and ABTS tests and an in vitro test of antifungal activity was achieved using Alternaria alternata. The analysis of variance showed no significant effect of locality, plant’s parts and their interaction on the phenolic compounds, antioxidant and antifungal activities of C. spinosa. Methanolic leaves extracts demonstrated the most favorable outcomes, yielding a maximum polyphenol content of 124.48 ± 0.05 mg EAG/g DW. Additionally, the maximum flavonoid content was recorded at 24.51 ± 0.01 mg EQ/g DW. The evaluation of the antioxidant activity showed that the minimum inhibitory concentration at 50 % (IC50) is 2.06 ± 0.05 mg/mL and 2.05 ± 0.02 mg/mL using DPPH and ABTS tests respectively. These extracts exhibited the highest % of inhibition, 30.59 % at 1000 ppm against A. alternata. The richness of the caper plant in bioactive compounds reveals an interest in its therapeutic and pharmaceutical virtues. In addition, it could be an alternative to chemicals for the control of phytopathogenic fungi on fruits or vegetables.
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Locatelli C, Melucci D, Locatelli M. Toxic metals in herbal medicines. A review. Curr BioactCompd. 2014;10(3):1818. https://doi.org/10.2174/1573407210666140716164321
Chiboub B, Maatougui A, Aboukhalid K, Otouya S, Zarqi F, Nazih A, et al. Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L. J Appl Nat Sci. 2024;16(1):126. https://doi.org/10.31018/jans.v16i1.5287
Zhang H, Ma Z. Phytochemical and pharmacological properties of Capparis spinosa as a medicinal plant. Nutrients. 2018;10(2):116. https://doi.org/10.3390/nu10020116
Sun Y, Yang T, Wang C. Capparis spinosa L. as a potential source of nutrition and its health benefits in foods: A comprehensive review of its phytochemistry, bioactivities, safety and application. Food Chem. 2023;30;409:135258.
https://doi.org/2023;409:135258. 10.1016/j.foodchem.2022.135258
Fici S. Intraspecific variation and evolutionary trends in Capparis spinosa L. (Capparaceae). Plant Syst Evol. 2001;228(34):12341.
https://doi.org/10.1007/s006060170024
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 1999;299:15278. https://doi.org/10.1016/S0076-6879(99)99017-1
Ali-Rachedi F, Meraghni S, Touaibia N, Mesbah S. Analyse quantitative des composés phénoliques d’une endémique algérienne Scabiosa atropurpurea sub. maritima L. Bull Société R Sci Liège. 2018;1321. https://doi.org/10.25518/0037-9565.7398
Itidel C, Chokri M, Mohamed B, Yosr Z. Antioxidant activity, total phenolic and flavonoid content variation among Tunisian natural populations of Rhus tripartita (Ucria) Grande and Rhus pentaphylla Desf. Ind Crops Prod. 2013;51:1717.
https://doi.org/10.1007/s00468-021-02262-0
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(910):12317. https://doi.org/10.1016/s0891-5849(98)00315-3
Singh J, Tripathi NN. Inhibition of storage fungi of blackgram (Vinga mungo L.) by some essential oils. Flavour fragr J. 1999;14(1):1-4. http.//doi.org/10.1002/(SICI)1099-1026(199901/02)14:1<1::AID-FFJ735>3.0.CO:2-R
Ashraf U, Chaudhry MN, Ahmad SR, Ashraf I, Arslan M, Noor H, et al. Impacts of climate change on Capparis spinosa L. based on ecological niche modeling. PeerJ. 2018;6:e5792. https://doi.org/10.7717/peerj.5792
Arena A, Bisignano G, Pavone B, Tomaino A, Bonina FP, Saija A, et al. Antiviral and immunomodulatory effect of a lyophilized extract of Capparis spinosa L. buds. Phytother Res. 2008;22(3):3137. https://doi.org/10.1002/ptr.2313
Arrar L, Benzidane N, Krache I, Charef N, Khennouf S, Baghiani A. Comparison between polyphenol contents and antioxidant activities of different parts of Capparis spinosa L. Pharmacogn Commun. 2013;3(2):704. https://doi.org/10.5530/pc.2013.2.14
Akkari H, B’chir F, Hajaji S, Rekik M, Sebai E, Hamza H, et al. Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity. Veterinární Medicína. 2016;61(6):30816.
https://doi.org/10.5923/j.ajmms.20120201.06
El Finou H, Salhi N, Zaid A, El Rhaffari L. Phytotoxicity, antioxidant activity and chemical profile of aqueous extracts from Moroccan caper (Capparis spinosa L.). Sci Afr. 2024;24:e02176. https://doi.org/10.1016/j.sciaf.2024.e02176
Rajhi I, Ben Dhia MT, Abderrabba M, Ouzari-Hadda I, Ayadi S. Phytochemical screening, in vitro antioxidant and antibacterial activities of methanolic extracts of Capparis spinosa L. different parts from Tunisia. Journal of Materials Environ.
;10(3):23443. ISSN:2028;2508
Meddour A, Yahia M, Benkiki N, Ayachi A. Étude de l’activité antioxydante et antibactérienne des extraits d’un ensemble des parties de la fleur du Capparis spinosa L. Leban Sci J. 2013;14(1). https://doi.org/10.1016/j.fitote.2010.09.006
Tagnaout I, Zerkani H, Mahjoubi M, Bourakhouadar M, Alistiqsa F, Bouzoubaa A, et al. Phytochemical study, antibacterial and antioxidant activities of extracts of Capparis spinosa L. International J Pharmacogn Phytochem Res. 2016;8(12):19932005. ISSN:
-4873
Zamani E, Kamali Allabad K, Kalantar S, Sodaeizadeh H, Haghiralsadat B, Capanoglu E. Investigating the effect of the habitat and type of extracted organ on the phytochemical compounds of caper (Capparis spinosa L.) as a medicinal and pasture
plant. Iranian Scientific Association of Desert Management and Control (ISADMC). 2023;5772.
Williams RJ, Spencer JPE, Rice-Evans C. Flavonoids: antioxidants or signaling molecules? Free Radic Biol Med. 2004;36(7):83849. https://doi.org/10.1016/j.freeradbiomed.2004.01.001
Merghache D, Boucherit-Otmani Z, Merghache S, Chikhi I, Selles C, Boucherit K. Chemical composition, antibacterial, antifungal and antioxidant activities of Algerian Eryngium tricuspidatum L. essential oil. Nat Prod Res. 2014;28(11):795807.
https://doi.org/10.1080/14786419.2014.883392
Turkmen N, Velioglu YS, Sari F, Polat G. Effect of extraction conditions on measured total polyphenol contents and antioxidant and antibacterial activities of black tea. Molecules. 2007;12(3):48496. https://doi.org/10.3390/12030484
El-Bakkosh AM, M Shaieb F, M Idrs A. Phytotoxicity and antifungal activity of Capparis spinosa L. EPH - Int J Appl Sci. 2020;6(1):611.
https://doi.org/10.1016/j.freeradbiomed.2004.01.001
Al-Askar AAA. In vitro antifungal activity of three Saudi plant extracts against some Phytopathogenic fungi. J Plant Prot Res. 2012;52(4):45862. https://doi.org/10.1080/14786419.2014.883392
Arash Mahboubi. Evaluation of antibacterial activity of three Iranian medicinal plants. Afr J Microbiol Res. 2012;6(9):2048-52. https://doi.org/10.5897/AJMR11.1281
Tlili N, Khaldi A, Triki S, Munné-Bosch S. Phenolic compounds and vitamin antioxidants of caper (Capparis spinosa). Plant Foods Hum Nutr. 2010;65(3):2605. https://doi.org/10.1007/s11130-010-0180-6
Ouhammou M, Adnany EME, Mourjane A, Hammou HA, Bouchdoug M, Jaouad A, et al. Physico-chemical analysis and antioxidant activity of Moroccan caper leaves (Capparis Spinosa L.). Euro-Mediterr J Environ Integr. 2022;7(3):40714.
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Copyright (c) 2024 Basma Chiboub, Abdesselam Maatougui, Kaoutar Aboukhalid, Abdelhak Khallou, Hanane Khallouf, Abderrahmane Nazih, Loubna Esseghir, Mourad Baghour
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