Response of host Indian Musa germplasm against artificially induced root lesion nematodes (Pratylenchus coffeae)

A Nithya Devi; K Kumanan; P R Kamalkumaran; R Arunkumar; M. Manikandan; S. Sheeba Joyce Roseleen; V. K. Satya

doi:10.14719/pst.4811

Authors

A Nithya Devi Dr.MSS Agricultural College and Research Institute, Thanjavur, Tamil Nadu, India https://orcid.org/0000-0002-6397-7973
K Kumanan Agricultural College and Research Institute, Pudukkottai, Tamil Nadu, India https://orcid.org/0000-0002-6111-6918
P R Kamalkumaran Agricultural College and Research Institute, Nagapattinam, Tamil Nadu, India https://orcid.org/0000-0002-0386-6788
R Arunkumar Coconut Research Station, Veppankulam, Tamil Nadu, India https://orcid.org/0000-0002-0720-2794
M. Manikandan PAJANCOA & RI, Karaikal, UT of Puducherry, Tamil Nadu, India https://orcid.org/0000-0002-9835-3298
S. Sheeba Joyce Roseleen Horticultural College and Research Institute for Women, Tiruchirappalli, Tamil Nadu, India https://orcid.org/0000-0001-9721-394X
V. K. Satya Horticultural College and Research Institute for Women, Tiruchirappalli, Tamil Nadu, India https://orcid.org/0000-0002-4827-476X

DOI:

https://doi.org/10.14719/pst.4811

Keywords:

artificial inoculation, banana, host response, lesion nematode, pot culture, resistance, tolerance

Abstract

Under greenhouse condition, the host response of fifty-nine banana genotypes (Musa spp.) from the Eumusa section to the root-lesion nematode (Pratylenchus coffeae) was assessed. In a factorial completely randomized design (FCRD) with five replications, healthy banana suckers of the diploid and triploid accessions were planted in cement pots. Uninoculated controls were included in the experiment as comparison to study the sensitivity of the genotypes. Two varieties (Pisang Lilin and Nendran) with known reaction to lesion nematode were also included as reference clones because of their resistance and susceptibility to P. coffeae. Banana accessions maintained in the pots were inoculated with infective juveniles of root-lesion nematode, P. coffeae at 45 days after planting at the rate of 400 nematodes/pot. Ninety days after inoculation, the plants were harvested to observe the response of the different banana genotypes to P. coffeae. Data were recorded on plant growth (plant height, girth at the base, number of standing leaves, number of roots and weight of the root system), root damage assessment (percentage of infected roots and percentage necrosis) and nematode reproduction. When inoculated with nematodes, the reduction in plant growth attributes was relatively higher in genotypes that were susceptible. The reduction in growth characters viz., plants height, number of roots and root fresh weight were maximum in the diploid genotypes Manguthamng (26.80, 27.2, 33.0 %) and Manohar (25.70, 29.2, 35.2 %) and in the triploid genotypes Kaali, Rajthali, Digjowa, Saapkal, Cheenichampa, Dasaman, Borchampa, Jahaji, Manjahaji, Barjahaji and Sabri when inoculated with Pratylenchus coffeae. Even after nematode inoculation, root investigations showed that resistant and tolerant genotypes had greater numbers of roots as well as high fresh and dry weight of roots. Root and soil population of nematodes assessed at 90 days after inoculation indicated very high population buildup of Pratylenchus coffeae (>28) in diploids Manguthamng and Nendrapadathi and in triploids Jahaji, Manjahaji, Saapkal, Borchampa, Therahaw-1163 and 1164, Ankur-I and Bersain. The rate was the lowest (<9) in the diploid genotypes Kanai Bansi, Kechulepa, M.balbisiana, Athiakol, Bhimkol and Aittakola and in the triploids Karthobiumtham and Ankur-II. The results of the experiment showed that the diploids Musa balbisiana (BB), Aittakola (BB), Bhimkol (BB), Kechulepa (BB), Kanai Bansi (AA) and Athiakol (BB) and the triploid genotypes Kachkel (ABB), Karthobiumtham (ABB) and Ankur-II (ABB) were resistant to Pratylenchus coffeae.

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