Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract

Authors

  • Jasmeet Kaur Sohal Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India
  • Ashish Saraf Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India
  • Kamlesh Shukla SoS Biotechnology, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
  • Meghna Shrivastava Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India

DOI:

https://doi.org/10.14719/pst.2019.6.2.532

Keywords:

Silver nanoparticle synthesis, bioreduction, Aloe vera gel, characterization, In-vitro antioxidant activity

Abstract

Biosynthesis of nanoparticles by using plant extracts is presently under development. The study has been focused on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Aloe vera gel as well as to determine their antioxidant potential. UV-Vis spectrophotometeric analysis showed surface plasmonic resonance (SPR) band at 440 nm, which is specific for AgNPs. The Transmission Electron Microscopy (TEM) revealed that the synthesized AgNPs were spherical in shape with an average particle size of 66.6 nm. Fourier Transform Infrared Spectroscopic (FTIR) analysis of the aqueous extract before and after the synthesis of AgNPs revealed the presence of different functional groups related to phenolic and polyphenolic compounds such as tannins and flavonoids, and other metabolites like proteins, which may be responsible for the synthesis and stabilization of AgNPs. The antioxidant potential of the synthesized AGAgNPs was determined by using 2, 2 Diphenyl-1- Picryl Hydrazyl (DPPH) radical scavenging, metal chelating and reducing power assay. Antioxidant assessment showed enhanced dose dependent antioxidant potential of the synthesized AgNPs as compared to the crude extract, which can gain attention of the pharmaceutical industry for preparation of antioxidants of natural origin as the synthetic ones are suspected to be carcinogenic. Present study also supports the advantages of green method for the nanoparticles synthesis.

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Author Biographies

Jasmeet Kaur Sohal, Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India

MATS School of Biological and Chemical Sciences, MATS University

Research Scholar

Ashish Saraf, Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India

MATS School of Biological and Chemical Sciences, MATS University

Head of the Department

Meghna Shrivastava, Faculty of Biological and Chemical Sciences, MATS University, Raipur, Chhattisgarh, India

MATS School of Biological and Sciences, MATS University

Research Scholar

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Published

15-05-2019

How to Cite

1.
Sohal JK, Saraf A, Shukla K, Shrivastava M. Determination of antioxidant potential of biochemically synthesized silver nanoparticles using Aloe vera gel extract. Plant Sci. Today [Internet]. 2019 May 15 [cited 2024 Nov. 4];6(2):208-17. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/532

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Research Articles