Unveiling genetic diversity in teak (Tectona grandis L. f.) accessions through ISSR profiling

M N Ashwath; A V Santhoshkumar; C Shankar; E Manju; M Deepu; K Shiran

doi:10.14719/pst.5745

Authors

M N Ashwath College of Agriculture, Gangavathi, University of Agricultural Sciences, Raichur, Karnataka -583 227, India https://orcid.org/0000-0002-4041-8343
College of Forestry, Kerala Agricultural University, Thrissur, Kerala 680 656, India https://orcid.org/0000-0003-4253-8581
C Shankar Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, Kerala 680 656, India https://orcid.org/0009-0009-2535-5987
E Manju College of Forestry, Kerala Agricultural University, Thrissur, Kerala 680 656, India https://orcid.org/0000-0003-2731-3501
M Deepu Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, Kerala 680 656, India https://orcid.org/0000-0002-2941-1060
K Shiran Indian Council of Agricultural Research- Central Arid Zone Research Institute, Jodhpur-342 003, India

DOI:

https://doi.org/10.14719/pst.5745

Keywords:

diversity, evolution, molecular-markers, population, teak

Abstract

The present study focuses on understanding the genetic diversity among the selected teak accessions using Inter simple sequence repeat (ISSR) markers. Genomic DNA from ten selected teak accessions was extracted using a modified Cetyltrimethylammonium Bromide (CTAB) protocol, ensuring high quality and yield. ISSR primers were employed to amplify DNA fragments and resulting bands were visualized. Banding patterns were analyzed to determine the presence of alleles and polymorphism. The study revealed significant genetic diversity among teak accessions, with 212 alleles identified, of which 199 were polymorphic. Cluster analysis grouped accessions into three distinct clusters based on genetic relatedness, highlighting the influence of environmental factors and geographic location on teaks’ genetic variability. Cluster I comprised two accessions, A1 (Top slip-6) and A2 (Top slip-10) (Jaccards’ coefficient=0.361). Notably, A6 (Nellikutha-1) and A4 (Nedumkayam-2) show a close genetic relationship, sharing a common ancestry with A10 (Karulai) and finally, all three are linked with A7 (Nedumkayam-1), forming cluster II. Cluster III included two pairs of closely related accessions: A3(TC4)-A5(TC11) and A8(Nellikutha-2) - A9 (Shankaram-thode1). Neis’ genetic identity and distance indices provided further insights into the degree of genetic similarity and differentiation among accessions.

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