Exploring the anticancer and apoptotic potential of Verbena officinalis L. extracts in combination with conventional chemotherapy for pancreatic cancer: A multifaceted in vitro study on PANC-1 cells
DOI:
https://doi.org/10.14719/pst.4793Keywords:
anticancer activity, apoptosis, pancreatic cancer, Verbena officinalisAbstract
This study evaluated the anticancer and apoptotic potential of different Verbena officinalis (VO) extracts on human pancreatic adenocarcinoma PANC-1 cells compared to normal HUVEC cells. We assessed the antioxidant capacity through total phenolic content (TPC) as milligrams of gallic acid equivalents (GAE) per gram and DPPH radical scavenging assays. Our results showed that the aqueous VO extract exhibited a high TPC of 875.70 ± 0.75 mg GAE/g, while the methanol extract demonstrated the most effective DPPH scavenging with an IC50 60.65 ?g/mL. Cytotoxicity assays, both with VO alone and in combination with gemcitabine, revealed potent anticancer effects against PANC-1 cells (IC25-IC50 value: 25-37.40 ?g/mL) but no significant impact on normal HUVECs. Apoptosis analysis using flow cytometry indicated that VO with gemcitabine induced more significant apoptosis than either treatment alone. Furthermore, VO extracts modulated the expression of apoptotic genes, reducing anti-apoptotic Bcl-2 levels while increasing pro-apoptotic Bax and Caspase-3 in PANC-1 cells, as confirmed by qRT-PCR. This study supports the potential of VO as an anticancer agent, offering insights into the mechanisms underlying its traditional medicinal applications. Further research could establish VO as an effective complementary natural therapy for pancreatic cancer.
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Jee W, Ko HM, Park DI, Park YR, Park SM, Kim H, et al. Momordicae Semen inhibits migration and induces apoptotic cell death by regulating c-Myc and CNOT2 in human pancreatic cancer cells. Sci Rep. 2023;13(1):12800.https://doi.org/10.1038/s41598-023-39840-w
Mesas C, Quiñonero F, Doello K, Revueltas JL, Perazzoli G, Cabeza L, et al. Active Biomolecules from Vegetable Extracts with Antitumoral Activity against Pancreas Cancer: A Systematic Review (2011-2021). Life. 2022;12(11):1765. https://doi.org/10.3390/life12111765
Chebaro Z, Abdallah R, Badran A, Hamade K, Hijazi A, Maresca M, et al. Study of the antioxidant and anti-pancreatic cancer activities of Anchusa strigosa aqueous extracts obtained by maceration and ultrasonic extraction techniques. Front Pharmacol. 2023;14:1201969. https://doi.org/10.3389/fphar.2023.1201969
Agrawal S, Das R, Singh AK, Kumar P, Shukla PK, Bhattacharya I, et al. Network pharmacology-based anti-pancreatic cancer potential of kaempferol and catechin of Trema orientalis L. through computational approach. Med Oncol. 2023;40(5):133. https://doi.org/10.1007/s12032-023-01996-w
Dairo G, Ilesanmi A, Balogun T, Ward M, Soendergaard M, Determan J. Computational evaluation of bioactive compounds from Viscum album (mistletoe) as inhibitors of p63 for pancreatic cancer treatment. J Biomol Struct Dyn. 2023;41(24):15610-24. https://doi.org/10.1080/07391102.2023.2191133
Ansari D, Tingstedt B, Andersson B, Holmquist F, Sturesson C, Williamsson C, et al. Pancreatic cancer: yesterday, today and tomorrow. Future Oncol. 2016;12(16):1929-46. https://doi.org/10.2217/fon-2016-0010
Yao H, Qiao YJ, Zhao YL, Tao XF, Xu LN, Yin LH, et al. Herbal medicines and nonalcoholic fatty liver disease. World J Gastroenterol. 2016;22(30):6890-905.https://doi.org/10.3748/wjg.v22.i30.6890
Modarresi Chahardehi A, Arsad H, Ismail NZ, Lim V. Low cytotoxicity, and antiproliferative activity on cancer cells, of the plant Senna alata (Fabaceae). Revista de Biología Tropical. 2020;69(1):317-30. https://doi.org/10.15517/rbt.v69i1.42144
Rehecho S, Hidalgo O, García-Iñiguez de Cirano M, Navarro I, Astiasarán I, Ansorena D, et al. Chemical composition, mineral content and antioxidant activity of Verbena officinalis L. LWT - Food Science and Technology. 2011;44(4):875-82. https://doi.org/10.1016/j.lwt.2010.11.035
Nisar R, Adhikary S, Ahmad S, Alam MA. In Vitro Antimelanoma Properties of Verbena officinalis Fractions. Molecules. 2022;27(19). https://doi.org/10.3390/molecules27196329
Dai X, Zhou X, Shao R, Zhao R, Yanamandra AK, Xing Z, et al. Bioactive Constituents of Verbena officinalis Alleviate Inflammation and Enhance Killing Efficiency of Natural Killer Cells. Int J Mol Sci. 2023;24(8). https://doi.org/10.3390/ijms24087144
Bilia AR, Giomi M, Innocenti M, Gallori S, Vincieri FF. HPLC-DAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity. J Pharm Biomed Anal. 2008;46(3):463-70. https://doi.org/10.1016/j.jpba.2007.11.007
Calvo MI. Anti-inflammatory and analgesic activity of the topical preparation of Verbena officinalis L. J Ethnopharmacol. 2006;107(3):380-2.https://doi.org/10.1016/j.jep.2006.03.037
Speroni E, Cervellati R, Costa S, Guerra MC, Utan A, Govoni P, et al. Effects of differential extraction of Verbena officinalis on rat models of inflammation, cicatrization and gastric damage. Planta Med. 2007;73(3):227-35. https://doi.org/10.1055/s-2007-967116
Casanova E, Garcia-Mina JM, Calvo MI. Antioxidant and antifungal activity of Verbena officinalis L. leaves. Plant Foods Hum Nutr. 2008;63(3):93-7. https://doi.org/10.1007/s11130-008-0073-0
De Martino L, D'Arena G, Minervini MM, Deaglio S, Fusco BM, Cascavilla N, De Feo V. Verbena officinalis essential oil and its component citral as apoptotic-inducing agent in chronic lymphocytic leukemia. Int J Immunopathol Pharmacol. 2009;22(4):1097-104. https://doi.org/10.1177/039463200902200426
Shu JC, Liu JQ, Chou GX. A new triterpenoid from Verbena officinalis L. Nat Prod Res. 2013;27(14):1293-7. https://doi.org/10.1080/14786419.2012.733391
Kou WZ, Yang J, Yang QH, Wang Y, Wang ZF, Xu SL, Liu J. Study on in-vivo anti-tumor activity of Verbena officinalis extract. Afr J Tradit Complement Altern Med. 2013;10(3):512-7. https://doi.org/10.4314/ajtcam.v10i3.19
Li L, Leung PS. Use of herbal medicines and natural products: an alternative approach to overcoming the apoptotic resistance of pancreatic cancer. Int J Biochem Cell Biol. 2014;53:224-36. https://doi.org/10.1016/j.biocel.2014.05.021
Modarresi Chahardehi A, Ibrahim D, Fariza Sulaiman S. Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla. Int J Microbiol. 2010;2010:826830. https://doi.org/10.1155/2010/826830
Modarresi Chahardehi A, Ibrahim D, Fariza Sulaiman S. Antioxidant Activity and Total Phenolic Content of Some Medicinal Plants in Urticaceae Family. Journal of Applied Biological Sciences. 2009;2(3):01-5
Wu L-C. Isolation and long-term storage of proteins from tissues and cells using TRIzol reagent. Focus. 1995;17(3):98-100
Xiao S, Zhang Y, Tang X, Yang J, Zhong W, Zhang Y, et al. An improved Trizol method for extracting total RNA from Eleutherococcus senticosus (Rupr. & Maxim.) Maxim leaves. Phytochem Anal. 2024;35(7):1613-9. https://doi.org/10.1002/pca.3404
Les F, Cásedas G, López V. Bioactivity of medicinal plants and extracts. Multidisciplinary Digital Publishing Institute; 2021. p. 634.
Weledji EP, Enoworock G, Mokake M, Sinju M. How Grim is Pancreatic Cancer? Oncol Rev. 2016;10(1):294. https://doi.org/10.4081/oncol.2016.294
Shah SAA, Qureshi NA, Qureshi MZ, Alhewairini SS, Saleem A, Zeb A. Characterization and bioactivities of M. arvensis, V. officinalis and P. glabrum: In-silico modeling of V. officinalis as a potential drug source. Saudi J Biol Sci. 2023;30(6):103646. https://doi.org/10.1016/j.sjbs.2023.103646
Kubica P, Szopa A, Dominiak J, Luczkiewicz M, Ekiert H. Verbena officinalis (Common Vervain) - A Review on the Investigations of This Medicinally Important Plant Species. Planta Med. 2020;86(17):1241-57. https://doi.org/10.1055/a-1232-5758
Platzer M, Kiese S, Tybussek T, Herfellner T, Schneider F, Schweiggert-Weisz U, Eisner P. Radical Scavenging Mechanisms of Phenolic Compounds: A Quantitative Structure-Property Relationship (QSPR) Study. Front Nutr. 2022;9:882458. https://doi.org/10.3389/fnut.2022.882458
Hur SJ, Lee SY, Kim YC, Choi I, Kim GB. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014;160:346-56. https://doi.org/10.1016/j.foodchem.2014.03.112
Afonso AF, Pereira OR, Cardoso SM. Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-Inflammatory and Antitumoral Properties. Antioxidants (Basel). 2020;9(9). https://doi.org/10.3390/antiox9090814
Uno M, Kokuryo T, Yokoyama Y, Senga T, Nagino M. alpha-Bisabolol Inhibits Invasiveness and Motility in Pancreatic Cancer Through KISS1R Activation. Anticancer Res. 2016;36(2):583-9
Jin C-c, Liu X-m, Ma D, Hua X-l, Jin N. Optimization of polysaccharides extracted from Verbena officinalis L and their inhibitory effects on invasion and metastasis of colorectal cancer cells. Tropical Journal of Pharmaceutical Research. 2017;16(10):2387-94. https://doi.org/10.4314/tjpr.v16i10.11
Patel K, Patel DK. Therapeutic Significance of Cornin in Medicine for Their Biological Importance and Pharmacological Activity: An Overview of Iridoid Glycosides of Verbena Officinalis L. Current Functional Foods. 2025;3(1). https://doi.org/10.2174/0126668629277718240101095407
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