Evaluation of different passion fruit genotypes based on morphological, quantitative traits and molecular marker (ISSR)
DOI:
https://doi.org/10.14719/pst.5420Keywords:
clustering, correlation, ISSR marker, passion fruit, Principal Compound Analysis (PCA)Abstract
Passion fruit (Passiflora spp.), an underutilized and unexplored fruit crop, holds significant potential for improving nutritional health in tropical and subtropical regions. This study aimed to characterize various genotypes of passion fruit including yellow (G1, G3, G5), purple (G2, G4, G10), sweet (G6, G7, G9) and giant (G8), collected from different regions in Tamil Nadu, India, based on morphological, yield and biochemical traits. A comprehensive evaluation of morphological, yield and quality traits, combined with advanced statistical analysis, including correlation studies and principal component analysis (PCA), identified genotypes with desirable traits, offering valuable resources for breeding programs to improve passion fruit cultivation. The analysis included biometric data, yield performance and quality traits, complemented by molecular diversity assessment using ISSR markers. Significant variation was observed across morphological and biochemical traits; genotype G8 showed the highest fruit weight and juice content, while genotype G7 had the highest total soluble solids and sugar content. Principal component analysis (PCA) and hierarchical clustering revealed distinct groupings, providing insights into their genetic diversity and potential for future crop improvement. These findings suggest that specific genotypes could be strategically targeted for breeding programs to enhance yield, fruit quality and resilience in passion fruit cultivation.
Downloads
References
Vieira MLC, Zirlane Portugal Costa, Alessandro Mello Varani, Mariela Analia Sader, Luiz Augusto Cauz-Santos, Helena Augusto Giopatto, Alina Carmen Egoávil del Reátegui, Hélène Bergès, Claudia Barros Monteiro-Vitorello, Marcelo Carnier Dornelas, Andrea Pedrosa-Harand. The passion fruit genome. In: Chapman, M.A. (eds) Underutilised Crop Genomes. Compendium of Plant Genomes. Springer. 2022:131–48. https://doi.org/10.1007/978-3-031-00848-1_8
Pereira AD, Corrêa RX, Oliveira AC. Molecular genetic diversity and 749 differentiation of populations of ‘somnus’ passion fruit trees (Passiflora setacea 750 DC): Implications for conservation and pre-breeding. Biochemical Systematics 751 and Ecolog. 2015;59:12–21. https://doi.org/10.1016/j.bse.2014.12.020.
Diaz RO, Moreno L, Pinilla R, Carrillo W, Melgarejo LM, Martinez O, Hernandez MS. Postharvest behaviour of purple passion fruit in tend bags during low temperature storage. Acta hortic. 2012;934:727–32. https://doi.org/10.17660/ActaHortic.2012.934.95
Miroddi M, Calapai G, Navarra M, Minciullo PL, Gangemi S. Passiflora incarnata L.: ethnopharmacology, clinical application, safety and evaluation of clinical trials. Journal of ethnopharmacology. 2013;150(3):791–804. https://doi.org/10.1016/j.jep.2013.09.047
Silva FHL, Muñoz PR, Vincent CI, Viana AP. Generating relevant information for breeding Passiflora edulis: genetic parameters and population structure. Euphytica. 2016;208:609–19. https://doi.org/10.1007/s10681-015-1616-8
Cerqueira-Silva CBM, Jesus ON, Oliveira EJ. Characterization and selection of passion fruit (yellow and purple) accessions based on molecular markers and disease reactions for use in breeding programs. Euphytica. 2015;202:345–59. https://doi.org/10.1007/s10681-014-1235-9
Devi Ramaiya S, Bujang JS, Zakaria MH, King WS, Shaffiq Sahrir MA. Sugars, ascorbic acid, total phenolic content and total antioxidant activity in passion fruit (Passiflora) cultivars. J Sci Food Agric. 2013;93(5):1198–205. https://doi.org/10.1002/jsfa.5876
Fonseca AMA, Geraldi MV, Junior MRM, Silvestre AJD, Rocha SM. Purple passion fruit (Passiflora edulis f. edulis): A comprehensive review on the nutritional value, phytochemical profile and associated health effects. Food Res Int. 2022;160:111665. https://doi.org/10.1016/j.foodres.2022.111665
Cerqueira-Silva CBM, Faleiro FG, de Jesus ON, dos Santos ESL, de Souza AP. Passion Fruit (Passiflora spp.) Breeding. In: Al-Khayri J, Jain S, Johnson D. (eds) Advances in Plant Breeding Strategies: Fruits. Springer; 2018. https://doi.org/10.1007/978-3-319-91944-7_22
Cerqueira-Silva CBM, Conceição LDHCS, Souza AP, Corrêa RX. A history of passion fruit woodiness disease with emphasis on the current situation in Brazil and prospects for Brazilian passion fruit cultivation. Eur J Plant Pathol. 2014;139:261–70. https://doi.org/10.1007/s10658-014-0391-z
Best DJ, Roberts DE. Journal of the Royal Statistical Society. Series C. Applied Statistics. Oxford University Press. 1975;24(3):377–9. https://doi.org/10.2307/2347111
Ward JH. Hierarchical Grouping to Optimize an Objective Function. Journal of the American Statistical Association. 1963;58(301), 236–44. https://doi.org/10.1080/01621459.1963.10500845
Roldan-Ruiz I, Dendauw J, Vanbockstaele E, Depicker A, De Loose M. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed. 2000;6:125–34. https://doi.org/10.1023/A:1009680614564
Lewontin RC. The Apportionment of Human Diversity. In: Dobzhansky T, Hecht MK, Steere WC. (eds) Evol Biol. Springer, New York; 1972. https://doi.org/10.1007/978-1-4684-9063-3_14.
Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA. 1973;70(12):3321–23. https://doi.org/10.1073/pnas.70.12.3321
Peakall R, Smouse PE. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update. Bioinformatics. 2012;28(19):2537–9. https://doi.org/10.1093/bioinformatics/bts460
Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 1978;89(3):583–90. https://doi.org/10.1093/genetics/89.3.583
Perrier X, Jacquemoud-Collet JP. DARwin software; 2006. http://darwin.cirad.fr/
Bruckner CH, Casali VWD, de Moraes CF, Regazzi AJ, da Silva EAM. Self incompatibility in passion fruit (Passiflora edulis Sims). Acta Hortic. 1995;370:45–57. https://doi.org/10.17660/ActaHortic.1995.370.7
Joy PP. Passion fruit (Passiflora edulis Sims): Passifloraceae. Technical Bulletin, Pineapple Research Station, Kerala Agricultural University, Vazhakulam, Kerala, India; 2010.
Deshmukh NA, Patel RK, Okram S, Rymbai H, Roy SS, Jha AK. Passion fruit (Passiflora spp.) In: S. N. Ghosh, A. Singh and A. Thakur (eds.) Underutilized fruit crops: importance and cultivation. JAYA Publishing House. 2017;979–1005.
Aizza LCB, Sawaya ACHF, Dornelas MC. Identification of anthocyanins in the corona of two species of Passiflora and their hybrid by ultra-high performance chromatography with electrospray ionization tandem mass spectrometry (UHPLC-ESI MS/MS). Biochem. Systematics Ecol. 2019;85:60–67. https://doi.org/10.1016/J.BSE.2019.05.003
Joseph AV, Sobhana A, Joseph J, Bhaskar J, Vikram H, Sankar SJ. Performance evaluation of passion fruit (Passiflora edulis Sims.) genotypes. JTA. 2022;59(2). https://jtropag.kau.in/index.php/ojs2/article/view/933
Patel RK, Akath Singh, Jai Prakash, Amit Nath, Bidyut C, Deka. Physico-biochemical changes during fruit growth, development and maturity in passion fruit genotypes. Indian J. Hort. 2014;71(4).
Charan SM, Gomez S, Sheela KB, Joseph PM, Sruthi CV. Quality characteristics and antioxidant activity of passion fruit (Passiflora edulis Sims.) accessions. Ind. J. Hortic. 2018;75:185–90. https://doi.org/10.5958/0974-0112.2018.00034.8
Septembre-Malaterre Axelle, Stanisla, Giovédie, Douraguia Elisabeth, Gonthier Marie-Paule. Evaluation of nutritional and antioxidant properties of the tropical fruits banana, litchi, mango, papaya, passion fruit and pineapple cultivated in Réunion French Island. Food Chemistry. 2016;212:225–33. https://doi.org/10.1016/j.foodchem.2016.05.147
Pongener A, Sagar V, Pal RK, Asrey R, Sharma RR, Singh SK. Physiological and quality changes during postharvest ripening of purple passion fruit (Passiflora edulis Sims). Fruits. 2014;69(1):19–30. https://doi.org/10.1051/fruits/2013097
Weber D, Fachinello JC, Saavedra del Aguila J. Correlations between production and quality characteristics of the yellow passion fruit selection' Ovalado Grande'in southern Brazil. Acta Hortic. 2017. http://dx.doi.org/10.17660/ActaHortic.2017.1178.3
Beena VL, Beevy SS. Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling. The Nucl. 2015;58(2):101–6. https://doi.org/10.1007/s13237-015-0137-0
Haseena Bai N and Suhara Beevy S. Characterization of Intraspecific F1 Hybrids of Momordica charantia L. Based on Morphology, Cytology and Palynology. Cytologia. 2012;77(3):301–10. http://dx.doi.org/10.1508/cytologia.77.301
Singh HP, Raigar OP, Chahota, RK. Estimation of genetic diversity and its exploitation in plant breeding. Bot. Rev. 2022;88:413–35. https://doi.org/10.1007/s12229-021-09274-y
Ramos, Jailton, Lima, Vera Lucia, Lima, Geovani, Pereira, Mariana, Rodrigues da Silva, André, Nunes, Kheila. Growth and quality of passion fruit seedlings under salt stress and H2O2 in plant leaves. Comunicata Scientiae. 2022;13. http://dx.doi.org/10.14295/cs.v13.3393
Tripathi PC. Passion Fruit. (In) Peter KV (Ed). Horticultural Crops of high nutraceutical values. Brillion Publishing New Delhi. 2018; 245–70.
Silva FHL, Muñoz PR, Vincent CI. Generating relevant information for breeding Passiflora edulis: genetic parameters and population structure. Euphytica. 2016;208:609–19. https://doi.org/10.1007/s10681-015-1616-8
Santos CL, Viana AP, Freitas MSM, Carvalho AJC, Rodrigues DL. Relationship between Yield and Fruit Quality of Passion Fruit C03 Progenies under Different Nutritional Levels. Revista Brasileira de Fruticultura. 2017;39. https://doi.org/10.1590/0100-29452017691
dos Santos Amorim J, Souza MM, Viana AJC. Cytogenetic, molecular and morphological characterization of Passiflora capsularis L. and Passiflora rubra L. Plant Syst Evol. 2014;300, 1147–62. https://doi.org/10.1007/s00606-013-0952-1
Silva TV, Resende ED, Viana AP, Pereira SMF, Carlos CA, Vitorazi L. Determination of peel colour scale and juice yield of yellow passion fruits in different harvest seasons. Rev. Bras. Frutic. 2008;30(4): 880–4. http://dx.doi.org/10.1590/S0100-29452008000400007
Perez JAO, Forero Pinto LE, MacDougal JM. Morphological analysis reveals a new species of Passiflora subgenus Decaloba (Passifloraceae): Passiflora quimbayensis, an endemic species from Colombia. Systematic Bot. 2018;43(1):231–9. https://doi.org/10.1600/036364418X696923
Viana AJC, Souza MM, Araújo IS. Genetic diversity in Passiflora species determined by morphological and molecular characteristics. Biol Plant. 2010;54:535–8. https://doi.org/10.1007/s10535-010-0094-z
Lawinscky PR, Souza MM, de Oliveira Belo G. Morphological characterization and genetic diversity in Passiflora alata Curtis and P. cincinnata Mast. (Passifloraceae). Braz. J. Bot. 2014;37:261–72. https://doi.org/10.1007/s40415-014-0077-y
Espinosa DS, Melgarejo LM, Hernández MS, Melo SE, Fernández-Trujillo JP. Physiological and biochemical characterization of sweet granadilla (Passiflora ligularis J.) at different locations. Acta Hortic. 2018;1194:1459–64.
https://doi.org/10.17660/ActaHortic.2018.1194.204
Arjona, Harvey E, Frank B, Matta. “Postharvest Quality of Passion Fruit as Influenced by Harvest Time and Ethylene Treatment”. Hortscience. 1991;26:1297–8. https://doi.org/10.21273/HORTSCI.26.10.1297
Rodriguez GD, Garcia MC, Plaza M, Marina ML. Revalorization of Passiflora species peels as a sustainable source of antioxidant phenolic compounds. Sci. Total Environ. 2019;696:30–34. https://doi.org/10.1016/j.scitotenv.2019.134030
Loizzo MR, Lucci P, Nunez O, Tundis R, Balzano M, Frega NG, Lanfranco C, Sabrina M, Daria F, Encarnacion M, Pacetti D. Native colombian fruits and their by-products: Phenolic profile, antioxidant activity and hypoglycaemic potential. Foods. 2019;8(3):89. https://doi.org/10.3390/foods8030089
Silva DJK, Cazarin CBB, Batista AG, Marostica M. Effects of passion fruit (Passiflora edulis) by product intake in antioxidant status of Wistar rats tissues. Food Sci. Technol. 2014;59:1213–9. https://doi.org/10.1016/J.LWT.2014.06.060
Ballesteros VD, Alvarez-Rivera G, Ibanez E, Parada-Alfonso F, Cifuentes A. Integrated strategy for the extraction and profiling of bioactive metabolites from Passiflora mollissima seeds combining pressurized liquid extraction and gas/liquid chromatography–high resolution mass spectrometry. J. Chromatography A. 2019;1595:144–57. https://doi.org/10.1016/j.chroma.2019.02.031
Reis LCRD, Facco EMP, Salvador M, Flores SH, De Oliveira Rios A. Antioxidant potential and physicochemical characterization of yellow, purple and orange passion fruit. J. Food Sci. Technol. 2018;55(7):2679–91. https://doi.org/10.1007/s13197-018-3190-2
Pertuzatti PB, Sganzerla M, Jacques AC, Barcia MT, Zambiazi RC. Carotenoids, tocopherols and ascorbic acid content in yellow passion fruit (Passiflora edulis) grown under different cultivation systems. Food Sci. Technol. 2015;64(2015):259–63. https://doi.org/10.1016/J.LWT.2015.05.031
Martins MR, de Oliveira JC, Di Mauro AO, da Silva PC. Evaluation of passion fruit populations (Passiflora alata Curtis) obtained from open pollination. Rev. Bras. Frutic. 2003;25:111–4.
Fajardo D, Angel F, Grum M. Genetic variation analysis of the genus Passiflora L. using RAPD markers. Euphytica. 1998;101:341–7. https://doi.org/10.1023/A:1018395229851
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 S Snekha, I Geethalakshmi, S Saraswathy, N Malini, K Sakthivel, S Geethanjali
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
