Enhancing plant resilience and drought stress in green gram through seed priming with nodule-associated plant probiotics

Vinoth Kumar Muniyappan; K Sundaralingam; U Sivakumar; Vijaya V Geetha; R Jerlin; N Shobana; V Babu Rajendra  Prasad; T Murugeshwari

doi:10.14719/pst.4603

Authors

Vinoth Kumar Muniyappan Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0003-3869-0003
K Sundaralingam Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0009-0001-5997-6441
U Sivakumar Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India https://orcid.org/0000-0002-7116-1317
Vijaya V Geetha Krishi Vigyan Kendra, Tamil Nadu Agricultural University, Tindivanam 604 002, India https://orcid.org/0000-0002-7964-5631
R Jerlin Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0002-4617-8715
N Shobana Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India https://orcid.org/0000-0003-1177-4899
V Babu Rajendra Prasad Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India https://orcid.org/0000-0002-9743-7721
T Murugeshwari Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0009-0003-1075-5220

DOI:

https://doi.org/10.14719/pst.4603

Keywords:

germination, seed vigour index, dehydrogenase activity, catalase, superoxide dismutase

Abstract

Drought stress is a critical environmental stress that hinders plant growth and development. Most pulses are grown in rainfed ecosystems, significantly affected during drought conditions. To address this predicament in this study, a liquid microbial consortium of nodule-associated plant probiotics was used for seed priming of Greengram to assess its efficacy in alleviating drought stress and improving seed quality attributes. Applying an optimum dose of plant probiotics at the appropriate stage enhances plant productivity, improving tolerance to stresses and reducing dependence on harmful agrochemicals. To optimize the dose, two green gram seeds viz., VBN4 and CO8, were subjected to various concentrations of nodule-associated plant probiotics (NAPP), namely, 2, 4, 6 and 8%, along with the control and hydropriming. In the present study, 2% NAPP improved the physiological and biochemical parameters of the green gram, such as germination, seed vigour index, protein content, dehydrogenase activity and ?-amylase in both varieties. The primed seeds were further evaluated under moisture stress conditions by exposing them to various concentration levels of Polyethylene Glycol 6000 ranging from -2 to -6 bar. The experiment revealed that increasing the concentration of PEG 6000 above in -4 bar reduced germination and seedling vigour in both green gram varieties. However, green gram seeds primed with 2% NAPP showed greater moisture stress resistance than nonprimed seeds. The highest activity of stress-related enzymes such as catalase, peroxidase and super oxidase dismutase triggered by NAPP in green gram plants was observed to have potential for drought stress management. This study highlights the importance of NAPP as a potential seed priming agent for improving seed germination and vigour under moisture stress.

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References

Raman J, Kim JS, Choi KR, Eun H, Yang D, Ko YJ, Kim SJ. Application of lactic acid bacteria (LAB) in sustainable agriculture: advantages and limitations. International Journal of Molecular Sciences. 2022;23(14):7784. https://doi.org/10.3390/ijms23147784.

Mostafavi K, Geive HS, Dadresan M, Zarabi M. Effects of drought stress on germination indices of corn hybrids (Zea mays L.). International Journal of Agri Science. 2011; 1(1): 10-18.

Narayanan GS, Prakash M, Kumar VR. Effect of integrated seed treatments on growth, seed yield and quality parameters in black gram [Vigna mungo (L.) Hepper]. Indian Journal of Agricultural Research. 2017;51(6):556-61. https://doi.org/10.18805/IJARe.A-4106

Hussain S, Zheng M, Khan F, Khaliq A, Fahad S, Peng S, Huang J, Cui K, Nie L. Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Scientific Reports. 2015 Jan 29;5(1):8101. https://doi.org/10.1038/srep08101

Kazimierczak R, Srednicka-Tober D, Hallmann E, Kopczy?ska K, Zarzynska K. The impact of organic vs. conventional agricultural practices on selected quality features of eight potato cultivars. Agronomy. 2019;9(12):799. https://doi.org/10.3390/ agronomy9120799.

Sarkar D, Singh S, Parihar M, Rakshit A. Seed biopriming with microbial inoculants: A tailored approach to improve crop performance, nutritional security and agricultural sustainability for smallholder farmers. Current Research in Environmental Sustainability. 2021; 3: 100093. https://doi.org/10.1016/j.crsust.2021.100093.

Patel S, Sayyed RZ, Saraf M. Bacterial determinants and plant defense induction: their role as biocontrol agents in sustainable agriculture. Plant, Soil and Microbes: Volume 2: Mechanisms and Molecular Interactions. 2016:187-204. https://doi.org/10.1007/978-3-319-29573-2_9.

Narayanasamy S, Thangappan S, Uthandi S. Plant growth-promoting Bacillus sp. cahoots moisture stress alleviation in rice genotypes by triggering antioxidant defense system. Microbiological Research. 2020;239:126518. https://doi.org/10.1016/j.micres.2020.1 26518.

Khattak GS, Ashraf M, Elahi T, Abbas G. Selection for large seed size at the seedling stage in mungbean (Vigna radiata (L.) Wilczek). Breeding Science. 2003;53(2):141-3. https://doi. org/10.1270/jsbbs .53.141

IMD. Annual Climate Summary-2022 National Climate Centre, India Meteorological Department. 2022; p24.

Geetha Thanuja K, Annadurai B, Thankappan S, Uthandi S. Non-rhizobial endophytic (NRE) yeasts assist nodulation of Rhizobium in root nodules of blackgram (Vigna mungo L.). Archives of Microbiology. 2020;202:2739-49. https://doi.org/10.1007/s00203-020-019 83-z.

Raja SR, Uthandi S. Non-rhizobial nodule associated bacteria (NAB) from blackgram (Vigna mungo L.) and their possible role in plant growth promotion. Madras Agricultural Journal. 2019;106 (march (1-3):1. https://doi.org/10.29321 /MAJ.2019.000291.

Annadurai B, Kennedy ZJ, Uthandi S. Drought tolerant Rhizobium sp. VRE1 induced osmotic stress tolerance, seed germination and seedling vigor in blackgram (Vigna mungo L.). International Journal of Ecology and Environmental Sciences. 2020;2(3):37-42.

ISTA. International rules for seed testing. The International Seed Testing Association-ISTA, Bassersdorf, Switzerland. 2019; pp. i-19-8.

AbdulBaki AA, Anderson JD. (Vigor determination in soybean seed by multiple criteria. Crop Sci. 1973;13:630-3.https://doi.org/10.2135/cropsci1973.0011183X60013x.

Ali-Khan ST, Youngs CG. Variation in protein content of field peas. Canadian Journal of Plant Science. 1973;53(1):37-41. https://doi.org/10.4141/cjps73-005.

Presley JT. Relationship of protoplast permeability to cotton seed viability and predisposition to seedling disease. Plant Disease Reporter. 1958; 42(7), 852.

Kittock DL, Law AG. Relationship of seedling vigor to respiration and tetrazolium chloride reduction by germinating wheat seeds 1. Agronomy Journal. 1968 May;60(3):286-8. https:// doi.org/10.2134/agronj 1968.00021962006000030012x.

Paul AK, Mukherji S, Sircar SM. Metabolic changes in rice seeds during storage. Indian Journal of Agricultural Science. 1970; 40 (12):1031-1036.

Michel BE, Kaufmann MR. The osmotic potential of polyethylene glycol 6000. Plant physiology. 1973;51(5):914-6. https://doi.org/10.1104/pp.51.5.914.

Giannopolitis CN, Ries SK. Superoxide dismutases: I. Occurrence in higher plants. Plant physiology. 1977;59(2):309-14. https://doi.org/10.1104/pp.59.2.309.

Aebi H. Catalase in vitro. Meth. Enzymol. 1984, 105, 121–126. https://doi.org/10.1016 /S00 76-6879(84)05016-3.

Malik CP, Singh MP. Peroxidase, Plant Enzymology and Histoenzymology Assay. (1980). Kalyani Publishers. New Delhi. https://doi.org/10.1016/j.colsurfb.2007.01.004.

Oshunsanya SO, Nwosu NJ, Li Y. Abiotic stress in agricultural crops under climatic conditions. Sustainable agriculture, forest and environmental management. 2019:71-100. https://doi.org /10.100 7/978-981-13-6830-1_3.

Kaushal M, Wani SP. Rhizobacterial-plant interactions: strategies ensuring plant growth promotion under drought and salinity stress. Agriculture, Ecosystems and Environment. 2016 Sep 1;231:68-78. https://doi.org/10.1016/j.agee.2016.06.031.

Singh A, Banerjee A, Roychoudhury A. Seed priming with calcium compounds abrogate fluoride-induced oxidative stress by upregulating defence pathways in an indica rice variety. Protoplasma. 2020;257:767-82. https://doi.org/10.1007/s00709-019-01460-5.

Lastochkina O, Garshina D, Ivanov S, Yuldashev R, Khafizova R, Allagulova C, Fedorova K, Avalbaev A, Maslennikova D, Bosacchi M. Seed priming with endophytic Bacillus subtilis modulates physiological responses of two different Triticum aestivum L. cultivars under drought stress. Plants. 2020;9(12):1810. https://doi.org/10.3390/plants9121810

Chin JM, Lim YY, Ting AS. Biopriming vegetable seeds with metal-tolerant biocontrol agents to enhance tolerance to biotic and abiotic stress. Biological Control. 2022;174:105035. https://doi.org/10.1016/j.biocontrol.2022.105035.

Fincher GB. Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu. Rev. Plant Physiol. Plant Mol Biol 1989; 40: 305–345. https://doi.org/10.1146/annurev.pp.40.060189.001513

Gubler F, Kalla R, Roberts JK, Jacobsen JV. Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for Myb transactivation of a high-pI alpha-amylase gene promoter. The Plant Cell. 1995 Nov 1;7(11):1879-91. https://doi.org/10.1105/tpc.7.11.1879

Kaneko M, Itoh H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M. The ?-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiology. 2002;128(4):1264-70. https://doi.org/10.1104/pp.010785

Sharma P, Bhatt A, Jyoti B. Effect of seed bio-priming with microbial inoculants on plant growth, yield and yield contributing characters in soybean [Glycine max (L.) Merril]. International Journal of Economic Plants. 2018;5(May, 2):053-8.

Raja K, Sivasubramaniam K, Anandham R. Seed infusion with liquid microbial consortia for improving germination and vigour in blackgram [Vigna mungo (L.) Hepper]. Legume Research-An International Journal. 2019;42(3):334-40. https://doi.org/10. 18805/LR-3948.

Monisha S.Studies on harnessing the potential of bioinoculants for the management of abiotic and biotic stress in black gram [Vigna mungo (L.) Hepper] Ph.D. (Ag) Thesis, Tamil Nadu Agricultural University, Coimbatore;2023.

Benmrid B, Ghoulam C, Zeroual Y, Kouisni L, Bargaz A. Bioinoculants as a means of increasing crop tolerance to drought and phosphorus deficiency in legume-cereal intercropping systems. Communications Biology. 2023;6(1):1016. https://doi.org/10.1038/s42003-023-05399-5

Spaepen S, Vanderleyden J. Auxin and plant-microbe interactions. Cold Spring Harbor perspectives in biology. 2011;3(4):a001438. https://doi.org/10.1101/cshperspect. a001438

Muniyappan VK, Tamilmani E, Desikan R, Ranganathan U. Influence of groundnut seed viability on biodiesel feedstock quality. Industrial Crops and Products. 2019;140:111697. https://doi.org/10.1016/j.indcrop.2019.111697.

Revathi R, Vanangamudi K. Biopriming of maize hybrid seeds with biocontrol agents to improve germination and vigour. Madras Agricultural Journal. 2014;101(1/3), 59-65.

Chaudhary S, Priya M, Jha UC, Pratap A, HanumanthaRao B, Singh I, Prasad PV, Siddique KH, Nayyar H. Approaches toward developing heat and drought tolerance in mungbean. Developing climate resilient grain and forage legumes. 2022:205-34.

Patten CL, Glick BR. Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and environmental microbiology. 2002;68(8):3795-801. https://doi.org/10.1128/AEM.68.8.3795-3801.2002.

Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The chemistry of reactive oxygen species (ROS) revisited: outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. International Journal of Molecular Sciences. 2021;22(9):4642. https://doi.org/10.3390/ijms22094642.

Piri R, Moradi A, Balouchi H, Salehi A. Improvement of cumin (Cuminum cyminum) seed performance under drought stress by seed coating and biopriming. Scientia Horticulturae. 2019 Nov 17;257:108667. https://doi.org/10.1016/j.scienta.2019.108667.

Abdelaal K, AlKahtani M, Attia K, Hafez Y, Király L, Künstler A. The role of plant growth-promoting bacteria in alleviating the adverse effects of drought on plants. Biology. 2021 Jun 11;10(6):520.https://doi.org/10.3390/biology10060520