Molecular identification and characterization of pathogenicity of fungal pathogens associated with crown rot disease in banana from Odisha, India

Authors

  • Manasranjan Rout Department of Plant Pathology, Faculty of Agricultural Sciences , Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0009-0005-1262-3656
  • Shyama Sundar Mahapatra Department of Plant Pathology, Faculty of Agricultural Sciences , Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0009-0004-6719-8020
  • Sushree Suparna Mahapatra Department of Plant Pathology, Faculty of Agricultural Sciences , Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0000-0002-6759-5841
  • Gopa Mishra Department of Fruit Sciences, Faculty of Agricultural Sciences, Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0009-0003-7666-2975
  • Sweta Shikta Mahapatra Department of Agriculture and Allied Sciences, Faculty of Agriculture and Allied Sciences, C V Raman Global University, Vidyanagar, Bhubaneswar, Odisha, 752 054, India https://orcid.org/0000-0002-2401-9887
  • Debashish Dash Department of Agriculture and Allied Sciences, Faculty of Agriculture and Allied Sciences, C V Raman Global University, Vidyanagar, Bhubaneswar, Odisha, 752 054, India https://orcid.org/0000-0003-3605-0002
  • Diptanu Datta Department of Plant Pathology, Faculty of Agricultural Sciences , Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0000-0002-6951-9746
  • Tapas Ranjan Das Department of Plant Pathology, Faculty of Agricultural Sciences , Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0000-0003-3749-8415
  • Subrat Kumar Mahapatra Department of Agricultural Statistics, Faculty of Agricultural Sciences (IAS), Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, 751 003, India https://orcid.org/0000-0001-9335-8455

DOI:

https://doi.org/10.14719/pst.4151

Keywords:

banana, crown rot, Fusarium equiseti, molecular identification, pathogenicity test

Abstract

Crown rot disease on banana varieties (Champa, Patakpura and G9) was identified in Odisha's 10 coastal districts during the post-harvest storage stage in 2022-23. Five crown fragments were taken for isolation from each variety of bananas based on symptom developments. Agar plate and standard blotter technique isolation procedures have been used to isolate 1210 fungal colonies, of which 584 representative colonies were purified from banana crown fragments. Different fungal colonies were purified, characterized and identified using morphological and molecular methods from G9 (244), Patakpura (205) and Champa (135) fungal colonies. Morphological identification of Fusarium and Colletotrichum isolates was madeusing colony morphology, pigmentation, the growth rate on the PDA plate and microscopic observation. Molecular identification of Fusarium and Colletotrichum isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA, which includes 5.8S rDNA. Fusarium verticillioides, Fusarium equiseti, Fusarium oxysporum and Colletotrichum gloeosporioides were the 4 fungal isolates identified. The sequences of these fungi were deposited in the GenBank database (NCBI) under the accession numbers OQ363325 (Fusarium verticillioides), OP735534 (Fusarium equiseti), OQ438654 (Fusarium oxysporum) and PP448030 (Colletotrichum gloeosporioides). The neighbor-joining method was used to conduct phylogenetic analysis on the gene sequences. Pathogenicity test results revealed that all 4 isolated fungal strains produced similar crown rot symptoms on 3 varieties of banana fruits. Disease severity index and AUDPC indicated that the Fusarium strain is the most aggressive crown rot pathogen and the G9 is the more susceptible banana variety to crown rot pathogens in Odisha.

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Published

17-01-2025 — Updated on 25-01-2025

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Rout M, Mahapatra SS, Mahapatra SS, Mishra G, Mahapatra SS, Dash D, Datta D, Das TR, Mahapatra SK. Molecular identification and characterization of pathogenicity of fungal pathogens associated with crown rot disease in banana from Odisha, India. Plant Sci. Today [Internet]. 2025 Jan. 25 [cited 2025 Mar. 30];12(1). Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/4151

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