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Plant Science Today (PST)

Review Articles

Vol. 11 No. 4 (2024)

Unraveling the intriguing potential of protein-rich microbial biostimulants for horticultural crops

DOI
https://doi.org/10.14719/pst.4784
Submitted
23 August 2024
Published
18-10-2024 — Updated on 23-10-2024
Versions

Abstract

Nutritional security and minimizing the impact of farming practices on the environment are major challenges in modern farming systems. Currently, the horticulture sector is growing fast and moving towards sustainability and profitability. Indiscriminate and improper use of chemical inputs to ensure high yields of horticultural products could lead to significant contamination of soil and water bodies. Under these circumstances, farmers must optimize their input management to reduce pollution and preserve the economic margin by following sustainable production practices. The use of precision horticulture techniques is more sustainable than conventional to intensive farming methods. Among the various eco-friendly inputs, plant biostimulants are highly effective and can enhance plant growth and production as well as mitigate the adverse effects of abiotic stressors. Protein Hydrolysates (PHs) are a significant class of plant biostimulants based on amino acid and peptide mixtures. Because of their beneficial effects on crop performance, PHs has drawn increased amounts of attention recently. Compared with other biostimulants microbial biostimulants are more prevalent in crop production. A new approach is the formulation of a mixture of plant growth-promoting microorganisms/microbe-derived metabolites and protein hydrolysates as single biostimulants, to nourish the soil, plants and microbes. This review presents a thorough summary of recent research on the postulated modes of action of PHs and microbial biostimulants in horticultural crops. Furthermore, this study highlights the potential of protein hydrolysates and microbial biostimulants and the potential of the protein-rich microbial biostimulants to make horticulture more profitable and to safeguard the environment.

References

  1. Ali SS, Al-Tohamy R, Koutra E, Moawad MS, Kornaros M, Mustafa AM, et al. Nanobiotechnological advancements in agriculture and food industry: Applications, nanotoxicity and future perspectives. Science of the Total Environment. 2021 Oct 20;792:148359. https://doi.org/10.1016/j.scitotenv.2021.148359
  2. Tudi M, Daniel Ruan H, Wang L, Lyu J, Sadler R, Connell D, et al. Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health. 2021 Feb;18(3):1112. https://doi.org/10.3390/ijerph18031112
  3. Grammenou A, Petropoulos SA, Thalassinos G, Rinklebe J, Shaheen SM, Antoniadis V. Biostimulants in the soil–plant interface: ago-environmental implications—a review. Earth Systems and Environment. 2023 Sep;7(3):583-600. https://doi.org/10.1007/s41748-023-00349-x
  4. Colla G, Rouphael Y, Canaguier R, Svecova E, Cardarelli M. Biostimulant action of a plant-derived protein hydrolysate produced through enzymatic hydrolysis. Frontiers in Plant Science. 2014 Sep 9;5:448. https://doi.org/10.3389/fpls.2014.00448
  5. Moreno-Hernández JM, Benítez-García I, Mazorra-Manzano MA, Ramírez-Suárez JC, Sánchez E. Strategies for production, characterization and application of protein-based biostimulants in agriculture: A review. Chilean Journal of Agricultural Research. 2020 Jun;80(2):274-89. http://dx.doi.org/10.4067/S0718-58392020000200274
  6. Kaur M, Bhari R, Singh RS. Chicken feather waste-derived protein hydrolysate as a potential biostimulant for cultivation of mung beans. Biologia. 2021 Jun;76:1807-15. https://doi.org/10.1007/s11756-021-00724-x
  7. Kaushal P, Ali N, Saini S, Pati PK, Pati AM. Physiological and molecular insight of microbial biostimulants for sustainable agriculture. Frontiers in Plant Science. 2023 Jan 30;14:1041413. https://doi.org/10.3389/fpls.2023.1041413
  8. Rouphael Y, Cardarelli M, Bonini P, Colla G. Synergistic action of a microbial-based biostimulant and a plant derived-protein hydrolysate enhances lettuce tolerance to alkalinity and salinity. Frontiers in Plant Science. 2017 Feb 7;8:131. https://doi.org/10.3389/fpls.2017.00131
  9. Di Miceli G, Vultaggio L, Sabatino L, De Pasquale C, La Bella S, Consentino BB. Synergistic effect of a plant-derived protein hydrolysate and arbuscular mycorrhizal fungi on eggplant grown in open fields: A two-year study. Horticulturae. 2023 May 17;9(5):592. https://doi.org/10.3390/horticulturae9050592
  10. Zhang X, Schmidt RE. Hormone containing products' impact on antioxidant status of tall fescue and creeping bentgrass subjected to drought. Crop Science. 2000 Sep;40(5):1344-49. https://doi.org/10.2135/cropsci2000.4051344x
  11. Du Jardin P. The science of plants biostimulants: a bibliographic analysis. EU; 2012.
  12. Coalition B. What are biostimulants?; 2013.
  13. Halpern M, Bar-Tal A, Ofek M, Minz D, Muller T, Yermiyahu U. The use of biostimulants for enhancing nutrient uptake. Advances in Agronomy. 2015 Jan 1;130:141-74. https://doi.org/10.1016/bs.agron.2014.10.001
  14. Colla G, Nardi S, Cardarelli M, Ertani A, Lucini L, Canaguier R, Rouphael Y. Protein hydrolysates as biostimulants in horticulture. Scientia Horticulturae. 2015 Nov 30;196:28-38. https://doi.org/10.1016/j.scienta.2015.08.037
  15. Schaafsma G. Safety of protein hydrolysates, fractions thereof and bioactive peptides in human nutrition. European Journal of Clinical Nutrition. 2009 Oct;63(10):1161-68. https://doi.org/10.1007/s11104-014-2131-8
  16. Baltazar M, Correia S, Guinan KJ, Sujeeth N, Bragança R, Gonçalves B. Recent advances in the molecular effects of biostimulants in plants: An overview. Biomolecules. 2021 Jul 25;11(8):1096. https://doi.org/10.3390/biom11081096
  17. Pasupuleti VK, Braun S. State of the art manufacturing of protein hydrolysates. Protein Hydrolysates in Biotechnology. 2010;11-32. https://doi.org/10.1007/978-1-4020-6674-0_2
  18. Cavani L, Ciavatta C, Gessa C. Determination of free L-and D-alanine in hydrolysed protein fertilisers by capillary electrophoresis. Journal of Chromatography A. 2003 Jan 24;985(1-2):463-69. https://doi.org/10.1016/S0021-9673(02)01733-8
  19. Islam M, Huang Y, Islam S, Fan B, Tong L, Wang F. Influence of the degree of hydrolysis on functional properties and antioxidant activity of enzymatic soybean protein hydrolysates. Molecules. 2022 Sep 19;27(18):6110. https://doi.org/10.3390/molecules27186110
  20. Nardi S, Pizzeghello D, Schiavon M, Ertani A. Plant biostimulants: physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism. Scientia Agricola. 2016;73(1):18-23. https://doi.org/10.1590/0103-9016-2015-0006
  21. Zamora-Sillero J, Gharsallaoui A, Prentice C. Peptides from fish by-product protein hydrolysates and its functional properties: An overview. Marine Biotechnology. 2018 Apr;20:118-30. https://doi.org/10.1007/s10126-018-9799-3
  22. Chalamaiah M, Hemalatha R, Jyothirmayi T. Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chemistry. 2012 Dec 15;135(4):3020-38. https://doi.org/10.1016/j.foodchem.2012.06.100
  23. Gharibzahedi SM, Smith B. Effects of high hydrostatic pressure on the quality and functionality of protein isolates, concentrates and hydrolysates derived from pulse legumes: A review. Trends in Food Science and Technology. 2021 Jan 1;107:466-79. https://doi.org/10.1016/j.tifs.2020.11.016
  24. Adewole TS, Bieni MC, Ogundepo GE, Odekanyin OO, Kuku A. Investigation of functional, antioxidant, anti-inflammatory and antidiabetic properties of legume seed protein hydrolysates. Food Hydrocolloids for Health. 2024 Jun 1;5:100175. https://doi.org/10.1016/j.fhfh.2023.100175
  25. Ertani A, Schiavon M, Muscolo A, Nardi S. Alfalfa plant-derived biostimulant stimulate short-term growth of salt stressed Zea mays L. plants. Plant and Soil. 2013 Mar;364:145-58. https://doi.org/10.1007/s11104-012-1335-z
  26. Sharma S, Pradhan R, Manickavasagan A, Thimmanagari M, Saha D, Singh SS, Dutta A. Production of antioxidative protein hydrolysates from corn distillers solubles: Process optimization, antioxidant activity evaluation and peptide analysis. Industrial Crops and Products. 2022 Sep 15;184:115107. https://doi.org/10.1016/j.indcrop.2022.115107
  27. Buitrón L, Sisa A, Arévalo R, Peñaherrera E, Mosquera M. Toxicological evaluation of peptide hydrolysates from bovine blood meal with antioxidant and antifungal activities. Food and Humanity. 2024 May 1;2:100210. https://doi.org/10.1016/j.foohum.2023.100210
  28. García-Santiago JC, Lozano Cavazos CJ, González-Fuentes JA, Zermeño-González A, Rascon Alvarado E, Rojas Duarte A, et al. Effects of fish-derived protein hydrolysate, animal-based organic fertilisers and irrigation method on the growth and quality of grape tomatoes. Biological Agriculture and Horticulture. 2021 Apr 3;37(2):107-24. https://doi.org/10.1080/01448765.2021.1891458
  29. Prajapati S, Koirala S, Anal AK. Bioutilization of chicken feather waste by newly isolated keratinolytic bacteria and conversion into protein hydrolysates with improved functionalities. Applied Biochemistry and Biotechnology. 2021 Aug;193:2497-515. https://doi.org/10.1007/s12010-021-03554-4
  30. Manninen AH. Protein hydrolysates in sports and exercise: a brief review. Journal of Sports Science and Medicine. 2004 Jun;3(2):60.
  31. Mandal S, Anand U, López-Bucio J, Kumar M, Lal MK, Tiwari RK, et al. Biostimulants and environmental stress mitigation in crops: A novel and emerging approach for agricultural sustainability under climate change. Environ Res. 2023 Sep 15;233:116357. DOI: 10.1016/j.envres.2023.116357
  32. Barrada A, Delisle-Houde M, Nguyen TT, Tweddell RJ, Dorais M. Drench application of soy protein hydrolysates increases tomato plant fitness, fruit yield and resistance to a hemibiotrophic pathogen. Agronomy. 2022 Jul 27;12(8):1761. https://doi.org/10.3390/agronomy12081761
  33. Domingo G, Marsoni M, Álvarez-Viñas M, Torres MD, Domínguez H, Vannini C. The role of protein-rich extracts from Chondrus crispus as biostimulant and in enhancing tolerance to drought stress in tomato plants. Plants. 2023 Feb 13;12(4):845. https://doi.org/10.3390/plants12040845
  34. Ertani A, Pizzeghello D, Francioso O, Sambo P, Sanchez-Cortes S, Nardi S. Capsicum chinensis L. growth and nutraceutical properties are enhanced by biostimulants in a long-term period: Chemical and metabolomic approaches. Frontiers in Plant Science. 2014 Aug 1;5:37. https://doi.org/10.3389/fpls.2014.00375
  35. Brown MA, Stevenson EJ, Howatson G. Whey protein hydrolysate supplementation accelerates recovery from exercise-induced muscle damage in females. Applied Physiology, Nutrition and Metabolism. 2018;43(4):324-30. https://doi.org/10.1139/apnm-2017-0412
  36. Elwaziri E, Ismail H, Abou El-Khair ES, Al-Qahtani SM, Al-Harbi NA, Abd El-Gawad HG, et al. Biostimulant application of whey protein hydrolysates and potassium fertilization enhances the productivity and tuber quality of sweet potato. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2023 Jun 7;51(2):13122. https://doi.org/10.15835/nbha51213122
  37. Mackie IM. General review of fish protein hydrolysates. Animal Feed Science and Technology. 1982 Mar 1;7(2):113-24. https://doi.org/10.1016/0377-8401(82)90045-1
  38. Cerdán M, Sánchez-Sánchez A, Jordá JD, Juárez M, Sánchez-Andreu J. Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency. Journal of Plant Nutrition and Soil Science. 2013 Dec;176(6):859-66. https://doi.org/10.1002/jpln.201200525
  39. Cioroianu T, Sirbu C, Mihalache D, St?nescu AM. Use of protein hydrolysates in organic agriculture. Annals of the University of Craiova-Agriculture, Montanology, Cadastre Series. 2021 Dec 29;51(1):207-16.
  40. Corte L, Dell'Abate MT, Magini A, Migliore M, Felici B, Roscini L, et al. Assessment of safety and efficiency of nitrogen organic fertilizers from animal based protein hydrolysates—a laboratory multidisciplinary approach. Journal of the Science of Food and Agriculture. 2014 Jan 30;94(2):235-45. https://doi.org/10.1002/jsfa.6239
  41. Bulgari R, Franzoni G, Ferrante A. Biostimulants application in horticultural crops under abiotic stress conditions. Agronomy. 2019 Jun 12;9(6):306. https://doi.org/10.3390/agronomy9060306
  42. Wise K, Selby-Pham J, Chai X, Simovich T, Gupta S, Gill H. Fertiliser supplementation with a biostimulant complex of fish hydrolysate, Aloe vera extract and kelp alters cannabis root architecture to enhance nutrient uptake. Scientia Horticulturae. 2024 Jan 1;323:112483. https://doi.org/10.1016/j.scienta.2023.112483
  43. Leporino M, Rouphael Y, Bonini P, Colla G, Cardarelli M. Protein hydrolysates enhance recovery from drought stress in tomato plants: phenomic and metabolomic insights. Frontiers in Plant Science. 2024 Mar 12;15:1357316. https://doi.org/10.3389/fpls.2024.1357316
  44. Gurav R, Nalavade V, Aware C, Vyavahare G, Bhatia SK, Yang YH, et al. Microbial degradation of poultry feather biomass in a constructed bioreactor and application of hydrolysate as bioenhancer to vegetable crops. Environmental Science and Pollution Research. 2020 Jan;27:2027-35. https://doi.org/10.1007/s11356-019-06536-6
  45. Marfà O, Cáceres R, Polo J, Ródenas J. Animal protein hydrolysate as a biostimulant for transplanted strawberry plants subjected to cold stress. In: VI International Strawberry Symposium; 842 2008 Mar 3:pp. 315-18.
  46. https://doi.org/10.17660/ActaHortic.2009.842.57
  47. Ertani A, Schiavon M, Nardi S. Transcriptome-wide identification of differentially expressed genes in Solanum lycopersicon L. in response to an alfalfa-protein hydrolysate using microarrays. Frontiers in Plant Science. 2017 Jul 5;8:1159. https://doi.org/10.3389/fpls.2017.01159
  48. Kumar S, Chinnannan K, Thamilarasan SK, Seralathan M, Shanmuganathan R, Padikasan IA. Enzymatically hydrolysed sago bagasse improves physiological, biochemical and molecular attributes of Solanum lycopersicum. Biocatalysis and Agricultural Biotechnology. 2019 Jan 1;17:499-506. https://doi.org/10.1016/j.bcab.2019.01.005
  49. Bonasia A, Conversa G, Lazzizera C, Elia A. Foliar application of protein hydrolysates on baby-leaf spinach grown at different n levels. Agronomy. 2021 Dec 24;12(1):36. https://doi.org/10.3390/agronomy12010036
  50. Casadesús A, Pérez-Llorca M, Munné-Bosch S, Polo J. An enzymatically hydrolyzed animal protein-based biostimulant (Pepton) increases salicylic acid and promotes growth of tomato roots under temperature and nutrient stress. Frontiers in Plant Science. 2020 Jul 1;11:953. https://doi.org/10.3389/fpls.2020.00953
  51. Aktsoglou DC, Kasampalis DS, Sarrou E, Tsouvaltzis P, Chatzopoulou P, Martens S, Siomos AS. Protein hydrolysates supplement in the nutrient solution of soilless grown fresh peppermint and spearmint as a tool for improving product quality. Agronomy. 2021 Feb 11;11(2):317. https://doi.org/10.3390/agronomy11020317
  52. El-Nakhel C, Ciriello M, Formisano L, Pannico A, Giordano M, Gentile BR, et al. Protein hydrolysate combined with hydroponics divergently modifies growth and shuffles pigments and free amino acids of carrot and dill microgreens. Horticulturae. 2021 Sep 3;7(9):279. https://doi.org/10.3390/horticulturae7090279
  53. Su J, Jiang J, Zhang F, Liu Y, Ding L, Chen S, Chen F. Current achievements and future prospects in the genetic breeding of chrysanthemum: a review. Horticulture Research. 2019 Dec 1;6. https://doi.org/10.1038/s41438-019-0193-8
  54. Carillo P, Pannico A, Cirillo C, Ciriello M, Colla G, Cardarelli M, et al. Protein hydrolysates from animal or vegetal sources affect morpho-physiological traits, ornamental quality, mineral composition and shelf-life of Chrysanthemum in a distinctive manner. Plants. 2022 Sep 5;11(17):2321. https://doi.org/10.3390/plants11172321
  55. Peron G, Franceschi C, Da Dalt C, Ferrarese I, Sut S, Dall’Acqua S. Biostimulation of Calendula officinalis with a soy protein hydrolysate induces flower and plant biomass and flower count by reversibly altering the floral metabolome. Industrial Crops and Products. 2024 Aug 1;214:118508. https://doi.org/10.1016/j.indcrop.2024.118508.
  56. Cristiano G, De Lucia B. Petunia performance under application of animal-based protein hydrolysates: effects on visual quality, biomass, nutrient content, root morphology and gas exchange. Frontiers in Plant Science. 2021 Jun 14;12:640608. https://doi.org/10.3389/fpls.2021.640608
  57. Plaza BM, Gómez-Serrano C, Acién-Fernández FG, Jimenez-Becker S. Effect of microalgae hydrolysate foliar application (Arthrospira platensis and Scenedesmus sp.) on Petunia x hybrida growth. Journal of Applied Phycology. 2018 Aug;30:2359-65. https://doi.org/10.1007/s10811-018-1427-0
  58. Tütüncü M. Effects of protein hydrolysate derived from anchovy by-product on plant growth of primrose and root system architecture analysis with machine learning. Horticulturae. 2024 Apr 15;10(4):400. https://doi.org/10.3390/horticulturae10040400
  59. Turner BL, Blackwell MS. Isolating the influence of pH on the amounts and forms of soil organic phosphorus. European Journal of Soil Science. 2013 Apr;64(2):249-59. https://doi.org/10.1111/ejss.12026
  60. Jacoby R, Peukert M, Succurro A, Koprivova A, Kopriva S. The role of soil microorganisms in plant mineral nutrition, current knowledge and future directions. Frontiers in Plant Science. 2017 Sep 19;8:1617. https://doi.org/10.3389/fpls.2017.01617
  61. Tejada M, Benítez C, Gómez I, Parrado J. Use of biostimulants on soil restoration: Effects on soil biochemical properties and microbial community. Applied Soil Ecology. 2011 Sep 1;49:11-17. https://doi.org/10.1016/j.apsoil.2011.07.009
  62. Luziatelli F, Ficca AG, Colla G, Baldassarre Švecová E, Ruzzi M. Foliar application of vegetal-derived bioactive compounds stimulates the growth of beneficial bacteria and enhances microbiome biodiversity in lettuce. Frontiers in Plant Science. 2019 Feb 5;10:60. https://doi.org/10.3389/fpls.2019.00060
  63. Backer R, Rokem JS, Ilangumaran G, Lamont J, Praslickova D, Ricci E, et al. Plant growth-promoting rhizobacteria: context, mechanisms of action and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science. 2018 Oct 23;9:1473. https://doi.org/10.3389/fpls.2018.01473
  64. Romano I, Ventorino V, Pepe O. Effectiveness of plant beneficial microbes: overview of the methodological approaches for the assessment of root colonization and persistence. Frontiers in Plant Science. 2020 Jan 31;11:6. https://doi.org/10.3389/fpls.2020.00006
  65. Rouphael Y, Colla G. Synergistic biostimulatory action: Designing the next generation of plant biostimulants for sustainable agriculture. Frontiers in Plant Science. 2018 Nov 13;9:1655. https://doi.org/10.3389/fpls.2018.01655
  66. Castiglione AM, Mannino G, Contartese V, Bertea CM, Ertani A. Microbial biostimulants as response to modern agriculture needs: Composition, role and application of these innovative products. Plants. 2021 Jul 27;10(8):1533. https://doi.org/10.3390/plants10081533
  67. Begum N, Qin C, Ahanger MA, Raza S, Khan MI, Ashraf M, et al. Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Frontiers in Plant Science. 2019 Sep 19;10:1068. https://doi.org/10.3389/fpls.2019.01068
  68. Lu Q, Jin L, Wang P, Liu F, Huang B, Wen M, Wu S. Effects of interaction of protein hydrolysate and arbuscular mycorrhizal fungi effects on Citrus growth and expressions of stress-responsive genes (Aquaporins and SOSs) under salt stress. Journal of Fungi. 2023 Sep 29;9(10):983. https://doi.org/10.3390/jof9100983
  69. Benito P, Celdrán M, Bellón J, Arbona V, González-Guzmán M, Porcel R, et al. The combination of a microbial and a non-microbial biostimulant increases yield in lettuce (Lactuca sativa) under salt stress conditions by up-regulating cytokinin biosynthesis. Journal of Integrative Plant Biology. 2024. https://doi.org/10.1111/jipb.13755
  70. Munaro D, Mazo CH, Bauer CM, da Silva Gomes L, Teodoro EB, Mazzarino L, et al. A novel biostimulant from chitosan nanoparticles and microalgae-based protein hydrolysate: Improving crop performance in tomato. Scientia Horticulturae. 2024 Jan 1;323:112491. https://doi.org/10.1016/j.scienta.2023.112491
  71. Li J, Brecht JK, Kim J, Bailey LS, Kamat MN, Basso KB, et al. Seaweed extract and microbial biostimulants show synergistic effects on improving organic strawberry production. HortScience. 2024 Aug 1;59(8):1114-26. https://doi.org/10.21273/HORTSCI17647-23
  72. Gürsoy M. Alone or combined effect of seaweed and humic acid applications on eapeseed (Brassica napus L.) under salinity stress. Journal of Soil Science and Plant Nutrition. 2024 Apr 19;1-3. https://doi.org/10.1007/s42729-024-01759-0
  73. Raguraj S, Kasim S, Jaafar NM, Nazli MH. Influence of chicken feather waste derived protein hydrolysate on the growth of tea plants under different application methods and fertilizer rates. Environmental Science and Pollution Research. 2023 Mar;30(13):37017-28. https://doi.org/10.1007/s11356-022-24758-z
  74. Szopa D, Skrzypczak D, Izydorczyk G, Chojnacka K, Korczy?ski M, Witek-Krowiak A. Evaluation of tenebrio molitor protein hydrolysates as biostimulants improving plants growth and root architecture. Journal of Cleaner Production. 2023 May 15;401:136812. https://doi.org/10.1016/j.jclepro.2023.136812
  75. Zamljen T, Medic A, Veberic R, Hudina M, Grohar MC, Slatnar A. Influence of hydrolyzed animal protein-based biostimulant on primary, soluble and volatile secondary metabolism of Genovese and Greek-type basil grown under salt stress. Scientia Horticulturae. 2023 Sep 1;319:112178. https://doi.org/10.1016/j.scienta.2023.112178
  76. Ciriello M, Campana E, De Pascale S, Rouphael Y. Implications of vegetal protein hydrolysates for improving nitrogen use efficiency in leafy vegetables. Horticulturae. 2024 Jan 30;10(2):132. https://doi.org/10.3390/horticulturae10020132
  77. Costa OY, Chang J, Li J, van Lith W, Kuramae EE. Unraveling the impact of protein hydrolysates on rhizosphere microbial communities: Source matters. Applied Soil Ecology. 2024 Apr 1;196:105307. https://doi.org/10.1016/j.apsoil.2024.105307
  78. Cristofano F, El-Nakhel C, Pannico A, Giordano M, Colla G, Rouphael Y. Foliar and root applications of vegetal-derived protein hydrolysates differentially enhance the yield and qualitative attributes of two lettuce cultivars grown in floating system. Agronomy. 2021 Jun 11;11(6):1194. https://doi.org/10.3390/agronomy11061194
  79. Lei H, Zhang J, Jia C, Feng J, Liang L, Cheng Q, et al. Foliar application of fish protein peptide improved the quality of deep-netted melon. Journal of Plant Nutrition. 2023 Sep 14;46(15):3683-96. https://doi.org/10.1080/01904167.2023.2210607
  80. Meggio F, Trevisan S, Manoli A, Ruperti B, Quaggiotti S. Systematic investigation of the effects of a novel protein hydrolysate on the growth, physiological parameters, fruit development and yield of grapevine (Vitis vinifera L., cv Sauvignon Blanc) under water stress conditions. Agronomy. 2020 Nov 14;10(11):1785. https://doi.org/10.3390/agronomy10111785
  81. Agliassa C, Mannino G, Molino D, Cavalletto S, Contartese V, Bertea CM, Secchi F. A new protein hydrolysate-based biostimulant applied by fertigation promotes relief from drought stress in Capsicum annuum L. Plant Physiology and Biochemistry. 2021 Sep 1;166:1076-86. https://doi.org/10.1016/j.plaphy.2021.07.015
  82. Kalymbetov GY, Kedelbayev BS, Yelemanova ZR, Sapargaliyeva B. Effects of different biostimulants on seed germination of sorghum plants. Journal of Ecological Engineering. 2023;24(3). https://doi.org/10.12911/22998993/157568
  83. Alarcón-Zayas A, Hernández-Montiel LG, Medina-Hernández D, Rueda-Puente EO, Ceiro-Catasú WG, Holguín-Peña RJ. Effects of Glomus fasciculatum, Azotobacter chroococcum and vermicompost leachate on the production and quality of tomato fruit. Microbiology Research. 2024 Jan 11;15(1):187-95. https://doi.org/10.3390/microbiolres15010013
  84. Vendruscolo EP, Sant’Ana GR, Lima SF, Gaete FI, Bortolheiro FP, Serafim GM. Biostimulant potential of Azospirillum brasilense and nicotinamide for hydroponic pumpkin cultivation. Revista Brasileira de Engenharia Agrícola e Ambiental. 2024 Mar 4;28(4):e278962. https://doi.org/10.1590/1807-1929/agriambi.v28n4e278962
  85. Bhagawat Prasad DV, Khokhar D, Guhey A. Impact of bio-fertilizers and bio-stimulant on vegetative growth parameters of African marigold (Tagetes erecta L.).
  86. Younes NA, Anik TR, Rahman MM, Wardany AA, Dawood MF, Tran LS, et al. Effects of microbial biostimulants (Trichoderma album and Bacillus megaterium) on growth, quality attributes and yield of onion under field conditions. Heliyon. 2023 Mar 1;9(3). https://doi.org/10.1016/j.heliyon.2023.e14203
  87. Poudel P, Whittinghill L, Kobayashi H, Lucas S. Evaluating the effects of Bacillus subtilis treatment and planting depth on saffron (Crocus sativus L.) production in a green roof system. HortScience. 2023 Oct 1;58(10):1267-74. https://doi.org/10.21273/HORTSCI17220-23
  88. Yi Y, Hou Z, Shi Y, Zhang C, Zhu L, Sun X, et al. Pseudomonas fluorescens RB5 as a biocontrol strain for controlling wheat sheath blight caused by Rhizoctonia Cerealis. Agronomy. 2023 Jul 27;13(8):1986. https://doi.org/10.3390/agronomy13081986
  89. Pastor NA, Cejas LG, Guiñazú LB, Rovera M, Torres AM. Inoculation of tomato roots with single and mixed suspensions of Trichoderma harzianum ITEM 3636 conidia and Pseudomonas putida PCI2 cells. International Journal of Vegetable Science. 2024 Jan 2;30(1):56-73. https://doi.org/10.1080/19315260.2023.2298007
  90. Mowafy AM, Khalifa S, Elsayed A. Brevibacillus DesertYSK and Rhizobium MAP7 stimulate the growth and pigmentation of Lactuca sativa L. Journal of Genetic Engineering and Biotechnology. 2023 Dec 1;21(1):17. https://doi.org/10.1186/s43141-023-00465-1

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