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Plant Science Today (PST)

Research Articles

Vol. 11 No. sp4 (2024): Recent Advances in Agriculture by Young Minds - I

Biopriming of seeds with microbial biostimulant (Bacillus megaterium ) on improvement of seedling growth, biochemical and root traits of rice (Oryza sativa L.)

DOI
https://doi.org/10.14719/pst.5541
Submitted
5 October 2024
Published
23-12-2024
Versions

Abstract

This study aimed to evaluate the potential of Bacillus megaterium in improving seed germination and rice seedling's morpho-physiological and biochemical traits. The experiment was conducted using a completely randomized design (CRD) to evaluate the effect of different concentrations of the biostimulant B. megaterium on rice seedlings growth and development under nursery conditions. Biopriming seeds with B. megaterium significantly enhanced seed germination and seedling traits, such as seedling shoot and root length, seedling height, number of leaves, seedling vigor, and shoot and root dry biomass, compared to the untreated control. Among the treatments, biopriming seeds with B. megaterium at a concentration of 10g kg-1 resulted in the greatest improvements across all the recorded parameters. Root traits such as total root length, surface area, average root diameter, root volume, and root tips and forks were also significantly enhanced. Additionally, biochemical changes like total chlorophyll and soluble protein
content showed notable improvement in B. megaterium biopriming with the concentration of 10g kg-1. Hence, these results suggest that biopriming seeds with B. megaterium are an efficient strategy to improve seed germination
and shoot and root vigor in rice seedlings.

References

  1. Tarantino A, Lops F, Disciglio G, Lopriore G. Effects of plant biostimulants on fruit set, growth, yield and fruit quality attributes of ‘Orange rubis®’apricot (Prunus armeniaca L.) cultivar in two consecutive years. Sci Hortic. 2018;239:26-34. https://doi.org/10.1016/j.scienta.2018.04.055
  2. Meddich A. Biostimulants for resilient agriculture—Improving plant tolerance to abiotic stress: A concise review. Gesunde Pflanzen. 2023;75:709-27. https://doi.org/10.1007/s10343-022-00784-2
  3. Rajput RS, Ram RM, Vaishnav A, Singh HB. Microbe-based novel biostimulants for sustainable crop production. In: Satyanarayana T, Das S, Johri B, editors. Microbial diversity in ecosystem sustainability and biotechnological applications. Singapore: Springer; 2019. p. 109-144. https://doi.org/10.1007/978-981-13-8487-5_5
  4. Li J, Van Gerrewey T, Geelen D. A meta-analysis of biostimulant yield effectiveness in field trials. Front Plant Sci. 2022;13:836702. https://doi.org/10.3389/fpls.2022.836702
  5. Di Y, Kui L, Singh P, Liu LF, Xie LY, He LL, et al. Identification and characterization of Bacillus subtilis B9: A diazotrophic plant growth-promoting endophytic bacterium isolated from sugarcane root. J Plant Growth Regul. 2022;42:1720-37. https://doi.org/10.1007/s00344-022-10653-x
  6. Narayanasamy S, Thankappan S, Kumaravel S, Ragupathi S, Uthandi S. Complete genome sequence analysis of a plant growth-promoting phylloplane Bacillus altitudinis FD48 offers mechanistic insights into priming drought stress tolerance in rice. Genomics. 2023;115(1):110550. https://doi.org/10.1016/j.ygeno.2022.110550
  7. Gaur P, Arora S, Prakash V, Sood G. Bioprospecting of multi trait plant growth promoting Bacillus altitudinis from phosphate rich soil. Geomicrobiol J. 2023;40(3):255-63. https://doi.org/10.1080/01490451.2022.2155889
  8. Kang SM, Radhakrishnan R, You YH, Joo GJ, Lee IJ, Lee KE, et al. Phosphate solubilizing Bacillus megaterium mj1212 regulates endogenous plant carbohydrates and amino acids contents to promote mustard plant growth. Indian J Microbiol. 2014;54:427-33. https://doi.org/10.1007/s12088-014-0476-6
  9. Wang S, Na X, Yang L, Liang C, He L, Jin J, et al. Bacillus megaterium strain WW1211 promotes plant growth and lateral root initiation via regulation of auxin biosynthesis and redistribution. Plant Soil. 2021;466:491-504. https://doi.org/10.1007/s11104-021-05055-z
  10. Widnyana IK, Javandira C. Activities Pseudomonas spp. and Bacillus sp. to stimulate germination and seedling growth of tomato plants. Agric Agric Sci Procedia. 2016;9:419-23. https://doi.org/10.1016/j.aaspro.2016.02.158
  11. Murali M, Singh SB, Gowtham HG, Shilpa N, Prasad M, Aiyaz M, et al. Induction of drought tolerance in Pennisetum glaucum by ACC deaminase producing PGPR- Bacillus amyloliquefaciens through antioxidant defense system. Microbiol Res. 2021;253:126891. https://doi.org/10.1016/j.micres.2021.126891
  12. Hu Q, Xiao Y, Liu Z, Huang X, Dong B, Wang Q. Bacillus subtilis QM3, a plant growth-promoting rhizobacteria, can promote wheat seed germination by gibberellin Pathway. J Plant Growth Regul. 2024;43:2682–2695. https://doi.org/10.1007/s00344-024-11298-8
  13. Kumari S, Kumar P, Kiran S, Kumari S, Singh A. Characterization of culture condition dependent, growth responses of phosphate solubilizing bacteria (Bacillus subtilis DR2) on plant growth promotion of Hordeum vulgare. Vegetos. 2024;37:266-76. https://doi.org/10.1007/s42535-023-00589-2
  14. Miljakovic D, Marinkovic J, Tamindžic G, Dordevic V, Ignjatov M, Miloševic D, et al. Effect of plant growth promoting Bacillus spp. on germination and seedling growth of soybean. Legume Res. 2022;45(4):487-91. https://doi.org/10.18805/lrf-665
  15. Song P, Zhao B, Sun X, Li L, Wang Z, Ma C, et al. Effects of Bacillus subtilis HS5B5 on maize seed germination and seedling growth under NaCl stress conditions. Agronomy. 2023;13(7):1874. https://doi.org/10.3390/agronomy13071874
  16. Rajkumar M, Narayanasamy S, Uthandi S. A root-associated Bacillus albus LRS2 and its metabolites for plant growth promotion and drought stress tolerance in little millet (Panicum sumatrense L.). Plant Stress. 2024;12:100446. https://doi.org/10.1016/j.stress.2024.100446
  17. Selvaraj A, Thangavel K, Uthandi S. Arbuscular mycorrhizal fungi (Glomus intraradices) and diazotrophic bacterium (Rhizobium BMBS) primed defense in blackgram against herbivorous insect (Spodoptera litura) infestation. Microbiol Res. 2020;231:126355. https://doi.org/10.1016/j.micres.2019.126355
  18. Rosier A, Pomerleau M, Beauregard PB, Samac DA, Bais HP. Surfactin and Spo0A-dependent antagonism by Bacillus subtilis strain UD1022 against Medicago sativa phytopathogens. Plants. 2023;12(5):1007. https://doi.org/10.3390/plants12051007
  19. Dutta B, Datta A, Dey A, Ghosh AK, Bandopadhyay R. Establishment of seed biopriming in salt stress mitigation of rice plants by mangrove derived Bacillus sp. Biocatal Agric Biotechnol. 2023;48:102626. https://doi.org/10.1016/j.bcab.2023.102626
  20. Bora K. Spatial patterns of fertilizer use and imbalances: Evidence from rice cultivation in India. Environ Chall. 2022;7:100452. https://doi.org/10.1016/j.envc.2022.100452
  21. Beerelli K, Balakrishnan D, Surapaneni M, Addanki K, Rao YV, Neelamraju S. Evaluation of yield and seedling vigour related traits of Swarna/Oryza nivara backcross introgression lines under three environment conditions. Ecol Genet Genom. 2019;11:100036. https://doi.org/10.1016/j.egg.2019.100036
  22. Mishra A, Salokhe VM. Seedling characteristics and the early growth of transplanted rice under different water regimes. Exp Agric. 2008;44(3):365-83. https://doi.org/10.1017/S0014479708006388
  23. Li YX, Liu Y, Wang YH, Ding YF, Wang SH, Liu ZH, et al. Effects of seedling age on the growth stage and yield formation of hydroponically grown long-mat rice seedlings. J Integr Agric. 2020;19(7):1755-67. https://doi.org/10.1016/s2095-3119(19)62756-5
  24. Narayanasamy S, Uthandi S. Impact of moisture stress and Bacillus altitudinis FD48 on physiological modulation and seed germination in rice (Oryza sativa L.). Madras Agric J. 2020;107:53-60. https://doi.org/10.29321/MAJ.2020.000331
  25. Al-Hazmi NE, Naguib DM. Amylase properties and its metal tolerance during rice germination improved by priming with rhizobacteria. Rhizosphere. 2022;22:100518. https://doi.org/10.1016/j.rhisph.2022.100518
  26. Musa SI, Ikhajiagbe B. Seed bio-priming with phosphate-solubilizing bacteria strains to improve rice (Oryza sativa L. var. FARO 44) growth under ferruginous ultisol conditions. BioTechnologia. 2023;104(1):33-51. https://doi.org/10.5114/bta.2023.125084
  27. González-Naharro R, Quirós E, Fernández-Rodríguez S, Silva-Palacios I, Tormo-Molina R, Maya-Manzano JM, et al. Spearman correlation between the NDVI and quercus airborne pollen in the SW of the Iberian Peninsula. Proceedings. 2018;2(20):1519. https://doi.org/10.3390/proceedings2201519
  28. Pandey R, Chinnusamy V, Rathod G, Paul V, Jain N. Evaluation of root growth and architecture. In: Manual of ICAR sponsored training programme on physiological techniques to analyze the impact of climate change on crop plants; 2017 Jan 16-25; New Delhi. Division of Plant Physiology, IARI; 2017. p. 95-102.
  29. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75. https://doi.org/10.1016/S0021-9258(19)52451-6
  30. Hiscox JD, Israelstam GF. A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot. 1979;57(12):1332-34. https://doi.org/10.1139/b79-163
  31. Ng L, Sariah M, Sariam O, Radziah O, Zainal Abidin MA. Rice seed bacterization for promoting germination and seedling growth under aerobic cultivation system. Aust J Crop Sci. 2012;6(1):170-75. https://doi/10.3316/informit.054006592911274
  32. Rajer FU, Samma MK, Ali Q, Rajar WA, Wu H, Raza W, et al. Bacillus spp.-mediated growth promotion of rice seedlings and suppression of bacterial blight disease under greenhouse conditions. Pathogens. 2022;11(11):1251. https://doi.org/10.3390/pathogens11111251
  33. Chen G, Weil RR. Root growth and yield of maize as affected by soil compaction and cover crops. Soil Tillage Res. 2011;117:17-27. https://doi.org/10.1016/j.still.2011.08.001
  34. Verbon EH, Liberman LM. Beneficial microbes affect endogenous mechanisms controlling root development. Trends Plant Sci. 2016;21(3):218-29. https://doi.org/10.1016/j.tplants.2016.01.013
  35. Moretti LG, Crusciol CAC, Kuramae EE, Bossolani JW, Moreira A, Costa NR, et al. Effects of growth-promoting bacteria on soybean root activity, plant development and yield. Agron J. 2020;112(1):418-28. https://doi.org/10.1002/agj2.20010
  36. Wang P, Grimm B. Connecting chlorophyll metabolism with accumulation of the photosynthetic apparatus. Trends Plant Sci. 2021;26(5):484-95. https://doi.org/10.1016/j.tplants.2020.12.005
  37. Ragadevi K, Jeyakumar P, Djanaguiraman M, Kalaiselvi T, Arul L, Mahalingam L, et al. Seed biopriming improved growth and morpho-physiological traits in early vegetative phase of compact cotton. Biol Forum. 2021;13(4):1082-88.
  38. Li H, Yue H, Li L, Liu Y, Zhang H, Wang J, et al. Seed biostimulant Bacillus sp. MGW9 improves the salt tolerance of maize during seed germination. AMB Express. 2021;11:74. https://doi.org/10.1186/s13568-021-01237-1

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