Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 11 No. sp4 (2024): Recent Advances in Agriculture by Young Minds - I

Insecticidal activity and phytochemical profiling of Annona squamosa (L.) leaf extract against the fall armyworm, Spodoptera frugiperda (J.E. Smith)

DOI
https://doi.org/10.14719/pst.5770
Submitted
13 October 2024
Published
31-12-2024

Abstract

To identify an effective botanical extract for pest control, six locally available plant species were screened for their toxic effects against Spodoptera frugiperda (J.E. Smith) larvae. These species included Annona squamosa (L.), Calotropis gigantea (L.) W.T. Aiton, Carica papaya (L.), Datura metel (L.), Ricinus communis (L.) and Rottboellia cochinchinensis (Lour.) Clayton. Among these, A. squamosa demonstrated the highest larval mortality at both 5% and 10% concentrations. Based on these promising results, subsequent bioassays were conducted using a range of concentrations (4%, 6%, 8%, 10%, 12%, 14% and 16%) of A. squamosa to evaluate its larvicidal, antifeedant and ovicidal activities. The median lethal concentration (LC50) for second instar larvae, observed 72 hours after treatment (HAT), was determined to be 8.65% (w/v). The antifeedant assay revealed a dose-dependent response, with 99.38% antifeedancy at the highest concentration tested (16%). Moreover, A. squamosa exhibited 100% ovicidal activity across all concentrations. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the extract identified 39 compounds, with 16-hentriacontanone, neophytadiene, caryophyllene and phytol showing the most significant peak areas. These findings suggest that A. squamosa leaf extract possesses substantial larvicidal, antifeedant and ovicidal properties against S. frugiperda, highlighting its potential as an eco-friendly, non-toxic alternative to synthetic insecticides. This botanical extract may offer valuable contributions to integrated pest management (IPM) strategies, promoting sustainable agricultural practices.

References

  1. Asokan R, Swamy HM, Maruthi MS, Pavithra HB, Hegde K, Nave S, et al. First report of the fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), an alien invasive pest on maize in India. Pest Manage Horti Ecosyst. 2018;17:24(1).
  2. Rioba NB, Stevenson PC. Opportunities and scope for botanical extracts and products for the management of fall armyworm (Spodoptera frugiperda) for smallholders in Africa. Plants. 2020;9(2):207. https://doi.org/10.3390/plants902020
  3. Suby S, Soujanya PL, Yadava P, Patil J, Subaharan K, Prasad GS, et al. Invasion of fall armyworm (Spodoptera frugiperda) in India. Curr Sci. 2020;119(1):44-51. https://doi.org/10.18520/cs/v119/i1/44-51
  4. Mooventhan P, Murali BRK, Sridhar J, Kaushal P, Kumar J. Technical bulletin on integrated management of fall armyworm in maize (NIBSM/TB/2019-01), ICAR- National Institute of Biotic Stress Management, India; 2019. Available from: https://nibsm.icar.gov.in/images/Technical-Bulletin-Integrated-Management-of-FallArmyworm-in-Maize.pdf
  5. Deshmukh SS, Prasanna B, Kalleshwaraswamy C, Jaba J, Choudhary B. Fall armyworm (Spodoptera frugiperda). polyphagous pests of crops. Springer: Singapore; 2021. https://doi.org/10.1007/978-981-15-8075-8_8
  6. Nidhi K, Joshi M, Pandey R, Anand K. Fall army worm: an invasive pest in India and its management. J Entomol Zool Stud. 2019;7(5):1034-37.
  7. Valladares-Cisneros MG, Rios-Gomez MY, Aldana-Llanos L, ValdesEstrada ME, Ochoa MG. Biological activity of Crescentia alata (Lamiales: Bignoniaceae) fractions on larvae of Spodoptera frugiperda (Lepidoptera: Noctuidae). Fla Entomol. 2014;97(2):770-77. https://doi.org/10.1653/024.097.0259
  8. Mendesil E, Tefera T, Blanco CA, Paula-Moraes SV, Huang F, Viteri DM, Hutchison WD. The invasive fall armyworm, Spodoptera frugiperda, in Africa and Asia: responding to the food security challenge, with priorities for integrated pest management research. J Plant Dis Prot. 2023;130(6):1175-206. https://doi.org/10.1007/s41348-023-00777-x
  9. Zhang P, Zhou Y, Qin D, Chen J, Zhang Z. Metabolic changes in larvae of predator Chrysopa sinica fed on azadirachtin-treated Plutella xylostella larvae. Metabolites. 2022;12(2):158. https://doi.org/10.3390/metabo12020158
  10. Ribeiro SS, Silva TBD, Moraes VRDS, Nogueira PCDL, Costa EV, Bernardo AR, et al. Chemical constituents of methanolic extracts of Jatropha curcas L and effects on Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Quim Nova. 2012;35:2218-21. https://doi.org/10.1590/S0100-40422012001100022
  11. Salinas-Sánchez DO, Aldana-Llanos L, Valdés-Estrada ME, GutiérrezOchoa M, Valladares-Cisneros G, Rodríguez-Flores E. Insecticidal activity of Tagetes erecta extracts on Spodoptera frugiperda (Lepidoptera: Noctuidae). Fla Entomol. 2012;95(2):428-32. https://doi.org/10.1653/024.095.0225
  12. Sisay B, Tefera T, Wakgari M, Ayalew G, Mendesil E. The efficacy of selected synthetic insecticides and botanicals against fall armyworm, Spodoptera frugiperda, in maize. Insects. 2019;10(2):45. https://doi.org/10.3390/insects10020045
  13. Jiménez-Durán A, Barrera-Cortés J, Lina-García LP, Santillan R, Soto-Hernández RM, Ramos-Valdivia AC, et al. Biological activity of phytochemicals from agricultural wastes and weeds on Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae). Sustainability. 2021;13(24):13896. https://doi.org/10.3390/su132413896
  14. Alves APC, Corrêa AD, Alves DS, Saczk AA, Lino JB, Carvalho GA. Toxicity of the phenolic extract from jabuticabeira (Myrciaria cauliflora (Mart.) O. Berg) fruit skins on Spodoptera frugiperda. Chil J Agric Res. 2014;74(2):200-04. https://doi.org/10.4067/S0718-58392014000200011
  15. Tefera T. Mass rearing of stem borers, maize weevil and larger grain borer insect pests of maize: CIMMYT; 2010.
  16. Moadeli T, Mainali B, Ponton F, Taylor PW. Effects of fatty acids and vitamin E in larval diets on development and performance of Queensland fruit fly. J Insect Physiol. 2020;125:104058. https://doi.org/10.1016/j.jinsphys.2020.104058
  17. Hegde DR, Nelson SJ, Natarajan N, Mohan Kumar S, Arumugam T. A study on growth and development of brinjal shoot and fruit borer, Leucinodes orbonalis (Guenee) on different artificial diets. J Entomol Zool Stud. 2018;6(1):555-59.
  18. Phambala K, Tembo Y, Kasambala T, Kabambe VH, Stevenson PC, Belmain SR. Bioactivity of common pesticidal plants on fall armyworm larvae (Spodoptera frugiperda). Plants. 2020;9(1):112. https://doi.org/10.3390/plants9010112
  19. Kalinda RS, Rioba NB. Phytochemical analysis and efficacy of rosemary and mint extracts against fall armyworm (Spodoptera frugiperda) on baby corn (Zea mays). Acta Chemica Malaysia. 2020;4(2):66-71. https://doi.org/10.2478/acmy-2020-0011
  20. Gupta G, Sharma S, Kumar N. Carica papaya aqueous leaf extracts as potential botanical insecticide against rose aphids (Macrosiphum rosaeformisD.). J Entomol Zool Stud. 2020;8:960-64.
  21. Kardinan A, Maris P, editors. In: Effect of botanical insecticides against fall armyworm Spodoptera frugiperda JE Smith (Lepidoptera: Noctuidae). IOP Conference Series: Earth and Environmental Science; 2021: IOP Publishing. https://doi.org/10.1088/1755-1315/653/1/012060
  22. Karunamoorthi K. Medicinal and aromatic plants: a major source of green pesticides/risk-reduced pesticides. Medicinal and Aromatic Plants. 2012;1(8):2167-412. https://doi.org/10.4172/2167-0412.1000e137
  23. Finney DJ, Stevens WL. A table for the calculation of working probits and weights in probit analysis. Biometrika. 1948 May 1;35(1/2):191-201. https://doi.org/10.1093/biomet/35.1-2.191
  24. Nyirenda J, Kadango Z, Funjika E, Chipabika G. Larvicidal, ovicidal and antifeedant activity of crude cashew nutshell liquid against fall armyworm, Spodoptera frugiperda (JE smith), (Lepidoptera, Noctuidae). Crop Prot. 2024;179:106619. https://doi.org/10.1016/j.cropro.2024.106619
  25. Pan L, Ren L, Chen F, Feng Y, Luo Y. Antifeedant activity of Ginkgo biloba secondary metabolites against Hyphantria cunea larvae: mechanisms and applications. PLoS One. 2016;11(5):e0155682. https://doi.org/10.1371/journal.pone.0155682
  26. Singh R, Pant N. Hymenocallis littoralis Salisb. as antifeedant to desert locust Schistocerca gregaria Forsk; 1981.
  27. de Souza TW, Cruz I, Petacci F, de Assis JSL, de Sousa FS, Zanuncio JC, Serrão JE. Potential use of Asteraceae extracts to control Spodoptera frugiperda (Lepidoptera: Noctuidae) and selectivity to their parasitoids Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) and Telenomus remus (Hymenoptera: Scelionidae). Ind Crops Prod. 2009 Nov 1;30(3):384-88. https://doi.org/10.1016/j.indcrop.2009.07.007
  28. Shiragave P. Effect of crude extract of Atalantia monophylla L. on ovicidal activity against Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) and preliminary phytochemical study. J Entomol Zool Stud. 2018;6(1):1744-46.
  29. Zeb A, Ullah F, Ayaz M, Ahmad S, Sadiq A. Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GCMS analysis in the ethyl acetate extract. BMC Complement Altern Med. 2017;17:1-16. https://doi.org/10.1186/s12906-017-1798-9
  30. Pérez-Gutiérrez S, Zavala-Sánchez MA, González-Chávez MM, Cárdenas-Ortega NC, Ramos-López MA. Bioactivity of Carica papaya (Caricaceae) against Spodoptera frugiperda (lepidoptera: Noctuidae). Molecules. 2011;16(9):7502-09. https://doi.org/10.3390/molecules16097502
  31. Ramos-López M, González-Chávez M, Cárdenas-Orteg N, ZavalaSánchez M. Activity of the main fatty acid components of the hexane leaf extract of Ricinus communis against Spodoptera frugiperda. Afr J Biotechnol. 2012;11(18):4274-78. https://doi.org/10.5897/AJB11.3727
  32. Aprialty A, Sjam S, Dewi V, Agustina Y, editors. In: The synergy of Calotropis gigantea and Cresscentia cujete plant extracts as an inhibitor of egg hatching and antifeedant against Spodoptera frugiperda. IOP Conference Series: Earth and Environmental Science; 2021: IOP Publishing. https://doi.org/10.1088/1755-1315/807/2/022086
  33. Henagamage A, Ranaweera M, Peries C, Premetilake M. Repellent, antifeedant and toxic effects of plants-extracts against Spodoptera frugiperda larvae (fall armyworm). Biocatal Agric Biotechnol. 2023;48:102636. https://doi.org/10.1016/j.bcab.2023.102636
  34. Muthu R, Vishnupriya R, Nelson SJ, Uma D, SanthanaKrishnan V. Insect growth inhibitory activity of Annona squamosa L. against polyphagous insect pest, Spodoptera litura F. Indian J Entomol. 2023; 86(3), 956-59. https://doi.org/10.55446/IJE.2023.1313
  35. Chan KN, Khaing AA, Phyoe A, Thida M, Myo WW, Soe AT, et al. Bioefficacy of Annona squamosa, Azadirachta indica and Artocarpus heterophyllus against fall armyworm. Bull Insectology. 2023;76.
  36. Casamina CJE, Reyes DF. Extracts of Senna sophera (L.) Roxb., Syzygium cumuni and Annona squamosa Linn. as biopesticides against army worm (Spodoptera exigua) Larvae. Oriental Journal of Chemistry. 2022;38(2):427. https://doi.org/10.13005/ojc/380226
  37. Khalequzzaman M, Sultana S. Insecticidal activity of Annona squamosa L. seed extracts against the red flour beetle, Tribolium castaneum (Herbst). J Biosci. 2006;14:107-12. https://doi.org/10.3329/jbs.v14i0.453
  38. Leatemia JA, Isman MB. Insecticidal activity of crude seed extracts of Annona spp., Lansium domesticum and Sandoricum koetjape against lepidopteran larvae. Phytoparasitica. 2004 Feb;32:30-37. https://doi.org/10.1007/BF02980856
  39. de Souza CM, Baldin ELL, do Prado Ribeiro L, dos Santos TLB, da Silva IF, Morando R, et al. Antifeedant and growth inhibitory effects of Annonaceae derivatives on Helicoverpa armigera (Hübner). Crop Prot. 2019;121:45-50. https://doi.org/10.1016/j.cropro.2019.03.008
  40. Kulkarni N, Joshi K, Shukla P. Antifeedant activity of Annona squamosa Linn. against Crypsiptya coclesalis Walker (Lepidoptera: Pyralidae). EntomonTrivandrum. 2003;28:389-92.
  41. Tavares WdS, Grazziotti GH, Júnior AADS, Freitas SDS, Consolaro HN, Ribeiro PEDA, et al. Screening of extracts of leaves and stems of Psychotria spp.(Rubiaceae) against Sitophilus zeamais (Coleoptera: Curculionidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae)for maize protection. J Food Prot. 2013;76(11):1892-901. https://doi.org/10.4315/0362-028X.JFP-13-123
  42. Sombra KE, de Aguiar CV, de Oliveira SJ, Barbosa MG, Zocolo GJ, Pastori PL. Potential pesticide of three essential oils against Spodoptera frugiperda (JE Smith)(Lepidoptera: Noctuidae). Chil J Agricu Res. 2020;80(4):617-28. https://doi.org/10.4067/S0718-58392020000400617
  43. Abdelgaleil SA, Mohamed MI, Badawy ME, El-arami SA. Fumigant and contact toxicities of monoterpenes to Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase activity. J Chem Ecol. 2009;35:518-25. https://doi.org/10.1007/s10886-009-9635-3
  44. Cruz-Estrada A, Gamboa-Angulo M, Borges-Argáez R, Ruiz-Sánchez E. Insecticidal effects of plant extracts on immature whitefly Bemisia tabaci Genn.(Hemiptera: Aleyroideae). Electron J Biotechnol. 2013;16(1):6. https://doi.org/10.2225/vol16-issue1-fulltext-6
  45. Ma S, Jia R, Guo M, Qin K, Zhang L. Insecticidal activity of essential oil from Cephalotaxus sinensis and its main components against various agricultural pests. Ind Crops Prod. 2020;150:112403. https://doi.org/10.1016/j.indcrop.2020.112403
  46. Sotelo-Leyva C, Toledo-Hernández E, Salinas-Sánchez DO, AvilésMontes D, Silva-Aguayo GI, Hernández-Velázquez VM, et al. Insecticidal potential of pure commercial molecules against the sugarcane aphid, Melanaphis sacchari (Zehntner) 1. Southwest Entomol. 2023;47(4):845-52. https://doi.org/10.3958/059.047.0406

Downloads

Download data is not yet available.