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Impact of exogenous ascorbic acid and putrescine on vegetative, root system morphology and chemical composition of clementine Mandarin saplings

Authors

DOI:

https://doi.org/10.14719/pst.4075

Keywords:

Antioxidants, foliar spray, polyamines, putrescine, root growth, vegetative system

Abstract

Spraying with antioxidants and polyamines is one of the techniques that contribute to improving vegetative growth and the architectural structure of the roots, in addition to improving the chemical content of the plant. The objective of the study was to enhance the traits related to vegetative growth, root development, and chemical composition of one-year-old Clementine mandarin saplings. This was achieved by applying ascorbic acid through foliar application at different concentrations (0,300, 600, 900 mg/L) and utilizing three concentrations of putrescine (0,75,150 mg/L). The results showed that most of the growth indicators in terms of main shoot number, main shoot length, vegetative dry weight, taproot length, secondary lateral roots number, secondary lateral roots length, roots system dry weight, chlorophyll, and vitamin C content in leaf, and shoots carbohydrate content were significantly increased with increasing concentration. The ascorbic acid is more effective at 900 mg/L than other concentrations, whereas putrescine was more effective at 150 mg/L, contrasted with 75 mg/L and control.

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References

Ferguson L, Arapaia M. New subtropical tree crops in California. In: Janick J. and Simon J.(eds). Advances in new crops. Timber Press: Portland; 1990.

Mabberley DJ. A classification for edible Citrus (Rutaceae).Telopea; 1997; 7:167-172. https://doi.org/10.7751/telopea19971007

Baldwin EA. Citrus fruit. In: Seymour GB, Taylor JE, Tucker GA (eds), Biochemistry of Fruit Ripening, Chapman & Hall, London;1993. https://doi.org/10.1007/978-94-011-1584-1_4

Central Bureau of Statistics. Production Report. Annual Statistical Group. Ministry of Planning, Agricultural Statistics Directorate: Publication and Public Relations Department: Baghdad. Iraq; 2020.

Behairy RT. Impact of ascorbic acid on seed germination, seedling growth, and enzyme activity of salt-stressed fenugreek. J Medic Act Plants. 2012;1(3):106-113. https://doi.org/10.7275/R5TT4NW9.

Sharma P, Jha AB, Dubey RS, Pssarak M. Reactive oxygen Species, oxidative damage and antioxidants defense mechanism in plants under stressful conditions. J Botany; 2012; 2012:1-26. https://doi.org/10.1155/2012/217037.

Hasanuzzaman M, Bhuyan MHMB, Zulfiqar F, Raza A, Mohsin SM, Al Mahmud J, Fujita M, Fotopoulos V. Reactive oxygen species and antioxidant defense in plants under abiotic stress: revisiting the crucial role of a universal defense regulator. Antioxidants. 2020; 9(8), 681; https://doi.org/10.3390/antiox9080681.

Hussain SS, Ali M, Ahmad M, Siddique KHM. Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants. Bio-technol Adv. 2011; 29(3):300-311.

http://dx.doi.org/10.1016/j.biotechadv.2011.01.003

Al-Khafaji MA. Plant Growth Regulators. Applications and Utilization in Horticulture. Bookstore for Printing publishing and translating. University of Baghdad: Iraq; 2014.

Mohamed SA, Ahmed HS, El-Bawab AA. Effect of chitosan, Putrescine, and irrigation levels on the drought tolerance of sour orange seedlings. Egypt J Hort. 2018; 45(2):257-273. http://dx.doi.org/10.21608/ejoh.2018.3063.1050.

Wu QS, Zou YN, He XH. Exogenous Putrescine, not Spermine or Spermidine, enhances root mycorrhizal development and plant growth of trifoliate orange (Poncirus trifoliate) seedlings. Int J Agric Biol. 2010;12(4):576-580.

Wu QS, Zou YN, Liuand CY, Lu T. Interacted effect of arbuscular mycorrhizal fungi and polyamines on root system architecture of citrus seedlings. J Integer. Agric. 2012; 11: 1675- 1681. http://dx.doi.org/10.1016/S2095-3119(12)60170-1

Khoshbakht D, Asghari MR, Haghighi M. Influence of foliar application of polyamines on growth, gas-exchange characteristics, and chlorophyll fluorescence in Bakraii citrus under saline conditions. Photosynthet Jal. 2018;56:731-742. http://dx.doi.org/10.1007/s11099-017-0723-

Al-Falahy Th H R. Response of Kumquat (Fortunella margarita) Transplants to Foliar Spray with Bio-stimulator and GA3. IOP Conf. Ser: Earth Environ. Sci. 761 012035 2021; Int Collab Conf Mod Agric Techno. 2020 24-25 March 2021, Iraq https://doi.org/10.1088/1755-1315/761/1/012035

Oleiwi HQ, Al-Falahy THR. Influence of foliar spray with urea and GA3 on some vegetative growth characteristics of mandarin saplings cv. Clementine. Revista. 2023; 8(2) 63. http://dx.doi.org/10.21931/RB/CSS/2023.08.04.08

Alex G. An Introduction to Statistical Methods in GenStat. VSN International, UK; 2011.

Al-Sahhaf FH. Practical Plant Nutrition. Wisdom for Publishing, Translation and Distribution, University of Baghdad, Iraq; 1989.

Tang W, Newton JR. Polyamines promote root elongation and growth by increasing root cell division in regenerated Virginia pine. Plant Cell Rep. 2005;24(10):581-9.

https://doi.org/10.1007/s00299-005-0021-519. Goodwin TW. Chemistry and Biochemistry of plant pigment.v.1 and 2 2nd Academic. Press. London. NewYork. San Francisco;1976. p.1-373.

Sirvastava G Ch, Prasad NK. Modern Methods in Plant Physiology. India; 2010.p.233. https://doi.org/10.59317/9789390083169

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric Method for Determination of sugars and Related Substance. Anal Chem. 1956; 28 (3):350-356. https://doi.org/10.1021/ac60111a017.

Nudrat AA, Shafiq F, Ashraf M. Ascorbic acid - a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Front Plant Sci. 2017;8: 148-159. https://doi.org/10.3389/fpls.2017.00613

Taiz L, Zeiger E. Plant Physiology. 5th Edition, Sinauer Associates Inc., Sunderland, 2010; p.782.

Azad AM, Ibrahim ZR and Abdurrahman AS. Effect of foliar spray of humic acid, ascorbic acid, cultivars and their interactions on growth of olive (Olea europeae L.) transplants cvs. Khithairy and Sorany. IOSR J Agric Vet Sci. 2014.7(4):18-30. http://dx.doi.org/10.9790/2380-07421830

Abd-Alhamid N, Hassan HAS, Aly RBMA, Hagagg LF. Effect of foliar application with putrescine, salicylic, and ascorbic acid on vegetative growth, leaf chemical composition, flowering, and fruit set of picual olive trees. Middle East J Appl Sci. 2019.9(4):996-1012. http://dx.doi.org/10.36632/mejas/2019.9.4.16

Pál M, Szalai G, Gondor OK, Janda T. Unfinished story of polyamines: Role of conjugation, transport and light-related regulation in the polyamine metabolism in plants. Plant Sci. 2021; 308:11-29. https://doi.org/10.1016/j.plantsci.2021.110923

González-Hernández AI, Scalschi L, Vicedo B, Marcos-Barbero EL, Morcuende R, Camañes G. Putrescine: A key metabolite involved in plant development, tolerance and resistance responses to stress. Int J Mol Sci. 2022; 23:2971. https://doi.org/10.3390/ijms23062971.

Bitrián M, Zarza X, Altabella T, Tiburcio AF, Alcázar R. Polyamines under abiotic stress: metabolic crossroads and hormonal crosstalks in plants. Metabolites, 2012;2: 516-5 https://doi.org/10.3390/metabo2030516

Anwar R, Mattoo AK, Handa AK. Polyamine interactions with plant hormones: Crosstalk at several levels. Polyamines, 2015; pp.267-302. Kusano T and Suzuki H (Eds.). Springer Publication, Tokyo, Japan. https://doi.org/10.1007/978-4-431-55212-3_22

Handa AK, Fatima T, Mattoo AK. Polyamines: Bio-molecules with diverse functions in plant and human health and disease. Front Chem. 2018; 6:10

https://doi.org/10.3389%2Ffchem.2018.00010

Stephanie S, De Smet I. Root system architecture: Insights from Arabidopsis and cereal crops. Philos. Trans R Soc Lond B Biol Sci. 2012; 367(1595): 1441-1452.

https://doi.org/10.1098%2Frstb.2011.0234

Al-Falahy THR, Al-Samaraie OHM. Effect of spraying with potassium and licorice root extract on some physical and chemical characteristics of date palm cv. Barhee. Int J Agric Stat Sci. 2021; 17(1):1291-12 DocID: https: //connectjournals.com/03899.

Hussein MJ, Al-Falahy THR. Influence of potassium and GA3 on yield and some fruit quality of date palm cv. Barhee. Int J Agric Stat Sci. 2021; 17:1173-1178. https://connectjournals.com/03899.2021.17.1173

Hardeep S, Khezri M, Bushoven J, Benes S, Hadavi F, Brar G. Carbohydrate partitioning and vegetative growth of Citrus nursery trees influenced by varying photoperiods under led lighting. Jape Soc Hort Science. 2022; 4(6): 18-26. https://doi.org/10.2503/hortj.UTD-379

Moallemi N, Khaleghi E, Jafari Z. The effect of ascorbic acid on vegetative growth of olive plants cv." Baghmalek "under water deficit conditions. J Hort Sci. 2021; 34(4):621-632. https://doi.org/10.22067/jhorts4.v34i4.83327

Morteza M, Sarikhani H, Ahmadinejad M, Dehestani A. Putrescine effect on physiological, morphological, and biochemical traits of carrizo citrange and volkameriana rootstocks under flooding stress. Int J Fruit Sci. 2020; 20(2):164-177. https://doi.org/10.1080/15538362.2019.1605560

Gomez MLP and Lajolo FM. Ascorbic acid metabolism in fruits: activity of enzymes involved in synthesis and degradation during ripening in mango and guava. J Sci Food Agric. 2008; 88:756-762. http://dx.doi.org/10.1002/jsfa.3042

Nicholas S. Ascorbic acid metabolism and functions: Comparison of plants and mammals. Free Rad Bio Med. 2018; 122:116-129. https://doi.org/10.1016/j.freeradbiomed.2018.03.033

Mario F, Amaya I, Valpuesta V, Botella MA. Vitamin C, content in fruits: Biosynthesis and regulation. Front Plant Sci. 2019; 9(6): 1-21. https://doi.org/10. 3389/fpls.2018.02006

Published

08-08-2024

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How to Cite

1.
Taghreed AH, Thamer HRA-F. Impact of exogenous ascorbic acid and putrescine on vegetative, root system morphology and chemical composition of clementine Mandarin saplings. Plant Sci. Today [Internet]. 2024 Aug. 8 [cited 2024 Nov. 23];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/4075

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Research Articles