Standardization of seed testing procedure techniques in Sunnhemp (Crotalaria juncea) var ADT 1
DOI:
https://doi.org/10.14719/pst.6088Keywords:
germination, grading, physical purity, seed quality, seed testing, viabilityAbstract
This abstract presents a comprehensive overview of seed testing procedures techniques specifically designed for sunnhemp (Crotalaria juncea) var ADT 1, an important leguminous plant exploited for its versatile uses in agriculture and various industries. The seed testing procedure comprises a systematic approach to evaluate the quality, viability, and germination potential of sunnhemp seeds, crucial for ensuring successful crop establishment and yield optimization. Seed testing procedure viz., test weight determination, sample size estimation, purity assessment, standardization of germination media, temperature requirement for germination and determination of first count, final count was highlighted. The experiment results revealed that submitted sample size could be 700g, while the working sample size was be 70g and minimum standards for the pure seed and the inert matter percentage were found to be 98 and 1.5 per cent respectively. In seed germination test, paper medium with roll towel registered maximum germination percentage (96%) and vigour index (2146). Similarly, 30oC temperature was registered maximum germination percentage. Based on this result the first count and final count was fixed as 3rd and 10th day in sunnhemp var ADT 1. Thus, seed testing procedures contributes to advancement in the evaluation of sunnhemp seed quality, thereby promoting sustainable agriculture and economic prosperity.
Downloads
References
Bhattacharyya R, Ghosh BN, Mishra PK, Mandal B, Rao CS, Sarkar D, Franzluebbers AJ. Soil degradation in India: challenges and potential solutions. Sustain. 2015;7(4):3528–70. https://doi.org/10.3390/su7043528
Iderawumi AM, Kamal TO. Green manure for agricultural sustainability and improvement of soil fertility. Farming and Management. 2022;7(1):1–8. 10.31830/2456-8724.2022.fm-101
Kumar V, Pawar K. A review on soil health and fertility management in organic agriculture through green manuring. J Pharmacogn Phytochem. 2018;7(1S): 3213–17.
Bagli SB, Gowda B, Shakuntala NM, Doddagoudar SR, Sunkad G, Meena MK. Standardization of nipping technique for enhancement of seed yield and quality in sunn hemp. Int J Plant Sci. 2023;35(18):952–70. https://doi.org/10.9734/ijpss/2023/v35i183360
Castellano-Hinojosa A, Mora C, Strauss SL. Native rhizobia improve plant growth, fix n2 and reduce greenhouse emissions of sunnhemp more than commercial rhizobia inoculants in Florida citrus orchards. Plants. 2022;11(22):3011. https://doi.org/10.3390/plants11223011
Kamireddy SR, Li J, Abbina S, Berti M, Tucker M, Ji Y. Converting forage sorghum and sunn hemp into biofuels through dilute acid pretreatment. Ind Crop Prod. 2013; 49:598–609. https://doi.org/10.1016/j.indcrop.2013.06.018
Colegate SM, Gardner DR, Joy RJ, Betz JM, Panter KE, Chemistry F. Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn Hemp cv.Tropic Sun). J Agric Food Chem Mar. 2012;60(14):3541–50. https://doi.org/10.1021/jf205296s
Collins AS, Chase CA, Stall WM, Hutchinson CM. Optimum densities of three leguminous cover crops for suppression of smooth pigweed (Amaranthus hybridus). Weed Sci. 2008; 56(5):753–61. https://doi.org/10.1614/ws-07-101.1
Bundit A, Ostlie M, Prom-U-Thai. Sunn hemp (Crotalaria juncea) weed suppression and allelopathy at different timings. Biocontrol Sci Technol. 2021;31(7):694–704. https://doi.org/10.1080/09583157.2021.1881446
Kavin S, Subrahmaniyan K, Mannan SK. Optimization of plant geometry and NPK levels for seed and fibre yield maximization in sunnhemp [Crotalaria juncea (L.)] genotypes. Madras Agric J. 2018;105:1–3. https://doi.org/10.29321/maj.2018.000121
Sundareswaran S, Choudhury RP, Vanitha C, Yadava DK. Seed quality: Variety development to planting–An overview. In: Dadlani M, Yadava DK, Editors. Seed science and technology. Singapore: Springer; 2023. p. 1–16 https://doi.org/10.1007/978-981-19-5888-5_1 2023:1-16. Available form https://link.springer.com/chapter/10.1007/978-981-19-5888-5_1
Arun K, Sujatha K, Geetharani P, Renganayaki P, Sundareswaran S. Standardization of quick viability and test weight protocol for Moringa oleifera L. var. PKM. J Pharm Innov. 2021;10(10):2547–49. Available form: https://www.thepharmajournal.com/archives/2021/vol10issue10/PartAJ/10-10-376-252.pdf
ISTA. International rules for seed testing [internet]. Bassersdorf Switzerland: International Seed Testing Association; 2020 [cited 2024 Oct 10] Available from: https://www.seedtest.org/
Kumar B, Verma AK, Ram G, Singh HP, Lal RK. Genotype-independent germination-testing procedure for Isabgol. J New Seeds. 2009;10(3):196?205. https://doi.org/10.1080/15228860903115211
Panse VC, Sukhatme PV. Statistical methods for agricultural workers. New Delhi: ICAR; 1978 Available form: https://www.scirp.org/reference/referencespapers?referenceid=1814819
Navamaniraj N. Research focus on seed handling techniques in Bixa orellana L. [Thesis]. Coimbatore: Tamil Nadu Agricultural University; 2005
Justice OL, Kozlowski TT. Essentials of seed testing. In: Kozlowski TT, editor. Seed biology. vol. 3. New York: Academic Press; 1972 p. 301–70. https://doi.org/10.1016/B978-0-12-395605-7.50011-9
Chauhan R, Chauhan JS. Standardization on seed testing protocols and seed quality parameters in medicinal plants Embelia tsjeriam-cottam and Trichosanthes tricuspidata. J Pharma Phytochem. 2020;9(2):87–93. Available form: https://www.phytojournal.com/archives/2020/vol9issue2/PartB/9-1-514-232.pdf
Iyorkaa N, Omoigui L, Odo PE, Kamara A, Ugbaa MS, Ekeuro GC. Physical purity of cowpea seeds from trained community based and licensed seed producers in three states of Nigeria. Biosci Bioengin. 2021;6(2):10–19. Available form: https://cgspace.cgiar.org/items/fc45aab2-fa3c-45ee-a123-ed10a27d4098
Thangjam U, Sahoo UK. Effects of different pre-treatments and germination media on seed germination and seedling growth of Parkia timoriana (DC.) Merr. J Exp Bio. 2017;5(1):98–105. https://doi.org/10.18006/2017.5(1).098.105.
Taufiq HRS, Ridhawati A. The vigor and viability seed testing of three tobacco varieties on various seed germination media. Agrotech J. 2020;5(1):1–9. https://doi.org/10.31327/atj.v5i1.1210
Dubal I, Troyjack C, Koch F, Aisenberg G, Szareski VJ, Pimentel JR. Effect of temperature on bean seed germination, vigor and isozyme expression. Am J Agric Res. 2016;1(5):1–9. Available form: https://www.researchgate.net/publication/317007879_Effect_of_temperature_on_bean_seed_germination_vigor_and_isozyme_expression
Khaeim H, Kende Z, Jolánkai M, Kovács GP, Gyuricza C, Tarnawa Á. Impact of temperature and water on seed germination and seedling growth of maize (Zea mays L.). Agron. 2022;12(2):397. https://doi.org/10.3390/agronomy12020397
Sghaier HA, Tarnawa Á, Khaeim H, Kovács GP, Gyuricza C, Kende Z. The effects of temperature and water on the seed germination and seedling development of rapeseed (Brassica napus L.). Plants. 2022;11(21):2819. https://doi.org/10.3390/plants11212819

Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 P Kaviyarasu, K Sujatha, V Alex Albert, T Ragavan, G Anand

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).