Quantitative evidence on reduction of CMD virus inoculum as influenced by management practices in cassava
DOI:
https://doi.org/10.14719/pst.5535Keywords:
Cassava mosaic disease, INM, IDM, DNA reduction, copy number, qPCR, Cq, viral loadAbstract
Cassava mosaic disease (CMD) poses a substantial challenge to the success of cassava cultivation in India, primarily attributed to the Indian cassava mosaic virus (ICMV) and the Sri Lankan cassava mosaic virus (SLCMV). This study examines the impact of diverse management techniques on diminishing CMD viral load in cassava plants, employing quantitative PCR (qPCR) as the principal measurement instrument. The experiment was conducted in a cassava field, with treatment plots assigned in a randomized block design (RBD). The research evaluated the effectiveness of different nutrient applications and insecticidal treatments. Results indicated that treatments, such as fish oil rosin soap and Cassava Booster, significantly reduced CMD viral concentrations 14 days post-application. The qPCR results showed a substantial decrease in virus copy numbers following these treatments. The study highlights the importance of integrated disease management strategies for CMD control, demonstrating how these practices can lead to healthier cassava crops, increased yields, and sustainable crop protection.
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Blagbrough IS, Bayoumi SAL, Rowan MG, Beeching JR. Cassava: An appraisal of its phytochemistry and its biotechnological prospects. Phytochemistry. 2010;71(17-18):1940-51. https://doi.org/10.1016/j.phytochem.2010.09.001
Indiastat. 2024. https://www.indiastat.com/data/agriculture/tapioca-cassava
Mathew AV. Studies on Indian cassava mosaic virus disease. Ph.D. Thesis. University of Agricultural Sciences, BANGALORE; 1988.
Edison S. Plant protection problems in cassava in India. In:Howeler RH.(Ed). Cassava Research and development in Asia: Exploring New Opportunities for an Ancient Crop. Proceedings of the Seventh Regional Workshop held in Bangkok, Thailand. 2002;264.
Fargette D, Jeger M, Fauquet C, Fishpool LDC. Analysis of temporal disease progress of African cassava mosaic virus. Phytopathology. 1993;84(1):91-98. https://doi.org/10.1094/Phyto-84-91
Fauquet C, Fargette D. African cassava mosaic virus: etiology, epidemiology and control. Plant Dis. 1990;74(6):404-11. https://doi.org/10.1094/PD-74-0404
Thurston HD. Tropical plant diseases [Internet]. American Phytopathological Society; 1998. https://books.google.co.in/books?id=BuI4AQAAIAAJ
Harrison BD, Robinson DJ. Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (begomoviruses). Annu Rev Phytopathol. 1999;37(1):369-98. https://doi.org/10.1146/annurev.phyto.37.1.369
Narasimhan V, Arjunan D. Effect of mosaic disease on cassava. Tapioca Research Station Salem Tamil Nadu India. 1974;10.
Thankappan M, Chacko CI. Effect of cassava mosaic on the different plant parts and tuber yield in cassava. J Root Crops.1976;2:45-47.
Nair NG, Malathi VG. Disease severity and yield loss in cassava (Manihot esculenta Crantz) due to Indian cassava mosaic disease. J Root Crops.1987;13(1): 91-94.
Owor B, Legg JP, Okao?Okuja G, Obonyo R, Ogenga?Latigo MW. The effect of cassava mosaic geminiviruses on symptom severity, growth and root yield of a cassava mosaic virus disease?susceptible cultivar in Uganda. Ann Appl Biol. 2004;145(3):331-37. https://doi.org/10.1111/j.1744-7348.2004.tb00390.x
Duraisamy R, Natesan S, Muthurajan R, Gandhi K, Lakshmanan P, Karuppusamy N, et al. Molecular studies on the transmission of Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV) in cassava by Bemisia tabaci and cloning of ICMV and SLCMV replicase gene from cassava. Mol Biotechnol. 2013;53:150-58. https://doi.org/10.1007/s12033-012-9503-1
Indra N, Kavitha PS, Deivamani M, Kavitha K, Chithra K, Kumar M, et al. Impact of integrated management practices against cassava mosaic disease in Tamil Nadu. Agr Mech Asia.2022;53(12):11037-11045
Abraham A. Tapioca Cultivation in India. Indian Council of Agricultural Research; 1956. https://books.google.co.in/books?id=DmisHAAACAAJ
Alagianagalingam MN, Ramakrishnan K. Cassava mosaic in India. South Ind Hort. 1966;14:71-72.
Anitha J, Makeshkumar T, Edison S. Potential hosts of Sri Lankan cassava mosaic virus evaluated through whitefly inoculation. J Root Crops. 2019;45(2):55-61.
Storey HH, Nichols RFW. Studies of the mosaic diseases of cassava. Ann Appl Biol. 1938;25(4):790-806. https://doi.org/10.1111/j.1744-7348.1938.tb02354.x
Thresh JM, Cooter RJ. Strategies for controlling cassava mosaic virus disease in Africa. Plant Pathol. 2005;54(5):587-614. https://doi.org/10.1111/j.1365-3059.2005.01282.x
Tembo M, Mataa M, Legg J, Chikoti PC, Ntawuruhunga P. Cassava mosaic disease: incidence and yield performance of cassava cultivars in Zambia. J Plant Pathol. 2017;99(3):681-89.
Harshitha R, Arunraj DR. Real?time quantitative PCR: A tool for absolute and relative quantification. Biochem Mol Biol Educ. 2021;49(5):800-12. https://doi.org/10.1002/bmb.21552
Lodhi MA, Ye GN, Weeden NF, Reisch BI. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol Biol Rep. 1994;12:6-13. https://doi.org/10.1007/BF02668658
Ehrt S, Schnappinger D. Isolation of Plasmids from E. coli by Alkaline Lysis. In: Casali N, Preston A.(eds). E. coli Plasmid Vectors.Methods in Molecular Biology™, 2003;235:75-78. Humana Press. https://doi.org/10.1385/1-59259-409-3:75
Wang C, Wang C, Zhang Z, Li X, Zhang T. Research note: Application of reverse-transcription recombinase-aided amplification-lateral flow dipstick method in the detection of infectious bursal disease virus. Poult Sci. 2023;102(1):102306. https://doi.org/10.1016/j.psj.2022.102306
Chellappan P, Vanitharani R, Fauquet CM. Short interfering RNA accumulation correlates with host recovery in DNA virus-infected hosts, and gene silencing targets specific viral sequences. J Virol. 2004;78(14):7465-77. https://doi.org/10.1128/JVI.78.14.7465-7477.2004
Ammara U, Al-Sadi AM, Al-Shihi A, Amin I. Real-time qPCR assay for the TYLCV titer in relation to symptoms-based disease severity scales. Int J Agric Biol. 2017;19(1):145-51.
Naseem S, Winter S. Quantification of African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV-UG) in single and mixed infected Cassava (Manihot esculenta Crantz) using quantitative PCR. J Virol Methods. 2016;227:23-32. https://doi.org/10.1016/j.jviromet.2015.10.001
Boopathi T, Karuppuchamy P, Kalyanasundaram M, Mohankumar S, Ravi M, Singh SB. Effect of botanicals, fish oil rosin soap and organic salt on eggs of spiralling whitefly, Aleurodicus dispersus. Indian J Plant Prot. 2014;42(1):86-88.
Neelakandan K, Karuppasami KM, Karuppusamy N, Shanmugam KP, Lakshmanan P, Subramanian S, et al. A combined nutrient/biocontrol agent mixture improve cassava tuber yield and cassava mosaic disease. Agronomy. 2021;11(8):1650. https://doi.org/10.3390/agronomy11081650
Gayathri M, Sharanya R, Renukadevi P, Nakkeeran S, Saranya N, Varanavasiappan S, et al. Deciphering the antiviral nature of endophytic Bacillus spp. against groundnut bud necrosis virus in cowpea and tomato. Front Microbiol. 2024;15:1410677. https://doi.org/10.3389/fmicb.2024.1410677
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Copyright (c) 2024 R Bhaargavi, TKS Latha, T Makeshkumar, S Harish, M Velmurugan, PA Saravanan
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