Research communications
Vol. 5 No. 3 (2018)
Assessment of diurnal variation in Ocimum sanctum Linn. by gas chromatographic fingerprint analysis coupled with chemometric methods
K.B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar – 382023, Gujarat, India
K.B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar – 382023, Gujarat, India
K.B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar – 382023, Gujarat, India
Abstract
Vast intra-specific variations, especially diurnal, geographical and seasonal, have been reported in the chemical composition of essential oils of Ocimum species. The study was conducted to assess diurnal variation in the chemical composition of the leaves of Ocimum sanctum. The leaf samples collected at different times of the day were analyzed by gas chromatography coupled with flame ionization detector (GC-FID). The chromatographic fingerprints of different leaf samples were analyzed by chemometric methods like principal component analysis and hierarchical cluster analysis. No significant difference was found in the chemical compositions of the leaf samples collected at different times of the day. The results lead to a conclusion that O. sanctum does not exhibit diurnal variation in its chemical composition, unlike O. gratissimum.
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