The resultant seed quality of harvested seeds from elevated CO2 condition over a prolonged period of storage has not been widely investigated. Seed deterioration during storage is highly undesirable and poses significant challenges to agriculture. This process involves a range of physical, physiological, and biochemical changes in seeds, which can lead to reduced viability and germination rates. These changes are often caused by inadequate or improper storage conditions, which fail to provide the necessary systematic approaches to preserve seed quality over time.The study was conducted to evaluate the impact of different storage conditions on the seeds of rice cultivar CO 51 grown in elevated CO2 condition. Rice seeds were packed in different packaging materials viz., gunny bag, polythene bag and plastic container stored in two environments: ambient storage (27º ± 2ºC/ CO2 400ppm) and elevated CO2 (550 ppm). The result revealed that the decline in physiological seed quality, membrane damage, impairment of biosynthetic reactions and reduced the antioxidant defence system, which leads to seed deterioration with prolonged storage at ambient condition. Among the storage environments, the seeds stored in elevated CO2 condition are ideal to maintain seed viability and biochemical properties of the seeds with least deterioration. Our results suggest that the elevated CO2 which help maintain seed longevity during storage until the next season.