Exploring the potential of amine-functionalized mesoporous silica nanocarrier to conjugate guide RNA for gene delivery
DOI:
https://doi.org/10.14719/pst.5470Keywords:
bioconjugation, mesoporous silica, sgRNA, sol-gel, sugarcane bagasseAbstract
Sugarcane is a key cash crop, with nations such as Brazil, India, and China being the leading producers. The trade of sugar and related products contributes significantly to these economies. Sugarcane is the primary feedstock for producing sugar and ethanol in the sugarcane processing industry, yielding sugarcane bagasse fly ash (SBFA) as a byproduct. This byproduct comes primarily from the combustion of bagasse (the fibrous residue after juice extraction) and contains silica (SiO2), alumina (Al2O3), and other minerals. The use of sugarcane waste materials reduces the need for new raw resources and lowers the environmental impact. Turning waste into valuable nanoparticles also helps manage waste better and reduces the amount that is burnt or dumped in landfills. The fly ash was treated with an alkaline solution (e.g., sodium hydroxide) to extract silica. This process helps dissolve other minerals, leaving a silica-rich residue. After extraction, the silica was purified through acid washing to remove impurities. Mesoporous silica nanoparticles were created using the sol-gel method and 3-aminopropyltriethoxysilane (APTES) was added to them functionally to conjugate sgRNA. The synthesized mesoporous silica nanoparticles were characterized for size and shape, functional groups, and crystallinity using various instruments such as Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering, Brunauer-Emmett-Teller (BET) Analyzer, and X-Ray Diffractometer (XRD), respectively. Furthermore, synthesized mesoporous silica nanoparticles were bioconjugated with sgRNA of OsEPF1, a gene negative regulation of stomatal development, which in turn, helps reduce yield loss in rice due to drought and heat stress.
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Bharti C, Nagaich U, Pal AK, Gulati N. Mesoporous silica nanoparticles in target drug delivery system: a review. International Journal of Pharmaceutical Investigation. 2015;5(3):124-33. https://doi.org/10.4103/2230-973x.160844
Frickenstein AN, Hagood JM, Britten CN, Abbott BS, McNally MW, Vopat CA, et al. Mesoporous silica nanoparticles: properties and strategies for enhancing clinical effect. Pharmaceutics. 2021;13(4):570.https://doi.org/10.3390/pharmaceutics13040570
Nik AB, Zare H, Razavi S, Mohammadi H, Ahmadi PT, Yazdani N, et al. Smart drug delivery: capping strategies for mesoporous silica nanoparticles. Microporous and Mesoporous Materials. 2020;299:110115. https://doi.org/10.1016/j.micromeso.2020.110115
Gosiamemang T, Heng JY. Sodium hydroxide catalysed silica sol-gel synthesis: Physicochemical properties of silica nanoparticles and their post-grafting using C8 and C18 alkylorganosilanes. Powder Technology. 2023;417:118237. https://doi.org/10.1016/j.powtec.2023.118237
Ghaferi M, Koohi MEM, Raza A, Al Harthi S, Ebrahimi SH, Alavi SE. Mesoporous silica nanoparticles: synthesis methods and their therapeutic use-recent advances. Journal of Drug Targeting. 2021;29(2):131-54. https://doi.org/10.1080/1061186x.2020.1812614
Wang Y, Yang C, Wang X, Zhang S, Wang S, Wu D, et al. Determination of free fatty acids in edible oil based on hollow mesoporous silica nanoparticles. Food Chemistry. 2024;443:138561. https://doi.org/10.1016/j.foodchem.2024.138561
Purcar V, R?di?oiu V, Nichita C, B?lan A, R?di?oiu A, C?pr?rescu S, et al. Preparation and characterization of silica nanoparticles and of silica-gentamicin nanostructured solution obtained by microwave-assisted synthesis. Materials. 2021;14(8):2086. https://doi.org/10.3390/ma14082086
Wu SH, Mou CY, Lin HP. Synthesis of mesoporous silica nanoparticles. Chemical Society Reviews. 2013;42(9):3862-75. https://doi.org/10.1039/C3CS35405A
Singh B, Na J, Konarova M, Wakihara T, Yamauchi Y, Salomon C, et al. Functional mesoporous silica nanomaterials for catalysis and environmental applications. Bulletin of the Chemical Society of Japan. 2020;93(12):1459-96. https://doi.org/10.1246/bcsj.20200136
Abu-Dief AM, Alsehli M, Al-Enizi A, Nafady A. Recent advances in mesoporous silica nanoparticles for targeted drug delivery applications. Current Drug Delivery. 2022;19(4):436-50. https://doi.org/10.2174/1567201818666210708123007
Heidari R, Assadollahi V, Khosravian P, Mirzaei SA, Elahian F. Engineered mesoporous silica nanoparticles, new insight nanoplatforms into effective cancer gene therapy. Inter J Biol Macromol. 2023;253(Pt 4):127060. https://doi.org/10.1016/j.ijbiomac.2023.127060
Guo M, Han J, Ran Q, Zhao M, Liu Y, Zhu G, et al. Multifunctional integrated electrochemical sensing platform based on mesoporous silica nanoparticles decorated single-wall carbon nanotubes network for sensitive determination of gallic acid. Ceramics International. 2023;49(23):37549-60. https://doi.org/10.1016/j.ceramint.2023.09.081
Alotaibi KM. Mesoporous silica nanoparticles modified with stimuli-responsive polymer brush as an efficient adsorbent for chlorophenoxy herbicides removal from contaminated water. Inter J Environ Anal Chem. 2023;103(16):3669-82. https://doi.org/10.1080/03067319.2021.1907362
September LA, Kheswa N, Seroka NS, Khotseng L. Green synthesis of silica and silicon from agricultural residue sugarcane bagasse ash-a mini review. RSC Advances. 2023;13(2):1370-80. https://doi.org/10.1039/D2RA07490G
Sarkar J, Mridha D, Sarkar J, Orasugh JT, Gangopadhyay B, Chattopadhyay D, et al. Synthesis of nanosilica from agricultural wastes and its multifaceted applications: a review. Biocatal Agri Biotechnol. 2021;37:102175. https://doi.org/10.1016/j.bcab.2021.102175
Rathnasamy SA, Kambale R, Elangovan A, Mohanavel W, Shanmugavel P, Ramasamy G, et al. Altering stomatal density for manipulating transpiration and photosynthetic traits in rice through CRISPR/Cas9 mutagenesis. Curr Issues Mol Biol. 2023;45(5):3801-14. https://doi.org/10.3390/cimb45050245
Sasidhar B, Malathi P, Rahale CS, Gnanachitra M, Sharmila D. Synthesis and characterization of mesoporous nanosilica derived from rice husk ash. Inter J Environ Climate Change. 2023;13(10):3526-32. https://doi.org/10.3390/cimb45050245
Wang D, Xu X, Zhang K, Sun B, Wang L, Meng L, et al. Codelivery of doxorubicin and MDR1-siRNA by mesoporous silica nanoparticles-polymerpolyethylenimine to improve oral squamous carcinoma treatment. International Journal of Nanomedicine. 2018;13:187-98. https://doi.org/10.2147/IJN.S150610
Ngouangna EN, Manan MA, Oseh JO, Norddin MM, Agi A, Gbadamosi AO. Influence of (3-Aminopropyl) triethoxysilane on silica nanoparticle for enhanced oil recovery. Journal of Molecular Liquids. 2020;315:113740. http://dx.doi.org/10.1016/j.molliq.2020.113740
Alahmadi SM, Mohamad S, Maah MJ. Synthesis and characterization of mesoporous silica functionalized with calix [4] arene derivatives. International Journal of Molecular Sciences. 2012;13(10):13726-36. https://doi.org/10.3390/ijms131013726
Bchellaoui N, Hayat Z, Mami M, Dorbez-Sridi R, El Abed AI. Microfluidic-assisted formation of highly monodisperse and mesoporous silica soft microcapsules. Scientific Reports. 2017;7(1):16326. https://doi.org/10.1038/s41598-017-16554-4
Abou Rida AM, Harb F. Synthesis and characterization of amorphous silica nanoparitcles from aqueous silicates using cationic surfactants. Journal of Metals Materials and Minerals. 2014;24(1). Available from: https://jmmm.material.chula.ac.th/index.php/jmmm/article/view/108
Alves RH, Reis TVdS, Rovani S, Fungaro DA. Green synthesis and characterization of biosilica produced from sugarcane waste ash. Journal of Chemistry. 2017;4:1-9. https://doi.org/10.1155/2017/6129035
Hajiahmadi Z, Shirzadian-Khorramabad R, Kazemzad M, Sohani MM. Enhancement of tomato resistance to Tuta absoluta using a new efficient mesoporous silica nanoparticle-mediated plant transient gene expression approach. Scientia Horticulturae. 2019;243:367-75. https://doi.org/10.1016/j.scienta.2018.08.040
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