Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract
DOI:
https://doi.org/10.14719/pst.2019.6.2.532Keywords:
Silver nanoparticle synthesis, bioreduction, Aloe vera gel, characterization, In-vitro antioxidant activityAbstract
Biosynthesis of nanoparticles by using plant extracts is presently under development. The study has been focused on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Aloe vera gel as well as to determine their antioxidant potential. UV-Vis spectrophotometeric analysis showed surface plasmonic resonance (SPR) band at 440 nm, which is specific for AgNPs. The Transmission Electron Microscopy (TEM) revealed that the synthesized AgNPs were spherical in shape with an average particle size of 66.6 nm. Fourier Transform Infrared Spectroscopic (FTIR) analysis of the aqueous extract before and after the synthesis of AgNPs revealed the presence of different functional groups related to phenolic and polyphenolic compounds such as tannins and flavonoids, and other metabolites like proteins, which may be responsible for the synthesis and stabilization of AgNPs. The antioxidant potential of the synthesized AGAgNPs was determined by using 2, 2 Diphenyl-1- Picryl Hydrazyl (DPPH) radical scavenging, metal chelating and reducing power assay. Antioxidant assessment showed enhanced dose dependent antioxidant potential of the synthesized AgNPs as compared to the crude extract, which can gain attention of the pharmaceutical industry for preparation of antioxidants of natural origin as the synthetic ones are suspected to be carcinogenic. Present study also supports the advantages of green method for the nanoparticles synthesis.
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References
1. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002;18(10):872-9. https://doi.org/10.1016/S0899 9007(02)00916-4
2. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertility and sterility. 2003;79(4):829-43. https://doi.org/10.1016/S0015-0282(02)04948-8
3. Lefebvre PP, Malgrange B, Lallemend F, Staecker H, Moonen G, Van De Water TR. Mechanisms of cell death in the injured auditory system: otoprotective strategies. Audiology and Neurotology. 2002;7(3):165-70. https://doi.org/10.1159/000058304
4. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology 2006;5(11).
5. Wong SP, Leong LP, Koh JH. Antioxidant activities of aqueous extracts of selected plants. Food chemistry 2006;99(4):775-83. https://doi.org/10.1016/j.foodchem.2005.07.058
6. Su L, Yin JJ, Charles D, Zhou K, Moore J, Yu LL. Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food chemistry. 2007;100(3):990-7. https://doi.org/10.1016/j.foodchem.2005.10.058
7. Tepe B, Eminagaoglu O, Akpulat HA, Aydin E. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chemistry 2007;100(3):985-9. https://doi.org/10.1016/j.foodchem.2005.10.062
8. Lassen N, Black WJ, Estey T, Vasiliou V. The role of corneal crystallins in the cellular defense mechanisms against oxidative stress. In: Seminars in cell & developmental biology 2008;19:100-112). Academic Press. https://doi.org/10.1016/j.semcdb.2007.10.004
9. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. Journal of agricultural and food chemistry 2002;50(17):4989-93. https://doi.org/10.1021/jf020225o
10. Gülçin ?, Oktay M, Küfrevio?lu Ö?, Aslan A. Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. Journal of Ethnopharmacology 2002;79(3):325-9. https://doi.org/10.1016/S03788741(01)00396-8
11. Singh P, Yadav RJ, Pandey A. Utilization of indigenous systems of medicine & homoeopathy in India. Indian Journal of Medical Research 2005;122(2):137.
12. Kochhar KP. Dietary spices in health and diseases: I. Indian J Physiol Pharmacol. 2008;52(2):106-22.
13. Saxena R, Venkaiah K, Anitha P, Venu L, Raghunath M. Antioxidant activity of commonly consumed plant foods of India: contribution of their phenolic content. International Journal of Food Sciences and Nutrition 2007;58(4):250-60.
14. Ramkumar KM, Manjula C, Sankar L, Suriyanarayanan S, Rajaguru P. Potential in vitro antioxidant and protective effects of Gymnema montanum H. on alloxan-induced oxidative damage in pancreatic ?-cells, HIT-T15. Food and chemical toxicology 2009;47(9):2246-56. https://doi.org/10.1016/j.fct.2009.06.011
15. Saqib S, Hussain Munis MF, Zaman W, Ullah F, Shah SN, Ayaz A, Farooq M, Bahadur S. Synthesis, characterization and use of iron oxide nano particles for antibacterial activity. Microscopy research and technique. 2018;82:415-420. https://doi.org/10.1002/jemt.23182
16. Skousen MV. The ancient egyptian medicine plant: Aloe vera. Cypress (California): Aloe vera Research Inst. 1979;20.
17. Anilakumar KR, Sudarshanakrishna KR, Chandramohan G, Ilaiyaraja N, Khanum F, Bawa AS. Effect of Aloe vera gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rats. Indian J Exp Biol. 2010;48(8):837-42.
18. Saritha V, Anilakumar KR, Khanum F. Antioxidant and antibacterial activity of Aloe vera gel extracts. International Jurnal of Pharmaceutical & Biological Archives 2010;1(4):376-84.
19. Niko N. Inhibitory effects of Aloe vera gel aqueous extract and L. casei against E. coli in yoghurt. Journal of Biology and Today's World 2016;5(9):157-62. https://doi.org/10.15412/J.JBTW.01050901
20. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture 1977;1:28(1):49-55.
21. Bibi G, Ullah N, Muazzam AG, Mannan A, Mirza B. Phytochemical evaluation of naturally growing Aster thomsonii plant species. J Pharm Harb Form. 2012;2:33-9.
22. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181(4617):1199.
23. Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. Journal of Agricultural and Food Chemistry 1994;42(3):629-32. https://doi.org/10.1021/jf00039a005
24. Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and food Chemistry 1990;38(3):674-7. https://doi.org/10.1021/jf00093a019
25. Oraiza M. Studies on product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 1986;44:307-15.
26. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. Journal of Agricultural and Food Chemistry 2002;50(8):2454-8. https://doi.org/10.1021/jf011369q
27. Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and surfaces B: Biointerfaces 2003;28(4):313-8. https://doi.org/10.1016/S09277765(02)00174-1
28. Mittal AK, Kaler A, Banerjee UC. Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Flower Extract of Rhododendron dauricum. Nano Biomedicine & Engineering 2012;4(3).
29. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids and Surfaces B: Biointerfaces 2010;81(1):81-6. https://doi.org/10.1016/j.colsurfb.2010.06.029
30. Interpretation of infrared spectra. Slideshare. https://www.slideshare.net/aksharanaidu967/keerthi-ppt (Accessed 2018-11-18)
31. Sivaraman SK, Elango I, Kumar S, Santhanam V. A green protocol for room temperature synthesis of silver nanoparticles in seconds. Current Science 2009;97(7):1055-9.
32. Phull AR, Abbas Q, Ali A, Raza H, Zia M, Haq IU. Antioxidant, cytotoxic and antimicrobial activities of green synthesized silver nanoparticles from crude extract of Bergenia ciliata. Future Journal of Pharmaceutical Sciences 2016;2(1):31-6. https://doi.org/10.1016/j.fjps.2016.03.001
33. Baumann J. Prostaglandin synthetase inhibiting O_2-radical scavenging properties of some flavonoids and related phenolic compounds. Naunyn-Schmiedebergs Arch Pharmacol. 1979;308:27-32.
34. Soler?Rivas C, Espín JC, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques 2000;11(5):330-8. https://doi.org/10.1002/1099-1565(200009/10)11:5%3C330::aid-pca534%3E3.3.co;2-7
35. Tanaka M, Kuie CW, Nagashima YAA, Taguchi T. Application of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi, 1988;54(8):1409-1414. https://doi.org/10.2331/suisan.54.1409
36. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of agricultural and food chemistry 1995;43(7):1813-9. https://doi.org/10.1021/jf00055a012
37. Duh PD. Antioxidant activity of burdock (Arctium lappa Linné): Its scavenging effect on free?radical and active oxygen. Journal of the American Oil Chemists' Society 1998;75(4):455-61. https://doi.org/10.1007/s11746-998-0248-8
38. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of agricultural and food chemistry 2001;49(11):5165-70. https://doi.org/10.1021/jf010697n
39. Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Applied Nanoscience 2016;;6(5):755-66.
40. Patil Shriniwas P. Antioxidant, antibacterial and cytotoxic potential of silver nanoparticles synthesized using terpenes rich extract of Lantana camara L. leaves. Biochemistry and biophysics reports 2017;10:76. https://doi.org/10.1016/j.bbrep.2017.03.002
41. Otunola GA, Afolayan AJ. In vitro antibacterial, antioxidant and toxicity profile of silver nanoparticles green-synthesized and characterized from aqueous extract of a spice blend formulation. Biotechnology & Biotechnological Equipment 2018;32(3):724-33.
2. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertility and sterility. 2003;79(4):829-43. https://doi.org/10.1016/S0015-0282(02)04948-8
3. Lefebvre PP, Malgrange B, Lallemend F, Staecker H, Moonen G, Van De Water TR. Mechanisms of cell death in the injured auditory system: otoprotective strategies. Audiology and Neurotology. 2002;7(3):165-70. https://doi.org/10.1159/000058304
4. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology 2006;5(11).
5. Wong SP, Leong LP, Koh JH. Antioxidant activities of aqueous extracts of selected plants. Food chemistry 2006;99(4):775-83. https://doi.org/10.1016/j.foodchem.2005.07.058
6. Su L, Yin JJ, Charles D, Zhou K, Moore J, Yu LL. Total phenolic contents, chelating capacities, and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food chemistry. 2007;100(3):990-7. https://doi.org/10.1016/j.foodchem.2005.10.058
7. Tepe B, Eminagaoglu O, Akpulat HA, Aydin E. Antioxidant potentials and rosmarinic acid levels of the methanolic extracts of Salvia verticillata (L.) subsp. verticillata and S. verticillata (L.) subsp. amasiaca (Freyn & Bornm.) Bornm. Food Chemistry 2007;100(3):985-9. https://doi.org/10.1016/j.foodchem.2005.10.062
8. Lassen N, Black WJ, Estey T, Vasiliou V. The role of corneal crystallins in the cellular defense mechanisms against oxidative stress. In: Seminars in cell & developmental biology 2008;19:100-112). Academic Press. https://doi.org/10.1016/j.semcdb.2007.10.004
9. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. Journal of agricultural and food chemistry 2002;50(17):4989-93. https://doi.org/10.1021/jf020225o
10. Gülçin ?, Oktay M, Küfrevio?lu Ö?, Aslan A. Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. Journal of Ethnopharmacology 2002;79(3):325-9. https://doi.org/10.1016/S03788741(01)00396-8
11. Singh P, Yadav RJ, Pandey A. Utilization of indigenous systems of medicine & homoeopathy in India. Indian Journal of Medical Research 2005;122(2):137.
12. Kochhar KP. Dietary spices in health and diseases: I. Indian J Physiol Pharmacol. 2008;52(2):106-22.
13. Saxena R, Venkaiah K, Anitha P, Venu L, Raghunath M. Antioxidant activity of commonly consumed plant foods of India: contribution of their phenolic content. International Journal of Food Sciences and Nutrition 2007;58(4):250-60.
14. Ramkumar KM, Manjula C, Sankar L, Suriyanarayanan S, Rajaguru P. Potential in vitro antioxidant and protective effects of Gymnema montanum H. on alloxan-induced oxidative damage in pancreatic ?-cells, HIT-T15. Food and chemical toxicology 2009;47(9):2246-56. https://doi.org/10.1016/j.fct.2009.06.011
15. Saqib S, Hussain Munis MF, Zaman W, Ullah F, Shah SN, Ayaz A, Farooq M, Bahadur S. Synthesis, characterization and use of iron oxide nano particles for antibacterial activity. Microscopy research and technique. 2018;82:415-420. https://doi.org/10.1002/jemt.23182
16. Skousen MV. The ancient egyptian medicine plant: Aloe vera. Cypress (California): Aloe vera Research Inst. 1979;20.
17. Anilakumar KR, Sudarshanakrishna KR, Chandramohan G, Ilaiyaraja N, Khanum F, Bawa AS. Effect of Aloe vera gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rats. Indian J Exp Biol. 2010;48(8):837-42.
18. Saritha V, Anilakumar KR, Khanum F. Antioxidant and antibacterial activity of Aloe vera gel extracts. International Jurnal of Pharmaceutical & Biological Archives 2010;1(4):376-84.
19. Niko N. Inhibitory effects of Aloe vera gel aqueous extract and L. casei against E. coli in yoghurt. Journal of Biology and Today's World 2016;5(9):157-62. https://doi.org/10.15412/J.JBTW.01050901
20. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture 1977;1:28(1):49-55.
21. Bibi G, Ullah N, Muazzam AG, Mannan A, Mirza B. Phytochemical evaluation of naturally growing Aster thomsonii plant species. J Pharm Harb Form. 2012;2:33-9.
22. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181(4617):1199.
23. Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. Journal of Agricultural and Food Chemistry 1994;42(3):629-32. https://doi.org/10.1021/jf00039a005
24. Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and food Chemistry 1990;38(3):674-7. https://doi.org/10.1021/jf00093a019
25. Oraiza M. Studies on product of browning reaction prepared from glucosamine. Japanese Journal of Nutrition 1986;44:307-15.
26. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. Journal of Agricultural and Food Chemistry 2002;50(8):2454-8. https://doi.org/10.1021/jf011369q
27. Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and surfaces B: Biointerfaces 2003;28(4):313-8. https://doi.org/10.1016/S09277765(02)00174-1
28. Mittal AK, Kaler A, Banerjee UC. Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Flower Extract of Rhododendron dauricum. Nano Biomedicine & Engineering 2012;4(3).
29. Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids and Surfaces B: Biointerfaces 2010;81(1):81-6. https://doi.org/10.1016/j.colsurfb.2010.06.029
30. Interpretation of infrared spectra. Slideshare. https://www.slideshare.net/aksharanaidu967/keerthi-ppt (Accessed 2018-11-18)
31. Sivaraman SK, Elango I, Kumar S, Santhanam V. A green protocol for room temperature synthesis of silver nanoparticles in seconds. Current Science 2009;97(7):1055-9.
32. Phull AR, Abbas Q, Ali A, Raza H, Zia M, Haq IU. Antioxidant, cytotoxic and antimicrobial activities of green synthesized silver nanoparticles from crude extract of Bergenia ciliata. Future Journal of Pharmaceutical Sciences 2016;2(1):31-6. https://doi.org/10.1016/j.fjps.2016.03.001
33. Baumann J. Prostaglandin synthetase inhibiting O_2-radical scavenging properties of some flavonoids and related phenolic compounds. Naunyn-Schmiedebergs Arch Pharmacol. 1979;308:27-32.
34. Soler?Rivas C, Espín JC, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques 2000;11(5):330-8. https://doi.org/10.1002/1099-1565(200009/10)11:5%3C330::aid-pca534%3E3.3.co;2-7
35. Tanaka M, Kuie CW, Nagashima YAA, Taguchi T. Application of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi, 1988;54(8):1409-1414. https://doi.org/10.2331/suisan.54.1409
36. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of agricultural and food chemistry 1995;43(7):1813-9. https://doi.org/10.1021/jf00055a012
37. Duh PD. Antioxidant activity of burdock (Arctium lappa Linné): Its scavenging effect on free?radical and active oxygen. Journal of the American Oil Chemists' Society 1998;75(4):455-61. https://doi.org/10.1007/s11746-998-0248-8
38. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of agricultural and food chemistry 2001;49(11):5165-70. https://doi.org/10.1021/jf010697n
39. Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Applied Nanoscience 2016;;6(5):755-66.
40. Patil Shriniwas P. Antioxidant, antibacterial and cytotoxic potential of silver nanoparticles synthesized using terpenes rich extract of Lantana camara L. leaves. Biochemistry and biophysics reports 2017;10:76. https://doi.org/10.1016/j.bbrep.2017.03.002
41. Otunola GA, Afolayan AJ. In vitro antibacterial, antioxidant and toxicity profile of silver nanoparticles green-synthesized and characterized from aqueous extract of a spice blend formulation. Biotechnology & Biotechnological Equipment 2018;32(3):724-33.
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Published
15-05-2019
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Sohal JK, Saraf A, Shukla K, Shrivastava M. Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract. Plant Sci. Today [Internet]. 2019 May 15 [cited 2024 Nov. 21];6(2):208-17. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/532
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