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Optimizing agro-waste substrates for enhanced enzymatic production in ethnomedicinal mushroom Lentinus squarrosulus Mont.

Authors

K Ganesh Saravanan Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0009-0003-9210-0569
G Thiribhuvanamala Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0001-9160-5491
K Angappan Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0001-5611-1277
T Praveen Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0002-9358-5356
N Revathy Department of Plant Pathology, Tamil Nadu Agricultural University, Madurai 625104, India
R Anandham Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0002-3496-564X
M Jayakanthan Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore 641003, India https://orcid.org/0000-0001-7444-9343

DOI:

https://doi.org/10.14719/pst.4855

Keywords:

agricultural waste, ethnomedicinal mushroom, laccase, mass production, peroxidase

Abstract

The ethnomedicinal subtropical mushroom Lentinus squarrosulus Mont. is a polypore with high wood-degrading potential. This study aimed to collect L. squarrosulus isolates, analyze lignocellulolytic enzymes - Laccase (Lac), Lignin peroxidase (LiP) and Manganese peroxidase (MnP) and evaluate substrates for mass production. Sporophores of Lentinus sp. (Isolate L1 to L5) were collected from different regions of Tamil Nadu and characterized morphologically and molecularly. Qualitative screening tests with guaiacol substrate demonstrated the production of laccase by all the isolates; however, isolate L3 showed maximum reddish-brown zonation. Additionally, isolate L3 produced the highest green colouration on the ABTS (2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) substrate. This confirmed the oxidation of ABTS to ABTS-azine in the presence of laccase, outperforming the other L. squarrosulus isolates. All the isolates tested with Azure-B agar for LiP and MnP showed faint discolouration, suggesting lower secretion of peroxidase enzymes. Quantitative analysis using a lignolysis basal medium also confirmed that isolate L3 secreted Lac predominantly, followed by LiP and MnP enzymes extracellularly. All isolates showed peak Lac activity at pH 4.5, while LiP and MnP production were highest at pH 5.0. The optimal temperature for all lignolytic enzymes was 28±2ºC. Maximum laccase secretion occurred between the 5th - 7th day after inoculation (DAI), with LiP and MnP peaking at 7 DAI. Nine agro-waste substrates were evaluated, with isolate L3 showing the highest biological efficiency on Paddy straw (99.62%), followed by Arecanut sheath (97.43%) and Groundnut shell (95.15%). This study enhances our understanding of L. squarrosulus enzymatic capabilities. It provides insights for mass production, which has strong potential for industrial bioremediation and waste management due to its high laccase production.

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References

Assemie A, Abaya G. The effect of edible mushroom on health and their biochemistry. Inter J Microbiol. 2022;2022(1). https://doi.org/10.1155/2022/8744788

Rathore H, Prasad S, Sharma S. Mushroom nutraceuticals for improved nutrition and better human health: A review. Pharma Nutrition. 2017;5(2):35-46. https://doi.org/10.1016/j.phanu.2017.02.001

Kumar H, Bhardwaj K, Ku?a K, Sharifi?Rad J, Verma R, Machado M, et al. Edible mushrooms’ enrichment in food and feed: A mini review. International Journal of Food Science & Technology. 2022;57(3):1386-98. https://doi.org/10.1111/ijfs.15546

Ugbogu EA, Dike ED, Okoro BC, Adurosakin OE, et al. Lentinus squarrosulus: Nutritional Composition, Phytochemistry, Health-promoting Activities and Toxicity Profile. Food and Humanity. 2024. https://doi.org/10.1016/j.foohum.2024.100296

Narh Mensah DL, Obodai M. Morphological characteristics of mycelia growth of two strains of indigenous medicinal mushroom, Lentinus squarrosulus Mont.(Singer), on solid media. Afr J Agric Res. 2014;9(23):1753-60. https://doi.org/10.5897/AJAR2013.8340

Lau BF, Abdullah N. Bioprospecting of Lentinus squarrosulus Mont., an underutilized wild edible mushroom, as a potential source of functional ingredients: a review. Trends Food Sci Technol 2017;61:116-31. https://doi.org/10.1016/j.tifs.2016.11.017

Ugbogu EA, Akubugwo IE, Ude VC, Gilbert J, Ekeanyanwu B. Toxicological evaluation of phytochemical characterized aqueous extract of wild dried Lentinus squarrosulus (Mont.) mushroom in rats. Toxicol Res. 2019;35:181-90. https://doi.org/10.5487/TR.2019.35.2.181

Fabros JA, Dulay RMR, De Leon AM, Kalaw SP, Reyes RG. Distribution, cultivation, nutritional composition, and bioactivities of Lentinus (Polyporaceae, Basidiomycetes): A review. Curr Res Environ Appl Mycol. 2022;12(1):170-219. https://doi.org/10.5943/cream/12/1/13

Fasiku SA, Bello MA, Odeniyi OA. Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation. Access Microbiology. 2023;5(6):000506-v5. https://doi.org/10.1099/acmi.0.000506.v5

Thornbury M, Sicheri J, Slaine P, Getz LJ, Finlayson-Trick E, Cook J, et al. Characterization of novel lignocellulose-degrading enzymes from the porcupine microbiome using synthetic metagenomics. PLoS One. 2019;14(1):e0209221. https://doi.org/10.1371/journal.pone.0209221

Dashtban M, Schraft H, Syed TA, Qin W. Fungal biodegradation and enzymatic modification of lignin. International journal of biochemistry and molecular biology. 2010;1(1):36.

Ly L, Jitjak W. Biocomposites from agricultural wastes and mycelia of a local mushroom, Lentinus squarrosulus (Mont.) Singer. Open Agriculture. 2022;7(1):634-43. https://doi.org/10.1515/opag-2022-0128

Sánchez C. Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied microbiology and biotechnology. 2010;85:1321-37. https://doi.org/10.1007/s00253-009-2343-7

Balan V, Zhu W, Krishnamoorthy H, Benhaddou D, Mowrer J, Husain H, et al. Challenges and opportunities in producing high-quality edible mushrooms from lignocellulosic biomass in a small scale. Applied Microbiology and Biotechnology. 2022;106(4):1355-74. https://doi.org/10.1007/s00253-021-11749-2

Kosre A, Koreti D, Mahish PK, Chandrawanshi NK. Current perspective of sustainable utilization of agro waste and biotransformation of energy in mushroom. Energy: crises, challenges and solutions. 2021:274-302. https://doi.org/10.1002/9781119741503.ch15

Chang ShuTing CS, Miles PG. Mushrooms: cultivation, nutritional value, medicinal effect and environmental impact. 2004.

Pawlik A, Janusz G, Koszerny J, Ma?ek W, Rogalski J. Genetic diversity of the edible mushroom Pleurotus sp. by amplified fragment length polymorphism. Current Microbiology. 2012;65:438-45.https://doi.org/10.1007/s00284-012-0175-7

Kumar VV, Kirupha SD, Periyaraman P, Sivanesan S. Screening and induction of laccase activity in fungal species and its application in dye decolorization. Afr J Microbiol Res. 2011;5(11):1261-7. https://doi.org/10.5897/AJMR10.894

Thiribhuvanamala G, Kalaiselvi G, Parthasarathy S, Madhavan S, Prakasam V. Extracellular secretion of lignocellulolytic enzymes by diverse white rot basidiomycetes fungi. Ann Phytomed. 2017;6(1):20-9. https://doi.org/10.21276/ap.2017.6.1.4

Asgher M, Wahab A, Bilal M, Iqbal HMN. Lignocellulose degradation and production of lignin modifying enzymes by Schizophyllum commune IBL-06 in solid-state fermentation. Biocatal Agric Biotechnol. 2016;6:195-201. https://doi.org/10.1016/j.bcab.2016.04.003

Thiribhuvanamala G, Parthasarathy S, Ahiladevi P. Schizophyllum commune-A promising source of lignocellulose degrading enzymes suitable for biodegradation. Mushroom Research. 2020;29(1). https://doi.org/10.36036/MR.29.1.2020.108716

Gomez KA, Gomez AA. Statistical procedures for agricultural research: John wiley & sons; 1984.

Isikhuemhen OS, Mikiashvili NA, Adenipekun CO, Ohimain EI, Shahbazi G. The tropical white rot fungus, Lentinus squarrosulus Mont.: lignocellulolytic enzymes activities and sugar release from cornstalks under solid state fermentation. World Journal of Microbiology and Biotechnology. 2012;28:1961-6. https://doi.org/10.1007/s11274-011-0998-6

Mago P, Gunwal I, Singh L, Awasthi D. Commercial production of Oyster mushroom. IOSR Journal of Environmental Science, Toxicology and Food Technology. 2014;8(6):4-9. https://doi.org/10.9790/2402-08630409

Biswas MK, Kuiry S. Yield performance of different species of oyster mushroom (Pleurotus spp.) under the agro-ecological condition of lateritic zone of West Bengal, India. International Journal of Bio-resource and stress management. 2013;4(1):43-6.

Barshteyn V, Krupodorova T. Utilization of agro-industrial waste by higher mushrooms: modern view and trends. Journal of Microbiology, Biotechnology & Food Sciences. 2016;5(6). https://doi.org/10.15414/jmbfs.2016.5.6.563-577

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Published

13-11-2024

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18-11-2024 (2)
13-11-2024 (1)

How to Cite

Saravanan KG, Thiribhuvanamala G, Angappan K, Praveen T, Revathy N, Anandham R, Jayakanthan M. Optimizing agro-waste substrates for enhanced enzymatic production in ethnomedicinal mushroom Lentinus squarrosulus Mont. Plant Sci. Today [Internet]. 2024 Nov. 13 [cited 2024 Nov. 21];. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/4855

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